Lachnospiraceae bacterium NK3A20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3ZQ01|A0A1H3ZQ01_9FIRM CxxC motif-containing protein OS=Lachnospiraceae bacterium NK3A20 OX=877406 GN=SAMN02745687_02306 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.36KK3 pKa = 9.79RR4 pKa = 11.84VLSIVLGTAMAASVLAGCGSTDD26 pKa = 3.25SAEE29 pKa = 4.41PAATAAPADD38 pKa = 4.13ADD40 pKa = 4.02TAATEE45 pKa = 4.14AATDD49 pKa = 3.62AATTAAEE56 pKa = 4.67GNTEE60 pKa = 4.07TATTEE65 pKa = 3.72AAADD69 pKa = 3.93AEE71 pKa = 4.63AGDD74 pKa = 3.95IPGGRR79 pKa = 11.84VYY81 pKa = 11.22LLNFKK86 pKa = 10.66PEE88 pKa = 4.19TDD90 pKa = 5.07DD91 pKa = 3.36AWQDD95 pKa = 3.39LASTYY100 pKa = 11.25NGMGGNVTVVTAADD114 pKa = 3.7GQYY117 pKa = 9.85ATTLQSEE124 pKa = 4.66MAKK127 pKa = 10.15SEE129 pKa = 3.9APTIFNIGNSTDD141 pKa = 3.07AQTWDD146 pKa = 4.09DD147 pKa = 3.6YY148 pKa = 11.75TYY150 pKa = 10.95DD151 pKa = 3.59LKK153 pKa = 11.49GSAVYY158 pKa = 10.55DD159 pKa = 3.68HH160 pKa = 7.07VSDD163 pKa = 4.17HH164 pKa = 6.66SLDD167 pKa = 3.37VLYY170 pKa = 10.82NGKK173 pKa = 7.7TAAIANCYY181 pKa = 7.38EE182 pKa = 4.23AYY184 pKa = 10.45GIIYY188 pKa = 10.35NKK190 pKa = 9.8TILDD194 pKa = 4.44AYY196 pKa = 8.99CTLDD200 pKa = 3.36GAKK203 pKa = 9.85VASADD208 pKa = 3.97EE209 pKa = 4.27IQDD212 pKa = 3.42FDD214 pKa = 4.1TLKK217 pKa = 11.06AVADD221 pKa = 4.95DD222 pKa = 3.44INSRR226 pKa = 11.84VDD228 pKa = 3.63EE229 pKa = 4.64LNEE232 pKa = 4.07ALAGTAFDD240 pKa = 4.01GTVTEE245 pKa = 4.92AFASAGLDD253 pKa = 3.7DD254 pKa = 4.15SSSWRR259 pKa = 11.84FSGHH263 pKa = 6.45LANFPLYY270 pKa = 11.0YY271 pKa = 10.18EE272 pKa = 4.65FKK274 pKa = 10.8DD275 pKa = 5.16DD276 pKa = 4.37GLEE279 pKa = 4.15DD280 pKa = 3.64LTAGEE285 pKa = 4.53PEE287 pKa = 4.03IKK289 pKa = 9.38GTYY292 pKa = 9.03LDD294 pKa = 3.85QYY296 pKa = 9.73QKK298 pKa = 10.65VWDD301 pKa = 4.01MYY303 pKa = 10.82VADD306 pKa = 4.45SAADD310 pKa = 3.65PATLAGGTYY319 pKa = 9.73NAEE322 pKa = 4.12EE323 pKa = 3.96EE324 pKa = 4.53LGLGEE329 pKa = 4.94AVFYY333 pKa = 11.32QNGDD337 pKa = 3.32WEE339 pKa = 5.13FSALTNPEE347 pKa = 3.17NGYY350 pKa = 10.03IVTADD355 pKa = 4.8DD356 pKa = 4.82LDD358 pKa = 3.58MMPIYY363 pKa = 10.58FGVDD367 pKa = 3.36DD368 pKa = 4.76EE369 pKa = 5.0NEE371 pKa = 4.0GLAVGTEE378 pKa = 4.01NHH380 pKa = 5.77WAVNSKK386 pKa = 10.08AAQEE390 pKa = 5.11DD391 pKa = 3.7IDD393 pKa = 4.11ASLYY397 pKa = 9.18FLNWAITSDD406 pKa = 3.64EE407 pKa = 3.99GRR409 pKa = 11.84DD410 pKa = 3.67AIVNTMGLSAPFDD423 pKa = 3.75TFTGDD428 pKa = 3.9FAPANAFGAKK438 pKa = 9.49AAVYY442 pKa = 9.07TEE444 pKa = 4.11AGKK447 pKa = 8.71TSTAWSFNATPNVDD461 pKa = 3.0DD462 pKa = 3.57WRR464 pKa = 11.84ADD466 pKa = 3.45VVSALTAYY474 pKa = 9.73TDD476 pKa = 4.83GSGDD480 pKa = 3.08WDD482 pKa = 3.63AVKK485 pKa = 10.64DD486 pKa = 3.81AFVTGWADD494 pKa = 3.23QWRR497 pKa = 11.84LAHH500 pKa = 7.14AEE502 pKa = 3.9

Molecular weight: 53.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3WT17|A0A1H3WT17_9FIRM Uncharacterized protein PH0010 family/AmmeMemoRadiSam system protein A/AmmeMemoRadiSam system protein B OS=Lachnospiraceae bacterium NK3A20 OX=877406 GN=SAMN02745687_01019 PE=4 SV=1
MM1 pKa = 7.64SKK3 pKa = 7.87MTLQPKK9 pKa = 9.35KK10 pKa = 8.73LQRR13 pKa = 11.84SRR15 pKa = 11.84VHH17 pKa = 6.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.38AGRR29 pKa = 11.84KK30 pKa = 8.69VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.26GRR40 pKa = 11.84AKK42 pKa = 10.1LTVV45 pKa = 3.04

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2503

0

2503

869147

39

1981

347.2

38.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.965 ± 0.059

1.298 ± 0.018

6.285 ± 0.044

6.951 ± 0.053

3.958 ± 0.034

7.455 ± 0.041

1.984 ± 0.021

6.718 ± 0.044

5.216 ± 0.042

8.772 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.022

3.98 ± 0.029

3.709 ± 0.026

3.273 ± 0.029

5.418 ± 0.043

5.713 ± 0.037

5.736 ± 0.041

6.625 ± 0.04

1.043 ± 0.018

4.006 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski