Pseudoalteromonas rubra

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4390 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4QPL9|A0A0F4QPL9_9GAMM Bacterioferritin OS=Pseudoalteromonas rubra OX=43658 GN=bfr PE=3 SV=1
MM1 pKa = 8.15DD2 pKa = 4.58YY3 pKa = 9.86STSDD7 pKa = 3.47LCDD10 pKa = 3.4HH11 pKa = 6.92FADD14 pKa = 4.11AVDD17 pKa = 3.54VLEE20 pKa = 4.59PMFINFGGQSAFSGRR35 pKa = 11.84IKK37 pKa = 9.73TLKK40 pKa = 10.25CFEE43 pKa = 4.42NNEE46 pKa = 4.71LINQVLQQDD55 pKa = 3.9GTGQVLLVDD64 pKa = 4.63GGGSTRR70 pKa = 11.84RR71 pKa = 11.84ALIDD75 pKa = 3.32IEE77 pKa = 4.1LAEE80 pKa = 4.94LAIEE84 pKa = 4.36NNWQGIVVYY93 pKa = 10.01GAVRR97 pKa = 11.84HH98 pKa = 4.91VEE100 pKa = 3.96EE101 pKa = 6.22LEE103 pKa = 4.05EE104 pKa = 5.45CDD106 pKa = 5.7LGVQAIASIPVAADD120 pKa = 3.06SHH122 pKa = 6.74GSGEE126 pKa = 3.9QGIPVNFAGVSFYY139 pKa = 11.31DD140 pKa = 4.49DD141 pKa = 4.15DD142 pKa = 4.23YY143 pKa = 11.77LYY145 pKa = 11.4ADD147 pKa = 3.58ATGIIISAEE156 pKa = 3.96EE157 pKa = 3.96LLLEE161 pKa = 4.43VDD163 pKa = 4.69DD164 pKa = 4.83EE165 pKa = 4.36QGEE168 pKa = 4.24

Molecular weight: 18.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4QL14|A0A0F4QL14_9GAMM Uncharacterized protein OS=Pseudoalteromonas rubra OX=43658 GN=TW77_13870 PE=4 SV=1
MM1 pKa = 7.57GSDD4 pKa = 3.52NSPALSLGEE13 pKa = 3.94PLQVCDD19 pKa = 4.09KK20 pKa = 9.45QRR22 pKa = 11.84KK23 pKa = 7.32RR24 pKa = 11.84AVRR27 pKa = 11.84KK28 pKa = 9.07RR29 pKa = 11.84WILTGSTSAVLKK41 pKa = 10.71QLTLPMLWAILALFSADD58 pKa = 4.51LIEE61 pKa = 5.65LYY63 pKa = 10.22FASRR67 pKa = 11.84LGVEE71 pKa = 4.71EE72 pKa = 4.12LTAMSFTLPVQATLFAFAIGLGIVVATRR100 pKa = 11.84LTQAKK105 pKa = 9.27EE106 pKa = 4.14VEE108 pKa = 4.12QLAAVSLIFTVLVGATLAAVIWLGLQPLLTLLGFAEE144 pKa = 5.1FAARR148 pKa = 11.84EE149 pKa = 4.25QVWPTLEE156 pKa = 5.32RR157 pKa = 11.84YY158 pKa = 6.77MQYY161 pKa = 11.22RR162 pKa = 11.84LGAVVFFFIVMVVFGVLRR180 pKa = 11.84AFGNMRR186 pKa = 11.84GAAQLLVTFAGLQIVISAALFTPWAHH212 pKa = 6.27SALPLSGLEE221 pKa = 4.05RR222 pKa = 11.84LGVAHH227 pKa = 7.12FIAAVSASLYY237 pKa = 10.92ALYY240 pKa = 10.83LLRR243 pKa = 11.84IKK245 pKa = 10.68EE246 pKa = 4.19NISLKK251 pKa = 10.33VRR253 pKa = 11.84LLSQRR258 pKa = 11.84SRR260 pKa = 11.84QAFRR264 pKa = 11.84RR265 pKa = 11.84LFRR268 pKa = 11.84LFVPVVAMQLLTPLAQSLLMMIVASQGSDD297 pKa = 2.62AVAAFGVVMRR307 pKa = 11.84IEE309 pKa = 4.23PLALLLPMVLTTSLPIFVGQNWAANKK335 pKa = 9.41SLRR338 pKa = 11.84VRR340 pKa = 11.84RR341 pKa = 11.84GIKK344 pKa = 9.38QAIAACLVWQSVIALVLFWGADD366 pKa = 3.23MLGVGFCKK374 pKa = 10.35QSFVSQSIEE383 pKa = 3.56LAMCILPVSYY393 pKa = 10.54AALAGVMLYY402 pKa = 9.68VSCCNAIGRR411 pKa = 11.84SALALNVTLVRR422 pKa = 11.84LFVLSLPCAYY432 pKa = 10.52LGAQLNGFQGIVWALALANFVVGAWLLFRR461 pKa = 11.84ALNVQRR467 pKa = 11.84RR468 pKa = 11.84PQVLTMSS475 pKa = 4.17

Molecular weight: 51.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4390

0

4390

1545420

37

6585

352.0

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.37 ± 0.039

1.061 ± 0.012

5.632 ± 0.032

6.011 ± 0.032

4.07 ± 0.025

6.636 ± 0.035

2.432 ± 0.022

5.587 ± 0.029

4.804 ± 0.031

10.753 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.019

4.064 ± 0.027

3.941 ± 0.025

5.385 ± 0.039

4.553 ± 0.031

6.633 ± 0.042

5.428 ± 0.037

6.861 ± 0.032

1.214 ± 0.015

3.243 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski