Microbacterium azadirachtae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3744 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0KFV0|A0A0F0KFV0_9MICO AI-2 transport protein TqsA OS=Microbacterium azadirachtae OX=582680 GN=tqsA PE=3 SV=1
MM1 pKa = 6.67TAQRR5 pKa = 11.84ACAVSDD11 pKa = 3.88LQEE14 pKa = 4.35DD15 pKa = 4.29TPLRR19 pKa = 11.84VEE21 pKa = 4.19LDD23 pKa = 3.97GVPMAVVLDD32 pKa = 4.11SEE34 pKa = 4.99GEE36 pKa = 3.74IHH38 pKa = 7.74AIGDD42 pKa = 3.87TCTHH46 pKa = 6.37GDD48 pKa = 3.07ISLSEE53 pKa = 4.23GFVEE57 pKa = 6.4GKK59 pKa = 7.87TLEE62 pKa = 4.39CWAHH66 pKa = 6.09GSAFSLCSGKK76 pKa = 9.5PLNLPAYY83 pKa = 8.78EE84 pKa = 4.17PVPVYY89 pKa = 10.05TVVIDD94 pKa = 4.25GDD96 pKa = 4.08DD97 pKa = 3.65VMIDD101 pKa = 3.29PSVTKK106 pKa = 10.48DD107 pKa = 3.24VSALL111 pKa = 3.52

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0L060|A0A0F0L060_9MICO Acetyltransferase (GNAT) family protein OS=Microbacterium azadirachtae OX=582680 GN=RL72_01011 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84GVPAAMSMATVPSVRR17 pKa = 11.84SVHH20 pKa = 5.16VRR22 pKa = 11.84MAIAGSAVMTIVRR35 pKa = 11.84VVTATGRR42 pKa = 11.84SAAMTIVLAVTVIVRR57 pKa = 11.84SVAPASVPIVRR68 pKa = 11.84VVTVIVRR75 pKa = 11.84SAVTMTVRR83 pKa = 11.84AVRR86 pKa = 11.84GTARR90 pKa = 11.84SVVPASVPIVRR101 pKa = 11.84VVTATGRR108 pKa = 11.84SAATMTVRR116 pKa = 11.84AVRR119 pKa = 11.84GTARR123 pKa = 11.84SVVPASVPIVRR134 pKa = 11.84VVTATGRR141 pKa = 11.84SAAMTIVLAVTVIVRR156 pKa = 11.84SVVPASSPTVPAATVIVRR174 pKa = 11.84SAAPASVPIVRR185 pKa = 11.84VVTATGRR192 pKa = 11.84SAAMMTVPAGTGIARR207 pKa = 11.84SVAMTTVPAGMEE219 pKa = 4.06TGRR222 pKa = 11.84SAVASGPTVRR232 pKa = 11.84VVTGTGRR239 pKa = 11.84SAAMTTVLAGMEE251 pKa = 4.24TGRR254 pKa = 11.84SAVASVPIVRR264 pKa = 11.84VATPTRR270 pKa = 11.84ATVRR274 pKa = 11.84VTPNFPRR281 pKa = 11.84RR282 pKa = 11.84SRR284 pKa = 11.84HH285 pKa = 4.77VICTRR290 pKa = 11.84RR291 pKa = 11.84PVMSS295 pKa = 4.0

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3744

0

3744

1217104

29

2042

325.1

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.039 ± 0.055

0.489 ± 0.01

6.075 ± 0.037

5.406 ± 0.043

3.084 ± 0.025

9.097 ± 0.041

2.053 ± 0.02

4.57 ± 0.027

1.898 ± 0.032

10.239 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.77 ± 0.017

1.845 ± 0.023

5.545 ± 0.033

2.767 ± 0.022

7.543 ± 0.051

5.428 ± 0.029

5.955 ± 0.037

8.748 ± 0.037

1.533 ± 0.017

1.916 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski