Microbacterium ginsengisoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0M2V3|A0A0F0M2V3_9MICO Uncharacterized protein OS=Microbacterium ginsengisoli OX=400772 GN=RR49_00418 PE=4 SV=1
MM1 pKa = 7.4SFVNNSTSEE10 pKa = 4.0EE11 pKa = 4.16FEE13 pKa = 5.81DD14 pKa = 3.48EE15 pKa = 4.44DD16 pKa = 4.46EE17 pKa = 4.59YY18 pKa = 11.4LRR20 pKa = 11.84SMKK23 pKa = 10.19QDD25 pKa = 3.31DD26 pKa = 4.88SYY28 pKa = 11.8QFSYY32 pKa = 10.68DD33 pKa = 3.45YY34 pKa = 10.83EE35 pKa = 4.34YY36 pKa = 11.41VADD39 pKa = 4.6RR40 pKa = 11.84FGDD43 pKa = 3.66GDD45 pKa = 4.96DD46 pKa = 4.52DD47 pKa = 4.67VKK49 pKa = 11.51LEE51 pKa = 3.89NARR54 pKa = 11.84LNVSLTWDD62 pKa = 4.09DD63 pKa = 4.1YY64 pKa = 11.09SAPGYY69 pKa = 9.1VVSYY73 pKa = 9.5TVDD76 pKa = 3.3SPTPIPNDD84 pKa = 3.14WTGDD88 pKa = 3.15ADD90 pKa = 5.21QIFNDD95 pKa = 3.31LWLAVTADD103 pKa = 4.31LSSLGIGSQLHH114 pKa = 6.41KK115 pKa = 10.54DD116 pKa = 3.28WPII119 pKa = 3.81

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0LRZ5|A0A0F0LRZ5_9MICO 30S ribosomal protein S6 OS=Microbacterium ginsengisoli OX=400772 GN=rpsF PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.63GRR40 pKa = 11.84ATLSAA45 pKa = 4.08

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2779

0

2779

879300

29

2612

316.4

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.31 ± 0.071

0.497 ± 0.011

6.179 ± 0.038

5.097 ± 0.048

3.045 ± 0.028

8.913 ± 0.043

1.989 ± 0.023

4.54 ± 0.036

1.828 ± 0.038

10.073 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.624 ± 0.017

1.833 ± 0.026

5.372 ± 0.032

2.758 ± 0.026

7.379 ± 0.057

5.542 ± 0.041

6.295 ± 0.044

9.298 ± 0.047

1.485 ± 0.02

1.943 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski