Genlisea aurea
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17675 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S8DA88|S8DA88_9LAMI CASP-like protein (Fragment) OS=Genlisea aurea OX=192259 GN=M569_00354 PE=3 SV=1
MM1 pKa = 7.78 GSDD4 pKa = 3.17 TRR6 pKa = 11.84 DD7 pKa = 3.32 GEE9 pKa = 4.51 DD10 pKa = 3.43 YY11 pKa = 10.46 FVKK14 pKa = 10.56 VVAGEE19 pKa = 4.13 FGYY22 pKa = 10.88 VLQDD26 pKa = 3.4 VPHH29 pKa = 6.19 LTDD32 pKa = 4.74 YY33 pKa = 11.22 IPDD36 pKa = 3.47 LTTYY40 pKa = 9.94 PNPLGSNSAYY50 pKa = 10.63 SVVKK54 pKa = 10.38 QYY56 pKa = 10.82 FVDD59 pKa = 3.64 MDD61 pKa = 4.04 DD62 pKa = 4.21 SVPEE66 pKa = 4.32 KK67 pKa = 10.52 IVLLLLLII75 pKa = 4.67
Molecular weight: 8.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 0.782
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|S8CQP1|S8CQP1_9LAMI H/ACA ribonucleoprotein complex subunit (Fragment) OS=Genlisea aurea OX=192259 GN=M569_05695 PE=3 SV=1
RR1 pKa = 7.64 RR2 pKa = 11.84 ISSRR6 pKa = 11.84 RR7 pKa = 11.84 DD8 pKa = 2.81 MSRR11 pKa = 11.84 RR12 pKa = 11.84 GRR14 pKa = 11.84 RR15 pKa = 11.84 GARR18 pKa = 11.84 RR19 pKa = 11.84 KK20 pKa = 9.16 RR21 pKa = 11.84 NRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 GRR34 pKa = 11.84 GRR36 pKa = 11.84 NRR38 pKa = 11.84 SVLGSNLGNRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 GRR52 pKa = 11.84 RR53 pKa = 11.84 IRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 VEE60 pKa = 3.71 FRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 PFFRR68 pKa = 11.84 NRR70 pKa = 2.93
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.574
Grimsley 13.1
Solomon 13.554
Lehninger 13.451
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.281
IPC_peptide 13.554
IPC2_peptide 12.544
IPC2.peptide.svr19 9.242
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17675
0
17675
5688215
50
5020
321.8
35.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.399 ± 0.016
1.813 ± 0.01
5.484 ± 0.012
6.51 ± 0.021
4.351 ± 0.012
6.876 ± 0.019
2.297 ± 0.008
5.314 ± 0.014
5.663 ± 0.017
9.704 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.008
3.96 ± 0.01
4.818 ± 0.018
3.254 ± 0.01
6.019 ± 0.016
8.747 ± 0.021
4.53 ± 0.01
6.833 ± 0.014
1.326 ± 0.007
2.847 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here