Gemmobacter aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Gemmobacter

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S3MJC1|A0A4S3MJC1_9RHOB NAD-dependent protein deacylase OS=Gemmobacter aestuarii OX=1445661 GN=cobB PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.35VHH4 pKa = 7.26VDD6 pKa = 3.0MSICLLHH13 pKa = 5.73GQCVIAAPQVFSFDD27 pKa = 4.17DD28 pKa = 3.61EE29 pKa = 5.34GGLVWLAEE37 pKa = 4.19VDD39 pKa = 3.5DD40 pKa = 4.28SLRR43 pKa = 11.84ADD45 pKa = 3.48VEE47 pKa = 4.37AASDD51 pKa = 3.75ACPEE55 pKa = 3.94QAIQLGDD62 pKa = 3.25

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S3MT11|A0A4S3MT11_9RHOB Ribonuclease 3 OS=Gemmobacter aestuarii OX=1445661 GN=rnc PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3480

0

3480

1136179

33

3465

326.5

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.48 ± 0.066

0.869 ± 0.013

5.957 ± 0.045

5.539 ± 0.035

3.652 ± 0.026

9.332 ± 0.056

2.045 ± 0.022

4.929 ± 0.025

2.616 ± 0.032

10.114 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.02

2.325 ± 0.023

5.451 ± 0.036

2.804 ± 0.019

7.446 ± 0.04

4.512 ± 0.025

5.33 ± 0.032

7.387 ± 0.033

1.447 ± 0.02

2.023 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski