Gordonia phage ThankyouJordi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; Nymphadoravirus; unclassified Nymphadoravirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EGF0|A0A4Y6EGF0_9CAUD Uncharacterized protein OS=Gordonia phage ThankyouJordi OX=2571252 GN=39 PE=4 SV=1
MM1 pKa = 6.88STTRR5 pKa = 11.84RR6 pKa = 11.84LLALGAAALVLVAGCASDD24 pKa = 4.11DD25 pKa = 3.69GGYY28 pKa = 10.32SSVVGGTTPATTTATGTTSTTKK50 pKa = 10.27PLPTLTQLNEE60 pKa = 3.99KK61 pKa = 9.71VDD63 pKa = 4.14LVGSEE68 pKa = 4.07GQDD71 pKa = 2.85MVVYY75 pKa = 9.82SVTSIAVDD83 pKa = 4.27GEE85 pKa = 4.7CPGDD89 pKa = 3.89FYY91 pKa = 11.82NGVEE95 pKa = 4.11NGHH98 pKa = 6.65LLVVGLDD105 pKa = 3.62VEE107 pKa = 4.93TGPDD111 pKa = 2.86WDD113 pKa = 4.24AQFGDD118 pKa = 3.35IFRR121 pKa = 11.84PEE123 pKa = 3.68SKK125 pKa = 9.27YY126 pKa = 10.45WSIIGTDD133 pKa = 4.68GITEE137 pKa = 4.11SGITSPASYY146 pKa = 10.58GCAVDD151 pKa = 4.92QIPKK155 pKa = 10.46SFDD158 pKa = 3.26PDD160 pKa = 2.91SRR162 pKa = 11.84YY163 pKa = 10.04QFSIAFDD170 pKa = 3.5TRR172 pKa = 11.84NASGMLQHH180 pKa = 6.96RR181 pKa = 11.84VGEE184 pKa = 4.67SVSRR188 pKa = 11.84WQYY191 pKa = 10.59

Molecular weight: 20.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EKB0|A0A4Y6EKB0_9CAUD Lysin A L-Ala-D-Glu peptidase domain OS=Gordonia phage ThankyouJordi OX=2571252 GN=19 PE=4 SV=1
MM1 pKa = 7.34SGPRR5 pKa = 11.84VRR7 pKa = 11.84APLTRR12 pKa = 11.84SRR14 pKa = 11.84LSMADD19 pKa = 3.17LLARR23 pKa = 11.84FFTQPFTVRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84TGDD37 pKa = 3.45GAVGPIYY44 pKa = 10.43ADD46 pKa = 3.25PVTLQGRR53 pKa = 11.84VNATNRR59 pKa = 11.84LVIDD63 pKa = 3.7DD64 pKa = 4.91RR65 pKa = 11.84GNQVLSAAKK74 pKa = 9.52ISMSVTEE81 pKa = 4.96DD82 pKa = 4.45DD83 pKa = 4.41IPTGSQVRR91 pKa = 11.84VGDD94 pKa = 4.15GPWRR98 pKa = 11.84TVIAEE103 pKa = 4.04SRR105 pKa = 11.84HH106 pKa = 4.74VGGFRR111 pKa = 11.84KK112 pKa = 10.35SPDD115 pKa = 3.47YY116 pKa = 11.31YY117 pKa = 11.38SIDD120 pKa = 3.74LNN122 pKa = 3.96

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

17422

38

2147

209.9

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.876 ± 0.561

0.838 ± 0.163

6.871 ± 0.41

5.654 ± 0.272

2.675 ± 0.179

8.484 ± 0.393

2.003 ± 0.217

4.626 ± 0.186

3.404 ± 0.202

7.594 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.138

3.111 ± 0.205

5.55 ± 0.264

3.668 ± 0.29

7.542 ± 0.413

5.66 ± 0.17

6.98 ± 0.246

7.272 ± 0.222

1.906 ± 0.152

2.135 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski