Salinimicrobium catena
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2965 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H5JXK7|A0A1H5JXK7_9FLAO Hemolysin contains CBS domains OS=Salinimicrobium catena OX=390640 GN=SAMN04488034_101899 PE=3 SV=1
MM1 pKa = 7.43 EE2 pKa = 5.85 NSTKK6 pKa = 10.6 HH7 pKa = 5.99 LGGAGGKK14 pKa = 8.98 LVHH17 pKa = 5.91 SVKK20 pKa = 10.83 YY21 pKa = 10.5 ILIFLLLWVVGCSDD35 pKa = 5.28 DD36 pKa = 5.11 DD37 pKa = 5.3 DD38 pKa = 4.72 NVPPPVEE45 pKa = 4.02 PTQSIDD51 pKa = 3.45 LVLVAEE57 pKa = 4.69 NMVSPIGLMPSPDD70 pKa = 3.72 DD71 pKa = 3.44 TGNLYY76 pKa = 11.04 VIDD79 pKa = 4.18 QIGKK83 pKa = 8.46 VWVIDD88 pKa = 3.83 DD89 pKa = 4.08 SGNLLEE95 pKa = 5.69 EE96 pKa = 4.69 PFLDD100 pKa = 3.78 VGPMMVTLDD109 pKa = 3.45 PGYY112 pKa = 10.42 DD113 pKa = 3.35 EE114 pKa = 6.13 RR115 pKa = 11.84 GLLGLAFHH123 pKa = 7.66 PDD125 pKa = 3.2 YY126 pKa = 11.16 SSNGRR131 pKa = 11.84 LFVYY135 pKa = 8.11 YY136 pKa = 8.3 TAPPIAGGPEE146 pKa = 3.99 EE147 pKa = 5.5 GEE149 pKa = 3.92 MWDD152 pKa = 3.28 NTSRR156 pKa = 11.84 ISEE159 pKa = 4.23 FTVSGSNPNQVQAGSEE175 pKa = 4.14 KK176 pKa = 10.32 IILDD180 pKa = 3.26 INQPQFNHH188 pKa = 6.37 EE189 pKa = 4.19 GGTIAFGPDD198 pKa = 2.8 GYY200 pKa = 11.12 LYY202 pKa = 10.35 ISIGDD207 pKa = 3.75 GGASNDD213 pKa = 3.48 VAPGHH218 pKa = 6.01 VEE220 pKa = 3.44 DD221 pKa = 4.97 WYY223 pKa = 10.86 DD224 pKa = 3.66 VNAGGNGQDD233 pKa = 3.69 VEE235 pKa = 5.01 SNLLGNILRR244 pKa = 11.84 IDD246 pKa = 3.53 VDD248 pKa = 3.51 GGDD251 pKa = 4.04 PYY253 pKa = 11.14 GIPGDD258 pKa = 3.91 NPFVDD263 pKa = 3.09 SDD265 pKa = 3.75 GRR267 pKa = 11.84 DD268 pKa = 3.14 EE269 pKa = 4.09 IYY271 pKa = 10.46 AYY273 pKa = 10.4 GFRR276 pKa = 11.84 NPFRR280 pKa = 11.84 FSFDD284 pKa = 2.67 MGGDD288 pKa = 3.15 HH289 pKa = 7.52 RR290 pKa = 11.84 LFVGDD295 pKa = 4.24 AGQNLWEE302 pKa = 4.86 EE303 pKa = 4.06 ISVVEE308 pKa = 4.61 NGGNYY313 pKa = 8.44 GWNVKK318 pKa = 9.97 EE319 pKa = 4.2 GTHH322 pKa = 6.87 CFDD325 pKa = 4.13 ASNNEE330 pKa = 4.05 NVLEE334 pKa = 4.24 SCPDD338 pKa = 3.12 VDD340 pKa = 4.16 VLGNEE345 pKa = 4.8 LIDD348 pKa = 4.09 PVIEE352 pKa = 3.92 MSNANNPAGGSTVTIIGGYY371 pKa = 7.34 VYY373 pKa = 10.37 RR374 pKa = 11.84 GTAIPEE380 pKa = 3.82 LSGQYY385 pKa = 10.84 LFGSFSQDD393 pKa = 3.14 FEE395 pKa = 4.4 PTGEE399 pKa = 4.13 VFFANPSGAGLWDD412 pKa = 3.87 FEE414 pKa = 4.19 EE415 pKa = 5.94 LEE417 pKa = 4.5 FKK419 pKa = 9.84 STSGDD424 pKa = 2.44 IGYY427 pKa = 9.38 YY428 pKa = 10.43 LKK430 pKa = 11.21 GFGQDD435 pKa = 3.37 LDD437 pKa = 4.11 GEE439 pKa = 4.52 IYY441 pKa = 10.39 IAVSSMLGPDD451 pKa = 3.52 GNTGKK456 pKa = 10.0 IFKK459 pKa = 10.24 ISLVDD464 pKa = 3.67 DD465 pKa = 4.19
Molecular weight: 50.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.579
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.923
Patrickios 1.113
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A1H5JB70|A0A1H5JB70_9FLAO Uncharacterized protein OS=Salinimicrobium catena OX=390640 GN=SAMN04488034_101685 PE=4 SV=1
MM1 pKa = 7.22 FSIGRR6 pKa = 11.84 LFCCYY11 pKa = 9.94 FGHH14 pKa = 7.23 RR15 pKa = 11.84 LHH17 pKa = 7.04 ISRR20 pKa = 11.84 RR21 pKa = 11.84 ITNHH25 pKa = 4.96 IKK27 pKa = 10.11 EE28 pKa = 4.37 YY29 pKa = 10.18 SCTRR33 pKa = 11.84 CGQEE37 pKa = 3.65 MTDD40 pKa = 3.4 TANGFLANLTPRR52 pKa = 11.84 LQEE55 pKa = 4.08 TNTFLAKK62 pKa = 9.19 IHH64 pKa = 5.2 EE65 pKa = 4.3 RR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 NRR70 pKa = 11.84 KK71 pKa = 8.52 MILKK75 pKa = 10.42 ASS77 pKa = 3.67
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.487
IPC_protein 10.072
Toseland 10.452
ProMoST 10.116
Dawson 10.57
Bjellqvist 10.306
Wikipedia 10.76
Rodwell 10.804
Grimsley 10.628
Solomon 10.672
Lehninger 10.643
Nozaki 10.511
DTASelect 10.262
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.511
Patrickios 10.599
IPC_peptide 10.672
IPC2_peptide 9.663
IPC2.peptide.svr19 8.419
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2965
0
2965
981291
39
3347
331.0
37.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.722 ± 0.045
0.653 ± 0.015
5.439 ± 0.034
7.928 ± 0.053
5.189 ± 0.036
6.653 ± 0.042
1.856 ± 0.021
7.039 ± 0.037
7.29 ± 0.054
9.607 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.323 ± 0.022
5.168 ± 0.033
3.703 ± 0.022
3.477 ± 0.022
4.152 ± 0.026
6.156 ± 0.031
5.236 ± 0.039
6.442 ± 0.033
1.089 ± 0.018
3.877 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here