Salmonella phage 37
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N7CD05|A0A0N7CD05_9CAUD Uncharacterized protein OS=Salmonella phage 37 OX=1654890 GN=SP37_6 PE=4 SV=1
MM1 pKa = 7.66 FYY3 pKa = 10.29 TVLLNPDD10 pKa = 3.4 YY11 pKa = 11.35 EE12 pKa = 4.5 GATNVKK18 pKa = 8.42 TFYY21 pKa = 11.25 VEE23 pKa = 3.96 GATVEE28 pKa = 4.32 DD29 pKa = 4.71 AVTAAIDD36 pKa = 4.54 DD37 pKa = 4.16 INAHH41 pKa = 5.14 EE42 pKa = 4.22 VEE44 pKa = 4.58 IGGEE48 pKa = 3.87 PLARR52 pKa = 11.84 DD53 pKa = 3.66 QFSIIAVFPGYY64 pKa = 9.92 QPNVWEE70 pKa = 4.6 GG71 pKa = 3.07
Molecular weight: 7.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.834
IPC_protein 3.694
Toseland 3.528
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.452
Solomon 3.643
Lehninger 3.605
Nozaki 3.821
DTASelect 3.948
Thurlkill 3.592
EMBOSS 3.63
Sillero 3.821
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.795
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A0N7C9U5|A0A0N7C9U5_9CAUD Uncharacterized protein OS=Salmonella phage 37 OX=1654890 GN=SP37_20 PE=4 SV=1
MM1 pKa = 7.91 AEE3 pKa = 4.11 PIIRR7 pKa = 11.84 EE8 pKa = 4.09 SKK10 pKa = 9.83 VEE12 pKa = 4.01 KK13 pKa = 10.23 RR14 pKa = 11.84 CCEE17 pKa = 3.92 YY18 pKa = 11.12 AQGRR22 pKa = 11.84 GWWVSKK28 pKa = 8.52 FTAPGKK34 pKa = 10.2 KK35 pKa = 9.52 AVPDD39 pKa = 3.36 RR40 pKa = 11.84 VLIRR44 pKa = 11.84 GGIVLLSNSNARR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 TNGAAVSPPP67 pKa = 3.2
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.604
IPC_protein 10.233
Toseland 10.833
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.155
Grimsley 10.921
Solomon 11.038
Lehninger 11.008
Nozaki 10.833
DTASelect 10.57
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.833
Patrickios 10.95
IPC_peptide 11.052
IPC2_peptide 9.692
IPC2.peptide.svr19 8.657
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
105
0
105
18109
37
706
172.5
19.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.691 ± 0.396
1.121 ± 0.098
6.533 ± 0.231
5.92 ± 0.329
3.429 ± 0.146
7.118 ± 0.286
1.712 ± 0.13
5.395 ± 0.157
5.047 ± 0.257
7.322 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.17 ± 0.112
4.081 ± 0.176
4.721 ± 0.301
3.849 ± 0.299
6.577 ± 0.254
5.743 ± 0.186
6.361 ± 0.278
6.997 ± 0.28
1.751 ± 0.121
3.462 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here