Skermania sp. ID1734

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Skermania; unclassified Skermania

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A554V9F2|A0A554V9F2_9ACTN 60 kDa chaperonin OS=Skermania sp. ID1734 OX=2597516 GN=groL PE=3 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84DD3 pKa = 3.51QLPPGLPPDD12 pKa = 4.62PFAGDD17 pKa = 3.63PADD20 pKa = 3.84PAAALDD26 pKa = 4.79AIEE29 pKa = 5.21PGQPLDD35 pKa = 3.61PQEE38 pKa = 4.14RR39 pKa = 11.84LAVEE43 pKa = 4.17EE44 pKa = 4.75DD45 pKa = 3.54LADD48 pKa = 3.98LAVYY52 pKa = 8.37EE53 pKa = 4.65ALLSHH58 pKa = 6.79RR59 pKa = 11.84GIRR62 pKa = 11.84GLVVSCEE69 pKa = 4.31DD70 pKa = 3.83CNSDD74 pKa = 5.42HH75 pKa = 6.33YY76 pKa = 11.23HH77 pKa = 6.58DD78 pKa = 4.26WDD80 pKa = 3.57MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.62EE99 pKa = 4.28PAFDD103 pKa = 4.21PVPEE107 pKa = 5.07AYY109 pKa = 8.64VTWDD113 pKa = 3.22YY114 pKa = 11.65CRR116 pKa = 11.84GYY118 pKa = 11.37ADD120 pKa = 3.97ASMNEE125 pKa = 3.88ALYY128 pKa = 10.96GDD130 pKa = 4.47SGLDD134 pKa = 3.45GG135 pKa = 4.16

Molecular weight: 14.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A554V2Z7|A0A554V2Z7_9ACTN Na+/H+ antiporter subunit D OS=Skermania sp. ID1734 OX=2597516 GN=FOS14_11505 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4709

0

4709

1532270

13

10299

325.4

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.003 ± 0.044

0.839 ± 0.012

6.152 ± 0.028

5.316 ± 0.032

3.019 ± 0.021

8.587 ± 0.029

2.278 ± 0.016

4.425 ± 0.022

2.308 ± 0.027

9.938 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.967 ± 0.013

2.312 ± 0.017

5.625 ± 0.027

3.12 ± 0.02

7.484 ± 0.035

5.494 ± 0.021

5.922 ± 0.027

8.626 ± 0.043

1.473 ± 0.012

2.111 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski