Amino acid dipepetide frequency for Gordonia phage ThankyouJordi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.369AlaAla: 18.369 ± 1.756
1.033AlaCys: 1.033 ± 0.266
7.921AlaAsp: 7.921 ± 0.706
7.405AlaGlu: 7.405 ± 0.789
2.468AlaPhe: 2.468 ± 0.388
9.07AlaGly: 9.07 ± 0.777
2.411AlaHis: 2.411 ± 0.487
5.338AlaIle: 5.338 ± 0.525
4.248AlaLys: 4.248 ± 0.528
8.036AlaLeu: 8.036 ± 0.719
3.387AlaMet: 3.387 ± 0.498
3.502AlaAsn: 3.502 ± 0.456
5.224AlaPro: 5.224 ± 0.546
4.879AlaGln: 4.879 ± 0.548
6.773AlaArg: 6.773 ± 0.608
6.601AlaSer: 6.601 ± 1.02
8.438AlaThr: 8.438 ± 1.035
9.07AlaVal: 9.07 ± 0.81
2.009AlaTrp: 2.009 ± 0.339
2.583AlaTyr: 2.583 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.861CysAla: 0.861 ± 0.289
0.115CysCys: 0.115 ± 0.065
0.746CysAsp: 0.746 ± 0.285
0.804CysGlu: 0.804 ± 0.224
0.115CysPhe: 0.115 ± 0.089
1.263CysGly: 1.263 ± 0.429
0.172CysHis: 0.172 ± 0.112
0.057CysIle: 0.057 ± 0.074
0.172CysLys: 0.172 ± 0.092
0.344CysLeu: 0.344 ± 0.156
0.23CysMet: 0.23 ± 0.108
0.402CysAsn: 0.402 ± 0.147
0.402CysPro: 0.402 ± 0.159
0.23CysGln: 0.23 ± 0.127
0.631CysArg: 0.631 ± 0.224
0.459CysSer: 0.459 ± 0.223
0.517CysThr: 0.517 ± 0.171
0.287CysVal: 0.287 ± 0.137
0.287CysTrp: 0.287 ± 0.109
0.287CysTyr: 0.287 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
6.429AspAla: 6.429 ± 0.785
0.574AspCys: 0.574 ± 0.232
6.314AspAsp: 6.314 ± 0.999
4.19AspGlu: 4.19 ± 0.662
1.55AspPhe: 1.55 ± 0.313
6.027AspGly: 6.027 ± 0.762
1.837AspHis: 1.837 ± 0.322
2.411AspIle: 2.411 ± 0.396
1.837AspLys: 1.837 ± 0.282
7.003AspLeu: 7.003 ± 0.692
0.976AspMet: 0.976 ± 0.286
2.411AspAsn: 2.411 ± 0.463
5.281AspPro: 5.281 ± 0.512
1.952AspGln: 1.952 ± 0.31
5.051AspArg: 5.051 ± 0.621
3.157AspSer: 3.157 ± 0.498
3.272AspThr: 3.272 ± 0.47
5.166AspVal: 5.166 ± 0.558
1.894AspTrp: 1.894 ± 0.358
1.378AspTyr: 1.378 ± 0.252
0.0AspXaa: 0.0 ± 0.0
Glu
6.486GluAla: 6.486 ± 0.751
0.746GluCys: 0.746 ± 0.239
2.928GluAsp: 2.928 ± 0.385
3.616GluGlu: 3.616 ± 0.506
1.894GluPhe: 1.894 ± 0.341
3.444GluGly: 3.444 ± 0.515
0.976GluHis: 0.976 ± 0.21
3.1GluIle: 3.1 ± 0.481
2.64GluLys: 2.64 ± 0.429
5.224GluLeu: 5.224 ± 0.647
0.918GluMet: 0.918 ± 0.19
1.722GluAsn: 1.722 ± 0.361
2.468GluPro: 2.468 ± 0.431
2.928GluGln: 2.928 ± 0.431
5.051GluArg: 5.051 ± 0.734
3.157GluSer: 3.157 ± 0.486
3.215GluThr: 3.215 ± 0.366
4.133GluVal: 4.133 ± 0.496
0.976GluTrp: 0.976 ± 0.273
0.918GluTyr: 0.918 ± 0.196
0.0GluXaa: 0.0 ± 0.0
Phe
3.272PheAla: 3.272 ± 0.392
0.057PheCys: 0.057 ± 0.051
2.813PheAsp: 2.813 ± 0.37
1.378PheGlu: 1.378 ± 0.21
0.861PhePhe: 0.861 ± 0.19
2.755PheGly: 2.755 ± 0.391
0.402PheHis: 0.402 ± 0.142
1.091PheIle: 1.091 ± 0.232
1.033PheLys: 1.033 ± 0.267
1.263PheLeu: 1.263 ± 0.24
0.517PheMet: 0.517 ± 0.166
0.804PheAsn: 0.804 ± 0.213
1.32PhePro: 1.32 ± 0.288
0.517PheGln: 0.517 ± 0.175
2.009PheArg: 2.009 ± 0.412
1.32PheSer: 1.32 ± 0.224
1.779PheThr: 1.779 ± 0.282
1.952PheVal: 1.952 ± 0.318
0.918PheTrp: 0.918 ± 0.203
0.689PheTyr: 0.689 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
8.897GlyAla: 8.897 ± 0.813
1.033GlyCys: 1.033 ± 0.373
4.535GlyAsp: 4.535 ± 0.431
4.305GlyGlu: 4.305 ± 0.468
2.181GlyPhe: 2.181 ± 0.358
7.864GlyGly: 7.864 ± 1.162
1.205GlyHis: 1.205 ± 0.31
4.535GlyIle: 4.535 ± 0.43
3.559GlyLys: 3.559 ± 0.412
5.625GlyLeu: 5.625 ± 0.718
2.181GlyMet: 2.181 ± 0.375
3.502GlyAsn: 3.502 ± 0.452
4.018GlyPro: 4.018 ± 0.461
4.018GlyGln: 4.018 ± 0.52
6.199GlyArg: 6.199 ± 0.554
5.568GlySer: 5.568 ± 0.752
5.568GlyThr: 5.568 ± 1.044
6.486GlyVal: 6.486 ± 0.677
1.607GlyTrp: 1.607 ± 0.347
1.894GlyTyr: 1.894 ± 0.272
0.0GlyXaa: 0.0 ± 0.0
His
1.722HisAla: 1.722 ± 0.333
0.172HisCys: 0.172 ± 0.099
0.861HisAsp: 0.861 ± 0.29
1.205HisGlu: 1.205 ± 0.344
0.517HisPhe: 0.517 ± 0.16
1.492HisGly: 1.492 ± 0.246
0.631HisHis: 0.631 ± 0.216
0.746HisIle: 0.746 ± 0.254
0.517HisLys: 0.517 ± 0.169
2.468HisLeu: 2.468 ± 0.453
0.287HisMet: 0.287 ± 0.129
0.344HisAsn: 0.344 ± 0.149
1.607HisPro: 1.607 ± 0.345
0.804HisGln: 0.804 ± 0.214
1.837HisArg: 1.837 ± 0.349
1.091HisSer: 1.091 ± 0.298
1.32HisThr: 1.32 ± 0.24
1.32HisVal: 1.32 ± 0.344
0.344HisTrp: 0.344 ± 0.143
0.746HisTyr: 0.746 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
5.855IleAla: 5.855 ± 0.734
0.344IleCys: 0.344 ± 0.132
4.879IleAsp: 4.879 ± 0.465
3.789IleGlu: 3.789 ± 0.476
0.689IlePhe: 0.689 ± 0.199
4.19IleGly: 4.19 ± 0.528
0.976IleHis: 0.976 ± 0.229
1.665IleIle: 1.665 ± 0.332
0.976IleLys: 0.976 ± 0.26
2.468IleLeu: 2.468 ± 0.314
0.402IleMet: 0.402 ± 0.12
1.492IleAsn: 1.492 ± 0.296
2.583IlePro: 2.583 ± 0.365
2.181IleGln: 2.181 ± 0.382
3.961IleArg: 3.961 ± 0.461
2.124IleSer: 2.124 ± 0.264
2.928IleThr: 2.928 ± 0.419
3.157IleVal: 3.157 ± 0.486
0.459IleTrp: 0.459 ± 0.169
1.148IleTyr: 1.148 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
4.248LysAla: 4.248 ± 0.703
0.115LysCys: 0.115 ± 0.072
1.665LysAsp: 1.665 ± 0.31
1.607LysGlu: 1.607 ± 0.361
0.918LysPhe: 0.918 ± 0.203
2.468LysGly: 2.468 ± 0.388
0.746LysHis: 0.746 ± 0.188
1.665LysIle: 1.665 ± 0.296
1.263LysLys: 1.263 ± 0.322
2.755LysLeu: 2.755 ± 0.405
0.344LysMet: 0.344 ± 0.138
0.861LysAsn: 0.861 ± 0.253
2.64LysPro: 2.64 ± 0.362
1.263LysGln: 1.263 ± 0.316
2.698LysArg: 2.698 ± 0.478
1.837LysSer: 1.837 ± 0.363
2.583LysThr: 2.583 ± 0.4
2.468LysVal: 2.468 ± 0.442
0.976LysTrp: 0.976 ± 0.223
0.918LysTyr: 0.918 ± 0.193
0.0LysXaa: 0.0 ± 0.0
Leu
10.734LeuAla: 10.734 ± 1.045
0.459LeuCys: 0.459 ± 0.175
5.109LeuAsp: 5.109 ± 0.619
4.305LeuGlu: 4.305 ± 0.551
2.64LeuPhe: 2.64 ± 0.546
5.912LeuGly: 5.912 ± 0.47
1.378LeuHis: 1.378 ± 0.407
3.559LeuIle: 3.559 ± 0.392
2.468LeuLys: 2.468 ± 0.418
5.109LeuLeu: 5.109 ± 0.559
1.722LeuMet: 1.722 ± 0.343
2.296LeuAsn: 2.296 ± 0.385
3.846LeuPro: 3.846 ± 0.483
2.698LeuGln: 2.698 ± 0.32
6.027LeuArg: 6.027 ± 0.656
4.477LeuSer: 4.477 ± 0.527
5.625LeuThr: 5.625 ± 0.571
5.051LeuVal: 5.051 ± 0.645
1.607LeuTrp: 1.607 ± 0.281
0.918LeuTyr: 0.918 ± 0.239
0.0LeuXaa: 0.0 ± 0.0
Met
2.698MetAla: 2.698 ± 0.512
0.23MetCys: 0.23 ± 0.114
0.517MetAsp: 0.517 ± 0.192
0.689MetGlu: 0.689 ± 0.22
0.172MetPhe: 0.172 ± 0.093
1.952MetGly: 1.952 ± 0.408
0.517MetHis: 0.517 ± 0.17
0.689MetIle: 0.689 ± 0.233
0.861MetLys: 0.861 ± 0.26
1.607MetLeu: 1.607 ± 0.322
0.344MetMet: 0.344 ± 0.15
0.804MetAsn: 0.804 ± 0.228
1.32MetPro: 1.32 ± 0.283
0.517MetGln: 0.517 ± 0.173
2.009MetArg: 2.009 ± 0.357
2.009MetSer: 2.009 ± 0.322
2.64MetThr: 2.64 ± 0.344
1.263MetVal: 1.263 ± 0.277
0.459MetTrp: 0.459 ± 0.192
0.23MetTyr: 0.23 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
3.846AsnAla: 3.846 ± 0.469
0.287AsnCys: 0.287 ± 0.131
2.009AsnAsp: 2.009 ± 0.346
1.378AsnGlu: 1.378 ± 0.267
0.689AsnPhe: 0.689 ± 0.239
2.583AsnGly: 2.583 ± 0.407
1.033AsnHis: 1.033 ± 0.32
0.861AsnIle: 0.861 ± 0.215
0.861AsnLys: 0.861 ± 0.219
2.124AsnLeu: 2.124 ± 0.331
0.574AsnMet: 0.574 ± 0.153
1.033AsnAsn: 1.033 ± 0.268
2.124AsnPro: 2.124 ± 0.318
0.804AsnGln: 0.804 ± 0.218
2.928AsnArg: 2.928 ± 0.409
2.296AsnSer: 2.296 ± 0.321
2.411AsnThr: 2.411 ± 0.548
1.894AsnVal: 1.894 ± 0.361
0.517AsnTrp: 0.517 ± 0.142
0.861AsnTyr: 0.861 ± 0.238
0.0AsnXaa: 0.0 ± 0.0
Pro
6.773ProAla: 6.773 ± 0.541
0.402ProCys: 0.402 ± 0.167
4.42ProAsp: 4.42 ± 0.627
3.846ProGlu: 3.846 ± 0.48
1.32ProPhe: 1.32 ± 0.215
6.085ProGly: 6.085 ± 0.594
0.459ProHis: 0.459 ± 0.194
2.64ProIle: 2.64 ± 0.402
1.952ProLys: 1.952 ± 0.361
3.616ProLeu: 3.616 ± 0.485
1.32ProMet: 1.32 ± 0.232
2.124ProAsn: 2.124 ± 0.375
2.813ProPro: 2.813 ± 0.457
1.32ProGln: 1.32 ± 0.286
3.559ProArg: 3.559 ± 0.519
3.157ProSer: 3.157 ± 0.405
4.19ProThr: 4.19 ± 0.556
3.846ProVal: 3.846 ± 0.478
1.033ProTrp: 1.033 ± 0.19
0.631ProTyr: 0.631 ± 0.171
0.0ProXaa: 0.0 ± 0.0
Gln
3.559GlnAla: 3.559 ± 0.474
0.23GlnCys: 0.23 ± 0.134
1.837GlnAsp: 1.837 ± 0.274
1.263GlnGlu: 1.263 ± 0.27
1.091GlnPhe: 1.091 ± 0.253
2.64GlnGly: 2.64 ± 0.555
0.746GlnHis: 0.746 ± 0.195
2.698GlnIle: 2.698 ± 0.391
1.378GlnLys: 1.378 ± 0.303
4.363GlnLeu: 4.363 ± 0.584
0.804GlnMet: 0.804 ± 0.187
1.091GlnAsn: 1.091 ± 0.232
1.894GlnPro: 1.894 ± 0.271
1.492GlnGln: 1.492 ± 0.337
3.329GlnArg: 3.329 ± 0.498
1.779GlnSer: 1.779 ± 0.396
2.066GlnThr: 2.066 ± 0.321
2.583GlnVal: 2.583 ± 0.333
0.918GlnTrp: 0.918 ± 0.208
0.918GlnTyr: 0.918 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
7.233ArgAla: 7.233 ± 0.716
0.804ArgCys: 0.804 ± 0.241
5.109ArgAsp: 5.109 ± 0.703
4.592ArgGlu: 4.592 ± 0.562
2.009ArgPhe: 2.009 ± 0.305
4.592ArgGly: 4.592 ± 0.449
1.779ArgHis: 1.779 ± 0.413
3.789ArgIle: 3.789 ± 0.502
3.272ArgLys: 3.272 ± 0.421
7.233ArgLeu: 7.233 ± 0.658
2.239ArgMet: 2.239 ± 0.389
1.952ArgAsn: 1.952 ± 0.32
3.961ArgPro: 3.961 ± 0.525
2.813ArgGln: 2.813 ± 0.49
6.027ArgArg: 6.027 ± 0.856
3.731ArgSer: 3.731 ± 0.444
5.625ArgThr: 5.625 ± 0.692
4.879ArgVal: 4.879 ± 0.54
1.435ArgTrp: 1.435 ± 0.319
2.353ArgTyr: 2.353 ± 0.314
0.0ArgXaa: 0.0 ± 0.0
Ser
6.773SerAla: 6.773 ± 0.872
0.172SerCys: 0.172 ± 0.096
3.846SerAsp: 3.846 ± 0.423
2.985SerGlu: 2.985 ± 0.409
1.607SerPhe: 1.607 ± 0.268
6.142SerGly: 6.142 ± 0.791
0.631SerHis: 0.631 ± 0.175
3.157SerIle: 3.157 ± 0.512
2.411SerLys: 2.411 ± 0.355
3.616SerLeu: 3.616 ± 0.427
1.607SerMet: 1.607 ± 0.283
1.837SerAsn: 1.837 ± 0.384
2.526SerPro: 2.526 ± 0.401
2.181SerGln: 2.181 ± 0.515
3.846SerArg: 3.846 ± 0.434
2.698SerSer: 2.698 ± 0.329
3.387SerThr: 3.387 ± 0.417
4.42SerVal: 4.42 ± 0.475
1.205SerTrp: 1.205 ± 0.283
1.55SerTyr: 1.55 ± 0.33
0.0SerXaa: 0.0 ± 0.0
Thr
9.07ThrAla: 9.07 ± 1.07
0.517ThrCys: 0.517 ± 0.183
5.453ThrAsp: 5.453 ± 0.626
2.698ThrGlu: 2.698 ± 0.464
1.837ThrPhe: 1.837 ± 0.275
7.347ThrGly: 7.347 ± 0.784
1.205ThrHis: 1.205 ± 0.268
3.616ThrIle: 3.616 ± 0.404
1.55ThrLys: 1.55 ± 0.277
5.051ThrLeu: 5.051 ± 0.488
1.435ThrMet: 1.435 ± 0.268
1.665ThrAsn: 1.665 ± 0.332
4.19ThrPro: 4.19 ± 0.504
1.55ThrGln: 1.55 ± 0.288
5.051ThrArg: 5.051 ± 0.567
4.42ThrSer: 4.42 ± 0.654
4.994ThrThr: 4.994 ± 0.678
5.798ThrVal: 5.798 ± 0.527
0.918ThrTrp: 0.918 ± 0.244
1.435ThrTyr: 1.435 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
8.036ValAla: 8.036 ± 0.663
0.746ValCys: 0.746 ± 0.204
5.166ValAsp: 5.166 ± 0.524
4.707ValGlu: 4.707 ± 0.499
2.755ValPhe: 2.755 ± 0.459
5.338ValGly: 5.338 ± 0.698
1.607ValHis: 1.607 ± 0.304
3.616ValIle: 3.616 ± 0.39
1.894ValLys: 1.894 ± 0.386
4.994ValLeu: 4.994 ± 0.592
1.263ValMet: 1.263 ± 0.275
2.411ValAsn: 2.411 ± 0.333
5.109ValPro: 5.109 ± 0.576
2.526ValGln: 2.526 ± 0.624
4.764ValArg: 4.764 ± 0.518
4.018ValSer: 4.018 ± 0.515
6.142ValThr: 6.142 ± 0.535
5.912ValVal: 5.912 ± 0.828
0.689ValTrp: 0.689 ± 0.172
1.033ValTyr: 1.033 ± 0.203
0.0ValXaa: 0.0 ± 0.0
Trp
1.378TrpAla: 1.378 ± 0.287
0.115TrpCys: 0.115 ± 0.074
1.033TrpAsp: 1.033 ± 0.247
0.976TrpGlu: 0.976 ± 0.265
0.861TrpPhe: 0.861 ± 0.246
1.378TrpGly: 1.378 ± 0.282
0.804TrpHis: 0.804 ± 0.264
0.344TrpIle: 0.344 ± 0.133
0.574TrpLys: 0.574 ± 0.184
1.378TrpLeu: 1.378 ± 0.304
0.402TrpMet: 0.402 ± 0.136
0.517TrpAsn: 0.517 ± 0.149
1.148TrpPro: 1.148 ± 0.202
1.033TrpGln: 1.033 ± 0.272
2.181TrpArg: 2.181 ± 0.411
1.091TrpSer: 1.091 ± 0.27
1.378TrpThr: 1.378 ± 0.238
1.607TrpVal: 1.607 ± 0.339
0.402TrpTrp: 0.402 ± 0.197
0.459TrpTyr: 0.459 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.526TyrAla: 2.526 ± 0.368
0.23TyrCys: 0.23 ± 0.114
1.55TyrAsp: 1.55 ± 0.291
0.804TyrGlu: 0.804 ± 0.255
0.574TyrPhe: 0.574 ± 0.188
2.296TyrGly: 2.296 ± 0.301
0.517TyrHis: 0.517 ± 0.218
0.689TyrIle: 0.689 ± 0.199
0.574TyrLys: 0.574 ± 0.24
1.665TyrLeu: 1.665 ± 0.35
0.517TyrMet: 0.517 ± 0.19
0.344TyrAsn: 0.344 ± 0.135
1.033TyrPro: 1.033 ± 0.246
0.976TyrGln: 0.976 ± 0.243
1.492TyrArg: 1.492 ± 0.34
1.607TyrSer: 1.607 ± 0.236
1.722TyrThr: 1.722 ± 0.371
1.435TyrVal: 1.435 ± 0.296
0.402TyrTrp: 0.402 ± 0.157
0.402TyrTyr: 0.402 ± 0.148
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (17422 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski