Amino acid dipepetide frequency for Salmonella phage TS6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.904AlaAla: 10.904 ± 1.255
0.948AlaCys: 0.948 ± 0.289
6.874AlaAsp: 6.874 ± 0.785
6.716AlaGlu: 6.716 ± 0.827
3.793AlaPhe: 3.793 ± 0.55
8.534AlaGly: 8.534 ± 0.778
1.896AlaHis: 1.896 ± 0.43
5.768AlaIle: 5.768 ± 0.811
5.452AlaLys: 5.452 ± 0.806
8.613AlaLeu: 8.613 ± 0.904
2.054AlaMet: 2.054 ± 0.424
3.556AlaAsn: 3.556 ± 0.713
3.161AlaPro: 3.161 ± 0.549
3.714AlaGln: 3.714 ± 0.769
5.136AlaArg: 5.136 ± 0.647
6.321AlaSer: 6.321 ± 0.62
6.084AlaThr: 6.084 ± 0.706
6.874AlaVal: 6.874 ± 0.864
1.501AlaTrp: 1.501 ± 0.3
3.161AlaTyr: 3.161 ± 0.544
0.0AlaXaa: 0.0 ± 0.0
Cys
1.343CysAla: 1.343 ± 0.357
0.237CysCys: 0.237 ± 0.141
0.474CysAsp: 0.474 ± 0.216
0.869CysGlu: 0.869 ± 0.37
0.079CysPhe: 0.079 ± 0.078
1.422CysGly: 1.422 ± 0.42
0.395CysHis: 0.395 ± 0.161
0.237CysIle: 0.237 ± 0.11
0.869CysLys: 0.869 ± 0.244
1.027CysLeu: 1.027 ± 0.392
0.158CysMet: 0.158 ± 0.111
0.632CysAsn: 0.632 ± 0.245
0.474CysPro: 0.474 ± 0.182
0.553CysGln: 0.553 ± 0.28
0.79CysArg: 0.79 ± 0.278
0.316CysSer: 0.316 ± 0.158
0.474CysThr: 0.474 ± 0.195
0.474CysVal: 0.474 ± 0.179
0.237CysTrp: 0.237 ± 0.131
0.395CysTyr: 0.395 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
6.558AspAla: 6.558 ± 0.713
0.632AspCys: 0.632 ± 0.302
4.504AspAsp: 4.504 ± 1.009
5.373AspGlu: 5.373 ± 1.103
2.449AspPhe: 2.449 ± 0.434
5.294AspGly: 5.294 ± 0.76
0.632AspHis: 0.632 ± 0.196
3.477AspIle: 3.477 ± 0.487
3.556AspLys: 3.556 ± 0.547
5.768AspLeu: 5.768 ± 0.851
1.501AspMet: 1.501 ± 0.282
2.686AspAsn: 2.686 ± 0.517
2.212AspPro: 2.212 ± 0.404
1.58AspGln: 1.58 ± 0.357
2.449AspArg: 2.449 ± 0.505
4.346AspSer: 4.346 ± 0.653
3.556AspThr: 3.556 ± 0.606
4.346AspVal: 4.346 ± 0.685
0.869AspTrp: 0.869 ± 0.248
2.054AspTyr: 2.054 ± 0.388
0.0AspXaa: 0.0 ± 0.0
Glu
6.4GluAla: 6.4 ± 0.92
0.632GluCys: 0.632 ± 0.316
4.583GluAsp: 4.583 ± 0.579
4.188GluGlu: 4.188 ± 0.793
2.449GluPhe: 2.449 ± 0.451
4.188GluGly: 4.188 ± 0.505
1.343GluHis: 1.343 ± 0.345
3.319GluIle: 3.319 ± 0.392
3.951GluLys: 3.951 ± 0.549
6.163GluLeu: 6.163 ± 0.911
2.528GluMet: 2.528 ± 0.465
2.607GluAsn: 2.607 ± 0.382
1.975GluPro: 1.975 ± 0.485
2.765GluGln: 2.765 ± 0.53
3.082GluArg: 3.082 ± 0.549
2.924GluSer: 2.924 ± 0.439
3.635GluThr: 3.635 ± 0.484
4.109GluVal: 4.109 ± 0.474
1.185GluTrp: 1.185 ± 0.26
2.133GluTyr: 2.133 ± 0.353
0.0GluXaa: 0.0 ± 0.0
Phe
2.291PheAla: 2.291 ± 0.434
0.553PheCys: 0.553 ± 0.208
2.37PheAsp: 2.37 ± 0.546
2.528PheGlu: 2.528 ± 0.378
0.711PhePhe: 0.711 ± 0.243
3.003PheGly: 3.003 ± 0.489
0.711PheHis: 0.711 ± 0.263
2.291PheIle: 2.291 ± 0.383
1.501PheLys: 1.501 ± 0.348
2.212PheLeu: 2.212 ± 0.497
0.553PheMet: 0.553 ± 0.255
2.212PheAsn: 2.212 ± 0.482
1.343PhePro: 1.343 ± 0.42
0.711PheGln: 0.711 ± 0.223
2.133PheArg: 2.133 ± 0.346
2.37PheSer: 2.37 ± 0.579
2.765PheThr: 2.765 ± 0.569
2.133PheVal: 2.133 ± 0.336
0.948PheTrp: 0.948 ± 0.298
1.027PheTyr: 1.027 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
6.953GlyAla: 6.953 ± 0.725
1.106GlyCys: 1.106 ± 0.315
4.267GlyAsp: 4.267 ± 0.522
4.346GlyGlu: 4.346 ± 0.532
2.449GlyPhe: 2.449 ± 0.394
6.321GlyGly: 6.321 ± 0.875
1.027GlyHis: 1.027 ± 0.28
3.477GlyIle: 3.477 ± 0.604
4.662GlyLys: 4.662 ± 0.643
5.215GlyLeu: 5.215 ± 0.653
2.054GlyMet: 2.054 ± 0.345
4.662GlyAsn: 4.662 ± 0.551
1.975GlyPro: 1.975 ± 0.365
3.082GlyGln: 3.082 ± 0.46
4.03GlyArg: 4.03 ± 0.623
5.768GlySer: 5.768 ± 0.755
4.267GlyThr: 4.267 ± 0.693
6.321GlyVal: 6.321 ± 0.828
1.343GlyTrp: 1.343 ± 0.373
3.003GlyTyr: 3.003 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
1.343HisAla: 1.343 ± 0.356
0.395HisCys: 0.395 ± 0.145
1.027HisAsp: 1.027 ± 0.262
0.553HisGlu: 0.553 ± 0.199
0.553HisPhe: 0.553 ± 0.221
0.632HisGly: 0.632 ± 0.244
0.553HisHis: 0.553 ± 0.166
0.711HisIle: 0.711 ± 0.242
1.343HisLys: 1.343 ± 0.343
1.106HisLeu: 1.106 ± 0.31
0.395HisMet: 0.395 ± 0.195
0.869HisAsn: 0.869 ± 0.221
1.264HisPro: 1.264 ± 0.295
1.185HisGln: 1.185 ± 0.305
0.948HisArg: 0.948 ± 0.338
1.185HisSer: 1.185 ± 0.27
0.553HisThr: 0.553 ± 0.19
1.738HisVal: 1.738 ± 0.437
0.158HisTrp: 0.158 ± 0.122
0.395HisTyr: 0.395 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.136IleAla: 5.136 ± 0.809
0.711IleCys: 0.711 ± 0.193
3.319IleAsp: 3.319 ± 0.583
3.477IleGlu: 3.477 ± 0.402
1.343IlePhe: 1.343 ± 0.349
4.109IleGly: 4.109 ± 0.549
0.395IleHis: 0.395 ± 0.196
2.607IleIle: 2.607 ± 0.483
2.845IleLys: 2.845 ± 0.621
3.24IleLeu: 3.24 ± 0.55
0.948IleMet: 0.948 ± 0.215
2.528IleAsn: 2.528 ± 0.505
2.924IlePro: 2.924 ± 0.453
1.896IleGln: 1.896 ± 0.383
2.449IleArg: 2.449 ± 0.384
3.319IleSer: 3.319 ± 0.489
4.425IleThr: 4.425 ± 0.539
3.477IleVal: 3.477 ± 0.42
0.79IleTrp: 0.79 ± 0.296
1.422IleTyr: 1.422 ± 0.348
0.0IleXaa: 0.0 ± 0.0
Lys
6.953LysAla: 6.953 ± 0.863
1.106LysCys: 1.106 ± 0.312
2.607LysAsp: 2.607 ± 0.572
3.635LysGlu: 3.635 ± 0.558
2.212LysPhe: 2.212 ± 0.337
3.793LysGly: 3.793 ± 0.646
1.027LysHis: 1.027 ± 0.345
2.528LysIle: 2.528 ± 0.432
3.24LysLys: 3.24 ± 0.432
5.452LysLeu: 5.452 ± 0.871
1.896LysMet: 1.896 ± 0.513
2.528LysAsn: 2.528 ± 0.633
2.924LysPro: 2.924 ± 0.533
2.607LysGln: 2.607 ± 0.484
3.556LysArg: 3.556 ± 0.498
2.924LysSer: 2.924 ± 0.499
3.793LysThr: 3.793 ± 0.541
3.003LysVal: 3.003 ± 0.416
0.632LysTrp: 0.632 ± 0.196
2.212LysTyr: 2.212 ± 0.365
0.0LysXaa: 0.0 ± 0.0
Leu
8.217LeuAla: 8.217 ± 0.905
0.711LeuCys: 0.711 ± 0.251
4.899LeuAsp: 4.899 ± 0.55
5.294LeuGlu: 5.294 ± 0.682
2.054LeuPhe: 2.054 ± 0.433
4.82LeuGly: 4.82 ± 0.52
1.027LeuHis: 1.027 ± 0.338
3.556LeuIle: 3.556 ± 0.541
5.847LeuLys: 5.847 ± 0.679
6.163LeuLeu: 6.163 ± 0.752
1.738LeuMet: 1.738 ± 0.36
4.109LeuAsn: 4.109 ± 0.523
3.556LeuPro: 3.556 ± 0.631
3.556LeuGln: 3.556 ± 0.51
4.82LeuArg: 4.82 ± 0.688
4.82LeuSer: 4.82 ± 0.58
4.109LeuThr: 4.109 ± 0.589
5.926LeuVal: 5.926 ± 0.639
0.79LeuTrp: 0.79 ± 0.21
2.054LeuTyr: 2.054 ± 0.36
0.0LeuXaa: 0.0 ± 0.0
Met
3.161MetAla: 3.161 ± 0.459
0.553MetCys: 0.553 ± 0.205
1.422MetAsp: 1.422 ± 0.425
1.422MetGlu: 1.422 ± 0.335
0.948MetPhe: 0.948 ± 0.283
1.896MetGly: 1.896 ± 0.375
0.632MetHis: 0.632 ± 0.199
1.027MetIle: 1.027 ± 0.237
1.264MetLys: 1.264 ± 0.289
1.738MetLeu: 1.738 ± 0.364
0.632MetMet: 0.632 ± 0.265
0.79MetAsn: 0.79 ± 0.253
0.711MetPro: 0.711 ± 0.223
0.948MetGln: 0.948 ± 0.252
1.817MetArg: 1.817 ± 0.401
1.343MetSer: 1.343 ± 0.365
2.054MetThr: 2.054 ± 0.45
1.422MetVal: 1.422 ± 0.395
0.079MetTrp: 0.079 ± 0.08
0.711MetTyr: 0.711 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
3.793AsnAla: 3.793 ± 0.451
0.553AsnCys: 0.553 ± 0.207
4.188AsnAsp: 4.188 ± 0.503
2.37AsnGlu: 2.37 ± 0.389
1.422AsnPhe: 1.422 ± 0.282
4.425AsnGly: 4.425 ± 0.727
0.948AsnHis: 0.948 ± 0.255
3.24AsnIle: 3.24 ± 0.483
2.686AsnLys: 2.686 ± 0.497
3.398AsnLeu: 3.398 ± 0.473
0.79AsnMet: 0.79 ± 0.228
2.607AsnAsn: 2.607 ± 0.484
1.659AsnPro: 1.659 ± 0.397
1.738AsnGln: 1.738 ± 0.379
2.449AsnArg: 2.449 ± 0.436
2.607AsnSer: 2.607 ± 0.497
2.686AsnThr: 2.686 ± 0.417
3.161AsnVal: 3.161 ± 0.528
0.869AsnTrp: 0.869 ± 0.24
1.975AsnTyr: 1.975 ± 0.5
0.0AsnXaa: 0.0 ± 0.0
Pro
3.793ProAla: 3.793 ± 0.588
0.474ProCys: 0.474 ± 0.213
3.082ProAsp: 3.082 ± 0.37
4.267ProGlu: 4.267 ± 0.556
1.422ProPhe: 1.422 ± 0.382
2.686ProGly: 2.686 ± 0.693
0.395ProHis: 0.395 ± 0.179
1.738ProIle: 1.738 ± 0.342
2.054ProLys: 2.054 ± 0.489
3.003ProLeu: 3.003 ± 0.489
1.185ProMet: 1.185 ± 0.355
2.212ProAsn: 2.212 ± 0.472
0.711ProPro: 0.711 ± 0.211
1.659ProGln: 1.659 ± 0.409
1.264ProArg: 1.264 ± 0.304
1.975ProSer: 1.975 ± 0.35
1.896ProThr: 1.896 ± 0.377
3.477ProVal: 3.477 ± 0.421
0.237ProTrp: 0.237 ± 0.155
1.501ProTyr: 1.501 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
4.662GlnAla: 4.662 ± 0.718
0.395GlnCys: 0.395 ± 0.16
2.212GlnAsp: 2.212 ± 0.494
2.054GlnGlu: 2.054 ± 0.428
1.58GlnPhe: 1.58 ± 0.383
2.133GlnGly: 2.133 ± 0.363
0.948GlnHis: 0.948 ± 0.301
3.082GlnIle: 3.082 ± 0.549
2.133GlnLys: 2.133 ± 0.519
2.845GlnLeu: 2.845 ± 0.456
1.738GlnMet: 1.738 ± 0.341
1.975GlnAsn: 1.975 ± 0.409
2.133GlnPro: 2.133 ± 0.355
2.528GlnGln: 2.528 ± 0.752
2.607GlnArg: 2.607 ± 0.514
1.817GlnSer: 1.817 ± 0.347
2.212GlnThr: 2.212 ± 0.426
2.37GlnVal: 2.37 ± 0.418
0.632GlnTrp: 0.632 ± 0.291
1.738GlnTyr: 1.738 ± 0.362
0.0GlnXaa: 0.0 ± 0.0
Arg
5.294ArgAla: 5.294 ± 0.565
0.553ArgCys: 0.553 ± 0.2
3.319ArgAsp: 3.319 ± 0.467
2.765ArgGlu: 2.765 ± 0.477
1.738ArgPhe: 1.738 ± 0.318
4.188ArgGly: 4.188 ± 0.465
0.948ArgHis: 0.948 ± 0.295
2.845ArgIle: 2.845 ± 0.49
3.793ArgLys: 3.793 ± 0.672
4.03ArgLeu: 4.03 ± 0.588
1.106ArgMet: 1.106 ± 0.307
2.924ArgAsn: 2.924 ± 0.481
1.817ArgPro: 1.817 ± 0.539
3.477ArgGln: 3.477 ± 0.546
3.635ArgArg: 3.635 ± 0.631
2.291ArgSer: 2.291 ± 0.368
3.003ArgThr: 3.003 ± 0.44
3.872ArgVal: 3.872 ± 0.492
0.553ArgTrp: 0.553 ± 0.252
1.185ArgTyr: 1.185 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
6.479SerAla: 6.479 ± 0.998
0.158SerCys: 0.158 ± 0.096
3.477SerAsp: 3.477 ± 0.549
2.924SerGlu: 2.924 ± 0.597
2.686SerPhe: 2.686 ± 0.534
5.215SerGly: 5.215 ± 0.715
1.58SerHis: 1.58 ± 0.361
2.37SerIle: 2.37 ± 0.436
3.161SerLys: 3.161 ± 0.485
4.978SerLeu: 4.978 ± 0.579
1.264SerMet: 1.264 ± 0.289
2.686SerAsn: 2.686 ± 0.393
2.607SerPro: 2.607 ± 0.505
2.37SerGln: 2.37 ± 0.551
2.765SerArg: 2.765 ± 0.514
2.765SerSer: 2.765 ± 0.495
4.267SerThr: 4.267 ± 0.707
4.346SerVal: 4.346 ± 0.725
0.869SerTrp: 0.869 ± 0.227
1.738SerTyr: 1.738 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
6.4ThrAla: 6.4 ± 0.745
0.553ThrCys: 0.553 ± 0.23
3.082ThrAsp: 3.082 ± 0.365
3.477ThrGlu: 3.477 ± 0.547
2.686ThrPhe: 2.686 ± 0.597
5.926ThrGly: 5.926 ± 0.783
0.474ThrHis: 0.474 ± 0.184
3.319ThrIle: 3.319 ± 0.492
2.686ThrLys: 2.686 ± 0.386
4.899ThrLeu: 4.899 ± 0.691
1.343ThrMet: 1.343 ± 0.374
1.738ThrAsn: 1.738 ± 0.329
3.793ThrPro: 3.793 ± 0.446
2.37ThrGln: 2.37 ± 0.48
3.003ThrArg: 3.003 ± 0.465
4.267ThrSer: 4.267 ± 0.729
3.793ThrThr: 3.793 ± 0.716
4.978ThrVal: 4.978 ± 0.619
0.632ThrTrp: 0.632 ± 0.255
2.291ThrTyr: 2.291 ± 0.514
0.0ThrXaa: 0.0 ± 0.0
Val
7.032ValAla: 7.032 ± 0.691
0.553ValCys: 0.553 ± 0.178
4.583ValAsp: 4.583 ± 0.523
4.82ValGlu: 4.82 ± 0.553
1.975ValPhe: 1.975 ± 0.586
4.188ValGly: 4.188 ± 0.527
1.027ValHis: 1.027 ± 0.297
4.188ValIle: 4.188 ± 0.535
4.741ValLys: 4.741 ± 0.76
4.425ValLeu: 4.425 ± 0.518
1.422ValMet: 1.422 ± 0.396
3.951ValAsn: 3.951 ± 0.651
2.291ValPro: 2.291 ± 0.565
2.449ValGln: 2.449 ± 0.399
3.793ValArg: 3.793 ± 0.557
4.583ValSer: 4.583 ± 0.642
5.373ValThr: 5.373 ± 0.663
5.057ValVal: 5.057 ± 0.782
1.027ValTrp: 1.027 ± 0.236
2.449ValTyr: 2.449 ± 0.369
0.0ValXaa: 0.0 ± 0.0
Trp
0.948TrpAla: 0.948 ± 0.344
0.079TrpCys: 0.079 ± 0.077
1.106TrpAsp: 1.106 ± 0.306
0.553TrpGlu: 0.553 ± 0.249
0.474TrpPhe: 0.474 ± 0.214
0.632TrpGly: 0.632 ± 0.21
0.395TrpHis: 0.395 ± 0.231
0.553TrpIle: 0.553 ± 0.19
0.395TrpLys: 0.395 ± 0.166
1.659TrpLeu: 1.659 ± 0.383
0.158TrpMet: 0.158 ± 0.11
0.711TrpAsn: 0.711 ± 0.239
0.395TrpPro: 0.395 ± 0.189
1.027TrpGln: 1.027 ± 0.267
1.106TrpArg: 1.106 ± 0.285
0.948TrpSer: 0.948 ± 0.249
0.948TrpThr: 0.948 ± 0.217
1.264TrpVal: 1.264 ± 0.301
0.158TrpTrp: 0.158 ± 0.108
0.553TrpTyr: 0.553 ± 0.205
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.398TyrAla: 3.398 ± 0.627
0.553TyrCys: 0.553 ± 0.218
2.449TyrAsp: 2.449 ± 0.501
2.528TyrGlu: 2.528 ± 0.462
1.343TyrPhe: 1.343 ± 0.388
2.924TyrGly: 2.924 ± 0.599
0.553TyrHis: 0.553 ± 0.194
1.106TyrIle: 1.106 ± 0.245
2.449TyrLys: 2.449 ± 0.485
2.133TyrLeu: 2.133 ± 0.291
1.027TyrMet: 1.027 ± 0.241
1.58TyrAsn: 1.58 ± 0.301
1.106TyrPro: 1.106 ± 0.328
1.58TyrGln: 1.58 ± 0.526
1.501TyrArg: 1.501 ± 0.289
1.817TyrSer: 1.817 ± 0.406
1.817TyrThr: 1.817 ± 0.368
1.58TyrVal: 1.58 ± 0.346
0.553TyrTrp: 0.553 ± 0.206
1.106TyrTyr: 1.106 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (12657 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski