Streptococcus uberis (strain ATCC BAA-854 / 0140J)
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B9DUL8|B9DUL8_STRU0 ABC transporter ATP-binding protein OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) OX=218495 GN=SUB1104 PE=4 SV=1
MM1 pKa = 7.75 TDD3 pKa = 4.62 FINLYY8 pKa = 10.66 DD9 pKa = 3.61 IVYY12 pKa = 10.68 DD13 pKa = 3.74 KK14 pKa = 10.49 NTHH17 pKa = 5.3 NKK19 pKa = 9.6 CIVVYY24 pKa = 10.8 VSDD27 pKa = 5.85 DD28 pKa = 4.08 IIDD31 pKa = 4.03 DD32 pKa = 4.12 SYY34 pKa = 11.8 LIDD37 pKa = 4.82 PIGYY41 pKa = 9.5 SFEE44 pKa = 4.66 LDD46 pKa = 2.82 WRR48 pKa = 11.84 EE49 pKa = 3.75 ASDD52 pKa = 4.67 LEE54 pKa = 4.41 KK55 pKa = 11.33 VPDD58 pKa = 3.73 NEE60 pKa = 4.19
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.49
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|B9DVW3|B9DVW3_STRU0 Sensor histidine kinase OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) OX=218495 GN=SUB1674 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1760
0
1760
535573
32
1483
304.3
34.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.336 ± 0.062
0.553 ± 0.015
5.693 ± 0.046
6.757 ± 0.063
4.738 ± 0.053
6.465 ± 0.06
2.036 ± 0.026
7.923 ± 0.054
7.111 ± 0.051
10.115 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.701 ± 0.027
4.607 ± 0.041
3.343 ± 0.029
4.027 ± 0.04
3.735 ± 0.041
6.387 ± 0.045
5.451 ± 0.04
6.56 ± 0.047
0.814 ± 0.021
3.649 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here