Amino acid dipepetide frequency for Thalassiosira pseudonana (Marine diatom) (Cyclotella nana)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.797AlaAla: 7.797 ± 0.064	1.177AlaCys: 1.177 ± 0.017	3.946AlaAsp: 3.946 ± 0.034	4.427AlaGlu: 4.427 ± 0.038	2.834AlaPhe: 2.834 ± 0.027	4.465AlaGly: 4.465 ± 0.034	1.356AlaHis: 1.356 ± 0.018	3.905AlaIle: 3.905 ± 0.031	4.38AlaLys: 4.38 ± 0.032	6.937AlaLeu: 6.937 ± 0.046	2.179AlaMet: 2.179 ± 0.018	3.35AlaAsn: 3.35 ± 0.026	3.442AlaPro: 3.442 ± 0.044	2.36AlaGln: 2.36 ± 0.023	3.667AlaArg: 3.667 ± 0.027	7.685AlaSer: 7.685 ± 0.045	5.384AlaThr: 5.384 ± 0.036	5.191AlaVal: 5.191 ± 0.031	0.778AlaTrp: 0.778 ± 0.015	1.808AlaTyr: 1.808 ± 0.018	0.0AlaXaa: 0.0 ± 0.0
Cys	1.076CysAla: 1.076 ± 0.015	0.49CysCys: 0.49 ± 0.013	0.997CysAsp: 0.997 ± 0.016	0.871CysGlu: 0.871 ± 0.014	0.64CysPhe: 0.64 ± 0.011	1.279CysGly: 1.279 ± 0.018	0.417CysHis: 0.417 ± 0.008	0.904CysIle: 0.904 ± 0.013	0.745CysLys: 0.745 ± 0.013	1.431CysLeu: 1.431 ± 0.017	0.338CysMet: 0.338 ± 0.008	0.763CysAsn: 0.763 ± 0.012	0.812CysPro: 0.812 ± 0.016	0.545CysGln: 0.545 ± 0.01	0.856CysArg: 0.856 ± 0.014	1.36CysSer: 1.36 ± 0.017	0.949CysThr: 0.949 ± 0.013	1.043CysVal: 1.043 ± 0.015	0.194CysTrp: 0.194 ± 0.006	0.47CysTyr: 0.47 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	5.298AspAla: 5.298 ± 0.034	0.945AspCys: 0.945 ± 0.016	6.351AspAsp: 6.351 ± 0.058	5.748AspGlu: 5.748 ± 0.045	2.095AspPhe: 2.095 ± 0.024	5.361AspGly: 5.361 ± 0.039	1.104AspHis: 1.104 ± 0.014	3.042AspIle: 3.042 ± 0.028	2.667AspLys: 2.667 ± 0.023	4.49AspLeu: 4.49 ± 0.031	1.412AspMet: 1.412 ± 0.017	2.769AspAsn: 2.769 ± 0.022	2.5AspPro: 2.5 ± 0.024	1.565AspGln: 1.565 ± 0.016	2.542AspArg: 2.542 ± 0.026	4.599AspSer: 4.599 ± 0.041	3.33AspThr: 3.33 ± 0.031	4.702AspVal: 4.702 ± 0.033	0.719AspTrp: 0.719 ± 0.013	1.614AspTyr: 1.614 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	5.046GluAla: 5.046 ± 0.038	1.034GluCys: 1.034 ± 0.016	4.68GluAsp: 4.68 ± 0.04	7.057GluGlu: 7.057 ± 0.067	1.942GluPhe: 1.942 ± 0.019	4.471GluGly: 4.471 ± 0.029	1.305GluHis: 1.305 ± 0.015	2.915GluIle: 2.915 ± 0.024	3.896GluLys: 3.896 ± 0.035	5.396GluLeu: 5.396 ± 0.039	1.853GluMet: 1.853 ± 0.019	2.535GluAsn: 2.535 ± 0.023	1.963GluPro: 1.963 ± 0.022	2.483GluGln: 2.483 ± 0.025	3.821GluArg: 3.821 ± 0.034	5.028GluSer: 5.028 ± 0.037	3.328GluThr: 3.328 ± 0.029	4.115GluVal: 4.115 ± 0.029	0.802GluTrp: 0.802 ± 0.012	1.9GluTyr: 1.9 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	2.756PheAla: 2.756 ± 0.024	0.657PheCys: 0.657 ± 0.014	2.528PheAsp: 2.528 ± 0.026	2.038PheGlu: 2.038 ± 0.022	1.465PhePhe: 1.465 ± 0.022	2.884PheGly: 2.884 ± 0.033	0.909PheHis: 0.909 ± 0.014	1.624PheIle: 1.624 ± 0.02	1.358PheLys: 1.358 ± 0.016	3.19PheLeu: 3.19 ± 0.029	0.798PheMet: 0.798 ± 0.012	1.45PheAsn: 1.45 ± 0.018	1.555PhePro: 1.555 ± 0.016	1.266PheGln: 1.266 ± 0.014	1.659PheArg: 1.659 ± 0.016	2.826PheSer: 2.826 ± 0.028	1.885PheThr: 1.885 ± 0.02	2.612PheVal: 2.612 ± 0.026	0.391PheTrp: 0.391 ± 0.009	1.02PheTyr: 1.02 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	4.727GlyAla: 4.727 ± 0.037	1.203GlyCys: 1.203 ± 0.017	4.653GlyAsp: 4.653 ± 0.035	4.41GlyGlu: 4.41 ± 0.028	2.396GlyPhe: 2.396 ± 0.024	8.181GlyGly: 8.181 ± 0.085	1.325GlyHis: 1.325 ± 0.015	3.398GlyIle: 3.398 ± 0.027	3.983GlyLys: 3.983 ± 0.03	5.002GlyLeu: 5.002 ± 0.033	1.964GlyMet: 1.964 ± 0.023	3.118GlyAsn: 3.118 ± 0.027	1.853GlyPro: 1.853 ± 0.022	1.868GlyGln: 1.868 ± 0.022	3.787GlyArg: 3.787 ± 0.032	6.158GlySer: 6.158 ± 0.047	3.781GlyThr: 3.781 ± 0.03	4.848GlyVal: 4.848 ± 0.035	0.93GlyTrp: 0.93 ± 0.014	1.986GlyTyr: 1.986 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	1.555HisAla: 1.555 ± 0.017	0.445HisCys: 0.445 ± 0.009	1.323HisAsp: 1.323 ± 0.014	1.258HisGlu: 1.258 ± 0.016	0.849HisPhe: 0.849 ± 0.014	1.518HisGly: 1.518 ± 0.02	1.133HisHis: 1.133 ± 0.02	1.027HisIle: 1.027 ± 0.013	0.943HisLys: 0.943 ± 0.014	2.041HisLeu: 2.041 ± 0.022	0.43HisMet: 0.43 ± 0.009	1.015HisAsn: 1.015 ± 0.014	1.506HisPro: 1.506 ± 0.016	1.164HisGln: 1.164 ± 0.018	1.476HisArg: 1.476 ± 0.018	1.958HisSer: 1.958 ± 0.022	1.115HisThr: 1.115 ± 0.015	1.388HisVal: 1.388 ± 0.017	0.28HisTrp: 0.28 ± 0.007	0.723HisTyr: 0.723 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.907IleAla: 3.907 ± 0.028	0.813IleCys: 0.813 ± 0.013	3.183IleAsp: 3.183 ± 0.026	2.934IleGlu: 2.934 ± 0.024	1.578IlePhe: 1.578 ± 0.018	3.283IleGly: 3.283 ± 0.027	1.202IleHis: 1.202 ± 0.015	2.299IleIle: 2.299 ± 0.022	2.193IleLys: 2.193 ± 0.021	4.232IleLeu: 4.232 ± 0.033	1.073IleMet: 1.073 ± 0.016	2.061IleAsn: 2.061 ± 0.022	2.469IlePro: 2.469 ± 0.025	1.876IleGln: 1.876 ± 0.02	2.389IleArg: 2.389 ± 0.022	4.004IleSer: 4.004 ± 0.029	2.714IleThr: 2.714 ± 0.027	3.396IleVal: 3.396 ± 0.026	0.441IleTrp: 0.441 ± 0.011	1.114IleTyr: 1.114 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	4.06LysAla: 4.06 ± 0.033	0.782LysCys: 0.782 ± 0.012	3.024LysAsp: 3.024 ± 0.028	4.193LysGlu: 4.193 ± 0.038	1.552LysPhe: 1.552 ± 0.017	3.246LysGly: 3.246 ± 0.031	1.224LysHis: 1.224 ± 0.016	2.262LysIle: 2.262 ± 0.022	4.503LysLys: 4.503 ± 0.042	4.354LysLeu: 4.354 ± 0.033	1.362LysMet: 1.362 ± 0.016	2.205LysAsn: 2.205 ± 0.021	2.197LysPro: 2.197 ± 0.028	2.409LysGln: 2.409 ± 0.026	3.702LysArg: 3.702 ± 0.035	4.652LysSer: 4.652 ± 0.038	2.75LysThr: 2.75 ± 0.025	3.071LysVal: 3.071 ± 0.025	0.604LysTrp: 0.604 ± 0.012	1.518LysTyr: 1.518 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	6.534LeuAla: 6.534 ± 0.036	1.459LeuCys: 1.459 ± 0.021	4.943LeuAsp: 4.943 ± 0.034	5.356LeuGlu: 5.356 ± 0.039	3.231LeuPhe: 3.231 ± 0.028	5.247LeuGly: 5.247 ± 0.038	2.13LeuHis: 2.13 ± 0.021	3.642LeuIle: 3.642 ± 0.029	4.36LeuLys: 4.36 ± 0.034	8.491LeuLeu: 8.491 ± 0.056	2.085LeuMet: 2.085 ± 0.02	3.438LeuAsn: 3.438 ± 0.024	4.572LeuPro: 4.572 ± 0.032	3.616LeuGln: 3.616 ± 0.03	4.822LeuArg: 4.822 ± 0.032	7.803LeuSer: 7.803 ± 0.051	4.489LeuThr: 4.489 ± 0.032	5.331LeuVal: 5.331 ± 0.038	0.896LeuTrp: 0.896 ± 0.015	2.166LeuTyr: 2.166 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.03MetAla: 2.03 ± 0.02	0.388MetCys: 0.388 ± 0.01	1.47MetAsp: 1.47 ± 0.016	1.798MetGlu: 1.798 ± 0.021	0.789MetPhe: 0.789 ± 0.014	1.644MetGly: 1.644 ± 0.02	0.572MetHis: 0.572 ± 0.011	1.179MetIle: 1.179 ± 0.014	1.663MetLys: 1.663 ± 0.018	2.038MetLeu: 2.038 ± 0.018	0.936MetMet: 0.936 ± 0.017	1.277MetAsn: 1.277 ± 0.016	1.163MetPro: 1.163 ± 0.019	1.011MetGln: 1.011 ± 0.016	1.209MetArg: 1.209 ± 0.015	2.194MetSer: 2.194 ± 0.018	1.46MetThr: 1.46 ± 0.016	1.406MetVal: 1.406 ± 0.016	0.246MetTrp: 0.246 ± 0.007	0.644MetTyr: 0.644 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.778AsnAla: 3.778 ± 0.026	0.619AsnCys: 0.619 ± 0.011	3.405AsnAsp: 3.405 ± 0.025	3.167AsnGlu: 3.167 ± 0.026	1.37AsnPhe: 1.37 ± 0.018	3.496AsnGly: 3.496 ± 0.03	1.017AsnHis: 1.017 ± 0.015	2.125AsnIle: 2.125 ± 0.017	2.207AsnLys: 2.207 ± 0.024	3.155AsnLeu: 3.155 ± 0.027	0.96AsnMet: 0.96 ± 0.012	3.359AsnAsn: 3.359 ± 0.042	2.169AsnPro: 2.169 ± 0.021	1.575AsnGln: 1.575 ± 0.018	1.996AsnArg: 1.996 ± 0.02	3.736AsnSer: 3.736 ± 0.028	2.788AsnThr: 2.788 ± 0.024	2.967AsnVal: 2.967 ± 0.023	0.453AsnTrp: 0.453 ± 0.009	1.156AsnTyr: 1.156 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	2.93ProAla: 2.93 ± 0.029	0.563ProCys: 0.563 ± 0.011	2.246ProAsp: 2.246 ± 0.023	2.406ProGlu: 2.406 ± 0.021	1.746ProPhe: 1.746 ± 0.018	2.282ProGly: 2.282 ± 0.024	1.108ProHis: 1.108 ± 0.016	2.258ProIle: 2.258 ± 0.021	2.36ProLys: 2.36 ± 0.023	4.052ProLeu: 4.052 ± 0.03	1.105ProMet: 1.105 ± 0.016	2.118ProAsn: 2.118 ± 0.022	4.071ProPro: 4.071 ± 0.05	1.714ProGln: 1.714 ± 0.023	2.176ProArg: 2.176 ± 0.024	6.056ProSer: 6.056 ± 0.132	3.894ProThr: 3.894 ± 0.047	2.688ProVal: 2.688 ± 0.026	0.488ProTrp: 0.488 ± 0.011	1.172ProTyr: 1.172 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	2.335GlnAla: 2.335 ± 0.021	0.618GlnCys: 0.618 ± 0.013	1.635GlnAsp: 1.635 ± 0.016	1.999GlnGlu: 1.999 ± 0.02	1.255GlnPhe: 1.255 ± 0.016	1.754GlnGly: 1.754 ± 0.02	1.263GlnHis: 1.263 ± 0.018	1.633GlnIle: 1.633 ± 0.016	1.933GlnLys: 1.933 ± 0.02	3.504GlnLeu: 3.504 ± 0.024	1.053GlnMet: 1.053 ± 0.017	1.457GlnAsn: 1.457 ± 0.018	2.011GlnPro: 2.011 ± 0.031	3.468GlnGln: 3.468 ± 0.059	2.635GlnArg: 2.635 ± 0.026	3.63GlnSer: 3.63 ± 0.031	2.083GlnThr: 2.083 ± 0.021	1.868GlnVal: 1.868 ± 0.02	0.487GlnTrp: 0.487 ± 0.01	1.206GlnTyr: 1.206 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	3.615ArgAla: 3.615 ± 0.032	0.877ArgCys: 0.877 ± 0.014	2.886ArgAsp: 2.886 ± 0.023	3.408ArgGlu: 3.408 ± 0.031	1.771ArgPhe: 1.771 ± 0.018	3.688ArgGly: 3.688 ± 0.036	1.428ArgHis: 1.428 ± 0.019	2.523ArgIle: 2.523 ± 0.025	3.276ArgLys: 3.276 ± 0.029	4.582ArgLeu: 4.582 ± 0.031	1.445ArgMet: 1.445 ± 0.018	2.286ArgAsn: 2.286 ± 0.021	2.17ArgPro: 2.17 ± 0.021	2.262ArgGln: 2.262 ± 0.022	4.56ArgArg: 4.56 ± 0.052	4.537ArgSer: 4.537 ± 0.035	2.598ArgThr: 2.598 ± 0.025	3.201ArgVal: 3.201 ± 0.025	0.67ArgTrp: 0.67 ± 0.011	1.531ArgTyr: 1.531 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.681SerAla: 6.681 ± 0.044	1.365SerCys: 1.365 ± 0.016	5.191SerAsp: 5.191 ± 0.045	4.592SerGlu: 4.592 ± 0.039	3.539SerPhe: 3.539 ± 0.032	6.002SerGly: 6.002 ± 0.051	1.951SerHis: 1.951 ± 0.021	4.587SerIle: 4.587 ± 0.034	4.788SerLys: 4.788 ± 0.034	7.872SerLeu: 7.872 ± 0.047	2.194SerMet: 2.194 ± 0.02	4.669SerAsn: 4.669 ± 0.034	4.941SerPro: 4.941 ± 0.063	3.046SerGln: 3.046 ± 0.03	4.33SerArg: 4.33 ± 0.037	12.838SerSer: 12.838 ± 0.108	6.563SerThr: 6.563 ± 0.041	5.44SerVal: 5.44 ± 0.035	0.94SerTrp: 0.94 ± 0.013	2.297SerTyr: 2.297 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	4.822ThrAla: 4.822 ± 0.035	0.932ThrCys: 0.932 ± 0.013	3.13ThrAsp: 3.13 ± 0.022	3.049ThrGlu: 3.049 ± 0.025	2.052ThrPhe: 2.052 ± 0.02	3.436ThrGly: 3.436 ± 0.032	1.208ThrHis: 1.208 ± 0.014	3.15ThrIle: 3.15 ± 0.024	3.299ThrLys: 3.299 ± 0.025	5.082ThrLeu: 5.082 ± 0.037	1.475ThrMet: 1.475 ± 0.017	3.033ThrAsn: 3.033 ± 0.022	3.483ThrPro: 3.483 ± 0.032	1.927ThrGln: 1.927 ± 0.021	2.576ThrArg: 2.576 ± 0.021	6.419ThrSer: 6.419 ± 0.039	5.347ThrThr: 5.347 ± 0.046	3.713ThrVal: 3.713 ± 0.028	0.592ThrTrp: 0.592 ± 0.011	1.362ThrTyr: 1.362 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	5.533ValAla: 5.533 ± 0.038	1.05ValCys: 1.05 ± 0.014	4.561ValAsp: 4.561 ± 0.033	4.389ValGlu: 4.389 ± 0.031	2.232ValPhe: 2.232 ± 0.022	4.611ValGly: 4.611 ± 0.031	1.467ValHis: 1.467 ± 0.017	2.945ValIle: 2.945 ± 0.023	3.188ValLys: 3.188 ± 0.026	5.495ValLeu: 5.495 ± 0.038	1.539ValMet: 1.539 ± 0.014	2.658ValAsn: 2.658 ± 0.021	3.0ValPro: 3.0 ± 0.028	2.238ValGln: 2.238 ± 0.018	3.021ValArg: 3.021 ± 0.024	5.343ValSer: 5.343 ± 0.032	3.543ValThr: 3.543 ± 0.03	5.071ValVal: 5.071 ± 0.037	0.715ValTrp: 0.715 ± 0.013	1.691ValTyr: 1.691 ± 0.016	0.0ValXaa: 0.0 ± 0.0
Trp	0.684TrpAla: 0.684 ± 0.012	0.218TrpCys: 0.218 ± 0.007	0.718TrpAsp: 0.718 ± 0.012	0.721TrpGlu: 0.721 ± 0.01	0.408TrpPhe: 0.408 ± 0.009	0.767TrpGly: 0.767 ± 0.013	0.245TrpHis: 0.245 ± 0.007	0.589TrpIle: 0.589 ± 0.01	0.775TrpLys: 0.775 ± 0.013	0.924TrpLeu: 0.924 ± 0.014	0.378TrpMet: 0.378 ± 0.008	0.604TrpAsn: 0.604 ± 0.011	0.341TrpPro: 0.341 ± 0.008	0.355TrpGln: 0.355 ± 0.008	0.702TrpArg: 0.702 ± 0.012	0.868TrpSer: 0.868 ± 0.013	0.641TrpThr: 0.641 ± 0.011	0.635TrpVal: 0.635 ± 0.012	0.191TrpTrp: 0.191 ± 0.006	0.384TrpTyr: 0.384 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.906TyrAla: 1.906 ± 0.018	0.544TyrCys: 0.544 ± 0.009	1.983TyrAsp: 1.983 ± 0.023	1.749TyrGlu: 1.749 ± 0.02	1.128TyrPhe: 1.128 ± 0.015	1.957TyrGly: 1.957 ± 0.019	0.783TyrHis: 0.783 ± 0.012	1.152TyrIle: 1.152 ± 0.015	1.153TyrLys: 1.153 ± 0.014	2.343TyrLeu: 2.343 ± 0.027	0.537TyrMet: 0.537 ± 0.011	1.333TyrAsn: 1.333 ± 0.017	1.214TyrPro: 1.214 ± 0.017	1.079TyrGln: 1.079 ± 0.014	1.387TyrArg: 1.387 ± 0.017	2.132TyrSer: 2.132 ± 0.019	1.446TyrThr: 1.446 ± 0.016	1.604TyrVal: 1.604 ± 0.019	0.33TyrTrp: 0.33 ± 0.008	1.002TyrTyr: 1.002 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11718 proteins (5824029 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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