Oceanicoccus sagamiensis
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3870 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X9NII4|A0A1X9NII4_9GAMM STN domain-containing protein OS=Oceanicoccus sagamiensis OX=716816 GN=BST96_06500 PE=3 SV=1
MM1 pKa = 7.3 TSNPILLAAFSLALAQPALSQVNMALLGDD30 pKa = 4.62 SIIDD34 pKa = 4.16 DD35 pKa = 3.88 YY36 pKa = 11.65 LGPSNSIGTNTNLAAGSFGQILADD60 pKa = 3.73 TRR62 pKa = 11.84 GADD65 pKa = 3.52 INFGAYY71 pKa = 8.51 KK72 pKa = 10.64 APTANTADD80 pKa = 3.28 AWDD83 pKa = 4.11 SIRR86 pKa = 11.84 NFGYY90 pKa = 10.05 EE91 pKa = 4.36 YY92 pKa = 11.14 NWATAGGTASAQEE105 pKa = 4.29 LNLDD109 pKa = 4.02 FNGPDD114 pKa = 3.23 APGYY118 pKa = 9.49 AQIPIASNLAAQVAGLAPSISSGDD142 pKa = 3.0 VDD144 pKa = 3.63 TAVISVGPNDD154 pKa = 4.02 FFYY157 pKa = 10.99 HH158 pKa = 5.98 SLVIDD163 pKa = 3.72 TASGGFYY170 pKa = 9.43 PAPDD174 pKa = 3.7 GQLDD178 pKa = 3.53 QTFTNLVADD187 pKa = 5.14 SILEE191 pKa = 4.85 GIDD194 pKa = 3.2 TLQAAGDD201 pKa = 3.6 VDD203 pKa = 4.73 IILGLLAKK211 pKa = 10.37 RR212 pKa = 11.84 PGLSEE217 pKa = 4.28 EE218 pKa = 4.31 EE219 pKa = 3.89 NTAIDD224 pKa = 3.85 TVNNRR229 pKa = 11.84 LLTEE233 pKa = 4.05 ATEE236 pKa = 4.19 KK237 pKa = 11.02 GVVVLDD243 pKa = 3.88 FMEE246 pKa = 4.21 WTISGEE252 pKa = 4.15 NVDD255 pKa = 5.88 PITQDD260 pKa = 3.05 VTIGDD265 pKa = 3.84 VVVEE269 pKa = 4.15 YY270 pKa = 11.06 GSVASISDD278 pKa = 3.48 MSTEE282 pKa = 4.2 GDD284 pKa = 3.53 GAYY287 pKa = 8.29 CTYY290 pKa = 10.97 EE291 pKa = 4.12 GLCPLDD297 pKa = 3.35 SHH299 pKa = 6.69 AFSYY303 pKa = 10.47 LAEE306 pKa = 5.25 DD307 pKa = 3.95 GRR309 pKa = 11.84 HH310 pKa = 6.15 LNTLMQGMLANEE322 pKa = 4.7 ILTAMNTHH330 pKa = 6.91 FDD332 pKa = 3.43 HH333 pKa = 7.98 DD334 pKa = 4.19 IDD336 pKa = 4.54 LLSDD340 pKa = 3.85 SEE342 pKa = 4.41 LLGLVGVSEE351 pKa = 4.74 VPVPAAAWLFMSALLGLVGIKK372 pKa = 9.89 RR373 pKa = 11.84 RR374 pKa = 11.84 NN375 pKa = 3.13
Molecular weight: 39.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 1.202
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A1X9NGP8|A0A1X9NGP8_9GAMM Selenide water dikinase SelD OS=Oceanicoccus sagamiensis OX=716816 GN=BST96_20065 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.43 NGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3870
0
3870
1275952
25
14664
329.7
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.43 ± 0.048
0.965 ± 0.017
6.162 ± 0.068
6.139 ± 0.044
3.891 ± 0.028
7.326 ± 0.065
2.007 ± 0.026
6.225 ± 0.035
4.517 ± 0.053
10.04 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.027
4.114 ± 0.047
4.175 ± 0.038
4.571 ± 0.039
4.627 ± 0.05
6.679 ± 0.039
5.445 ± 0.063
6.839 ± 0.036
1.245 ± 0.018
3.086 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here