Gleimia hominis
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1778 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N6UM46|A0A2N6UM46_9ACTO Endopeptidase La OS=Gleimia hominis OX=595468 GN=CJ187_08160 PE=3 SV=1
MM1 pKa = 7.6 TYY3 pKa = 10.0 IIAQPCVDD11 pKa = 3.72 VKK13 pKa = 11.25 DD14 pKa = 3.94 RR15 pKa = 11.84 ACVDD19 pKa = 3.71 EE20 pKa = 5.5 CPVDD24 pKa = 4.76 CIYY27 pKa = 11.04 EE28 pKa = 4.42 GNRR31 pKa = 11.84 SLYY34 pKa = 9.5 IHH36 pKa = 7.14 PEE38 pKa = 3.78 EE39 pKa = 5.19 CVDD42 pKa = 4.78 CGACEE47 pKa = 3.85 PVCPVEE53 pKa = 4.7 AIFYY57 pKa = 10.04 EE58 pKa = 4.74 DD59 pKa = 4.7 DD60 pKa = 5.22 VPDD63 pKa = 3.5 EE64 pKa = 3.82 WSDD67 pKa = 3.76 YY68 pKa = 10.44 YY69 pKa = 10.79 RR70 pKa = 11.84 ANVDD74 pKa = 3.84 FFKK77 pKa = 11.18 DD78 pKa = 3.3 LGSPGGAMAVGKK90 pKa = 10.2 QDD92 pKa = 4.22 FDD94 pKa = 4.87 DD95 pKa = 4.72 EE96 pKa = 4.66 MIEE99 pKa = 4.11 NLPPQNQDD107 pKa = 2.47
Molecular weight: 12.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.869
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.439
ProMoST 3.783
Dawson 3.656
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.783
Patrickios 0.057
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A2N6UNJ9|A0A2N6UNJ9_9ACTO Amidohydrolase OS=Gleimia hominis OX=595468 GN=CJ187_06315 PE=4 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.09 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 KK16 pKa = 8.57 HH17 pKa = 4.77 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSNRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.83 GRR40 pKa = 11.84 AKK42 pKa = 10.68 LSAA45 pKa = 3.92
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1778
0
1778
628026
25
2952
353.2
38.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.186 ± 0.073
0.815 ± 0.015
5.656 ± 0.051
5.901 ± 0.057
3.251 ± 0.038
8.097 ± 0.051
2.171 ± 0.023
4.865 ± 0.048
3.758 ± 0.052
9.549 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.283 ± 0.025
2.989 ± 0.032
4.954 ± 0.039
3.985 ± 0.04
6.341 ± 0.059
5.844 ± 0.038
6.078 ± 0.042
8.434 ± 0.046
1.466 ± 0.025
2.376 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here