Streptococcus satellite phage Javan70

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUD9|A0A4D5ZUD9_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan70 OX=2558811 GN=JavanS70_0018 PE=4 SV=1
MM1 pKa = 7.74IDD3 pKa = 5.03DD4 pKa = 6.14IIPEE8 pKa = 4.39LNDD11 pKa = 3.62LQTLATVALVDD22 pKa = 4.68DD23 pKa = 4.85NLNTSQAVLRR33 pKa = 11.84VIEE36 pKa = 4.06QKK38 pKa = 10.11IPRR41 pKa = 11.84LIEE44 pKa = 3.73ILEE47 pKa = 4.05EE48 pKa = 4.31LEE50 pKa = 4.15NGG52 pKa = 3.7

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZYM4|A0A4D5ZYM4_9VIRU HTH DNA-binding protein OS=Streptococcus satellite phage Javan70 OX=2558811 GN=JavanS70_0017 PE=4 SV=1
MM1 pKa = 7.27GRR3 pKa = 11.84YY4 pKa = 7.2NTHH7 pKa = 6.97PAQKK11 pKa = 9.85SGHH14 pKa = 4.57YY15 pKa = 10.21HH16 pKa = 5.5NEE18 pKa = 3.06KK19 pKa = 9.84HH20 pKa = 6.12YY21 pKa = 10.81KK22 pKa = 9.61DD23 pKa = 3.59RR24 pKa = 11.84RR25 pKa = 11.84PTLEE29 pKa = 3.63QQEE32 pKa = 4.28EE33 pKa = 4.17QKK35 pKa = 10.79RR36 pKa = 11.84LKK38 pKa = 10.26KK39 pKa = 10.33LKK41 pKa = 9.56KK42 pKa = 9.5KK43 pKa = 9.73RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.75GRR48 pKa = 3.39

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

3194

48

545

168.1

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.199 ± 0.438

0.532 ± 0.141

5.51 ± 0.53

9.737 ± 0.85

3.663 ± 0.286

4.195 ± 0.508

1.597 ± 0.263

7.483 ± 0.595

9.424 ± 0.502

10.238 ± 0.566

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.258

4.79 ± 0.375

3.225 ± 0.431

4.728 ± 0.362

5.291 ± 0.487

4.508 ± 0.29

6.356 ± 0.458

4.696 ± 0.476

0.971 ± 0.115

4.477 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski