Aliterella atlantica CENA595
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4366 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D8ZV76|A0A0D8ZV76_9CYAN Glutamate synthase OS=Aliterella atlantica CENA595 OX=1618023 GN=UH38_05865 PE=3 SV=1
MM1 pKa = 6.99 TVNVYY6 pKa = 7.69 ATPAYY11 pKa = 9.59 IQILLIDD18 pKa = 3.91 ILIKK22 pKa = 8.18 EE23 pKa = 4.53 TKK25 pKa = 10.52 VFDD28 pKa = 4.23 EE29 pKa = 5.02 LDD31 pKa = 3.45 SDD33 pKa = 4.94 LVPEE37 pKa = 4.72 EE38 pKa = 4.77 CPSCGSNHH46 pKa = 6.31 TYY48 pKa = 10.36 FDD50 pKa = 3.56 RR51 pKa = 11.84 AFNFHH56 pKa = 6.51 KK57 pKa = 11.12 CEE59 pKa = 4.4 DD60 pKa = 4.11 CSLMWGFDD68 pKa = 3.69 SDD70 pKa = 5.21 DD71 pKa = 3.7 PDD73 pKa = 3.91 YY74 pKa = 11.81 DD75 pKa = 3.7 EE76 pKa = 6.62 DD77 pKa = 5.06 LYY79 pKa = 11.6 EE80 pKa = 5.75 DD81 pKa = 3.61 MKK83 pKa = 11.08 ADD85 pKa = 4.23 QMAEE89 pKa = 3.92 NLKK92 pKa = 10.96 DD93 pKa = 4.22 PIEE96 pKa = 4.47 DD97 pKa = 3.59 FF98 pKa = 4.34
Molecular weight: 11.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.846
Patrickios 0.655
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A0D8ZT21|A0A0D8ZT21_9CYAN Ribosome biogenesis GTPase A OS=Aliterella atlantica CENA595 OX=1618023 GN=UH38_09240 PE=3 SV=1
MM1 pKa = 7.53 VVILSCIPLRR11 pKa = 11.84 MAIAKK16 pKa = 9.58 QIAPAPQAILTLGGDD31 pKa = 3.35 IDD33 pKa = 5.19 RR34 pKa = 11.84 EE35 pKa = 4.0 IFTARR40 pKa = 11.84 FAQMHH45 pKa = 6.82 PSLDD49 pKa = 2.96 IWISSGANLEE59 pKa = 3.88 ATRR62 pKa = 11.84 RR63 pKa = 11.84 AFQQIAPTRR72 pKa = 11.84 VHH74 pKa = 6.43 IDD76 pKa = 2.69 RR77 pKa = 11.84 RR78 pKa = 11.84 AIDD81 pKa = 3.5 TVTNFTSLVADD92 pKa = 5.15 FKK94 pKa = 9.62 QHH96 pKa = 6.18 KK97 pKa = 8.4 FHH99 pKa = 7.19 HH100 pKa = 6.73 IYY102 pKa = 10.77 VVTADD107 pKa = 3.29 YY108 pKa = 11.34 HH109 pKa = 4.47 MRR111 pKa = 11.84 RR112 pKa = 11.84 AKK114 pKa = 10.6 AIATFVLGSQGIAFTPVAMPTNKK137 pKa = 9.49 PVEE140 pKa = 4.48 SRR142 pKa = 11.84 LHH144 pKa = 6.16 LLRR147 pKa = 11.84 DD148 pKa = 3.44 VCRR151 pKa = 11.84 ALLWVLTRR159 pKa = 11.84 RR160 pKa = 11.84 TGASLHH166 pKa = 6.14 PSII169 pKa = 5.69
Molecular weight: 18.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.56
IPC_protein 10.482
Toseland 10.76
ProMoST 10.526
Dawson 10.818
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 10.935
Grimsley 10.862
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.169
Sillero 10.774
Patrickios 10.701
IPC_peptide 11.008
IPC2_peptide 9.736
IPC2.peptide.svr19 8.674
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4366
0
4366
1296007
31
2198
296.8
32.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.015 ± 0.043
1.017 ± 0.013
4.94 ± 0.027
5.973 ± 0.039
3.787 ± 0.025
6.632 ± 0.037
1.751 ± 0.016
6.583 ± 0.03
4.565 ± 0.032
10.94 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.017
4.147 ± 0.027
4.716 ± 0.033
5.493 ± 0.037
5.324 ± 0.028
6.307 ± 0.032
5.589 ± 0.03
6.872 ± 0.027
1.422 ± 0.018
3.058 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here