Amino acid dipepetide frequency for Lachnospiraceae bacterium NK3A20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.705AlaAla: 11.705 ± 0.213	1.138AlaCys: 1.138 ± 0.037	5.981AlaAsp: 5.981 ± 0.103	6.189AlaGlu: 6.189 ± 0.094	3.308AlaPhe: 3.308 ± 0.061	7.697AlaGly: 7.697 ± 0.115	1.392AlaHis: 1.392 ± 0.039	5.331AlaIle: 5.331 ± 0.09	4.853AlaLys: 4.853 ± 0.085	7.603AlaLeu: 7.603 ± 0.125	2.661AlaMet: 2.661 ± 0.062	2.914AlaAsn: 2.914 ± 0.061	2.743AlaPro: 2.743 ± 0.064	2.905AlaGln: 2.905 ± 0.061	4.076AlaArg: 4.076 ± 0.067	4.558AlaSer: 4.558 ± 0.071	4.148AlaThr: 4.148 ± 0.089	6.692AlaVal: 6.692 ± 0.12	0.826AlaTrp: 0.826 ± 0.035	2.93AlaTyr: 2.93 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	1.075CysAla: 1.075 ± 0.035	0.232CysCys: 0.232 ± 0.017	0.872CysAsp: 0.872 ± 0.037	0.763CysGlu: 0.763 ± 0.03	0.46CysPhe: 0.46 ± 0.025	1.383CysGly: 1.383 ± 0.045	0.326CysHis: 0.326 ± 0.022	0.899CysIle: 0.899 ± 0.035	0.479CysLys: 0.479 ± 0.024	1.068CysLeu: 1.068 ± 0.037	0.365CysMet: 0.365 ± 0.02	0.471CysAsn: 0.471 ± 0.025	0.57CysPro: 0.57 ± 0.028	0.329CysGln: 0.329 ± 0.019	0.786CysArg: 0.786 ± 0.036	0.706CysSer: 0.706 ± 0.031	0.672CysThr: 0.672 ± 0.032	0.902CysVal: 0.902 ± 0.034	0.132CysTrp: 0.132 ± 0.013	0.496CysTyr: 0.496 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	5.653AspAla: 5.653 ± 0.1	0.709AspCys: 0.709 ± 0.034	3.526AspAsp: 3.526 ± 0.081	4.892AspGlu: 4.892 ± 0.086	2.553AspPhe: 2.553 ± 0.065	5.268AspGly: 5.268 ± 0.106	1.39AspHis: 1.39 ± 0.045	4.168AspIle: 4.168 ± 0.072	3.074AspLys: 3.074 ± 0.067	5.173AspLeu: 5.173 ± 0.091	1.751AspMet: 1.751 ± 0.042	2.424AspAsn: 2.424 ± 0.059	2.644AspPro: 2.644 ± 0.061	1.805AspGln: 1.805 ± 0.051	3.272AspArg: 3.272 ± 0.063	3.083AspSer: 3.083 ± 0.075	3.532AspThr: 3.532 ± 0.073	4.11AspVal: 4.11 ± 0.077	0.782AspTrp: 0.782 ± 0.034	3.04AspTyr: 3.04 ± 0.064	0.0AspXaa: 0.0 ± 0.0
Glu	6.04GluAla: 6.04 ± 0.096	0.627GluCys: 0.627 ± 0.03	4.465GluAsp: 4.465 ± 0.075	6.443GluGlu: 6.443 ± 0.106	2.282GluPhe: 2.282 ± 0.05	4.577GluGly: 4.577 ± 0.064	1.397GluHis: 1.397 ± 0.038	4.671GluIle: 4.671 ± 0.078	5.195GluLys: 5.195 ± 0.089	5.895GluLeu: 5.895 ± 0.095	2.096GluMet: 2.096 ± 0.051	3.402GluAsn: 3.402 ± 0.064	1.993GluPro: 1.993 ± 0.051	2.578GluGln: 2.578 ± 0.062	3.668GluArg: 3.668 ± 0.075	3.274GluSer: 3.274 ± 0.07	3.778GluThr: 3.778 ± 0.077	3.935GluVal: 3.935 ± 0.084	0.58GluTrp: 0.58 ± 0.026	2.616GluTyr: 2.616 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	3.345PheAla: 3.345 ± 0.07	0.66PheCys: 0.66 ± 0.029	2.662PheAsp: 2.662 ± 0.053	2.256PheGlu: 2.256 ± 0.047	1.627PhePhe: 1.627 ± 0.052	3.257PheGly: 3.257 ± 0.072	0.963PheHis: 0.963 ± 0.032	2.486PheIle: 2.486 ± 0.065	1.339PheLys: 1.339 ± 0.039	3.905PheLeu: 3.905 ± 0.089	1.073PheMet: 1.073 ± 0.034	1.414PheAsn: 1.414 ± 0.037	1.453PhePro: 1.453 ± 0.044	1.154PheGln: 1.154 ± 0.036	2.352PheArg: 2.352 ± 0.057	2.531PheSer: 2.531 ± 0.06	2.448PheThr: 2.448 ± 0.053	2.538PheVal: 2.538 ± 0.061	0.5PheTrp: 0.5 ± 0.024	1.612PheTyr: 1.612 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	6.044GlyAla: 6.044 ± 0.099	1.206GlyCys: 1.206 ± 0.039	4.188GlyAsp: 4.188 ± 0.071	4.858GlyGlu: 4.858 ± 0.073	3.067GlyPhe: 3.067 ± 0.064	5.241GlyGly: 5.241 ± 0.096	1.4GlyHis: 1.4 ± 0.041	5.962GlyIle: 5.962 ± 0.087	4.377GlyLys: 4.377 ± 0.091	5.88GlyLeu: 5.88 ± 0.099	2.478GlyMet: 2.478 ± 0.049	3.052GlyAsn: 3.052 ± 0.066	1.58GlyPro: 1.58 ± 0.044	2.377GlyGln: 2.377 ± 0.056	4.34GlyArg: 4.34 ± 0.069	4.672GlySer: 4.672 ± 0.083	4.761GlyThr: 4.761 ± 0.082	4.874GlyVal: 4.874 ± 0.078	0.845GlyTrp: 0.845 ± 0.035	3.35GlyTyr: 3.35 ± 0.066	0.0GlyXaa: 0.0 ± 0.0
His	1.52HisAla: 1.52 ± 0.042	0.303HisCys: 0.303 ± 0.019	1.203HisAsp: 1.203 ± 0.051	1.248HisGlu: 1.248 ± 0.038	0.947HisPhe: 0.947 ± 0.031	1.643HisGly: 1.643 ± 0.041	0.575HisHis: 0.575 ± 0.056	1.292HisIle: 1.292 ± 0.042	0.802HisLys: 0.802 ± 0.031	1.648HisLeu: 1.648 ± 0.051	0.521HisMet: 0.521 ± 0.023	0.697HisAsn: 0.697 ± 0.027	1.083HisPro: 1.083 ± 0.036	0.611HisGln: 0.611 ± 0.028	1.159HisArg: 1.159 ± 0.039	1.063HisSer: 1.063 ± 0.034	1.126HisThr: 1.126 ± 0.038	1.296HisVal: 1.296 ± 0.042	0.197HisTrp: 0.197 ± 0.016	0.902HisTyr: 0.902 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.22IleAla: 6.22 ± 0.098	0.996IleCys: 0.996 ± 0.038	4.079IleAsp: 4.079 ± 0.089	3.826IleGlu: 3.826 ± 0.079	2.745IlePhe: 2.745 ± 0.06	4.88IleGly: 4.88 ± 0.083	1.487IleHis: 1.487 ± 0.042	4.109IleIle: 4.109 ± 0.085	2.265IleLys: 2.265 ± 0.058	6.769IleLeu: 6.769 ± 0.116	1.726IleMet: 1.726 ± 0.049	2.47IleAsn: 2.47 ± 0.057	3.193IlePro: 3.193 ± 0.063	1.9IleGln: 1.9 ± 0.046	4.391IleArg: 4.391 ± 0.08	4.143IleSer: 4.143 ± 0.071	4.28IleThr: 4.28 ± 0.08	4.567IleVal: 4.567 ± 0.083	0.635IleTrp: 0.635 ± 0.028	2.505IleTyr: 2.505 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	4.934LysAla: 4.934 ± 0.089	0.502LysCys: 0.502 ± 0.021	3.533LysAsp: 3.533 ± 0.071	4.496LysGlu: 4.496 ± 0.081	1.665LysPhe: 1.665 ± 0.044	3.367LysGly: 3.367 ± 0.058	0.655LysHis: 0.655 ± 0.029	3.597LysIle: 3.597 ± 0.072	3.582LysLys: 3.582 ± 0.087	4.349LysLeu: 4.349 ± 0.081	1.497LysMet: 1.497 ± 0.043	2.164LysAsn: 2.164 ± 0.049	1.809LysPro: 1.809 ± 0.046	1.31LysGln: 1.31 ± 0.046	2.879LysArg: 2.879 ± 0.056	2.667LysSer: 2.667 ± 0.062	3.157LysThr: 3.157 ± 0.074	3.426LysVal: 3.426 ± 0.073	0.536LysTrp: 0.536 ± 0.026	2.039LysTyr: 2.039 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	7.35LeuAla: 7.35 ± 0.104	1.327LeuCys: 1.327 ± 0.044	5.212LeuAsp: 5.212 ± 0.094	5.044LeuGlu: 5.044 ± 0.089	3.644LeuPhe: 3.644 ± 0.087	5.733LeuGly: 5.733 ± 0.087	1.894LeuHis: 1.894 ± 0.047	5.871LeuIle: 5.871 ± 0.106	4.005LeuLys: 4.005 ± 0.078	8.271LeuLeu: 8.271 ± 0.139	2.439LeuMet: 2.439 ± 0.056	3.36LeuAsn: 3.36 ± 0.059	4.075LeuPro: 4.075 ± 0.072	3.087LeuGln: 3.087 ± 0.06	4.969LeuArg: 4.969 ± 0.09	6.473LeuSer: 6.473 ± 0.114	5.265LeuThr: 5.265 ± 0.086	5.441LeuVal: 5.441 ± 0.087	0.872LeuTrp: 0.872 ± 0.036	3.386LeuTyr: 3.386 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.682MetAla: 2.682 ± 0.058	0.299MetCys: 0.299 ± 0.017	1.89MetAsp: 1.89 ± 0.043	1.892MetGlu: 1.892 ± 0.053	0.976MetPhe: 0.976 ± 0.037	1.796MetGly: 1.796 ± 0.047	0.51MetHis: 0.51 ± 0.026	2.009MetIle: 2.009 ± 0.051	1.969MetLys: 1.969 ± 0.053	2.436MetLeu: 2.436 ± 0.066	0.905MetMet: 0.905 ± 0.031	1.408MetAsn: 1.408 ± 0.037	1.205MetPro: 1.205 ± 0.035	1.161MetGln: 1.161 ± 0.037	1.482MetArg: 1.482 ± 0.043	1.756MetSer: 1.756 ± 0.042	1.871MetThr: 1.871 ± 0.05	1.646MetVal: 1.646 ± 0.04	0.237MetTrp: 0.237 ± 0.017	0.805MetTyr: 0.805 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	3.537AsnAla: 3.537 ± 0.077	0.524AsnCys: 0.524 ± 0.029	2.181AsnAsp: 2.181 ± 0.065	2.545AsnGlu: 2.545 ± 0.051	1.596AsnPhe: 1.596 ± 0.041	3.485AsnGly: 3.485 ± 0.076	0.804AsnHis: 0.804 ± 0.032	2.817AsnIle: 2.817 ± 0.063	1.713AsnLys: 1.713 ± 0.052	3.402AsnLeu: 3.402 ± 0.064	1.163AsnMet: 1.163 ± 0.037	1.553AsnAsn: 1.553 ± 0.051	1.981AsnPro: 1.981 ± 0.051	1.134AsnGln: 1.134 ± 0.037	2.16AsnArg: 2.16 ± 0.052	2.134AsnSer: 2.134 ± 0.057	2.305AsnThr: 2.305 ± 0.058	2.693AsnVal: 2.693 ± 0.056	0.444AsnTrp: 0.444 ± 0.026	1.629AsnTyr: 1.629 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	3.428ProAla: 3.428 ± 0.077	0.45ProCys: 0.45 ± 0.022	2.936ProAsp: 2.936 ± 0.06	3.806ProGlu: 3.806 ± 0.061	1.622ProPhe: 1.622 ± 0.042	2.907ProGly: 2.907 ± 0.062	0.703ProHis: 0.703 ± 0.033	2.049ProIle: 2.049 ± 0.046	1.813ProLys: 1.813 ± 0.048	2.919ProLeu: 2.919 ± 0.06	0.942ProMet: 0.942 ± 0.036	1.228ProAsn: 1.228 ± 0.037	0.761ProPro: 0.761 ± 0.032	1.155ProGln: 1.155 ± 0.037	1.369ProArg: 1.369 ± 0.039	2.057ProSer: 2.057 ± 0.049	1.72ProThr: 1.72 ± 0.053	3.184ProVal: 3.184 ± 0.065	0.44ProTrp: 0.44 ± 0.023	1.605ProTyr: 1.605 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.78GlnAla: 2.78 ± 0.054	0.315GlnCys: 0.315 ± 0.018	1.944GlnAsp: 1.944 ± 0.047	2.3GlnGlu: 2.3 ± 0.057	1.146GlnPhe: 1.146 ± 0.034	2.031GlnGly: 2.031 ± 0.047	0.656GlnHis: 0.656 ± 0.025	2.165GlnIle: 2.165 ± 0.053	2.247GlnLys: 2.247 ± 0.042	2.848GlnLeu: 2.848 ± 0.064	1.092GlnMet: 1.092 ± 0.035	1.63GlnAsn: 1.63 ± 0.049	1.128GlnPro: 1.128 ± 0.039	1.535GlnGln: 1.535 ± 0.054	1.688GlnArg: 1.688 ± 0.048	1.78GlnSer: 1.78 ± 0.048	1.864GlnThr: 1.864 ± 0.047	1.764GlnVal: 1.764 ± 0.046	0.3GlnTrp: 0.3 ± 0.018	1.518GlnTyr: 1.518 ± 0.048	0.0GlnXaa: 0.0 ± 0.0
Arg	3.996ArgAla: 3.996 ± 0.066	0.622ArgCys: 0.622 ± 0.027	3.21ArgAsp: 3.21 ± 0.064	4.479ArgGlu: 4.479 ± 0.079	2.133ArgPhe: 2.133 ± 0.054	3.263ArgGly: 3.263 ± 0.067	1.172ArgHis: 1.172 ± 0.04	4.13ArgIle: 4.13 ± 0.073	3.429ArgLys: 3.429 ± 0.071	4.573ArgLeu: 4.573 ± 0.078	1.804ArgMet: 1.804 ± 0.046	2.432ArgAsn: 2.432 ± 0.06	1.779ArgPro: 1.779 ± 0.048	2.24ArgGln: 2.24 ± 0.056	3.312ArgArg: 3.312 ± 0.067	2.687ArgSer: 2.687 ± 0.062	2.916ArgThr: 2.916 ± 0.068	3.246ArgVal: 3.246 ± 0.063	0.495ArgTrp: 0.495 ± 0.025	2.263ArgTyr: 2.263 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	5.014SerAla: 5.014 ± 0.085	0.689SerCys: 0.689 ± 0.025	3.755SerAsp: 3.755 ± 0.075	3.66SerGlu: 3.66 ± 0.078	2.551SerPhe: 2.551 ± 0.051	5.144SerGly: 5.144 ± 0.087	1.105SerHis: 1.105 ± 0.04	3.666SerIle: 3.666 ± 0.073	2.529SerLys: 2.529 ± 0.064	5.156SerLeu: 5.156 ± 0.105	1.694SerMet: 1.694 ± 0.041	2.094SerAsn: 2.094 ± 0.049	1.881SerPro: 1.881 ± 0.046	1.729SerGln: 1.729 ± 0.053	3.105SerArg: 3.105 ± 0.061	3.281SerSer: 3.281 ± 0.073	2.966SerThr: 2.966 ± 0.067	4.125SerVal: 4.125 ± 0.074	0.696SerTrp: 0.696 ± 0.031	2.291SerTyr: 2.291 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	5.565ThrAla: 5.565 ± 0.094	0.644ThrCys: 0.644 ± 0.025	3.986ThrAsp: 3.986 ± 0.084	3.757ThrGlu: 3.757 ± 0.088	2.273ThrPhe: 2.273 ± 0.056	5.092ThrGly: 5.092 ± 0.08	0.942ThrHis: 0.942 ± 0.032	3.995ThrIle: 3.995 ± 0.076	2.887ThrLys: 2.887 ± 0.062	4.827ThrLeu: 4.827 ± 0.076	1.445ThrMet: 1.445 ± 0.04	2.073ThrAsn: 2.073 ± 0.057	2.493ThrPro: 2.493 ± 0.071	1.594ThrGln: 1.594 ± 0.042	2.53ThrArg: 2.53 ± 0.053	3.15ThrSer: 3.15 ± 0.067	3.087ThrThr: 3.087 ± 0.067	4.349ThrVal: 4.349 ± 0.073	0.614ThrTrp: 0.614 ± 0.029	2.057ThrTyr: 2.057 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	5.102ValAla: 5.102 ± 0.087	0.994ValCys: 0.994 ± 0.035	3.805ValAsp: 3.805 ± 0.086	3.694ValGlu: 3.694 ± 0.08	2.833ValPhe: 2.833 ± 0.06	4.135ValGly: 4.135 ± 0.074	1.197ValHis: 1.197 ± 0.041	4.831ValIle: 4.831 ± 0.081	3.375ValLys: 3.375 ± 0.072	6.165ValLeu: 6.165 ± 0.093	1.977ValMet: 1.977 ± 0.052	2.745ValAsn: 2.745 ± 0.062	2.833ValPro: 2.833 ± 0.066	2.089ValGln: 2.089 ± 0.043	3.708ValArg: 3.708 ± 0.071	4.258ValSer: 4.258 ± 0.07	4.628ValThr: 4.628 ± 0.088	4.51ValVal: 4.51 ± 0.073	0.704ValTrp: 0.704 ± 0.029	2.667ValTyr: 2.667 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.652TrpAla: 0.652 ± 0.031	0.161TrpCys: 0.161 ± 0.014	0.635TrpAsp: 0.635 ± 0.027	0.622TrpGlu: 0.622 ± 0.028	0.446TrpPhe: 0.446 ± 0.022	0.674TrpGly: 0.674 ± 0.029	0.236TrpHis: 0.236 ± 0.015	0.688TrpIle: 0.688 ± 0.03	0.723TrpLys: 0.723 ± 0.029	1.031TrpLeu: 1.031 ± 0.034	0.316TrpMet: 0.316 ± 0.019	0.617TrpAsn: 0.617 ± 0.031	0.275TrpPro: 0.275 ± 0.016	0.551TrpGln: 0.551 ± 0.027	0.467TrpArg: 0.467 ± 0.024	0.605TrpSer: 0.605 ± 0.032	0.583TrpThr: 0.583 ± 0.026	0.507TrpVal: 0.507 ± 0.024	0.166TrpTrp: 0.166 ± 0.011	0.468TrpTyr: 0.468 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.011TyrAla: 3.011 ± 0.051	0.586TyrCys: 0.586 ± 0.026	2.785TyrAsp: 2.785 ± 0.068	2.744TyrGlu: 2.744 ± 0.059	1.704TyrPhe: 1.704 ± 0.038	2.979TyrGly: 2.979 ± 0.071	1.033TyrHis: 1.033 ± 0.038	2.447TyrIle: 2.447 ± 0.049	1.508TyrLys: 1.508 ± 0.05	3.801TyrLeu: 3.801 ± 0.075	0.986TyrMet: 0.986 ± 0.033	1.651TyrAsn: 1.651 ± 0.05	1.618TyrPro: 1.618 ± 0.049	1.484TyrGln: 1.484 ± 0.048	2.47TyrArg: 2.47 ± 0.058	2.249TyrSer: 2.249 ± 0.053	2.254TyrThr: 2.254 ± 0.062	2.445TyrVal: 2.445 ± 0.056	0.423TyrTrp: 0.423 ± 0.023	1.883TyrTyr: 1.883 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2503 proteins (869147 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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