Amino acid dipepetide frequency for Microbacterium azadirachtae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.996AlaAla: 21.996 ± 0.206
0.663AlaCys: 0.663 ± 0.025
8.669AlaAsp: 8.669 ± 0.093
8.664AlaGlu: 8.664 ± 0.108
4.146AlaPhe: 4.146 ± 0.063
12.656AlaGly: 12.656 ± 0.125
2.806AlaHis: 2.806 ± 0.045
5.993AlaIle: 5.993 ± 0.078
2.672AlaLys: 2.672 ± 0.052
15.24AlaLeu: 15.24 ± 0.141
2.774AlaMet: 2.774 ± 0.049
2.226AlaAsn: 2.226 ± 0.047
7.202AlaPro: 7.202 ± 0.102
4.2AlaGln: 4.2 ± 0.072
9.598AlaArg: 9.598 ± 0.121
7.063AlaSer: 7.063 ± 0.089
7.377AlaThr: 7.377 ± 0.092
11.981AlaVal: 11.981 ± 0.115
2.015AlaTrp: 2.015 ± 0.044
2.445AlaTyr: 2.445 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.723CysAla: 0.723 ± 0.024
0.052CysCys: 0.052 ± 0.007
0.269CysAsp: 0.269 ± 0.014
0.244CysGlu: 0.244 ± 0.015
0.185CysPhe: 0.185 ± 0.012
0.561CysGly: 0.561 ± 0.019
0.107CysHis: 0.107 ± 0.01
0.159CysIle: 0.159 ± 0.013
0.066CysLys: 0.066 ± 0.007
0.436CysLeu: 0.436 ± 0.02
0.081CysMet: 0.081 ± 0.01
0.098CysAsn: 0.098 ± 0.008
0.271CysPro: 0.271 ± 0.014
0.101CysGln: 0.101 ± 0.01
0.302CysArg: 0.302 ± 0.018
0.317CysSer: 0.317 ± 0.019
0.343CysThr: 0.343 ± 0.02
0.388CysVal: 0.388 ± 0.019
0.083CysTrp: 0.083 ± 0.008
0.104CysTyr: 0.104 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
9.326AspAla: 9.326 ± 0.11
0.222AspCys: 0.222 ± 0.013
4.01AspAsp: 4.01 ± 0.067
4.203AspGlu: 4.203 ± 0.066
1.595AspPhe: 1.595 ± 0.045
6.324AspGly: 6.324 ± 0.089
1.158AspHis: 1.158 ± 0.034
2.17AspIle: 2.17 ± 0.044
0.993AspLys: 0.993 ± 0.031
6.02AspLeu: 6.02 ± 0.069
0.782AspMet: 0.782 ± 0.027
0.845AspAsn: 0.845 ± 0.027
4.696AspPro: 4.696 ± 0.065
1.475AspGln: 1.475 ± 0.035
4.611AspArg: 4.611 ± 0.075
2.235AspSer: 2.235 ± 0.04
2.665AspThr: 2.665 ± 0.051
5.295AspVal: 5.295 ± 0.068
0.933AspTrp: 0.933 ± 0.027
1.186AspTyr: 1.186 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.974GluAla: 6.974 ± 0.1
0.222GluCys: 0.222 ± 0.015
2.867GluAsp: 2.867 ± 0.055
2.941GluGlu: 2.941 ± 0.052
1.579GluPhe: 1.579 ± 0.038
4.08GluGly: 4.08 ± 0.063
1.482GluHis: 1.482 ± 0.038
2.789GluIle: 2.789 ± 0.047
1.276GluLys: 1.276 ± 0.037
6.208GluLeu: 6.208 ± 0.095
0.849GluMet: 0.849 ± 0.029
1.126GluAsn: 1.126 ± 0.031
2.793GluPro: 2.793 ± 0.057
2.061GluGln: 2.061 ± 0.044
5.243GluArg: 5.243 ± 0.087
2.5GluSer: 2.5 ± 0.052
2.942GluThr: 2.942 ± 0.048
4.263GluVal: 4.263 ± 0.063
0.805GluTrp: 0.805 ± 0.025
1.059GluTyr: 1.059 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.449PheAla: 4.449 ± 0.076
0.169PheCys: 0.169 ± 0.012
2.31PheAsp: 2.31 ± 0.049
1.551PheGlu: 1.551 ± 0.043
1.098PhePhe: 1.098 ± 0.037
3.46PheGly: 3.46 ± 0.064
0.564PheHis: 0.564 ± 0.023
1.026PheIle: 1.026 ± 0.034
0.386PheLys: 0.386 ± 0.021
3.0PheLeu: 3.0 ± 0.053
0.395PheMet: 0.395 ± 0.017
0.598PheAsn: 0.598 ± 0.025
1.453PhePro: 1.453 ± 0.039
0.776PheGln: 0.776 ± 0.025
1.866PheArg: 1.866 ± 0.04
1.731PheSer: 1.731 ± 0.039
2.134PheThr: 2.134 ± 0.049
2.762PheVal: 2.762 ± 0.053
0.514PheTrp: 0.514 ± 0.021
0.598PheTyr: 0.598 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
11.553GlyAla: 11.553 ± 0.114
0.539GlyCys: 0.539 ± 0.021
4.985GlyAsp: 4.985 ± 0.075
4.668GlyGlu: 4.668 ± 0.063
3.32GlyPhe: 3.32 ± 0.05
7.797GlyGly: 7.797 ± 0.112
1.787GlyHis: 1.787 ± 0.037
4.919GlyIle: 4.919 ± 0.07
2.095GlyLys: 2.095 ± 0.046
8.987GlyLeu: 8.987 ± 0.113
1.99GlyMet: 1.99 ± 0.043
1.607GlyAsn: 1.607 ± 0.047
3.886GlyPro: 3.886 ± 0.055
2.356GlyGln: 2.356 ± 0.042
6.7GlyArg: 6.7 ± 0.087
5.94GlySer: 5.94 ± 0.075
5.688GlyThr: 5.688 ± 0.078
8.066GlyVal: 8.066 ± 0.095
1.801GlyTrp: 1.801 ± 0.041
2.28GlyTyr: 2.28 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.725HisAla: 2.725 ± 0.054
0.103HisCys: 0.103 ± 0.009
1.403HisAsp: 1.403 ± 0.035
1.177HisGlu: 1.177 ± 0.033
0.547HisPhe: 0.547 ± 0.02
2.223HisGly: 2.223 ± 0.05
0.569HisHis: 0.569 ± 0.025
0.696HisIle: 0.696 ± 0.026
0.282HisLys: 0.282 ± 0.013
2.038HisLeu: 2.038 ± 0.042
0.319HisMet: 0.319 ± 0.017
0.32HisAsn: 0.32 ± 0.016
1.559HisPro: 1.559 ± 0.036
0.516HisGln: 0.516 ± 0.022
1.746HisArg: 1.746 ± 0.039
0.917HisSer: 0.917 ± 0.025
0.979HisThr: 0.979 ± 0.03
1.717HisVal: 1.717 ± 0.035
0.313HisTrp: 0.313 ± 0.015
0.385HisTyr: 0.385 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.565IleAla: 7.565 ± 0.09
0.233IleCys: 0.233 ± 0.015
3.3IleAsp: 3.3 ± 0.045
2.591IleGlu: 2.591 ± 0.044
1.05IlePhe: 1.05 ± 0.037
4.887IleGly: 4.887 ± 0.078
0.707IleHis: 0.707 ± 0.024
1.638IleIle: 1.638 ± 0.041
0.677IleLys: 0.677 ± 0.026
4.024IleLeu: 4.024 ± 0.068
0.624IleMet: 0.624 ± 0.022
0.819IleAsn: 0.819 ± 0.028
2.584IlePro: 2.584 ± 0.046
0.965IleGln: 0.965 ± 0.031
3.075IleArg: 3.075 ± 0.055
2.223IleSer: 2.223 ± 0.045
2.724IleThr: 2.724 ± 0.047
4.774IleVal: 4.774 ± 0.078
0.541IleTrp: 0.541 ± 0.022
0.699IleTyr: 0.699 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
2.538LysAla: 2.538 ± 0.055
0.061LysCys: 0.061 ± 0.008
1.222LysAsp: 1.222 ± 0.037
0.887LysGlu: 0.887 ± 0.03
0.427LysPhe: 0.427 ± 0.019
1.568LysGly: 1.568 ± 0.04
0.45LysHis: 0.45 ± 0.019
0.886LysIle: 0.886 ± 0.033
0.721LysLys: 0.721 ± 0.032
1.715LysLeu: 1.715 ± 0.041
0.347LysMet: 0.347 ± 0.017
0.537LysAsn: 0.537 ± 0.026
1.114LysPro: 1.114 ± 0.036
0.658LysGln: 0.658 ± 0.023
1.328LysArg: 1.328 ± 0.036
1.062LysSer: 1.062 ± 0.035
1.273LysThr: 1.273 ± 0.038
1.55LysVal: 1.55 ± 0.043
0.219LysTrp: 0.219 ± 0.014
0.414LysTyr: 0.414 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
14.782LeuAla: 14.782 ± 0.128
0.577LeuCys: 0.577 ± 0.024
6.558LeuAsp: 6.558 ± 0.085
4.811LeuGlu: 4.811 ± 0.079
2.915LeuPhe: 2.915 ± 0.055
9.404LeuGly: 9.404 ± 0.093
2.083LeuHis: 2.083 ± 0.04
4.69LeuIle: 4.69 ± 0.073
1.67LeuLys: 1.67 ± 0.045
10.708LeuLeu: 10.708 ± 0.147
1.598LeuMet: 1.598 ± 0.037
1.746LeuAsn: 1.746 ± 0.044
5.811LeuPro: 5.811 ± 0.102
2.665LeuGln: 2.665 ± 0.05
8.09LeuArg: 8.09 ± 0.096
5.835LeuSer: 5.835 ± 0.072
6.213LeuThr: 6.213 ± 0.076
9.178LeuVal: 9.178 ± 0.122
1.354LeuTrp: 1.354 ± 0.04
1.702LeuTyr: 1.702 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.071MetAla: 2.071 ± 0.041
0.087MetCys: 0.087 ± 0.009
0.824MetAsp: 0.824 ± 0.024
0.616MetGlu: 0.616 ± 0.024
0.505MetPhe: 0.505 ± 0.02
1.249MetGly: 1.249 ± 0.037
0.353MetHis: 0.353 ± 0.017
0.886MetIle: 0.886 ± 0.03
0.393MetLys: 0.393 ± 0.019
1.874MetLeu: 1.874 ± 0.044
0.351MetMet: 0.351 ± 0.021
0.483MetAsn: 0.483 ± 0.018
1.142MetPro: 1.142 ± 0.028
0.504MetGln: 0.504 ± 0.022
1.378MetArg: 1.378 ± 0.035
1.491MetSer: 1.491 ± 0.033
1.786MetThr: 1.786 ± 0.037
1.25MetVal: 1.25 ± 0.034
0.214MetTrp: 0.214 ± 0.014
0.246MetTyr: 0.246 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.458AsnAla: 2.458 ± 0.05
0.084AsnCys: 0.084 ± 0.009
1.028AsnAsp: 1.028 ± 0.032
0.836AsnGlu: 0.836 ± 0.028
0.56AsnPhe: 0.56 ± 0.024
1.926AsnGly: 1.926 ± 0.048
0.366AsnHis: 0.366 ± 0.018
0.774AsnIle: 0.774 ± 0.025
0.376AsnLys: 0.376 ± 0.021
1.817AsnLeu: 1.817 ± 0.046
0.305AsnMet: 0.305 ± 0.017
0.432AsnAsn: 0.432 ± 0.023
1.457AsnPro: 1.457 ± 0.04
0.495AsnGln: 0.495 ± 0.024
1.202AsnArg: 1.202 ± 0.034
0.925AsnSer: 0.925 ± 0.033
1.132AsnThr: 1.132 ± 0.031
1.527AsnVal: 1.527 ± 0.04
0.311AsnTrp: 0.311 ± 0.015
0.444AsnTyr: 0.444 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
7.877ProAla: 7.877 ± 0.094
0.182ProCys: 0.182 ± 0.013
3.87ProAsp: 3.87 ± 0.057
3.814ProGlu: 3.814 ± 0.065
1.75ProPhe: 1.75 ± 0.043
5.326ProGly: 5.326 ± 0.111
1.24ProHis: 1.24 ± 0.033
2.152ProIle: 2.152 ± 0.046
1.019ProLys: 1.019 ± 0.032
5.216ProLeu: 5.216 ± 0.067
0.876ProMet: 0.876 ± 0.026
1.026ProAsn: 1.026 ± 0.033
2.326ProPro: 2.326 ± 0.058
1.628ProGln: 1.628 ± 0.042
3.672ProArg: 3.672 ± 0.068
3.304ProSer: 3.304 ± 0.056
3.202ProThr: 3.202 ± 0.056
5.05ProVal: 5.05 ± 0.068
0.9ProTrp: 0.9 ± 0.026
1.021ProTyr: 1.021 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.662GlnAla: 3.662 ± 0.057
0.116GlnCys: 0.116 ± 0.01
1.385GlnAsp: 1.385 ± 0.029
1.315GlnGlu: 1.315 ± 0.036
0.806GlnPhe: 0.806 ± 0.027
2.235GlnGly: 2.235 ± 0.047
0.667GlnHis: 0.667 ± 0.026
1.403GlnIle: 1.403 ± 0.033
0.706GlnLys: 0.706 ± 0.026
3.025GlnLeu: 3.025 ± 0.056
0.467GlnMet: 0.467 ± 0.02
0.692GlnAsn: 0.692 ± 0.025
1.417GlnPro: 1.417 ± 0.039
1.158GlnGln: 1.158 ± 0.043
2.394GlnArg: 2.394 ± 0.047
1.406GlnSer: 1.406 ± 0.036
1.545GlnThr: 1.545 ± 0.039
2.22GlnVal: 2.22 ± 0.039
0.449GlnTrp: 0.449 ± 0.021
0.604GlnTyr: 0.604 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.701ArgAla: 9.701 ± 0.133
0.314ArgCys: 0.314 ± 0.02
4.304ArgAsp: 4.304 ± 0.075
4.297ArgGlu: 4.297 ± 0.074
2.448ArgPhe: 2.448 ± 0.048
5.742ArgGly: 5.742 ± 0.076
1.59ArgHis: 1.59 ± 0.041
4.32ArgIle: 4.32 ± 0.062
1.241ArgLys: 1.241 ± 0.034
7.542ArgLeu: 7.542 ± 0.097
1.858ArgMet: 1.858 ± 0.041
1.283ArgAsn: 1.283 ± 0.035
3.932ArgPro: 3.932 ± 0.071
1.969ArgGln: 1.969 ± 0.049
7.326ArgArg: 7.326 ± 0.113
4.074ArgSer: 4.074 ± 0.063
4.764ArgThr: 4.764 ± 0.073
5.94ArgVal: 5.94 ± 0.08
1.228ArgTrp: 1.228 ± 0.032
1.557ArgTyr: 1.557 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.653SerAla: 7.653 ± 0.1
0.286SerCys: 0.286 ± 0.018
3.038SerAsp: 3.038 ± 0.057
2.51SerGlu: 2.51 ± 0.049
1.903SerPhe: 1.903 ± 0.042
5.8SerGly: 5.8 ± 0.078
0.944SerHis: 0.944 ± 0.025
2.388SerIle: 2.388 ± 0.043
0.993SerLys: 0.993 ± 0.033
5.057SerLeu: 5.057 ± 0.066
1.094SerMet: 1.094 ± 0.032
0.966SerAsn: 0.966 ± 0.033
3.108SerPro: 3.108 ± 0.051
1.277SerGln: 1.277 ± 0.032
3.808SerArg: 3.808 ± 0.06
3.345SerSer: 3.345 ± 0.063
3.567SerThr: 3.567 ± 0.062
4.523SerVal: 4.523 ± 0.068
0.92SerTrp: 0.92 ± 0.026
1.099SerTyr: 1.099 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
8.349ThrAla: 8.349 ± 0.09
0.279ThrCys: 0.279 ± 0.016
3.325ThrAsp: 3.325 ± 0.057
2.877ThrGlu: 2.877 ± 0.047
1.804ThrPhe: 1.804 ± 0.042
5.905ThrGly: 5.905 ± 0.075
1.152ThrHis: 1.152 ± 0.03
2.986ThrIle: 2.986 ± 0.048
1.17ThrLys: 1.17 ± 0.039
5.907ThrLeu: 5.907 ± 0.076
0.996ThrMet: 0.996 ± 0.026
1.064ThrAsn: 1.064 ± 0.031
3.968ThrPro: 3.968 ± 0.064
1.421ThrGln: 1.421 ± 0.032
3.684ThrArg: 3.684 ± 0.06
3.231ThrSer: 3.231 ± 0.054
3.793ThrThr: 3.793 ± 0.068
5.693ThrVal: 5.693 ± 0.09
0.888ThrTrp: 0.888 ± 0.03
1.06ThrTyr: 1.06 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
11.74ValAla: 11.74 ± 0.126
0.489ValCys: 0.489 ± 0.018
5.37ValAsp: 5.37 ± 0.073
4.395ValGlu: 4.395 ± 0.057
2.934ValPhe: 2.934 ± 0.058
6.73ValGly: 6.73 ± 0.083
1.856ValHis: 1.856 ± 0.037
4.276ValIle: 4.276 ± 0.063
1.568ValLys: 1.568 ± 0.047
9.834ValLeu: 9.834 ± 0.11
1.423ValMet: 1.423 ± 0.031
1.716ValAsn: 1.716 ± 0.043
4.95ValPro: 4.95 ± 0.075
2.349ValGln: 2.349 ± 0.042
6.419ValArg: 6.419 ± 0.074
4.789ValSer: 4.789 ± 0.064
5.309ValThr: 5.309 ± 0.067
8.663ValVal: 8.663 ± 0.104
1.181ValTrp: 1.181 ± 0.038
1.494ValTyr: 1.494 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.721TrpAla: 1.721 ± 0.042
0.094TrpCys: 0.094 ± 0.008
0.785TrpAsp: 0.785 ± 0.026
0.674TrpGlu: 0.674 ± 0.025
0.6TrpPhe: 0.6 ± 0.023
1.154TrpGly: 1.154 ± 0.032
0.354TrpHis: 0.354 ± 0.018
0.866TrpIle: 0.866 ± 0.029
0.315TrpLys: 0.315 ± 0.018
1.697TrpLeu: 1.697 ± 0.043
0.336TrpMet: 0.336 ± 0.018
0.462TrpAsn: 0.462 ± 0.021
0.747TrpPro: 0.747 ± 0.027
0.566TrpGln: 0.566 ± 0.022
1.281TrpArg: 1.281 ± 0.032
0.906TrpSer: 0.906 ± 0.028
1.0TrpThr: 1.0 ± 0.029
1.121TrpVal: 1.121 ± 0.032
0.358TrpTrp: 0.358 ± 0.018
0.293TrpTyr: 0.293 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.523TyrAla: 2.523 ± 0.047
0.119TyrCys: 0.119 ± 0.009
1.226TyrAsp: 1.226 ± 0.033
0.987TyrGlu: 0.987 ± 0.028
0.667TyrPhe: 0.667 ± 0.021
1.937TyrGly: 1.937 ± 0.039
0.298TyrHis: 0.298 ± 0.016
0.683TyrIle: 0.683 ± 0.024
0.358TyrLys: 0.358 ± 0.018
2.044TyrLeu: 2.044 ± 0.044
0.241TyrMet: 0.241 ± 0.014
0.406TyrAsn: 0.406 ± 0.02
1.032TyrPro: 1.032 ± 0.034
0.531TyrGln: 0.531 ± 0.023
1.707TyrArg: 1.707 ± 0.038
0.984TyrSer: 0.984 ± 0.03
1.12TyrThr: 1.12 ± 0.036
1.524TyrVal: 1.524 ± 0.035
0.303TyrTrp: 0.303 ± 0.016
0.472TyrTyr: 0.472 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3744 proteins (1217104 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski