Salmonella phage TS6
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5S9BG12|A0A5S9BG12_9CAUD Uncharacterized protein OS=Salmonella phage TS6 OX=2491323 GN=AP6_009 PE=4 SV=1
MM1 pKa = 7.26 AQYY4 pKa = 9.0 EE5 pKa = 4.41 IPDD8 pKa = 4.16 CNDD11 pKa = 3.99 PEE13 pKa = 4.49 WQSQMLRR20 pKa = 11.84 EE21 pKa = 4.22 LEE23 pKa = 4.39 EE24 pKa = 4.01 NLQLLRR30 pKa = 11.84 DD31 pKa = 5.06 DD32 pKa = 5.39 IGDD35 pKa = 4.28 DD36 pKa = 3.74 NPNLVDD42 pKa = 3.67 SLSIVDD48 pKa = 4.87 ALRR51 pKa = 11.84 QYY53 pKa = 11.13 SGYY56 pKa = 11.21
Molecular weight: 6.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 3.63
IPC_protein 3.554
Toseland 3.35
ProMoST 3.757
Dawson 3.567
Bjellqvist 3.732
Wikipedia 3.541
Rodwell 3.389
Grimsley 3.274
Solomon 3.516
Lehninger 3.478
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.541
Sillero 3.681
Patrickios 1.825
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|A0A5S9H9K4|A0A5S9H9K4_9CAUD Uncharacterized protein OS=Salmonella phage TS6 OX=2491323 GN=AP6_025 PE=4 SV=1
MM1 pKa = 7.52 SLATDD6 pKa = 3.38 ILKK9 pKa = 10.37 RR10 pKa = 11.84 ANSDD14 pKa = 3.34 LSPPSKK20 pKa = 10.44 SIRR23 pKa = 11.84 ADD25 pKa = 3.31 YY26 pKa = 9.1 MGPAIRR32 pKa = 11.84 KK33 pKa = 6.74 KK34 pKa = 10.59 AKK36 pKa = 9.47 RR37 pKa = 11.84 AAPKK41 pKa = 10.45 GPRR44 pKa = 11.84 LNRR47 pKa = 11.84 KK48 pKa = 8.83 KK49 pKa = 10.79 GKK51 pKa = 6.76 TVNYY55 pKa = 8.1 VAPKK59 pKa = 8.35 HH60 pKa = 4.95 EE61 pKa = 4.5 HH62 pKa = 4.99 VAGVAFNSRR71 pKa = 11.84 NNTWDD76 pKa = 3.03 AYY78 pKa = 10.53 FYY80 pKa = 11.24 DD81 pKa = 4.9 GIKK84 pKa = 9.55 TIRR87 pKa = 11.84 IGMFHH92 pKa = 5.71 TQARR96 pKa = 11.84 ALIARR101 pKa = 11.84 RR102 pKa = 11.84 IYY104 pKa = 9.73 MYY106 pKa = 9.49 WRR108 pKa = 11.84 KK109 pKa = 10.11 CGFDD113 pKa = 3.89 NIPNKK118 pKa = 9.56 PEE120 pKa = 3.47 RR121 pKa = 11.84 RR122 pKa = 11.84 LYY124 pKa = 10.15 TMRR127 pKa = 11.84 NYY129 pKa = 10.69 SDD131 pKa = 3.46 KK132 pKa = 11.39 SS133 pKa = 3.41
Molecular weight: 15.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 10.014
IPC_protein 10.701
Toseland 10.789
ProMoST 10.452
Dawson 10.891
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.257
Grimsley 10.95
Solomon 10.979
Lehninger 10.95
Nozaki 10.76
DTASelect 10.584
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.818
Patrickios 10.965
IPC_peptide 10.994
IPC2_peptide 9.414
IPC2.peptide.svr19 8.349
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
12657
39
852
194.7
21.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.105 ± 0.461
1.146 ± 0.134
6.384 ± 0.276
6.415 ± 0.333
3.437 ± 0.18
7.632 ± 0.377
1.691 ± 0.181
5.143 ± 0.192
5.862 ± 0.306
7.806 ± 0.308
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.457 ± 0.152
4.756 ± 0.254
3.903 ± 0.21
4.18 ± 0.322
5.333 ± 0.235
5.87 ± 0.303
6.17 ± 0.318
6.992 ± 0.315
1.406 ± 0.143
3.31 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here