Stenotrophomonas phage S1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B7SYE5|B7SYE5_9CAUD Gp15 OS=Stenotrophomonas phage S1 OX=573591 PE=4 SV=1
MM1 pKa = 7.05IQASAGFLTDD11 pKa = 3.42IGAGLIVVDD20 pKa = 4.75DD21 pKa = 4.62AEE23 pKa = 4.84LGEE26 pKa = 4.72DD27 pKa = 3.45QSGPATLISVQQLSRR42 pKa = 11.84TGGGSAQVTSDD53 pKa = 3.09ASVTIEE59 pKa = 3.82FEE61 pKa = 4.29VPRR64 pKa = 11.84EE65 pKa = 4.03SGEE68 pKa = 3.97VNPRR72 pKa = 11.84LLVHH76 pKa = 6.81RR77 pKa = 11.84ARR79 pKa = 11.84YY80 pKa = 9.59DD81 pKa = 3.99LIRR84 pKa = 11.84VLTFNDD90 pKa = 3.04KK91 pKa = 10.7LLPKK95 pKa = 10.56GITTFEE101 pKa = 4.21LLQSQMATLEE111 pKa = 4.28DD112 pKa = 4.25DD113 pKa = 4.29AGHH116 pKa = 6.05SAVVAQITARR126 pKa = 11.84AGLTEE131 pKa = 4.11TFEE134 pKa = 4.38PVPNPP139 pKa = 3.42

Molecular weight:
14.73 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B7SYG8|B7SYG8_9CAUD Gp38 OS=Stenotrophomonas phage S1 OX=573591 PE=4 SV=1
MM1 pKa = 7.59SARR4 pKa = 11.84PQQIGRR10 pKa = 11.84AAEE13 pKa = 3.95VRR15 pKa = 11.84KK16 pKa = 9.65VLSMFPQGATVEE28 pKa = 4.3QIKK31 pKa = 8.11TAGRR35 pKa = 11.84INSTHH40 pKa = 5.1QAIGYY45 pKa = 4.99TLKK48 pKa = 10.85GLARR52 pKa = 11.84SGQAICHH59 pKa = 6.1RR60 pKa = 11.84SGVRR64 pKa = 11.84GIWRR68 pKa = 11.84LSSHH72 pKa = 5.46TQHH75 pKa = 7.27AIAPLRR81 pKa = 11.84AAPARR86 pKa = 11.84EE87 pKa = 3.88RR88 pKa = 11.84PTCVVGPLTGVSDD101 pKa = 3.78AATTIRR107 pKa = 11.84HH108 pKa = 6.0RR109 pKa = 11.84EE110 pKa = 3.77LDD112 pKa = 3.81RR113 pKa = 11.84QQLADD118 pKa = 4.64DD119 pKa = 5.0LDD121 pKa = 4.41AFLAAGGHH129 pKa = 5.91IEE131 pKa = 4.38VLGHH135 pKa = 5.52TPLRR139 pKa = 11.84PLMSRR144 pKa = 11.84HH145 pKa = 4.86TANHH149 pKa = 5.53GSYY152 pKa = 10.42AEE154 pKa = 3.99RR155 pKa = 11.84MAAHH159 pKa = 6.79EE160 pKa = 4.17MDD162 pKa = 3.44

Molecular weight:
17.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12930

69

1248

269.4

29.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.908 ± 0.769

1.114 ± 0.216

5.731 ± 0.255

5.352 ± 0.346

2.56 ± 0.176

8.268 ± 0.356

1.91 ± 0.207

3.859 ± 0.221

3.155 ± 0.29

8.577 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.138

2.738 ± 0.165

5.391 ± 0.454

4.47 ± 0.362

8.484 ± 0.471

6.551 ± 0.373

5.53 ± 0.319

7.108 ± 0.374

1.895 ± 0.158

2.204 ± 0.185

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski