Bifidobacterium aquikefiri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261GBM0|A0A261GBM0_9BIFI Solute-binding protein of ABC transporter OS=Bifidobacterium aquikefiri OX=1653207 GN=BAQU_0193 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84TLRR6 pKa = 11.84ISIEE10 pKa = 3.98QPSEE14 pKa = 4.75SNHH17 pKa = 4.82TWNKK21 pKa = 8.89CDD23 pKa = 3.45TYY25 pKa = 11.27VHH27 pKa = 6.33SEE29 pKa = 4.3GIGNDD34 pKa = 3.59DD35 pKa = 3.71ADD37 pKa = 4.14QIISSMDD44 pKa = 3.64TIAPDD49 pKa = 3.24VWHH52 pKa = 7.2LEE54 pKa = 3.95NDD56 pKa = 4.4CGDD59 pKa = 3.16EE60 pKa = 4.19WEE62 pKa = 4.88ISTEE66 pKa = 4.09QYY68 pKa = 10.3EE69 pKa = 4.41QLQSEE74 pKa = 4.77HH75 pKa = 6.59EE76 pKa = 4.28KK77 pKa = 10.73CSPSRR82 pKa = 11.84FTLPDD87 pKa = 3.68LPQTFTTDD95 pKa = 3.26YY96 pKa = 9.41VTVSINN102 pKa = 2.91

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261GBX9|A0A261GBX9_9BIFI L-asparagine permease OS=Bifidobacterium aquikefiri OX=1653207 GN=BAQU_0304 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1995

0

1995

702279

29

5278

352.0

38.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.808 ± 0.053

0.859 ± 0.02

6.053 ± 0.053

5.348 ± 0.051

3.558 ± 0.037

7.507 ± 0.05

2.467 ± 0.03

6.19 ± 0.051

4.08 ± 0.048

8.972 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.03

3.732 ± 0.038

4.279 ± 0.032

3.979 ± 0.038

5.51 ± 0.064

7.578 ± 0.068

6.154 ± 0.059

7.361 ± 0.048

1.315 ± 0.023

2.679 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski