Cervus elaphus hippelaphus (European red deer)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla; Ruminantia;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A212CJ78|A0A212CJ78_CEREH Glucose transporter type 2 liver (Fragment) OS=Cervus elaphus hippelaphus OX=46360 GN=Celaphus_00012495 PE=3 SV=1
DDD2 pKa = 4.76EE3 pKa = 4.82YY4 pKa = 11.1DD5 pKa = 3.42CAICLDDD12 pKa = 3.98EE13 pKa = 5.89YY14 pKa = 11.41EE15 pKa = 5.75DD16 pKa = 4.2DDD18 pKa = 3.87KK19 pKa = 11.16RR20 pKa = 11.84ILPCSHHH27 pKa = 6.97DDD29 pKa = 3.48DDD31 pKa = 5.33DDD33 pKa = 3.48DDD35 pKa = 4.09SQEEE39 pKa = 3.75EE40 pKa = 4.57EEE42 pKa = 4.36SEEE45 pKa = 4.46HH46 pKa = 5.78PLLRR50 pKa = 11.84PVASASTQSFGALSEEE66 pKa = 4.46RR67 pKa = 11.84SHHH70 pKa = 6.89NMTEEE75 pKa = 3.94SDDD78 pKa = 3.76YY79 pKa = 11.56EE80 pKa = 4.05EE81 pKa = 6.09DD82 pKa = 4.1DD83 pKa = 4.35EEE85 pKa = 4.56DD86 pKa = 4.06EEE88 pKa = 5.52SDDD91 pKa = 4.25EEE93 pKa = 4.26EEE95 pKa = 4.3NEEE98 pKa = 4.27HH99 pKa = 5.67VVVQLQPNGDDD110 pKa = 3.52DDD112 pKa = 3.97YY113 pKa = 11.91LANTV

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A212CAB5|A0A212CAB5_CEREH E2F1 OS=Cervus elaphus hippelaphus OX=46360 GN=Celaphus_00007899 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 10.26GRR4 pKa = 11.84QRR6 pKa = 11.84RR7 pKa = 11.84AGSRR11 pKa = 11.84GPPSSCRR18 pKa = 11.84SSAWSWSPPSMTGAKK33 pKa = 9.52RR34 pKa = 11.84NSSGACRR41 pKa = 11.84AALSRR46 pKa = 11.84SRR48 pKa = 11.84SSLWSGPARR57 pKa = 11.84WSRR60 pKa = 11.84SLWLRR65 pKa = 11.84SWLGTSPRR73 pKa = 11.84SNMATSSLPTASSSRR88 pKa = 11.84ATTSRR93 pKa = 11.84LMRR96 pKa = 11.84APP98 pKa = 3.55

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19236

0

19236

6944429

9

9305

361.0

40.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.267 ± 0.022

2.194 ± 0.015

4.747 ± 0.015

7.043 ± 0.026

3.568 ± 0.012

6.756 ± 0.023

2.542 ± 0.01

4.052 ± 0.018

5.453 ± 0.025

10.159 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.008

3.365 ± 0.015

6.601 ± 0.031

4.777 ± 0.017

5.915 ± 0.019

8.308 ± 0.026

5.209 ± 0.016

6.143 ± 0.016

1.283 ± 0.008

2.51 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski