Amino acid dipepetide frequency for Stenotrophomonas phage S1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.872AlaAla: 18.872 ± 3.185
1.005AlaCys: 1.005 ± 0.29
8.044AlaAsp: 8.044 ± 0.86
6.961AlaGlu: 6.961 ± 0.925
3.326AlaPhe: 3.326 ± 0.565
11.834AlaGly: 11.834 ± 1.206
2.011AlaHis: 2.011 ± 0.525
4.486AlaIle: 4.486 ± 0.73
4.099AlaLys: 4.099 ± 0.715
9.746AlaLeu: 9.746 ± 0.873
3.481AlaMet: 3.481 ± 0.611
3.171AlaAsn: 3.171 ± 0.491
6.42AlaPro: 6.42 ± 0.721
6.11AlaGln: 6.11 ± 1.15
9.436AlaArg: 9.436 ± 1.179
7.812AlaSer: 7.812 ± 1.113
8.199AlaThr: 8.199 ± 0.934
8.431AlaVal: 8.431 ± 0.694
2.862AlaTrp: 2.862 ± 0.471
2.784AlaTyr: 2.784 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
1.238CysAla: 1.238 ± 0.387
0.464CysCys: 0.464 ± 0.244
0.696CysAsp: 0.696 ± 0.251
0.387CysGlu: 0.387 ± 0.165
0.077CysPhe: 0.077 ± 0.073
1.47CysGly: 1.47 ± 0.44
0.387CysHis: 0.387 ± 0.168
0.387CysIle: 0.387 ± 0.171
0.464CysLys: 0.464 ± 0.227
0.851CysLeu: 0.851 ± 0.265
0.387CysMet: 0.387 ± 0.168
0.232CysAsn: 0.232 ± 0.113
0.541CysPro: 0.541 ± 0.176
0.0CysGln: 0.0 ± 0.0
0.851CysArg: 0.851 ± 0.297
1.005CysSer: 1.005 ± 0.347
0.464CysThr: 0.464 ± 0.212
0.928CysVal: 0.928 ± 0.317
0.0CysTrp: 0.0 ± 0.0
0.309CysTyr: 0.309 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
7.348AspAla: 7.348 ± 0.671
0.464AspCys: 0.464 ± 0.194
3.249AspAsp: 3.249 ± 0.532
3.171AspGlu: 3.171 ± 0.514
1.238AspPhe: 1.238 ± 0.327
5.492AspGly: 5.492 ± 0.615
1.16AspHis: 1.16 ± 0.263
2.707AspIle: 2.707 ± 0.599
1.083AspLys: 1.083 ± 0.303
5.956AspLeu: 5.956 ± 0.629
1.16AspMet: 1.16 ± 0.247
1.392AspAsn: 1.392 ± 0.291
2.939AspPro: 2.939 ± 0.454
1.779AspGln: 1.779 ± 0.293
5.027AspArg: 5.027 ± 0.777
3.558AspSer: 3.558 ± 0.495
2.63AspThr: 2.63 ± 0.414
3.79AspVal: 3.79 ± 0.398
1.47AspTrp: 1.47 ± 0.32
1.702AspTyr: 1.702 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
7.503GluAla: 7.503 ± 1.092
0.541GluCys: 0.541 ± 0.215
2.552GluAsp: 2.552 ± 0.449
3.016GluGlu: 3.016 ± 0.458
1.315GluPhe: 1.315 ± 0.262
3.635GluGly: 3.635 ± 0.569
1.005GluHis: 1.005 ± 0.325
1.856GluIle: 1.856 ± 0.361
1.238GluLys: 1.238 ± 0.308
6.033GluLeu: 6.033 ± 0.796
0.851GluMet: 0.851 ± 0.26
1.083GluAsn: 1.083 ± 0.298
2.475GluPro: 2.475 ± 0.473
2.707GluGln: 2.707 ± 0.831
4.641GluArg: 4.641 ± 0.596
3.481GluSer: 3.481 ± 0.483
2.707GluThr: 2.707 ± 0.402
5.105GluVal: 5.105 ± 0.572
0.851GluTrp: 0.851 ± 0.256
0.928GluTyr: 0.928 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.364
0.232PheCys: 0.232 ± 0.13
1.702PheAsp: 1.702 ± 0.384
1.392PheGlu: 1.392 ± 0.364
0.696PhePhe: 0.696 ± 0.224
2.475PheGly: 2.475 ± 0.438
0.309PheHis: 0.309 ± 0.196
1.005PheIle: 1.005 ± 0.266
1.16PheLys: 1.16 ± 0.378
2.011PheLeu: 2.011 ± 0.454
0.309PheMet: 0.309 ± 0.14
0.619PheAsn: 0.619 ± 0.233
1.856PhePro: 1.856 ± 0.412
0.696PheGln: 0.696 ± 0.247
2.011PheArg: 2.011 ± 0.317
2.475PheSer: 2.475 ± 0.589
1.856PheThr: 1.856 ± 0.352
1.702PheVal: 1.702 ± 0.315
0.155PheTrp: 0.155 ± 0.116
0.464PheTyr: 0.464 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
8.431GlyAla: 8.431 ± 0.979
1.083GlyCys: 1.083 ± 0.415
4.563GlyAsp: 4.563 ± 0.605
5.569GlyGlu: 5.569 ± 0.655
2.166GlyPhe: 2.166 ± 0.453
6.884GlyGly: 6.884 ± 1.022
1.547GlyHis: 1.547 ± 0.327
3.635GlyIle: 3.635 ± 0.55
3.016GlyLys: 3.016 ± 0.443
7.116GlyLeu: 7.116 ± 0.609
2.011GlyMet: 2.011 ± 0.37
2.707GlyAsn: 2.707 ± 0.492
3.016GlyPro: 3.016 ± 0.459
4.099GlyGln: 4.099 ± 0.587
7.038GlyArg: 7.038 ± 0.608
6.033GlySer: 6.033 ± 1.283
4.795GlyThr: 4.795 ± 0.797
5.724GlyVal: 5.724 ± 0.615
1.392GlyTrp: 1.392 ± 0.308
1.856GlyTyr: 1.856 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
2.862HisAla: 2.862 ± 0.472
0.309HisCys: 0.309 ± 0.142
0.851HisAsp: 0.851 ± 0.258
0.773HisGlu: 0.773 ± 0.25
0.619HisPhe: 0.619 ± 0.212
2.011HisGly: 2.011 ± 0.473
0.696HisHis: 0.696 ± 0.256
0.541HisIle: 0.541 ± 0.219
0.619HisLys: 0.619 ± 0.219
1.547HisLeu: 1.547 ± 0.413
0.696HisMet: 0.696 ± 0.235
0.464HisAsn: 0.464 ± 0.205
1.238HisPro: 1.238 ± 0.439
0.464HisGln: 0.464 ± 0.164
2.011HisArg: 2.011 ± 0.452
1.005HisSer: 1.005 ± 0.295
0.619HisThr: 0.619 ± 0.274
1.005HisVal: 1.005 ± 0.302
0.387HisTrp: 0.387 ± 0.224
0.309HisTyr: 0.309 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
5.105IleAla: 5.105 ± 0.634
0.077IleCys: 0.077 ± 0.071
2.939IleAsp: 2.939 ± 0.525
3.403IleGlu: 3.403 ± 0.413
0.696IlePhe: 0.696 ± 0.203
3.79IleGly: 3.79 ± 0.483
0.773IleHis: 0.773 ± 0.253
1.702IleIle: 1.702 ± 0.385
1.315IleLys: 1.315 ± 0.28
1.856IleLeu: 1.856 ± 0.36
0.387IleMet: 0.387 ± 0.187
2.011IleAsn: 2.011 ± 0.435
1.624IlePro: 1.624 ± 0.402
1.315IleGln: 1.315 ± 0.271
2.707IleArg: 2.707 ± 0.476
3.326IleSer: 3.326 ± 0.752
2.784IleThr: 2.784 ± 0.505
1.624IleVal: 1.624 ± 0.428
0.541IleTrp: 0.541 ± 0.197
0.619IleTyr: 0.619 ± 0.171
0.0IleXaa: 0.0 ± 0.0
Lys
4.718LysAla: 4.718 ± 1.351
0.232LysCys: 0.232 ± 0.129
1.624LysAsp: 1.624 ± 0.29
1.934LysGlu: 1.934 ± 0.526
0.619LysPhe: 0.619 ± 0.257
2.243LysGly: 2.243 ± 0.438
0.696LysHis: 0.696 ± 0.259
0.619LysIle: 0.619 ± 0.209
1.315LysLys: 1.315 ± 0.315
2.862LysLeu: 2.862 ± 0.507
1.005LysMet: 1.005 ± 0.31
0.928LysAsn: 0.928 ± 0.297
2.32LysPro: 2.32 ± 0.405
1.392LysGln: 1.392 ± 0.484
2.862LysArg: 2.862 ± 0.459
1.547LysSer: 1.547 ± 0.436
1.702LysThr: 1.702 ± 0.347
1.856LysVal: 1.856 ± 0.258
0.541LysTrp: 0.541 ± 0.178
0.541LysTyr: 0.541 ± 0.228
0.0LysXaa: 0.0 ± 0.0
Leu
12.53LeuAla: 12.53 ± 1.256
0.851LeuCys: 0.851 ± 0.272
5.724LeuAsp: 5.724 ± 0.621
3.403LeuGlu: 3.403 ± 0.736
1.702LeuPhe: 1.702 ± 0.35
6.188LeuGly: 6.188 ± 0.803
2.398LeuHis: 2.398 ± 0.659
3.171LeuIle: 3.171 ± 0.551
2.475LeuLys: 2.475 ± 0.313
6.574LeuLeu: 6.574 ± 0.804
1.856LeuMet: 1.856 ± 0.404
2.32LeuAsn: 2.32 ± 0.612
4.177LeuPro: 4.177 ± 0.889
5.027LeuGln: 5.027 ± 0.561
7.503LeuArg: 7.503 ± 0.859
5.724LeuSer: 5.724 ± 0.789
4.795LeuThr: 4.795 ± 0.675
5.801LeuVal: 5.801 ± 0.903
1.083LeuTrp: 1.083 ± 0.314
2.475LeuTyr: 2.475 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
2.552MetAla: 2.552 ± 0.349
0.541MetCys: 0.541 ± 0.185
0.773MetAsp: 0.773 ± 0.248
1.856MetGlu: 1.856 ± 0.436
0.851MetPhe: 0.851 ± 0.223
1.624MetGly: 1.624 ± 0.325
0.232MetHis: 0.232 ± 0.123
0.464MetIle: 0.464 ± 0.198
0.851MetLys: 0.851 ± 0.264
1.315MetLeu: 1.315 ± 0.321
0.232MetMet: 0.232 ± 0.163
0.464MetAsn: 0.464 ± 0.179
1.779MetPro: 1.779 ± 0.395
1.392MetGln: 1.392 ± 0.331
2.398MetArg: 2.398 ± 0.442
1.547MetSer: 1.547 ± 0.365
1.47MetThr: 1.47 ± 0.316
1.238MetVal: 1.238 ± 0.324
0.232MetTrp: 0.232 ± 0.138
0.155MetTyr: 0.155 ± 0.081
0.0MetXaa: 0.0 ± 0.0
Asn
3.403AsnAla: 3.403 ± 0.539
0.619AsnCys: 0.619 ± 0.21
1.702AsnAsp: 1.702 ± 0.494
1.005AsnGlu: 1.005 ± 0.271
0.619AsnPhe: 0.619 ± 0.252
3.016AsnGly: 3.016 ± 0.533
0.387AsnHis: 0.387 ± 0.134
1.005AsnIle: 1.005 ± 0.297
0.928AsnLys: 0.928 ± 0.301
2.398AsnLeu: 2.398 ± 0.532
0.541AsnMet: 0.541 ± 0.178
1.16AsnAsn: 1.16 ± 0.329
2.552AsnPro: 2.552 ± 0.386
1.083AsnGln: 1.083 ± 0.3
2.243AsnArg: 2.243 ± 0.417
0.928AsnSer: 0.928 ± 0.228
1.47AsnThr: 1.47 ± 0.331
1.547AsnVal: 1.547 ± 0.273
0.155AsnTrp: 0.155 ± 0.175
0.619AsnTyr: 0.619 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
6.497ProAla: 6.497 ± 1.01
1.005ProCys: 1.005 ± 0.397
3.403ProAsp: 3.403 ± 0.488
1.779ProGlu: 1.779 ± 0.459
1.083ProPhe: 1.083 ± 0.323
5.105ProGly: 5.105 ± 0.941
0.928ProHis: 0.928 ± 0.366
2.088ProIle: 2.088 ± 0.353
1.392ProLys: 1.392 ± 0.351
5.182ProLeu: 5.182 ± 0.852
1.547ProMet: 1.547 ± 0.413
1.083ProAsn: 1.083 ± 0.239
2.707ProPro: 2.707 ± 0.577
2.939ProGln: 2.939 ± 0.435
3.558ProArg: 3.558 ± 0.81
4.254ProSer: 4.254 ± 0.603
2.398ProThr: 2.398 ± 0.448
4.486ProVal: 4.486 ± 0.609
1.47ProTrp: 1.47 ± 0.469
1.005ProTyr: 1.005 ± 0.342
0.0ProXaa: 0.0 ± 0.0
Gln
6.033GlnAla: 6.033 ± 0.781
0.232GlnCys: 0.232 ± 0.151
1.702GlnAsp: 1.702 ± 0.374
2.088GlnGlu: 2.088 ± 0.386
1.47GlnPhe: 1.47 ± 0.274
3.171GlnGly: 3.171 ± 0.506
0.851GlnHis: 0.851 ± 0.287
2.011GlnIle: 2.011 ± 0.41
1.702GlnLys: 1.702 ± 0.422
4.331GlnLeu: 4.331 ± 0.841
1.47GlnMet: 1.47 ± 0.393
0.541GlnAsn: 0.541 ± 0.188
2.707GlnPro: 2.707 ± 0.657
3.016GlnGln: 3.016 ± 0.842
3.635GlnArg: 3.635 ± 0.604
2.939GlnSer: 2.939 ± 0.473
1.315GlnThr: 1.315 ± 0.283
3.558GlnVal: 3.558 ± 0.446
1.083GlnTrp: 1.083 ± 0.241
0.851GlnTyr: 0.851 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
10.21ArgAla: 10.21 ± 1.305
1.315ArgCys: 1.315 ± 0.394
3.635ArgAsp: 3.635 ± 0.453
4.563ArgGlu: 4.563 ± 0.724
2.707ArgPhe: 2.707 ± 0.391
5.182ArgGly: 5.182 ± 0.604
2.088ArgHis: 2.088 ± 0.443
3.713ArgIle: 3.713 ± 0.469
3.171ArgLys: 3.171 ± 0.527
8.508ArgLeu: 8.508 ± 0.7
1.856ArgMet: 1.856 ± 0.316
2.243ArgAsn: 2.243 ± 0.396
4.718ArgPro: 4.718 ± 1.062
3.481ArgGln: 3.481 ± 0.502
7.812ArgArg: 7.812 ± 1.324
5.801ArgSer: 5.801 ± 0.995
4.563ArgThr: 4.563 ± 0.527
5.337ArgVal: 5.337 ± 0.614
2.088ArgTrp: 2.088 ± 0.449
1.856ArgTyr: 1.856 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
9.436SerAla: 9.436 ± 0.761
0.464SerCys: 0.464 ± 0.225
4.177SerAsp: 4.177 ± 0.577
2.243SerGlu: 2.243 ± 0.339
1.779SerPhe: 1.779 ± 0.327
5.878SerGly: 5.878 ± 0.752
1.083SerHis: 1.083 ± 0.232
2.32SerIle: 2.32 ± 0.517
1.934SerLys: 1.934 ± 0.382
5.801SerLeu: 5.801 ± 0.972
1.47SerMet: 1.47 ± 0.301
2.011SerAsn: 2.011 ± 0.363
2.784SerPro: 2.784 ± 0.511
3.171SerGln: 3.171 ± 0.405
5.337SerArg: 5.337 ± 0.785
3.867SerSer: 3.867 ± 0.589
3.171SerThr: 3.171 ± 0.509
6.11SerVal: 6.11 ± 1.029
1.005SerTrp: 1.005 ± 0.273
1.47SerTyr: 1.47 ± 0.376
0.0SerXaa: 0.0 ± 0.0
Thr
7.657ThrAla: 7.657 ± 0.537
0.541ThrCys: 0.541 ± 0.188
3.558ThrAsp: 3.558 ± 0.665
3.016ThrGlu: 3.016 ± 0.457
1.624ThrPhe: 1.624 ± 0.389
5.492ThrGly: 5.492 ± 0.79
0.619ThrHis: 0.619 ± 0.184
2.939ThrIle: 2.939 ± 0.452
1.16ThrLys: 1.16 ± 0.311
4.563ThrLeu: 4.563 ± 0.622
0.851ThrMet: 0.851 ± 0.289
1.47ThrAsn: 1.47 ± 0.349
3.249ThrPro: 3.249 ± 0.476
1.779ThrGln: 1.779 ± 0.409
3.403ThrArg: 3.403 ± 0.495
3.326ThrSer: 3.326 ± 0.509
3.713ThrThr: 3.713 ± 0.532
3.79ThrVal: 3.79 ± 0.583
1.083ThrTrp: 1.083 ± 0.402
1.47ThrTyr: 1.47 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
8.044ValAla: 8.044 ± 0.915
0.619ValCys: 0.619 ± 0.242
3.635ValAsp: 3.635 ± 0.678
4.409ValGlu: 4.409 ± 0.432
2.011ValPhe: 2.011 ± 0.552
4.486ValGly: 4.486 ± 0.612
0.928ValHis: 0.928 ± 0.269
2.784ValIle: 2.784 ± 0.458
2.707ValLys: 2.707 ± 0.529
5.414ValLeu: 5.414 ± 0.824
0.696ValMet: 0.696 ± 0.221
1.856ValAsn: 1.856 ± 0.3
4.718ValPro: 4.718 ± 0.704
2.707ValGln: 2.707 ± 0.344
8.276ValArg: 8.276 ± 0.866
4.641ValSer: 4.641 ± 0.556
4.022ValThr: 4.022 ± 0.578
6.033ValVal: 6.033 ± 1.059
1.624ValTrp: 1.624 ± 0.338
1.47ValTyr: 1.47 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.624TrpAla: 1.624 ± 0.336
0.309TrpCys: 0.309 ± 0.128
1.083TrpAsp: 1.083 ± 0.369
1.16TrpGlu: 1.16 ± 0.206
0.696TrpPhe: 0.696 ± 0.178
1.083TrpGly: 1.083 ± 0.411
0.387TrpHis: 0.387 ± 0.19
0.541TrpIle: 0.541 ± 0.145
0.696TrpLys: 0.696 ± 0.271
1.934TrpLeu: 1.934 ± 0.324
0.696TrpMet: 0.696 ± 0.218
0.541TrpAsn: 0.541 ± 0.188
0.773TrpPro: 0.773 ± 0.277
0.696TrpGln: 0.696 ± 0.296
2.166TrpArg: 2.166 ± 0.412
1.083TrpSer: 1.083 ± 0.25
1.16TrpThr: 1.16 ± 0.283
1.392TrpVal: 1.392 ± 0.321
0.387TrpTrp: 0.387 ± 0.226
0.541TrpTyr: 0.541 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.552TyrAla: 2.552 ± 0.406
0.232TyrCys: 0.232 ± 0.168
1.702TyrAsp: 1.702 ± 0.266
1.392TyrGlu: 1.392 ± 0.308
0.309TyrPhe: 0.309 ± 0.125
1.624TyrGly: 1.624 ± 0.506
0.619TyrHis: 0.619 ± 0.204
0.619TyrIle: 0.619 ± 0.163
0.232TyrLys: 0.232 ± 0.12
1.779TyrLeu: 1.779 ± 0.4
0.387TyrMet: 0.387 ± 0.173
1.083TyrAsn: 1.083 ± 0.296
1.315TyrPro: 1.315 ± 0.372
0.851TyrGln: 0.851 ± 0.238
1.934TyrArg: 1.934 ± 0.381
1.16TyrSer: 1.16 ± 0.287
1.47TyrThr: 1.47 ± 0.395
1.624TyrVal: 1.624 ± 0.337
0.541TyrTrp: 0.541 ± 0.193
0.619TyrTyr: 0.619 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12930 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski