Chandipura virus (strain I653514) (CHPV)
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9WH76|MATRX_CHAV Matrix protein OS=Chandipura virus (strain I653514) OX=11273 GN=M PE=1 SV=1
MM1 pKa = 7.99 EE2 pKa = 6.5 DD3 pKa = 3.3 SQLYY7 pKa = 9.15 QALKK11 pKa = 10.46 NYY13 pKa = 8.16 PKK15 pKa = 10.79 LQDD18 pKa = 3.48 TLDD21 pKa = 4.06 SIEE24 pKa = 4.29 NLEE27 pKa = 5.17 DD28 pKa = 3.54 DD29 pKa = 4.44 TKK31 pKa = 11.23 SEE33 pKa = 4.05 PSEE36 pKa = 4.32 CGSPTEE42 pKa = 4.25 RR43 pKa = 11.84 GIPSYY48 pKa = 11.14 YY49 pKa = 10.23 LAEE52 pKa = 4.33 EE53 pKa = 4.11 LDD55 pKa = 3.65 EE56 pKa = 5.41 CEE58 pKa = 4.8 EE59 pKa = 4.18 EE60 pKa = 5.66 DD61 pKa = 5.62 SEE63 pKa = 5.45 EE64 pKa = 5.49 DD65 pKa = 4.35 DD66 pKa = 5.66 DD67 pKa = 5.32 NLPTEE72 pKa = 4.64 IPDD75 pKa = 3.9 PPTVDD80 pKa = 2.93 MLEE83 pKa = 5.17 AIMEE87 pKa = 4.2 DD88 pKa = 4.12 EE89 pKa = 4.9 IDD91 pKa = 3.48 DD92 pKa = 3.59 TAYY95 pKa = 10.27 QVHH98 pKa = 6.5 FEE100 pKa = 5.15 AKK102 pKa = 8.45 QTWKK106 pKa = 10.32 PVIEE110 pKa = 4.24 TGGNEE115 pKa = 3.69 RR116 pKa = 11.84 GKK118 pKa = 9.53 FTLSVPQNLSALQLLQWEE136 pKa = 4.59 TGIHH140 pKa = 6.17 ALAEE144 pKa = 4.11 RR145 pKa = 11.84 LGGCRR150 pKa = 11.84 LLQISTRR157 pKa = 11.84 GTRR160 pKa = 11.84 DD161 pKa = 3.28 GIEE164 pKa = 3.92 FTVRR168 pKa = 11.84 EE169 pKa = 4.52 TPCVSPASDD178 pKa = 5.56 PIPSTSRR185 pKa = 11.84 SSSIASNVSTRR196 pKa = 11.84 QTEE199 pKa = 4.55 SPGSKK204 pKa = 10.32 SNTSLGIPEE213 pKa = 4.86 APANLIDD220 pKa = 4.02 MGAIDD225 pKa = 5.6 KK226 pKa = 10.4 EE227 pKa = 4.53 FILAAISPSDD237 pKa = 3.73 PPYY240 pKa = 11.32 KK241 pKa = 9.77 NTLRR245 pKa = 11.84 NLFGSGDD252 pKa = 3.47 SFEE255 pKa = 5.11 QYY257 pKa = 10.47 NQTGIYY263 pKa = 9.01 SLKK266 pKa = 9.69 EE267 pKa = 3.52 LVIAGLKK274 pKa = 10.27 RR275 pKa = 11.84 KK276 pKa = 9.84 GIYY279 pKa = 9.28 NRR281 pKa = 11.84 IRR283 pKa = 11.84 IRR285 pKa = 11.84 CHH287 pKa = 7.41 LEE289 pKa = 3.46 PQFNN293 pKa = 3.59
Molecular weight: 32.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.42
IPC2_protein 4.355
IPC_protein 4.304
Toseland 4.139
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.113
Rodwell 4.139
Grimsley 4.05
Solomon 4.24
Lehninger 4.202
Nozaki 4.355
DTASelect 4.495
Thurlkill 4.139
EMBOSS 4.126
Sillero 4.406
Patrickios 3.706
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.334
Protein with the highest isoelectric point:
>tr|Q9WH77|Q9WH77_CHAV Isoform of P13180 Glycoprotein G (Fragment) OS=Chandipura virus (strain I653514) OX=11273 GN=G PE=4 SV=1
GG1 pKa = 7.42 LKK3 pKa = 10.34 RR4 pKa = 11.84 KK5 pKa = 9.87 GIYY8 pKa = 9.28 NRR10 pKa = 11.84 IRR12 pKa = 11.84 IRR14 pKa = 11.84 CHH16 pKa = 7.41 LEE18 pKa = 3.46 PQFNN22 pKa = 3.59
Molecular weight: 2.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.926
IPC_protein 10.965
Toseland 11.067
ProMoST 11.096
Dawson 11.125
Bjellqvist 10.935
Wikipedia 11.433
Rodwell 11.228
Grimsley 11.169
Solomon 11.389
Lehninger 11.345
Nozaki 11.067
DTASelect 10.935
Thurlkill 11.067
EMBOSS 11.506
Sillero 11.082
Patrickios 11.082
IPC_peptide 11.403
IPC2_peptide 10.262
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
2
7
3637
22
2092
519.6
58.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.839 ± 0.514
1.677 ± 0.307
5.884 ± 0.373
5.884 ± 0.813
4.372 ± 0.364
5.966 ± 0.451
2.42 ± 0.33
6.736 ± 0.511
6.956 ± 0.493
9.733 ± 0.699
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.557 ± 0.343
4.344 ± 0.421
4.922 ± 0.664
2.969 ± 0.317
5.197 ± 0.495
8.853 ± 0.593
5.911 ± 0.613
5.527 ± 0.729
1.925 ± 0.272
3.327 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here