Amino acid dipepetide frequency for bacterium B17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.422AlaAla: 7.422 ± 0.233
1.185AlaCys: 1.185 ± 0.086
4.669AlaAsp: 4.669 ± 0.135
6.172AlaGlu: 6.172 ± 0.181
3.228AlaPhe: 3.228 ± 0.116
6.539AlaGly: 6.539 ± 0.214
1.379AlaHis: 1.379 ± 0.08
4.884AlaIle: 4.884 ± 0.181
4.817AlaLys: 4.817 ± 0.179
7.216AlaLeu: 7.216 ± 0.203
2.386AlaMet: 2.386 ± 0.111
2.778AlaAsn: 2.778 ± 0.107
2.811AlaPro: 2.811 ± 0.11
2.266AlaGln: 2.266 ± 0.108
3.831AlaArg: 3.831 ± 0.141
5.177AlaSer: 5.177 ± 0.16
3.885AlaThr: 3.885 ± 0.141
6.316AlaVal: 6.316 ± 0.22
1.11AlaTrp: 1.11 ± 0.078
2.357AlaTyr: 2.357 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
1.032CysAla: 1.032 ± 0.067
0.206CysCys: 0.206 ± 0.032
0.817CysAsp: 0.817 ± 0.066
0.768CysGlu: 0.768 ± 0.065
0.66CysPhe: 0.66 ± 0.055
1.362CysGly: 1.362 ± 0.088
0.367CysHis: 0.367 ± 0.062
0.966CysIle: 0.966 ± 0.069
0.76CysLys: 0.76 ± 0.059
1.123CysLeu: 1.123 ± 0.077
0.359CysMet: 0.359 ± 0.04
0.429CysAsn: 0.429 ± 0.039
0.566CysPro: 0.566 ± 0.053
0.339CysGln: 0.339 ± 0.034
0.747CysArg: 0.747 ± 0.071
0.925CysSer: 0.925 ± 0.057
0.644CysThr: 0.644 ± 0.047
0.912CysVal: 0.912 ± 0.06
0.153CysTrp: 0.153 ± 0.024
0.438CysTyr: 0.438 ± 0.04
0.0CysXaa: 0.0 ± 0.0
Asp
4.714AspAla: 4.714 ± 0.162
0.768AspCys: 0.768 ± 0.06
3.657AspAsp: 3.657 ± 0.148
4.169AspGlu: 4.169 ± 0.146
2.774AspPhe: 2.774 ± 0.133
5.036AspGly: 5.036 ± 0.243
1.247AspHis: 1.247 ± 0.073
4.211AspIle: 4.211 ± 0.156
4.054AspLys: 4.054 ± 0.13
5.614AspLeu: 5.614 ± 0.141
1.631AspMet: 1.631 ± 0.08
2.39AspAsn: 2.39 ± 0.112
2.663AspPro: 2.663 ± 0.107
1.54AspGln: 1.54 ± 0.08
2.415AspArg: 2.415 ± 0.11
3.843AspSer: 3.843 ± 0.176
2.737AspThr: 2.737 ± 0.125
4.595AspVal: 4.595 ± 0.177
1.086AspTrp: 1.086 ± 0.078
2.398AspTyr: 2.398 ± 0.119
0.0AspXaa: 0.0 ± 0.0
Glu
5.288GluAla: 5.288 ± 0.157
0.755GluCys: 0.755 ± 0.06
3.769GluAsp: 3.769 ± 0.115
5.065GluGlu: 5.065 ± 0.206
2.266GluPhe: 2.266 ± 0.093
4.314GluGly: 4.314 ± 0.151
1.309GluHis: 1.309 ± 0.075
4.747GluIle: 4.747 ± 0.169
5.49GluLys: 5.49 ± 0.198
6.271GluLeu: 6.271 ± 0.283
1.973GluMet: 1.973 ± 0.106
3.034GluAsn: 3.034 ± 0.122
2.254GluPro: 2.254 ± 0.098
2.188GluGln: 2.188 ± 0.105
3.558GluArg: 3.558 ± 0.131
3.922GluSer: 3.922 ± 0.157
3.455GluThr: 3.455 ± 0.143
4.343GluVal: 4.343 ± 0.157
0.999GluTrp: 0.999 ± 0.065
2.204GluTyr: 2.204 ± 0.092
0.0GluXaa: 0.0 ± 0.0
Phe
3.084PheAla: 3.084 ± 0.144
0.66PheCys: 0.66 ± 0.058
2.646PheAsp: 2.646 ± 0.124
2.308PheGlu: 2.308 ± 0.101
1.911PhePhe: 1.911 ± 0.095
2.923PheGly: 2.923 ± 0.117
0.739PheHis: 0.739 ± 0.065
2.403PheIle: 2.403 ± 0.125
2.27PheLys: 2.27 ± 0.091
3.624PheLeu: 3.624 ± 0.138
1.205PheMet: 1.205 ± 0.075
1.779PheAsn: 1.779 ± 0.089
1.726PhePro: 1.726 ± 0.091
1.123PheGln: 1.123 ± 0.069
1.92PheArg: 1.92 ± 0.09
3.166PheSer: 3.166 ± 0.113
2.089PheThr: 2.089 ± 0.09
2.819PheVal: 2.819 ± 0.115
0.689PheTrp: 0.689 ± 0.052
1.172PheTyr: 1.172 ± 0.073
0.0PheXaa: 0.0 ± 0.0
Gly
5.416GlyAla: 5.416 ± 0.204
1.21GlyCys: 1.21 ± 0.085
4.582GlyAsp: 4.582 ± 0.232
4.343GlyGlu: 4.343 ± 0.138
3.232GlyPhe: 3.232 ± 0.12
5.528GlyGly: 5.528 ± 0.224
1.482GlyHis: 1.482 ± 0.086
4.933GlyIle: 4.933 ± 0.162
5.858GlyLys: 5.858 ± 0.184
6.176GlyLeu: 6.176 ± 0.201
2.345GlyMet: 2.345 ± 0.119
3.063GlyAsn: 3.063 ± 0.143
2.25GlyPro: 2.25 ± 0.104
1.915GlyGln: 1.915 ± 0.087
3.463GlyArg: 3.463 ± 0.133
4.978GlySer: 4.978 ± 0.161
4.363GlyThr: 4.363 ± 0.202
5.135GlyVal: 5.135 ± 0.181
1.309GlyTrp: 1.309 ± 0.119
2.621GlyTyr: 2.621 ± 0.119
0.0GlyXaa: 0.0 ± 0.0
His
1.614HisAla: 1.614 ± 0.081
0.38HisCys: 0.38 ± 0.046
1.251HisAsp: 1.251 ± 0.079
1.197HisGlu: 1.197 ± 0.061
0.929HisPhe: 0.929 ± 0.066
1.655HisGly: 1.655 ± 0.087
0.52HisHis: 0.52 ± 0.044
1.193HisIle: 1.193 ± 0.073
1.156HisLys: 1.156 ± 0.066
1.899HisLeu: 1.899 ± 0.094
0.516HisMet: 0.516 ± 0.046
0.867HisAsn: 0.867 ± 0.053
1.098HisPro: 1.098 ± 0.074
0.57HisGln: 0.57 ± 0.051
0.995HisArg: 0.995 ± 0.063
1.288HisSer: 1.288 ± 0.079
0.937HisThr: 0.937 ± 0.059
1.205HisVal: 1.205 ± 0.084
0.293HisTrp: 0.293 ± 0.04
0.636HisTyr: 0.636 ± 0.048
0.0HisXaa: 0.0 ± 0.0
Ile
5.346IleAla: 5.346 ± 0.171
0.925IleCys: 0.925 ± 0.068
4.24IleAsp: 4.24 ± 0.14
4.363IleGlu: 4.363 ± 0.159
2.097IlePhe: 2.097 ± 0.105
4.392IleGly: 4.392 ± 0.131
1.371IleHis: 1.371 ± 0.085
4.198IleIle: 4.198 ± 0.172
4.227IleLys: 4.227 ± 0.156
5.68IleLeu: 5.68 ± 0.201
1.622IleMet: 1.622 ± 0.092
2.605IleAsn: 2.605 ± 0.118
2.72IlePro: 2.72 ± 0.098
1.924IleGln: 1.924 ± 0.092
2.807IleArg: 2.807 ± 0.122
4.739IleSer: 4.739 ± 0.135
3.645IleThr: 3.645 ± 0.124
4.702IleVal: 4.702 ± 0.149
0.722IleTrp: 0.722 ± 0.052
1.961IleTyr: 1.961 ± 0.097
0.0IleXaa: 0.0 ± 0.0
Lys
5.726LysAla: 5.726 ± 0.186
0.681LysCys: 0.681 ± 0.052
4.211LysAsp: 4.211 ± 0.141
4.698LysGlu: 4.698 ± 0.186
1.957LysPhe: 1.957 ± 0.103
4.545LysGly: 4.545 ± 0.156
1.655LysHis: 1.655 ± 0.091
4.194LysIle: 4.194 ± 0.155
5.643LysLys: 5.643 ± 0.22
5.156LysLeu: 5.156 ± 0.176
1.858LysMet: 1.858 ± 0.094
3.022LysAsn: 3.022 ± 0.127
2.815LysPro: 2.815 ± 0.124
2.316LysGln: 2.316 ± 0.134
3.43LysArg: 3.43 ± 0.14
4.14LysSer: 4.14 ± 0.156
3.814LysThr: 3.814 ± 0.115
4.281LysVal: 4.281 ± 0.137
0.954LysTrp: 0.954 ± 0.073
2.134LysTyr: 2.134 ± 0.096
0.0LysXaa: 0.0 ± 0.0
Leu
7.781LeuAla: 7.781 ± 0.194
1.362LeuCys: 1.362 ± 0.074
5.4LeuAsp: 5.4 ± 0.158
5.903LeuGlu: 5.903 ± 0.194
3.393LeuPhe: 3.393 ± 0.147
6.415LeuGly: 6.415 ± 0.167
1.664LeuHis: 1.664 ± 0.092
5.404LeuIle: 5.404 ± 0.188
6.143LeuLys: 6.143 ± 0.207
8.227LeuLeu: 8.227 ± 0.259
2.155LeuMet: 2.155 ± 0.104
3.447LeuAsn: 3.447 ± 0.111
3.938LeuPro: 3.938 ± 0.133
2.58LeuGln: 2.58 ± 0.13
4.268LeuArg: 4.268 ± 0.144
6.262LeuSer: 6.262 ± 0.175
4.657LeuThr: 4.657 ± 0.137
5.656LeuVal: 5.656 ± 0.171
1.011LeuTrp: 1.011 ± 0.074
2.601LeuTyr: 2.601 ± 0.109
0.0LeuXaa: 0.0 ± 0.0
Met
2.411MetAla: 2.411 ± 0.097
0.359MetCys: 0.359 ± 0.043
1.441MetAsp: 1.441 ± 0.072
1.606MetGlu: 1.606 ± 0.09
1.036MetPhe: 1.036 ± 0.076
2.031MetGly: 2.031 ± 0.099
0.479MetHis: 0.479 ± 0.049
1.581MetIle: 1.581 ± 0.088
2.275MetLys: 2.275 ± 0.093
2.584MetLeu: 2.584 ± 0.102
0.85MetMet: 0.85 ± 0.064
1.375MetAsn: 1.375 ± 0.084
1.391MetPro: 1.391 ± 0.086
0.846MetGln: 0.846 ± 0.053
1.342MetArg: 1.342 ± 0.084
1.837MetSer: 1.837 ± 0.096
1.602MetThr: 1.602 ± 0.094
1.701MetVal: 1.701 ± 0.082
0.334MetTrp: 0.334 ± 0.034
0.656MetTyr: 0.656 ± 0.055
0.0MetXaa: 0.0 ± 0.0
Asn
3.034AsnAla: 3.034 ± 0.113
0.561AsnCys: 0.561 ± 0.059
2.51AsnAsp: 2.51 ± 0.119
2.485AsnGlu: 2.485 ± 0.102
1.342AsnPhe: 1.342 ± 0.078
3.026AsnGly: 3.026 ± 0.152
0.801AsnHis: 0.801 ± 0.06
3.232AsnIle: 3.232 ± 0.107
2.576AsnLys: 2.576 ± 0.118
3.348AsnLeu: 3.348 ± 0.117
1.284AsnMet: 1.284 ± 0.075
1.899AsnAsn: 1.899 ± 0.109
2.031AsnPro: 2.031 ± 0.095
1.053AsnGln: 1.053 ± 0.075
1.862AsnArg: 1.862 ± 0.082
2.46AsnSer: 2.46 ± 0.101
2.171AsnThr: 2.171 ± 0.114
2.844AsnVal: 2.844 ± 0.128
0.698AsnTrp: 0.698 ± 0.064
1.499AsnTyr: 1.499 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
3.319ProAla: 3.319 ± 0.131
0.38ProCys: 0.38 ± 0.044
2.985ProAsp: 2.985 ± 0.1
3.901ProGlu: 3.901 ± 0.148
1.829ProPhe: 1.829 ± 0.084
3.29ProGly: 3.29 ± 0.11
0.921ProHis: 0.921 ± 0.06
2.101ProIle: 2.101 ± 0.092
2.266ProLys: 2.266 ± 0.118
3.133ProLeu: 3.133 ± 0.111
1.036ProMet: 1.036 ± 0.068
1.515ProAsn: 1.515 ± 0.089
1.569ProPro: 1.569 ± 0.098
1.098ProGln: 1.098 ± 0.071
1.812ProArg: 1.812 ± 0.09
2.683ProSer: 2.683 ± 0.118
1.92ProThr: 1.92 ± 0.086
3.43ProVal: 3.43 ± 0.121
0.537ProTrp: 0.537 ± 0.059
1.263ProTyr: 1.263 ± 0.066
0.0ProXaa: 0.0 ± 0.0
Gln
2.27GlnAla: 2.27 ± 0.101
0.318GlnCys: 0.318 ± 0.038
1.668GlnAsp: 1.668 ± 0.088
2.089GlnGlu: 2.089 ± 0.133
1.036GlnPhe: 1.036 ± 0.075
1.903GlnGly: 1.903 ± 0.083
0.553GlnHis: 0.553 ± 0.052
1.841GlnIle: 1.841 ± 0.098
2.25GlnLys: 2.25 ± 0.136
2.625GlnLeu: 2.625 ± 0.123
0.912GlnMet: 0.912 ± 0.056
1.238GlnAsn: 1.238 ± 0.079
1.102GlnPro: 1.102 ± 0.067
1.02GlnGln: 1.02 ± 0.075
1.428GlnArg: 1.428 ± 0.092
1.664GlnSer: 1.664 ± 0.079
1.387GlnThr: 1.387 ± 0.082
1.957GlnVal: 1.957 ± 0.09
0.454GlnTrp: 0.454 ± 0.054
1.11GlnTyr: 1.11 ± 0.078
0.0GlnXaa: 0.0 ± 0.0
Arg
3.352ArgAla: 3.352 ± 0.131
0.652ArgCys: 0.652 ± 0.052
2.72ArgAsp: 2.72 ± 0.118
3.414ArgGlu: 3.414 ± 0.132
2.308ArgPhe: 2.308 ± 0.103
3.179ArgGly: 3.179 ± 0.118
1.003ArgHis: 1.003 ± 0.065
3.344ArgIle: 3.344 ± 0.119
3.686ArgLys: 3.686 ± 0.146
4.231ArgLeu: 4.231 ± 0.14
1.412ArgMet: 1.412 ± 0.086
2.056ArgAsn: 2.056 ± 0.09
1.738ArgPro: 1.738 ± 0.1
1.56ArgGln: 1.56 ± 0.099
2.712ArgArg: 2.712 ± 0.129
2.985ArgSer: 2.985 ± 0.108
2.328ArgThr: 2.328 ± 0.091
3.042ArgVal: 3.042 ± 0.12
0.735ArgTrp: 0.735 ± 0.053
1.713ArgTyr: 1.713 ± 0.085
0.0ArgXaa: 0.0 ± 0.0
Ser
5.441SerAla: 5.441 ± 0.168
0.925SerCys: 0.925 ± 0.074
4.326SerAsp: 4.326 ± 0.159
4.128SerGlu: 4.128 ± 0.145
2.873SerPhe: 2.873 ± 0.106
5.779SerGly: 5.779 ± 0.186
1.251SerHis: 1.251 ± 0.075
4.368SerIle: 4.368 ± 0.142
3.806SerLys: 3.806 ± 0.152
6.089SerLeu: 6.089 ± 0.154
1.767SerMet: 1.767 ± 0.092
2.39SerAsn: 2.39 ± 0.109
2.605SerPro: 2.605 ± 0.124
1.705SerGln: 1.705 ± 0.09
3.538SerArg: 3.538 ± 0.119
4.809SerSer: 4.809 ± 0.192
3.17SerThr: 3.17 ± 0.124
4.764SerVal: 4.764 ± 0.166
1.032SerTrp: 1.032 ± 0.068
2.204SerTyr: 2.204 ± 0.103
0.0SerXaa: 0.0 ± 0.0
Thr
4.355ThrAla: 4.355 ± 0.137
0.52ThrCys: 0.52 ± 0.047
3.373ThrAsp: 3.373 ± 0.144
3.315ThrGlu: 3.315 ± 0.165
2.105ThrPhe: 2.105 ± 0.094
4.207ThrGly: 4.207 ± 0.18
0.987ThrHis: 0.987 ± 0.057
3.319ThrIle: 3.319 ± 0.134
2.898ThrLys: 2.898 ± 0.119
4.578ThrLeu: 4.578 ± 0.138
1.226ThrMet: 1.226 ± 0.07
2.142ThrAsn: 2.142 ± 0.19
2.675ThrPro: 2.675 ± 0.115
1.317ThrGln: 1.317 ± 0.078
2.27ThrArg: 2.27 ± 0.101
3.385ThrSer: 3.385 ± 0.152
3.042ThrThr: 3.042 ± 0.153
3.992ThrVal: 3.992 ± 0.132
0.797ThrTrp: 0.797 ± 0.077
1.573ThrTyr: 1.573 ± 0.098
0.0ThrXaa: 0.0 ± 0.0
Val
5.424ValAla: 5.424 ± 0.176
0.978ValCys: 0.978 ± 0.067
4.074ValAsp: 4.074 ± 0.142
4.487ValGlu: 4.487 ± 0.124
3.236ValPhe: 3.236 ± 0.116
4.623ValGly: 4.623 ± 0.165
1.313ValHis: 1.313 ± 0.068
4.52ValIle: 4.52 ± 0.129
4.335ValLys: 4.335 ± 0.149
6.601ValLeu: 6.601 ± 0.2
2.006ValMet: 2.006 ± 0.101
2.658ValAsn: 2.658 ± 0.103
3.038ValPro: 3.038 ± 0.114
1.961ValGln: 1.961 ± 0.093
3.154ValArg: 3.154 ± 0.127
5.329ValSer: 5.329 ± 0.175
3.773ValThr: 3.773 ± 0.14
5.338ValVal: 5.338 ± 0.177
0.863ValTrp: 0.863 ± 0.062
2.275ValTyr: 2.275 ± 0.118
0.0ValXaa: 0.0 ± 0.0
Trp
1.127TrpAla: 1.127 ± 0.081
0.206TrpCys: 0.206 ± 0.026
0.904TrpAsp: 0.904 ± 0.079
0.809TrpGlu: 0.809 ± 0.061
0.611TrpPhe: 0.611 ± 0.049
1.143TrpGly: 1.143 ± 0.099
0.396TrpHis: 0.396 ± 0.041
0.879TrpIle: 0.879 ± 0.053
0.958TrpLys: 0.958 ± 0.066
1.214TrpLeu: 1.214 ± 0.075
0.4TrpMet: 0.4 ± 0.04
0.615TrpAsn: 0.615 ± 0.057
0.66TrpPro: 0.66 ± 0.075
0.533TrpGln: 0.533 ± 0.057
0.805TrpArg: 0.805 ± 0.073
0.929TrpSer: 0.929 ± 0.068
0.743TrpThr: 0.743 ± 0.068
0.859TrpVal: 0.859 ± 0.064
0.173TrpTrp: 0.173 ± 0.027
0.549TrpTyr: 0.549 ± 0.069
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.27TyrAla: 2.27 ± 0.102
0.541TyrCys: 0.541 ± 0.05
2.299TyrAsp: 2.299 ± 0.111
1.99TyrGlu: 1.99 ± 0.094
1.432TyrPhe: 1.432 ± 0.08
2.312TyrGly: 2.312 ± 0.11
0.764TyrHis: 0.764 ± 0.06
1.969TyrIle: 1.969 ± 0.097
1.763TyrLys: 1.763 ± 0.088
2.877TyrLeu: 2.877 ± 0.124
0.788TyrMet: 0.788 ± 0.069
1.412TyrAsn: 1.412 ± 0.085
1.338TyrPro: 1.338 ± 0.074
0.974TyrGln: 0.974 ± 0.072
1.812TyrArg: 1.812 ± 0.1
2.448TyrSer: 2.448 ± 0.123
1.713TyrThr: 1.713 ± 0.095
2.097TyrVal: 2.097 ± 0.1
0.566TyrTrp: 0.566 ± 0.053
1.457TyrTyr: 1.457 ± 0.103
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 777 proteins (242243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski