Halogranum gelatinilyticum
Average proteome isoelectric point is 4.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3752 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G9TGR2|A0A1G9TGR2_9EURY Uncharacterized protein OS=Halogranum gelatinilyticum OX=660521 GN=SAMN04487949_1768 PE=4 SV=1
MM1 pKa = 6.93 TQDD4 pKa = 3.46 GTPDD8 pKa = 3.17 LNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 FLQATGAVGLAGALGGVASATPGRR37 pKa = 11.84 SPGPKK42 pKa = 8.82 EE43 pKa = 4.72 DD44 pKa = 4.33 EE45 pKa = 4.17 ILVGVAQGDD54 pKa = 3.95 DD55 pKa = 3.63 SPADD59 pKa = 3.66 VVKK62 pKa = 10.74 KK63 pKa = 10.36 AVPGKK68 pKa = 10.58 ARR70 pKa = 11.84 IVHH73 pKa = 5.44 EE74 pKa = 4.7 NKK76 pKa = 8.83 TLSYY80 pKa = 10.49 VAVKK84 pKa = 10.25 FPSQAADD91 pKa = 3.24 VARR94 pKa = 11.84 EE95 pKa = 3.92 NFIEE99 pKa = 4.7 AVTKK103 pKa = 10.47 NDD105 pKa = 3.13 RR106 pKa = 11.84 VKK108 pKa = 10.83 YY109 pKa = 9.58 AEE111 pKa = 4.2 KK112 pKa = 10.57 NATLSTSLSPNDD124 pKa = 3.57 PRR126 pKa = 11.84 YY127 pKa = 10.03 GDD129 pKa = 3.3 QYY131 pKa = 11.1 APQMVNADD139 pKa = 4.21 TAWDD143 pKa = 3.85 TTLGSSSVTIAVVDD157 pKa = 3.4 TGVQYY162 pKa = 10.47 DD163 pKa = 4.0 HH164 pKa = 7.89 PDD166 pKa = 3.15 LAGSFGSNPGYY177 pKa = 10.95 DD178 pKa = 3.63 FVDD181 pKa = 4.6 DD182 pKa = 5.92 DD183 pKa = 4.79 GDD185 pKa = 4.03 PYY187 pKa = 11.25 PDD189 pKa = 3.39 VLADD193 pKa = 4.19 EE194 pKa = 4.48 YY195 pKa = 11.08 HH196 pKa = 5.62 GTHH199 pKa = 5.66 VAGIAGGVTDD209 pKa = 4.69 NGTGTSGISNSTLISGRR226 pKa = 11.84 ALDD229 pKa = 3.64 EE230 pKa = 4.35 SGRR233 pKa = 11.84 GSTADD238 pKa = 2.99 IADD241 pKa = 3.79 AVEE244 pKa = 3.79 WAADD248 pKa = 3.45 QGADD252 pKa = 4.73 VINLSLGGGGYY263 pKa = 9.07 TSTMKK268 pKa = 10.69 NAVSYY273 pKa = 8.31 ATNNGALVVAAAGNNGTSSVSYY295 pKa = 8.65 PAAYY299 pKa = 9.87 SEE301 pKa = 4.4 CLAVSSLDD309 pKa = 3.55 PDD311 pKa = 4.01 GSLASYY317 pKa = 9.41 SQYY320 pKa = 11.02 GSSIEE325 pKa = 3.94 LAAPGSNVLATLPTDD340 pKa = 4.4 DD341 pKa = 4.44 YY342 pKa = 11.7 GEE344 pKa = 4.35 LSGTSMATPVVAGVAGLTLAQWDD367 pKa = 3.91 VDD369 pKa = 3.72 NVTLRR374 pKa = 11.84 DD375 pKa = 3.81 HH376 pKa = 7.11 LKK378 pKa = 8.74 NTAVDD383 pKa = 3.33 IGLSSSQQGSGRR395 pKa = 11.84 VDD397 pKa = 2.82 AANAVEE403 pKa = 4.18 TDD405 pKa = 3.59 PANGGGGDD413 pKa = 4.04 GGDD416 pKa = 3.92 GGDD419 pKa = 4.46 DD420 pKa = 3.76 GGSGGSTSASVTDD433 pKa = 3.99 SLTNSADD440 pKa = 4.01 SKK442 pKa = 9.22 CWTYY446 pKa = 11.48 AFEE449 pKa = 4.3 YY450 pKa = 10.05 SNPSTVVISLDD461 pKa = 3.48 GPSYY465 pKa = 11.73 ADD467 pKa = 3.37 FDD469 pKa = 5.07 LYY471 pKa = 11.57 ANDD474 pKa = 4.17 GTGTCPSTSSYY485 pKa = 11.27 DD486 pKa = 3.55 YY487 pKa = 10.99 RR488 pKa = 11.84 SWTTTSQEE496 pKa = 4.7 SITIDD501 pKa = 4.02 NPDD504 pKa = 3.15 TSADD508 pKa = 3.55 LSILVDD514 pKa = 3.83 SYY516 pKa = 11.69 SGSGSYY522 pKa = 8.54 TLEE525 pKa = 3.68 IVEE528 pKa = 4.42 YY529 pKa = 9.92 EE530 pKa = 4.0
Molecular weight: 54.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.617
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.681
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.821
Sillero 3.986
Patrickios 1.443
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A1G9ZGJ2|A0A1G9ZGJ2_9EURY Thiamine transport system substrate-binding protein OS=Halogranum gelatinilyticum OX=660521 GN=SAMN04487949_3629 PE=4 SV=1
MM1 pKa = 7.52 SPLGAAPLLAPEE13 pKa = 5.43 KK14 pKa = 10.34 IPSRR18 pKa = 11.84 SLRR21 pKa = 11.84 SLPTASPPSILVGFWVEE38 pKa = 3.92 HH39 pKa = 5.91 PRR41 pKa = 11.84 FSVEE45 pKa = 3.19 KK46 pKa = 10.49 RR47 pKa = 11.84 MTLRR51 pKa = 11.84 CSTHH55 pKa = 5.38 FLEE58 pKa = 5.13 MVNVPPKK65 pKa = 10.32 FRR67 pKa = 11.84 IGGTPRR73 pKa = 11.84 SLAILWLEE81 pKa = 4.13 ANRR84 pKa = 11.84 KK85 pKa = 9.02 NGWNTPCFTVCRR97 pKa = 11.84 YY98 pKa = 9.22 PISLPSLPPLIQYY111 pKa = 9.68 HH112 pKa = 6.52 DD113 pKa = 3.93 PEE115 pKa = 4.53 KK116 pKa = 10.92 NRR118 pKa = 11.84 YY119 pKa = 5.4 RR120 pKa = 11.84 TQRR123 pKa = 11.84 KK124 pKa = 8.83 RR125 pKa = 11.84 EE126 pKa = 4.05 QVPHH130 pKa = 5.64 QAVADD135 pKa = 4.71 RR136 pKa = 11.84 SQIQCDD142 pKa = 3.55 FRR144 pKa = 11.84 AFVRR148 pKa = 11.84 TNSGSQGRR156 pKa = 11.84 GHH158 pKa = 6.18 VALPTLHH165 pKa = 6.74 PRR167 pKa = 11.84 ITAPLNRR174 pKa = 11.84 VSSQLLPCANFLSRR188 pKa = 11.84 YY189 pKa = 8.79 VIYY192 pKa = 10.64 KK193 pKa = 10.22 RR194 pKa = 11.84 ALLRR198 pKa = 11.84 NTGDD202 pKa = 3.3 SDD204 pKa = 3.8 RR205 pKa = 11.84 QTSEE209 pKa = 4.02 LALEE213 pKa = 4.34 TTLLITPIITSRR225 pKa = 11.84 LL226 pKa = 3.49
Molecular weight: 25.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.721
IPC_protein 10.774
Toseland 10.804
ProMoST 10.716
Dawson 10.891
Bjellqvist 10.687
Wikipedia 11.169
Rodwell 10.935
Grimsley 10.95
Solomon 11.096
Lehninger 11.052
Nozaki 10.818
DTASelect 10.687
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 10.643
IPC_peptide 11.096
IPC2_peptide 10.014
IPC2.peptide.svr19 8.572
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3752
0
3752
1098223
39
1990
292.7
31.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.67 ± 0.058
0.682 ± 0.013
8.315 ± 0.048
8.046 ± 0.052
3.449 ± 0.026
8.567 ± 0.039
1.983 ± 0.021
3.644 ± 0.034
2.057 ± 0.03
9.226 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.753 ± 0.019
2.425 ± 0.025
4.561 ± 0.026
2.641 ± 0.024
6.334 ± 0.033
5.624 ± 0.032
6.605 ± 0.028
9.541 ± 0.042
1.155 ± 0.016
2.722 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here