bacterium B17

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 777 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DEM4|A0A202DEM4_9BACT 23S rRNA (Guanosine(2251)-2'-O)-methyltransferase RlmB OS=bacterium B17 OX=1932704 GN=BVX94_00425 PE=4 SV=1
MM1 pKa = 7.71LLCCLPVSVLAEE13 pKa = 3.86IDD15 pKa = 4.42LIADD19 pKa = 4.2IVPGAIIANYY29 pKa = 9.47NADD32 pKa = 3.62AFSLEE37 pKa = 3.94GSQGKK42 pKa = 7.8EE43 pKa = 3.85TMSLVSSVPSAEE55 pKa = 5.36IGIAWQQPEE64 pKa = 4.06GSIDD68 pKa = 3.45LKK70 pKa = 11.25AGVGALLNSRR80 pKa = 11.84LMSYY84 pKa = 9.6TMAVNLGLTLEE95 pKa = 4.23MQPSIMIGPHH105 pKa = 6.49IGMIYY110 pKa = 9.75YY111 pKa = 9.83SEE113 pKa = 4.54PEE115 pKa = 3.39WWGDD119 pKa = 3.54GEE121 pKa = 4.56ITFSDD126 pKa = 3.54SSGVTLGMHH135 pKa = 6.35MNLGDD140 pKa = 3.37KK141 pKa = 9.29VAYY144 pKa = 8.25MFSVDD149 pKa = 3.56YY150 pKa = 10.59YY151 pKa = 11.13SAVFDD156 pKa = 4.24IEE158 pKa = 4.89SIGAGWTTDD167 pKa = 3.07QDD169 pKa = 3.95EE170 pKa = 5.24LDD172 pKa = 3.64MSGLAFRR179 pKa = 11.84FGIRR183 pKa = 11.84AQFF186 pKa = 3.34

Molecular weight: 20.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DF94|A0A202DF94_9BACT Glucose-6-phosphate isomerase (Fragment) OS=bacterium B17 OX=1932704 GN=BVX94_00015 PE=3 SV=1
MM1 pKa = 7.19KK2 pKa = 10.1RR3 pKa = 11.84VYY5 pKa = 10.31QPSNKK10 pKa = 8.93KK11 pKa = 8.46RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 8.03IGFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84GGRR28 pKa = 11.84AIIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.81GRR39 pKa = 11.84AEE41 pKa = 3.73LTAA44 pKa = 4.95

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

777

0

777

242243

18

1960

311.8

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.043 ± 0.101

1.357 ± 0.039

6.154 ± 0.093

6.721 ± 0.101

4.026 ± 0.066

7.42 ± 0.107

2.02 ± 0.035

6.429 ± 0.064

6.723 ± 0.15

8.827 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.051

4.072 ± 0.076

4.099 ± 0.056

2.883 ± 0.057

4.817 ± 0.086

6.697 ± 0.093

5.208 ± 0.088

6.999 ± 0.076

1.451 ± 0.043

3.282 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski