Salinivibrio sp. KP-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Salinivibrio; unclassified Salinivibrio

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5AU70|A0A0F5AU70_9GAMM Peptidase M13 OS=Salinivibrio sp. KP-1 OX=1406902 GN=WN56_00080 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.43KK3 pKa = 9.02TILAMAVPALLAAGSASASINLYY26 pKa = 9.87DD27 pKa = 3.95AEE29 pKa = 4.44GVKK32 pKa = 10.3VDD34 pKa = 3.57ASGAAEE40 pKa = 3.93VQYY43 pKa = 10.43FQAIGEE49 pKa = 4.37NKK51 pKa = 10.09DD52 pKa = 3.46GEE54 pKa = 4.32WRR56 pKa = 11.84LDD58 pKa = 3.83DD59 pKa = 5.06GDD61 pKa = 3.97FAVNTEE67 pKa = 4.22VAVSEE72 pKa = 4.05DD73 pKa = 3.32LAAIGHH79 pKa = 6.11FAFKK83 pKa = 10.9FEE85 pKa = 5.95DD86 pKa = 3.68STTKK90 pKa = 10.58NDD92 pKa = 3.17EE93 pKa = 4.34LYY95 pKa = 11.2VGFKK99 pKa = 10.7GSDD102 pKa = 2.59WGTLTFGRR110 pKa = 11.84QLLINDD116 pKa = 4.16DD117 pKa = 3.54MGITKK122 pKa = 10.19DD123 pKa = 3.89YY124 pKa = 10.2EE125 pKa = 4.0LSSASNGTLRR135 pKa = 11.84TEE137 pKa = 4.72GDD139 pKa = 3.33QVAKK143 pKa = 10.46YY144 pKa = 10.51VYY146 pKa = 10.88DD147 pKa = 3.42NGTFYY152 pKa = 11.22AGLSTLQNSDD162 pKa = 3.52GEE164 pKa = 4.4EE165 pKa = 3.94SSDD168 pKa = 3.49EE169 pKa = 3.88YY170 pKa = 11.12KK171 pKa = 10.46IYY173 pKa = 10.49DD174 pKa = 3.17GRR176 pKa = 11.84LGYY179 pKa = 10.17RR180 pKa = 11.84VADD183 pKa = 3.01VDD185 pKa = 3.57MRR187 pKa = 11.84VYY189 pKa = 10.73YY190 pKa = 10.43YY191 pKa = 10.68YY192 pKa = 11.34ADD194 pKa = 3.96DD195 pKa = 4.97AGVNTAGDD203 pKa = 3.87DD204 pKa = 3.48FVYY207 pKa = 10.65ASKK210 pKa = 11.45GDD212 pKa = 3.53VEE214 pKa = 4.54NYY216 pKa = 10.06NFEE219 pKa = 4.04VEE221 pKa = 4.24YY222 pKa = 11.09AGIEE226 pKa = 4.23NVSLAAAYY234 pKa = 9.08GVNTVDD240 pKa = 3.61TGSSDD245 pKa = 3.37TEE247 pKa = 3.82ADD249 pKa = 3.96FFSVAADD256 pKa = 3.62YY257 pKa = 9.45THH259 pKa = 6.99GKK261 pKa = 4.82TTYY264 pKa = 10.86AIGIDD269 pKa = 3.6NKK271 pKa = 11.37DD272 pKa = 3.31NDD274 pKa = 3.8NEE276 pKa = 4.09EE277 pKa = 4.84DD278 pKa = 3.6VTGYY282 pKa = 9.43YY283 pKa = 11.12ANVTYY288 pKa = 10.86ALHH291 pKa = 6.63NNAKK295 pKa = 9.88VYY297 pKa = 11.25AEE299 pKa = 4.34VGDD302 pKa = 4.05NDD304 pKa = 4.92ADD306 pKa = 4.06NNDD309 pKa = 3.23WGYY312 pKa = 9.36VAGMEE317 pKa = 4.59VKK319 pKa = 10.61FF320 pKa = 4.19

Molecular weight: 34.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5AQ48|A0A0F5AQ48_9GAMM DNA polymerase III subunit psi OS=Salinivibrio sp. KP-1 OX=1406902 GN=WN56_11480 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.44VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 8.23QLSKK44 pKa = 11.23

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3027

0

3027

976084

35

2095

322.5

35.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.92 ± 0.05

1.014 ± 0.015

5.943 ± 0.045

5.812 ± 0.037

3.735 ± 0.028

7.022 ± 0.041

2.521 ± 0.026

5.555 ± 0.033

4.297 ± 0.033

10.362 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.023

3.525 ± 0.025

4.257 ± 0.025

5.051 ± 0.041

5.308 ± 0.034

6.013 ± 0.036

5.508 ± 0.034

7.249 ± 0.038

1.324 ± 0.018

2.879 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski