Halovirus HHTV-1
Average proteome isoelectric point is 5.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4TM75|R4TM75_9VIRU Uncharacterized protein OS=Halovirus HHTV-1 OX=1273750 GN=12 PE=4 SV=1
MM1 pKa = 7.1 ATDD4 pKa = 3.8 RR5 pKa = 11.84 NTYY8 pKa = 8.71 PCRR11 pKa = 11.84 GCGKK15 pKa = 9.38 PFLEE19 pKa = 4.8 DD20 pKa = 3.76 GPRR23 pKa = 11.84 KK24 pKa = 9.62 AHH26 pKa = 6.86 EE27 pKa = 4.51 SSCPQAPDD35 pKa = 3.8 PEE37 pKa = 4.53 KK38 pKa = 11.13 ADD40 pKa = 3.58 ISDD43 pKa = 4.11 TEE45 pKa = 4.29 PPEE48 pKa = 5.62 APDD51 pKa = 3.7 STPEE55 pKa = 4.01 PQGEE59 pKa = 4.19 APEE62 pKa = 4.52 EE63 pKa = 3.94 PDD65 pKa = 3.2 RR66 pKa = 11.84 PARR69 pKa = 11.84 EE70 pKa = 4.2 PEE72 pKa = 4.24 VQQEE76 pKa = 4.22 PRR78 pKa = 11.84 SPADD82 pKa = 3.32 PHH84 pKa = 7.05 PDD86 pKa = 3.65 PEE88 pKa = 5.68 PEE90 pKa = 3.95 ADD92 pKa = 4.92 AGCSCCGADD101 pKa = 4.9 PGSLSINGMCYY112 pKa = 9.12 GCEE115 pKa = 4.07 LAGCDD120 pKa = 4.58 PDD122 pKa = 5.76 SLGCDD127 pKa = 3.58 HH128 pKa = 7.19 LPGGGG133 pKa = 3.27
Molecular weight: 13.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.825
IPC2_protein 4.024
IPC_protein 3.986
Toseland 3.795
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.859
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.253
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.101
Patrickios 0.807
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.987
Protein with the highest isoelectric point:
>tr|R4TGA6|R4TGA6_9VIRU Uncharacterized protein OS=Halovirus HHTV-1 OX=1273750 GN=10 PE=4 SV=1
MM1 pKa = 6.6 MLKK4 pKa = 10.48 RR5 pKa = 11.84 ITDD8 pKa = 4.42 WILGEE13 pKa = 4.11 KK14 pKa = 9.01 VANSEE19 pKa = 4.26 TRR21 pKa = 11.84 HH22 pKa = 4.53 QPAWFPSTDD31 pKa = 3.53 FDD33 pKa = 3.52 IRR35 pKa = 11.84 PFGEE39 pKa = 3.7 RR40 pKa = 11.84 LALFRR45 pKa = 11.84 LPPYY49 pKa = 8.99 KK50 pKa = 10.09 PPYY53 pKa = 8.99 RR54 pKa = 11.84 RR55 pKa = 11.84 HH56 pKa = 5.48 FRR58 pKa = 11.84 LKK60 pKa = 9.59 FWICFRR66 pKa = 11.84 QFHH69 pKa = 6.21 RR70 pKa = 11.84 AGFVEE75 pKa = 4.58 LDD77 pKa = 3.31 LDD79 pKa = 4.52 EE80 pKa = 4.74 RR81 pKa = 11.84 TLDD84 pKa = 3.52 LDD86 pKa = 4.33 LYY88 pKa = 10.83 RR89 pKa = 6.0
Molecular weight: 10.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 8.99
IPC_protein 9.209
Toseland 9.633
ProMoST 9.692
Dawson 9.897
Bjellqvist 9.648
Wikipedia 10.101
Rodwell 10.058
Grimsley 9.984
Solomon 9.999
Lehninger 9.955
Nozaki 9.706
DTASelect 9.619
Thurlkill 9.736
EMBOSS 10.058
Sillero 9.838
Patrickios 7.644
IPC_peptide 9.984
IPC2_peptide 8.463
IPC2.peptide.svr19 8.011
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
15566
37
1100
210.4
23.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.223 ± 0.373
0.784 ± 0.15
9.553 ± 0.316
8.422 ± 0.471
3.386 ± 0.17
8.641 ± 0.247
2.242 ± 0.215
5.139 ± 0.241
2.64 ± 0.162
6.964 ± 0.392
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.422 ± 0.159
3.559 ± 0.211
5.223 ± 0.299
4.998 ± 0.198
5.261 ± 0.287
6.238 ± 0.28
5.808 ± 0.298
6.662 ± 0.201
1.523 ± 0.135
2.313 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here