Amino acid dipepetide frequency for Candidatus Synechococcus spongiarum LMB bulk10D

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.875AlaAla: 12.875 ± 0.474
1.568AlaCys: 1.568 ± 0.113
4.967AlaAsp: 4.967 ± 0.199
5.556AlaGlu: 5.556 ± 0.203
2.902AlaPhe: 2.902 ± 0.149
8.257AlaGly: 8.257 ± 0.261
2.272AlaHis: 2.272 ± 0.138
4.095AlaIle: 4.095 ± 0.175
2.493AlaLys: 2.493 ± 0.154
12.507AlaLeu: 12.507 ± 0.372
2.875AlaMet: 2.875 ± 0.137
2.52AlaAsn: 2.52 ± 0.123
4.564AlaPro: 4.564 ± 0.217
4.189AlaGln: 4.189 ± 0.183
7.205AlaArg: 7.205 ± 0.236
5.905AlaSer: 5.905 ± 0.196
5.597AlaThr: 5.597 ± 0.243
8.82AlaVal: 8.82 ± 0.309
1.401AlaTrp: 1.401 ± 0.102
1.971AlaTyr: 1.971 ± 0.105
0.0AlaXaa: 0.0 ± 0.0
Cys
0.878CysAla: 0.878 ± 0.078
0.335CysCys: 0.335 ± 0.049
0.677CysAsp: 0.677 ± 0.072
0.436CysGlu: 0.436 ± 0.061
0.516CysPhe: 0.516 ± 0.067
1.401CysGly: 1.401 ± 0.102
0.456CysHis: 0.456 ± 0.054
0.576CysIle: 0.576 ± 0.062
0.402CysLys: 0.402 ± 0.057
1.454CysLeu: 1.454 ± 0.099
0.235CysMet: 0.235 ± 0.039
0.436CysAsn: 0.436 ± 0.059
0.851CysPro: 0.851 ± 0.076
0.556CysGln: 0.556 ± 0.061
1.327CysArg: 1.327 ± 0.106
0.777CysSer: 0.777 ± 0.065
0.677CysThr: 0.677 ± 0.072
0.784CysVal: 0.784 ± 0.075
0.322CysTrp: 0.322 ± 0.053
0.362CysTyr: 0.362 ± 0.047
0.0CysXaa: 0.0 ± 0.0
Asp
5.06AspAla: 5.06 ± 0.182
0.623AspCys: 0.623 ± 0.075
2.574AspAsp: 2.574 ± 0.144
2.567AspGlu: 2.567 ± 0.132
1.743AspPhe: 1.743 ± 0.121
4.551AspGly: 4.551 ± 0.196
1.649AspHis: 1.649 ± 0.119
2.004AspIle: 2.004 ± 0.138
1.501AspLys: 1.501 ± 0.122
6.495AspLeu: 6.495 ± 0.219
0.905AspMet: 0.905 ± 0.071
1.515AspAsn: 1.515 ± 0.119
3.706AspPro: 3.706 ± 0.176
2.406AspGln: 2.406 ± 0.134
3.747AspArg: 3.747 ± 0.167
2.782AspSer: 2.782 ± 0.149
2.172AspThr: 2.172 ± 0.13
3.901AspVal: 3.901 ± 0.157
0.905AspTrp: 0.905 ± 0.095
1.394AspTyr: 1.394 ± 0.099
0.0AspXaa: 0.0 ± 0.0
Glu
6.18GluAla: 6.18 ± 0.243
0.395GluCys: 0.395 ± 0.05
3.184GluAsp: 3.184 ± 0.138
3.499GluGlu: 3.499 ± 0.193
1.488GluPhe: 1.488 ± 0.097
3.934GluGly: 3.934 ± 0.156
1.139GluHis: 1.139 ± 0.093
2.319GluIle: 2.319 ± 0.149
1.836GluLys: 1.836 ± 0.128
6.233GluLeu: 6.233 ± 0.195
1.26GluMet: 1.26 ± 0.107
1.461GluAsn: 1.461 ± 0.103
2.641GluPro: 2.641 ± 0.138
3.13GluGln: 3.13 ± 0.141
5.121GluArg: 5.121 ± 0.206
2.601GluSer: 2.601 ± 0.115
2.983GluThr: 2.983 ± 0.144
3.532GluVal: 3.532 ± 0.17
0.523GluTrp: 0.523 ± 0.068
0.757GluTyr: 0.757 ± 0.074
0.0GluXaa: 0.0 ± 0.0
Phe
3.184PheAla: 3.184 ± 0.144
0.617PheCys: 0.617 ± 0.058
1.903PheAsp: 1.903 ± 0.123
1.421PheGlu: 1.421 ± 0.1
1.166PhePhe: 1.166 ± 0.103
2.822PheGly: 2.822 ± 0.161
0.871PheHis: 0.871 ± 0.085
1.18PheIle: 1.18 ± 0.085
0.851PheLys: 0.851 ± 0.088
3.559PheLeu: 3.559 ± 0.181
0.496PheMet: 0.496 ± 0.056
1.059PheAsn: 1.059 ± 0.083
1.521PhePro: 1.521 ± 0.091
1.287PheGln: 1.287 ± 0.084
2.326PheArg: 2.326 ± 0.122
2.252PheSer: 2.252 ± 0.145
1.877PheThr: 1.877 ± 0.112
2.118PheVal: 2.118 ± 0.118
0.556PheTrp: 0.556 ± 0.066
0.918PheTyr: 0.918 ± 0.085
0.0PheXaa: 0.0 ± 0.0
Gly
7.319GlyAla: 7.319 ± 0.256
1.414GlyCys: 1.414 ± 0.113
4.276GlyAsp: 4.276 ± 0.155
4.471GlyGlu: 4.471 ± 0.177
3.224GlyPhe: 3.224 ± 0.161
6.441GlyGly: 6.441 ± 0.246
2.098GlyHis: 2.098 ± 0.13
3.847GlyIle: 3.847 ± 0.164
2.882GlyLys: 2.882 ± 0.179
9.772GlyLeu: 9.772 ± 0.251
2.071GlyMet: 2.071 ± 0.125
2.286GlyAsn: 2.286 ± 0.158
3.606GlyPro: 3.606 ± 0.194
4.028GlyGln: 4.028 ± 0.21
5.71GlyArg: 5.71 ± 0.21
5.127GlySer: 5.127 ± 0.18
4.323GlyThr: 4.323 ± 0.269
6.307GlyVal: 6.307 ± 0.264
1.421GlyTrp: 1.421 ± 0.101
1.897GlyTyr: 1.897 ± 0.099
0.0GlyXaa: 0.0 ± 0.0
His
1.903HisAla: 1.903 ± 0.119
0.449HisCys: 0.449 ± 0.06
1.139HisAsp: 1.139 ± 0.088
0.885HisGlu: 0.885 ± 0.08
0.771HisPhe: 0.771 ± 0.067
2.748HisGly: 2.748 ± 0.115
1.025HisHis: 1.025 ± 0.111
0.958HisIle: 0.958 ± 0.099
0.576HisLys: 0.576 ± 0.054
3.036HisLeu: 3.036 ± 0.163
0.469HisMet: 0.469 ± 0.055
0.664HisAsn: 0.664 ± 0.071
1.776HisPro: 1.776 ± 0.116
1.394HisGln: 1.394 ± 0.107
2.118HisArg: 2.118 ± 0.134
1.361HisSer: 1.361 ± 0.094
0.985HisThr: 0.985 ± 0.08
1.823HisVal: 1.823 ± 0.137
0.483HisTrp: 0.483 ± 0.059
0.664HisTyr: 0.664 ± 0.069
0.0HisXaa: 0.0 ± 0.0
Ile
3.981IleAla: 3.981 ± 0.17
0.55IleCys: 0.55 ± 0.076
2.192IleAsp: 2.192 ± 0.133
2.017IleGlu: 2.017 ± 0.135
1.294IlePhe: 1.294 ± 0.104
3.653IleGly: 3.653 ± 0.162
1.173IleHis: 1.173 ± 0.094
1.495IleIle: 1.495 ± 0.142
1.401IleLys: 1.401 ± 0.126
3.934IleLeu: 3.934 ± 0.201
0.617IleMet: 0.617 ± 0.08
1.575IleAsn: 1.575 ± 0.108
2.547IlePro: 2.547 ± 0.132
1.595IleGln: 1.595 ± 0.094
3.19IleArg: 3.19 ± 0.163
2.688IleSer: 2.688 ± 0.148
2.594IleThr: 2.594 ± 0.146
2.48IleVal: 2.48 ± 0.135
0.503IleTrp: 0.503 ± 0.057
0.932IleTyr: 0.932 ± 0.09
0.0IleXaa: 0.0 ± 0.0
Lys
3.412LysAla: 3.412 ± 0.176
0.241LysCys: 0.241 ± 0.041
1.729LysAsp: 1.729 ± 0.117
1.595LysGlu: 1.595 ± 0.119
0.751LysPhe: 0.751 ± 0.079
2.219LysGly: 2.219 ± 0.138
0.63LysHis: 0.63 ± 0.075
1.213LysIle: 1.213 ± 0.113
1.099LysLys: 1.099 ± 0.116
3.204LysLeu: 3.204 ± 0.162
0.55LysMet: 0.55 ± 0.061
0.757LysAsn: 0.757 ± 0.087
1.689LysPro: 1.689 ± 0.108
1.314LysGln: 1.314 ± 0.106
2.105LysArg: 2.105 ± 0.14
1.521LysSer: 1.521 ± 0.099
1.964LysThr: 1.964 ± 0.126
2.239LysVal: 2.239 ± 0.135
0.268LysTrp: 0.268 ± 0.043
0.523LysTyr: 0.523 ± 0.063
0.0LysXaa: 0.0 ± 0.0
Leu
13.15LeuAla: 13.15 ± 0.364
1.448LeuCys: 1.448 ± 0.095
6.528LeuAsp: 6.528 ± 0.209
7.467LeuGlu: 7.467 ± 0.22
3.231LeuPhe: 3.231 ± 0.166
8.954LeuGly: 8.954 ± 0.256
2.869LeuHis: 2.869 ± 0.132
3.948LeuIle: 3.948 ± 0.185
3.519LeuLys: 3.519 ± 0.15
13.68LeuLeu: 13.68 ± 0.504
2.245LeuMet: 2.245 ± 0.126
3.076LeuAsn: 3.076 ± 0.132
6.629LeuPro: 6.629 ± 0.22
5.744LeuGln: 5.744 ± 0.194
8.586LeuArg: 8.586 ± 0.241
6.756LeuSer: 6.756 ± 0.203
5.918LeuThr: 5.918 ± 0.189
9.062LeuVal: 9.062 ± 0.34
1.649LeuTrp: 1.649 ± 0.116
1.964LeuTyr: 1.964 ± 0.112
0.0LeuXaa: 0.0 ± 0.0
Met
3.525MetAla: 3.525 ± 0.153
0.101MetCys: 0.101 ± 0.026
1.18MetAsp: 1.18 ± 0.084
1.495MetGlu: 1.495 ± 0.096
0.576MetPhe: 0.576 ± 0.069
1.79MetGly: 1.79 ± 0.088
0.335MetHis: 0.335 ± 0.05
0.798MetIle: 0.798 ± 0.078
0.724MetLys: 0.724 ± 0.08
1.796MetLeu: 1.796 ± 0.123
0.395MetMet: 0.395 ± 0.051
0.483MetAsn: 0.483 ± 0.056
1.193MetPro: 1.193 ± 0.095
0.643MetGln: 0.643 ± 0.069
1.193MetArg: 1.193 ± 0.09
1.2MetSer: 1.2 ± 0.081
1.387MetThr: 1.387 ± 0.097
1.984MetVal: 1.984 ± 0.131
0.121MetTrp: 0.121 ± 0.032
0.295MetTyr: 0.295 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
2.386AsnAla: 2.386 ± 0.132
0.402AsnCys: 0.402 ± 0.058
1.287AsnAsp: 1.287 ± 0.096
1.099AsnGlu: 1.099 ± 0.102
1.039AsnPhe: 1.039 ± 0.096
2.319AsnGly: 2.319 ± 0.153
0.664AsnHis: 0.664 ± 0.06
1.012AsnIle: 1.012 ± 0.093
0.865AsnLys: 0.865 ± 0.077
3.11AsnLeu: 3.11 ± 0.131
0.529AsnMet: 0.529 ± 0.051
0.858AsnAsn: 0.858 ± 0.098
2.158AsnPro: 2.158 ± 0.122
1.22AsnGln: 1.22 ± 0.103
2.265AsnArg: 2.265 ± 0.119
1.702AsnSer: 1.702 ± 0.116
1.562AsnThr: 1.562 ± 0.099
1.883AsnVal: 1.883 ± 0.107
0.489AsnTrp: 0.489 ± 0.059
0.724AsnTyr: 0.724 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
5.181ProAla: 5.181 ± 0.201
0.637ProCys: 0.637 ± 0.073
3.512ProAsp: 3.512 ± 0.162
3.914ProGlu: 3.914 ± 0.185
1.723ProPhe: 1.723 ± 0.121
5.134ProGly: 5.134 ± 0.213
1.307ProHis: 1.307 ± 0.091
1.957ProIle: 1.957 ± 0.112
1.26ProLys: 1.26 ± 0.092
6.408ProLeu: 6.408 ± 0.22
1.367ProMet: 1.367 ± 0.092
1.528ProAsn: 1.528 ± 0.097
3.318ProPro: 3.318 ± 0.186
2.212ProGln: 2.212 ± 0.126
3.485ProArg: 3.485 ± 0.169
3.412ProSer: 3.412 ± 0.198
3.063ProThr: 3.063 ± 0.161
4.35ProVal: 4.35 ± 0.183
1.079ProTrp: 1.079 ± 0.078
1.26ProTyr: 1.26 ± 0.102
0.0ProXaa: 0.0 ± 0.0
Gln
5.208GlnAla: 5.208 ± 0.158
0.516GlnCys: 0.516 ± 0.059
2.406GlnAsp: 2.406 ± 0.141
2.748GlnGlu: 2.748 ± 0.134
1.153GlnPhe: 1.153 ± 0.088
3.733GlnGly: 3.733 ± 0.169
1.2GlnHis: 1.2 ± 0.091
1.334GlnIle: 1.334 ± 0.105
1.146GlnLys: 1.146 ± 0.094
5.932GlnLeu: 5.932 ± 0.213
0.71GlnMet: 0.71 ± 0.062
0.965GlnAsn: 0.965 ± 0.079
3.345GlnPro: 3.345 ± 0.174
3.016GlnGln: 3.016 ± 0.138
4.564GlnArg: 4.564 ± 0.202
1.937GlnSer: 1.937 ± 0.114
1.843GlnThr: 1.843 ± 0.122
3.908GlnVal: 3.908 ± 0.157
0.69GlnTrp: 0.69 ± 0.062
0.677GlnTyr: 0.677 ± 0.075
0.0GlnXaa: 0.0 ± 0.0
Arg
5.449ArgAla: 5.449 ± 0.183
1.24ArgCys: 1.24 ± 0.095
3.525ArgAsp: 3.525 ± 0.156
3.894ArgGlu: 3.894 ± 0.171
3.123ArgPhe: 3.123 ± 0.158
4.792ArgGly: 4.792 ± 0.207
2.621ArgHis: 2.621 ± 0.145
3.981ArgIle: 3.981 ± 0.17
2.567ArgLys: 2.567 ± 0.143
9.672ArgLeu: 9.672 ± 0.278
1.501ArgMet: 1.501 ± 0.101
2.111ArgAsn: 2.111 ± 0.111
3.546ArgPro: 3.546 ± 0.169
5.349ArgGln: 5.349 ± 0.224
6.528ArgArg: 6.528 ± 0.288
4.517ArgSer: 4.517 ± 0.196
3.318ArgThr: 3.318 ± 0.156
4.712ArgVal: 4.712 ± 0.2
1.408ArgTrp: 1.408 ± 0.106
1.977ArgTyr: 1.977 ± 0.128
0.0ArgXaa: 0.0 ± 0.0
Ser
5.147SerAla: 5.147 ± 0.24
0.717SerCys: 0.717 ± 0.067
2.58SerAsp: 2.58 ± 0.158
2.54SerGlu: 2.54 ± 0.14
1.89SerPhe: 1.89 ± 0.115
5.67SerGly: 5.67 ± 0.225
1.562SerHis: 1.562 ± 0.11
2.178SerIle: 2.178 ± 0.121
1.642SerLys: 1.642 ± 0.107
6.615SerLeu: 6.615 ± 0.232
1.354SerMet: 1.354 ± 0.103
1.736SerAsn: 1.736 ± 0.112
3.566SerPro: 3.566 ± 0.177
2.426SerGln: 2.426 ± 0.125
5.241SerArg: 5.241 ± 0.217
3.908SerSer: 3.908 ± 0.193
3.465SerThr: 3.465 ± 0.167
3.827SerVal: 3.827 ± 0.17
1.019SerTrp: 1.019 ± 0.089
1.233SerTyr: 1.233 ± 0.09
0.0SerXaa: 0.0 ± 0.0
Thr
6.414ThrAla: 6.414 ± 0.24
0.724ThrCys: 0.724 ± 0.071
2.567ThrAsp: 2.567 ± 0.123
2.326ThrGlu: 2.326 ± 0.135
1.776ThrPhe: 1.776 ± 0.136
5.054ThrGly: 5.054 ± 0.279
1.046ThrHis: 1.046 ± 0.074
2.554ThrIle: 2.554 ± 0.133
1.106ThrLys: 1.106 ± 0.087
5.563ThrLeu: 5.563 ± 0.236
1.28ThrMet: 1.28 ± 0.096
1.314ThrAsn: 1.314 ± 0.091
3.499ThrPro: 3.499 ± 0.187
1.575ThrGln: 1.575 ± 0.097
3.07ThrArg: 3.07 ± 0.15
3.391ThrSer: 3.391 ± 0.163
3.934ThrThr: 3.934 ± 0.265
5.027ThrVal: 5.027 ± 0.21
0.664ThrTrp: 0.664 ± 0.076
1.22ThrTyr: 1.22 ± 0.091
0.0ThrXaa: 0.0 ± 0.0
Val
8.412ValAla: 8.412 ± 0.265
0.918ValCys: 0.918 ± 0.074
4.001ValAsp: 4.001 ± 0.178
4.571ValGlu: 4.571 ± 0.179
2.574ValPhe: 2.574 ± 0.138
5.731ValGly: 5.731 ± 0.231
1.475ValHis: 1.475 ± 0.102
3.338ValIle: 3.338 ± 0.163
2.245ValLys: 2.245 ± 0.131
8.76ValLeu: 8.76 ± 0.296
1.588ValMet: 1.588 ± 0.093
2.192ValAsn: 2.192 ± 0.114
3.82ValPro: 3.82 ± 0.17
3.09ValGln: 3.09 ± 0.148
4.946ValArg: 4.946 ± 0.186
4.223ValSer: 4.223 ± 0.189
4.732ValThr: 4.732 ± 0.232
6.81ValVal: 6.81 ± 0.253
1.072ValTrp: 1.072 ± 0.082
1.32ValTyr: 1.32 ± 0.095
0.0ValXaa: 0.0 ± 0.0
Trp
1.099TrpAla: 1.099 ± 0.1
0.174TrpCys: 0.174 ± 0.035
0.737TrpAsp: 0.737 ± 0.082
0.65TrpGlu: 0.65 ± 0.074
0.469TrpPhe: 0.469 ± 0.061
0.958TrpGly: 0.958 ± 0.081
0.389TrpHis: 0.389 ± 0.056
0.724TrpIle: 0.724 ± 0.069
0.422TrpLys: 0.422 ± 0.056
2.379TrpLeu: 2.379 ± 0.159
0.275TrpMet: 0.275 ± 0.044
0.382TrpAsn: 0.382 ± 0.046
0.932TrpPro: 0.932 ± 0.087
1.046TrpGln: 1.046 ± 0.098
1.233TrpArg: 1.233 ± 0.097
1.066TrpSer: 1.066 ± 0.094
0.731TrpThr: 0.731 ± 0.08
0.945TrpVal: 0.945 ± 0.094
0.268TrpTrp: 0.268 ± 0.046
0.302TrpTyr: 0.302 ± 0.042
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.776TyrAla: 1.776 ± 0.132
0.389TyrCys: 0.389 ± 0.051
1.233TyrAsp: 1.233 ± 0.086
1.066TyrGlu: 1.066 ± 0.084
0.576TyrPhe: 0.576 ± 0.064
2.359TyrGly: 2.359 ± 0.121
0.449TyrHis: 0.449 ± 0.061
0.898TyrIle: 0.898 ± 0.089
0.483TyrLys: 0.483 ± 0.066
2.312TyrLeu: 2.312 ± 0.133
0.483TyrMet: 0.483 ± 0.059
0.657TyrAsn: 0.657 ± 0.059
1.2TyrPro: 1.2 ± 0.089
0.811TyrGln: 0.811 ± 0.077
1.877TyrArg: 1.877 ± 0.121
1.193TyrSer: 1.193 ± 0.097
0.979TyrThr: 0.979 ± 0.1
1.307TyrVal: 1.307 ± 0.111
0.342TyrTrp: 0.342 ± 0.049
0.436TyrTyr: 0.436 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 532 proteins (149200 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski