Stenotrophomonas phage S1
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7SYE5|B7SYE5_9CAUD Gp15 OS=Stenotrophomonas phage S1 OX=573591 PE=4 SV=1
MM1 pKa = 7.05 IQASAGFLTDD11 pKa = 3.42 IGAGLIVVDD20 pKa = 4.75 DD21 pKa = 4.62 AEE23 pKa = 4.84 LGEE26 pKa = 4.72 DD27 pKa = 3.45 QSGPATLISVQQLSRR42 pKa = 11.84 TGGGSAQVTSDD53 pKa = 3.09 ASVTIEE59 pKa = 3.82 FEE61 pKa = 4.29 VPRR64 pKa = 11.84 EE65 pKa = 4.03 SGEE68 pKa = 3.97 VNPRR72 pKa = 11.84 LLVHH76 pKa = 6.81 RR77 pKa = 11.84 ARR79 pKa = 11.84 YY80 pKa = 9.59 DD81 pKa = 3.99 LIRR84 pKa = 11.84 VLTFNDD90 pKa = 3.04 KK91 pKa = 10.7 LLPKK95 pKa = 10.56 GITTFEE101 pKa = 4.21 LLQSQMATLEE111 pKa = 4.28 DD112 pKa = 4.25 DD113 pKa = 4.29 AGHH116 pKa = 6.05 SAVVAQITARR126 pKa = 11.84 AGLTEE131 pKa = 4.11 TFEE134 pKa = 4.38 PVPNPP139 pKa = 3.42
Molecular weight: 14.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.564
IPC2_protein 4.406
IPC_protein 4.304
Toseland 4.139
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.151
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.215
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.151
EMBOSS 4.164
Sillero 4.418
Patrickios 4.113
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.35
Protein with the highest isoelectric point:
>tr|B7SYG8|B7SYG8_9CAUD Gp38 OS=Stenotrophomonas phage S1 OX=573591 PE=4 SV=1
MM1 pKa = 7.59 SARR4 pKa = 11.84 PQQIGRR10 pKa = 11.84 AAEE13 pKa = 3.95 VRR15 pKa = 11.84 KK16 pKa = 9.65 VLSMFPQGATVEE28 pKa = 4.3 QIKK31 pKa = 8.11 TAGRR35 pKa = 11.84 INSTHH40 pKa = 5.1 QAIGYY45 pKa = 4.99 TLKK48 pKa = 10.85 GLARR52 pKa = 11.84 SGQAICHH59 pKa = 6.1 RR60 pKa = 11.84 SGVRR64 pKa = 11.84 GIWRR68 pKa = 11.84 LSSHH72 pKa = 5.46 TQHH75 pKa = 7.27 AIAPLRR81 pKa = 11.84 AAPARR86 pKa = 11.84 EE87 pKa = 3.88 RR88 pKa = 11.84 PTCVVGPLTGVSDD101 pKa = 3.78 AATTIRR107 pKa = 11.84 HH108 pKa = 6.0 RR109 pKa = 11.84 EE110 pKa = 3.77 LDD112 pKa = 3.81 RR113 pKa = 11.84 QQLADD118 pKa = 4.64 DD119 pKa = 5.0 LDD121 pKa = 4.41 AFLAAGGHH129 pKa = 5.91 IEE131 pKa = 4.38 VLGHH135 pKa = 5.52 TPLRR139 pKa = 11.84 PLMSRR144 pKa = 11.84 HH145 pKa = 4.86 TANHH149 pKa = 5.53 GSYY152 pKa = 10.42 AEE154 pKa = 3.99 RR155 pKa = 11.84 MAAHH159 pKa = 6.79 EE160 pKa = 4.17 MDD162 pKa = 3.44
Molecular weight: 17.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.414
IPC_protein 10.423
Toseland 10.54
ProMoST 10.365
Dawson 10.643
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.643
Grimsley 10.701
Solomon 10.818
Lehninger 10.774
Nozaki 10.555
DTASelect 10.409
Thurlkill 10.555
EMBOSS 10.965
Sillero 10.584
Patrickios 10.423
IPC_peptide 10.818
IPC2_peptide 9.706
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
12930
69
1248
269.4
29.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.908 ± 0.769
1.114 ± 0.216
5.731 ± 0.255
5.352 ± 0.346
2.56 ± 0.176
8.268 ± 0.356
1.91 ± 0.207
3.859 ± 0.221
3.155 ± 0.29
8.577 ± 0.288
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.196 ± 0.138
2.738 ± 0.165
5.391 ± 0.454
4.47 ± 0.362
8.484 ± 0.471
6.551 ± 0.373
5.53 ± 0.319
7.108 ± 0.374
1.895 ± 0.158
2.204 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here