Tilligerry virus
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9ZXR0|H9ZXR0_9REOV VP6 OS=Tilligerry virus OX=1170505 PE=4 SV=1
MM1 pKa = 7.33 EE2 pKa = 5.14 SKK4 pKa = 10.2 VVKK7 pKa = 10.25 RR8 pKa = 11.84 VFTKK12 pKa = 9.58 QICVLDD18 pKa = 4.48 PGCSTICGKK27 pKa = 9.87 IARR30 pKa = 11.84 EE31 pKa = 4.19 SKK33 pKa = 10.79 CPYY36 pKa = 8.44 TQLKK40 pKa = 7.73 TGRR43 pKa = 11.84 IVGFQAVRR51 pKa = 11.84 SPPPKK56 pKa = 10.39 AYY58 pKa = 9.43 ILEE61 pKa = 4.16 ITKK64 pKa = 10.09 PGAYY68 pKa = 9.29 RR69 pKa = 11.84 IMDD72 pKa = 4.02 GQDD75 pKa = 3.02 QISIMVEE82 pKa = 3.94 EE83 pKa = 4.6 YY84 pKa = 10.71 GVEE87 pKa = 3.66 ITTDD91 pKa = 2.93 RR92 pKa = 11.84 WEE94 pKa = 3.76 EE95 pKa = 3.5 WKK97 pKa = 11.19 YY98 pKa = 10.99 EE99 pKa = 4.19 VVTAMPMTALVDD111 pKa = 3.87 TPRR114 pKa = 11.84 GKK116 pKa = 10.46 VDD118 pKa = 4.29 AEE120 pKa = 3.93 LKK122 pKa = 8.81 YY123 pKa = 11.12 ARR125 pKa = 11.84 GTGLVPAYY133 pKa = 8.92 TRR135 pKa = 11.84 NEE137 pKa = 3.66 FDD139 pKa = 3.64 RR140 pKa = 11.84 RR141 pKa = 11.84 EE142 pKa = 4.19 LPTLPGVGVSEE153 pKa = 4.04 ISVRR157 pKa = 11.84 DD158 pKa = 3.42 LRR160 pKa = 11.84 AKK162 pKa = 10.57 LKK164 pKa = 10.82 AEE166 pKa = 3.92 RR167 pKa = 11.84 TADD170 pKa = 3.17 IPRR173 pKa = 11.84 AEE175 pKa = 4.36 HH176 pKa = 7.13 KK177 pKa = 10.39 EE178 pKa = 3.99 EE179 pKa = 3.79 KK180 pKa = 10.04 KK181 pKa = 11.0 KK182 pKa = 10.64 NVRR185 pKa = 11.84 EE186 pKa = 4.07 NTSVFDD192 pKa = 3.69 VSEE195 pKa = 3.98 MLKK198 pKa = 10.29 KK199 pKa = 10.09 VEE201 pKa = 4.04 EE202 pKa = 4.13 MRR204 pKa = 11.84 SEE206 pKa = 3.93 EE207 pKa = 4.09 RR208 pKa = 11.84 SWSDD212 pKa = 3.09 EE213 pKa = 3.94 MEE215 pKa = 4.79 DD216 pKa = 3.82 EE217 pKa = 4.85 VEE219 pKa = 4.99 GEE221 pKa = 3.86 AADD224 pKa = 4.43 VNEE227 pKa = 4.22 QTDD230 pKa = 4.12 ALWEE234 pKa = 4.36 DD235 pKa = 4.15 EE236 pKa = 4.28 VTEE239 pKa = 4.6 SVEE242 pKa = 4.38 EE243 pKa = 3.85 PDD245 pKa = 4.05 KK246 pKa = 11.9 ANLEE250 pKa = 4.68 DD251 pKa = 3.5 IAEE254 pKa = 4.16 EE255 pKa = 4.16 EE256 pKa = 4.53 EE257 pKa = 4.51 PFNATNFVTDD267 pKa = 3.82 GFVANVSTYY276 pKa = 9.36 MKK278 pKa = 10.46 SGDD281 pKa = 3.43 KK282 pKa = 10.5 RR283 pKa = 11.84 IVGLASSFPKK293 pKa = 10.45 SAGSFDD299 pKa = 4.33 SVVCVKK305 pKa = 10.11 KK306 pKa = 10.6 ACWMNVPLFDD316 pKa = 4.25 VDD318 pKa = 3.85 VGKK321 pKa = 10.62 KK322 pKa = 10.5 GYY324 pKa = 8.14 GLRR327 pKa = 11.84 MIGDD331 pKa = 3.73 SAKK334 pKa = 10.7 LYY336 pKa = 9.41 IVKK339 pKa = 10.32 RR340 pKa = 11.84 GLSVMVLPAGVV351 pKa = 3.07
Molecular weight: 39.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.999
IPC2_protein 5.003
IPC_protein 4.914
Toseland 4.774
ProMoST 5.003
Dawson 4.838
Bjellqvist 4.991
Wikipedia 4.685
Rodwell 4.762
Grimsley 4.685
Solomon 4.838
Lehninger 4.8
Nozaki 4.94
DTASelect 5.067
Thurlkill 4.762
EMBOSS 4.698
Sillero 5.016
Patrickios 4.291
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 5.025
Protein with the highest isoelectric point:
>tr|H9ZXQ3|H9ZXQ3_9REOV Outer capsid protein VP2 OS=Tilligerry virus OX=1170505 PE=3 SV=1
MM1 pKa = 6.93 VATVRR6 pKa = 11.84 GVDD9 pKa = 2.95 IVDD12 pKa = 3.54 RR13 pKa = 11.84 VLKK16 pKa = 10.44 RR17 pKa = 11.84 FYY19 pKa = 9.35 PTAEE23 pKa = 3.65 FDD25 pKa = 3.55 YY26 pKa = 11.3 TRR28 pKa = 11.84 GFWHH32 pKa = 7.99 LYY34 pKa = 9.33 RR35 pKa = 11.84 FSSNIRR41 pKa = 11.84 IIRR44 pKa = 11.84 RR45 pKa = 11.84 KK46 pKa = 10.05 KK47 pKa = 6.99 GTKK50 pKa = 8.36 YY51 pKa = 10.51 RR52 pKa = 11.84 RR53 pKa = 11.84 DD54 pKa = 3.66 DD55 pKa = 3.89 DD56 pKa = 4.22 VLTNLVDD63 pKa = 3.69 RR64 pKa = 11.84 RR65 pKa = 11.84 SEE67 pKa = 3.87 TLYY70 pKa = 10.93 GIRR73 pKa = 11.84 VLNEE77 pKa = 3.78 ASWEE81 pKa = 4.07 EE82 pKa = 3.88 LTEE85 pKa = 4.33 EE86 pKa = 4.09 YY87 pKa = 10.61 SRR89 pKa = 11.84 RR90 pKa = 11.84 SKK92 pKa = 11.27 GLGIFEE98 pKa = 4.41 NSVLDD103 pKa = 4.1 HH104 pKa = 7.05 EE105 pKa = 4.72 EE106 pKa = 4.16 LDD108 pKa = 3.81 PEE110 pKa = 4.45 EE111 pKa = 4.23 EE112 pKa = 3.81 FLRR115 pKa = 11.84 NYY117 pKa = 10.33 RR118 pKa = 11.84 VIEE121 pKa = 4.68 DD122 pKa = 4.12 GAGHH126 pKa = 7.84 LSTFIEE132 pKa = 4.24 TRR134 pKa = 11.84 AKK136 pKa = 10.97 VEE138 pKa = 3.85 MQVYY142 pKa = 10.52 GDD144 pKa = 4.54 LPMKK148 pKa = 9.8 IWFIFVEE155 pKa = 4.38 QLCKK159 pKa = 10.37 HH160 pKa = 5.88 VGFEE164 pKa = 4.52 TEE166 pKa = 4.16 GLTVMKK172 pKa = 10.55 SFLFKK177 pKa = 10.83 YY178 pKa = 8.73 GAPFHH183 pKa = 6.39 QGLRR187 pKa = 11.84 DD188 pKa = 3.58 LSKK191 pKa = 11.28 LDD193 pKa = 3.79 GFKK196 pKa = 10.1 MSYY199 pKa = 7.62 STPMLFEE206 pKa = 4.12 MSCMEE211 pKa = 4.59 SLLEE215 pKa = 3.84 INIFKK220 pKa = 10.78 RR221 pKa = 11.84 MQEE224 pKa = 3.92 EE225 pKa = 4.6 RR226 pKa = 11.84 IHH228 pKa = 6.45 CLQFGDD234 pKa = 5.16 RR235 pKa = 11.84 EE236 pKa = 4.27 INPIALLRR244 pKa = 11.84 EE245 pKa = 4.73 FYY247 pKa = 10.52 LICLPHH253 pKa = 7.06 PKK255 pKa = 9.92 RR256 pKa = 11.84 INNILRR262 pKa = 11.84 SPYY265 pKa = 9.91 SWFVKK270 pKa = 8.79 TWGVAANPIVVLRR283 pKa = 11.84 SSGGDD288 pKa = 3.27 DD289 pKa = 3.61 RR290 pKa = 11.84 NSKK293 pKa = 10.34 DD294 pKa = 2.69 IYY296 pKa = 10.05 YY297 pKa = 10.46 EE298 pKa = 3.7 KK299 pKa = 10.53 FHH301 pKa = 7.29 YY302 pKa = 10.54 VEE304 pKa = 4.02 NTYY307 pKa = 10.7 VPIFNARR314 pKa = 11.84 FYY316 pKa = 11.04 RR317 pKa = 11.84 KK318 pKa = 9.79 SLEE321 pKa = 4.1 DD322 pKa = 3.34 NMNKK326 pKa = 9.43 VNEE329 pKa = 4.73 AIEE332 pKa = 4.13 YY333 pKa = 9.48 SQGLGQHH340 pKa = 5.94 EE341 pKa = 4.7 EE342 pKa = 4.1 GLPIFTSMLEE352 pKa = 4.19 EE353 pKa = 4.49 VYY355 pKa = 9.03 KK356 pKa = 10.85 TPFVPNKK363 pKa = 9.21 PSNLILASFLLSIQTITGYY382 pKa = 10.39 GRR384 pKa = 11.84 AWVKK388 pKa = 10.81 NVGTDD393 pKa = 3.7 FGKK396 pKa = 10.5 QMKK399 pKa = 9.28 PSKK402 pKa = 10.76 DD403 pKa = 3.33 NFIEE407 pKa = 4.14 RR408 pKa = 11.84 VADD411 pKa = 3.49 YY412 pKa = 8.13 TANNFIAAYY421 pKa = 9.4 EE422 pKa = 4.21 EE423 pKa = 4.3 ARR425 pKa = 11.84 QHH427 pKa = 6.46 HH428 pKa = 6.7 EE429 pKa = 4.76 DD430 pKa = 3.13 IVFPEE435 pKa = 4.19 DD436 pKa = 4.58 LYY438 pKa = 11.27 TSMLRR443 pKa = 11.84 LARR446 pKa = 11.84 NTSSGFSTSVKK457 pKa = 9.31 VRR459 pKa = 11.84 KK460 pKa = 9.9 AFGPNVKK467 pKa = 10.06 RR468 pKa = 11.84 KK469 pKa = 9.7 PEE471 pKa = 4.82 LIDD474 pKa = 3.04 VTSRR478 pKa = 11.84 IKK480 pKa = 11.02 ALVIFTKK487 pKa = 10.2 GHH489 pKa = 4.59 TVFTPEE495 pKa = 4.34 EE496 pKa = 4.08 LNKK499 pKa = 10.72 KK500 pKa = 10.14 YY501 pKa = 9.65 DD502 pKa = 3.26 TATNYY507 pKa = 6.54 QTKK510 pKa = 10.06 GSRR513 pKa = 11.84 EE514 pKa = 3.95 VPIKK518 pKa = 9.73 ATRR521 pKa = 11.84 TIYY524 pKa = 10.57 SINLSVLVPQLVVTLPLNEE543 pKa = 3.69 YY544 pKa = 8.82 FARR547 pKa = 11.84 VGGSTSPNCKK557 pKa = 9.88 RR558 pKa = 11.84 MGGKK562 pKa = 9.63 IIVGDD567 pKa = 4.15 LEE569 pKa = 4.43 ATGSRR574 pKa = 11.84 VMDD577 pKa = 4.22 AADD580 pKa = 3.62 TFRR583 pKa = 11.84 NSADD587 pKa = 3.23 PSTFTLAIDD596 pKa = 3.44 YY597 pKa = 10.86 SEE599 pKa = 5.11 YY600 pKa = 9.9 DD601 pKa = 3.35 THH603 pKa = 5.86 LTRR606 pKa = 11.84 YY607 pKa = 8.59 NFRR610 pKa = 11.84 NGMMRR615 pKa = 11.84 GLRR618 pKa = 11.84 AAMLKK623 pKa = 10.39 YY624 pKa = 10.09 RR625 pKa = 11.84 DD626 pKa = 4.03 LRR628 pKa = 11.84 YY629 pKa = 10.19 EE630 pKa = 4.54 GYY632 pKa = 10.69 SLDD635 pKa = 3.89 DD636 pKa = 5.44 LIDD639 pKa = 3.9 FGYY642 pKa = 11.11 GDD644 pKa = 4.23 GRR646 pKa = 11.84 VAMTLWNGKK655 pKa = 9.0 RR656 pKa = 11.84 RR657 pKa = 11.84 VFKK660 pKa = 11.11 VPAHH664 pKa = 6.39 LYY666 pKa = 10.2 IMLDD670 pKa = 3.35 EE671 pKa = 4.31 EE672 pKa = 4.61 EE673 pKa = 4.5 RR674 pKa = 11.84 RR675 pKa = 11.84 QGDD678 pKa = 3.72 FRR680 pKa = 11.84 PPPGVRR686 pKa = 11.84 PVTSMNVAEE695 pKa = 4.34 KK696 pKa = 10.33 LVRR699 pKa = 11.84 GKK701 pKa = 8.43 TVSDD705 pKa = 3.81 DD706 pKa = 4.26 FILISPTDD714 pKa = 3.79 GSDD717 pKa = 3.02 LALIDD722 pKa = 3.54 THH724 pKa = 8.54 LSGEE728 pKa = 4.13 NSTLIANSMHH738 pKa = 5.94 NMAIGTIIQEE748 pKa = 4.53 EE749 pKa = 4.45 VLKK752 pKa = 10.52 RR753 pKa = 11.84 YY754 pKa = 6.48 PTEE757 pKa = 3.61 ISFQSEE763 pKa = 4.62 QYY765 pKa = 11.15 VGDD768 pKa = 3.75 DD769 pKa = 3.37 TLLYY773 pKa = 9.14 TQLLTRR779 pKa = 11.84 DD780 pKa = 3.72 PRR782 pKa = 11.84 VVNDD786 pKa = 4.11 IIEE789 pKa = 4.51 TTFSSVEE796 pKa = 4.09 KK797 pKa = 10.23 CGHH800 pKa = 5.72 EE801 pKa = 3.94 ASASKK806 pKa = 9.93 TMVYY810 pKa = 9.78 PFSVEE815 pKa = 3.67 KK816 pKa = 9.41 TQTHH820 pKa = 6.66 AKK822 pKa = 8.94 QGIYY826 pKa = 10.29 VPQDD830 pKa = 2.45 RR831 pKa = 11.84 MMIISSEE838 pKa = 3.86 RR839 pKa = 11.84 RR840 pKa = 11.84 KK841 pKa = 10.73 DD842 pKa = 3.33 IEE844 pKa = 4.13 NVNGYY849 pKa = 7.96 MRR851 pKa = 11.84 SQVHH855 pKa = 4.65 TMVTKK860 pKa = 9.24 VSRR863 pKa = 11.84 GFSHH867 pKa = 7.84 DD868 pKa = 3.36 LAQWILMLKK877 pKa = 8.78 TVFVGAWKK885 pKa = 10.55 LKK887 pKa = 9.08 RR888 pKa = 11.84 TIKK891 pKa = 9.55 DD892 pKa = 2.98 THH894 pKa = 6.53 GYY896 pKa = 9.64 RR897 pKa = 11.84 DD898 pKa = 3.46 RR899 pKa = 11.84 KK900 pKa = 10.23 FDD902 pKa = 5.25 DD903 pKa = 3.7 DD904 pKa = 4.18 TEE906 pKa = 5.86 DD907 pKa = 3.57 GFTLVTLKK915 pKa = 10.99 NPLALHH921 pKa = 7.06 IPQNWGGYY929 pKa = 7.26 GAHH932 pKa = 6.97 PAALNIVMTEE942 pKa = 3.81 EE943 pKa = 3.95 MFIDD947 pKa = 3.78 SMQISKK953 pKa = 11.16 LEE955 pKa = 4.03 TEE957 pKa = 4.16 MQLLVKK963 pKa = 10.22 IAGKK967 pKa = 10.18 LPPAWDD973 pKa = 3.28 EE974 pKa = 4.06 TQADD978 pKa = 3.64 KK979 pKa = 11.21 RR980 pKa = 11.84 QISAEE985 pKa = 3.91 TKK987 pKa = 8.58 MSFFSKK993 pKa = 9.59 MARR996 pKa = 11.84 PAVRR1000 pKa = 11.84 ATLMVPEE1007 pKa = 4.45 IMDD1010 pKa = 3.63 VVEE1013 pKa = 4.42 TLPLGDD1019 pKa = 4.97 FGPGRR1024 pKa = 11.84 LSKK1027 pKa = 9.94 TMMHH1031 pKa = 6.35 SALLKK1036 pKa = 9.33 EE1037 pKa = 4.33 ANARR1041 pKa = 11.84 SLLVSSYY1048 pKa = 9.6 EE1049 pKa = 4.57 LEE1051 pKa = 4.15 YY1052 pKa = 10.8 QKK1054 pKa = 10.83 QLNGWKK1060 pKa = 8.75 EE1061 pKa = 3.88 QPMCFTLDD1069 pKa = 3.45 EE1070 pKa = 4.37 EE1071 pKa = 4.52 SGYY1074 pKa = 11.07 VSSKK1078 pKa = 7.27 YY1079 pKa = 11.12 AKK1081 pKa = 9.92 MFDD1084 pKa = 2.82 IHH1086 pKa = 7.89 FDD1088 pKa = 3.73 EE1089 pKa = 6.04 EE1090 pKa = 4.39 IMEE1093 pKa = 4.61 PAHH1096 pKa = 6.59 VFPDD1100 pKa = 3.77 QNLSPQFYY1108 pKa = 8.4 VQKK1111 pKa = 10.87 AIIGQRR1117 pKa = 11.84 KK1118 pKa = 5.02 STRR1121 pKa = 11.84 MRR1123 pKa = 11.84 MSYY1126 pKa = 10.02 IDD1128 pKa = 4.79 RR1129 pKa = 11.84 IDD1131 pKa = 4.37 AILRR1135 pKa = 11.84 KK1136 pKa = 9.59 DD1137 pKa = 3.37 VVMRR1141 pKa = 11.84 GFLTANTIVNVLEE1154 pKa = 4.21 KK1155 pKa = 10.99 VGVSHH1160 pKa = 6.69 TAVDD1164 pKa = 4.47 LVTLFTLMNIEE1175 pKa = 4.04 VKK1177 pKa = 10.55 VAEE1180 pKa = 4.48 EE1181 pKa = 3.58 LAEE1184 pKa = 4.37 YY1185 pKa = 8.54 ITSEE1189 pKa = 4.11 RR1190 pKa = 11.84 VRR1192 pKa = 11.84 FDD1194 pKa = 4.37 AIKK1197 pKa = 10.23 LLKK1200 pKa = 10.2 KK1201 pKa = 10.64 GIVGDD1206 pKa = 4.18 EE1207 pKa = 4.08 FSMSLDD1213 pKa = 3.2 VATQDD1218 pKa = 5.24 MIDD1221 pKa = 3.25 QLIRR1225 pKa = 11.84 YY1226 pKa = 6.75 PHH1228 pKa = 6.93 EE1229 pKa = 3.98 LTKK1232 pKa = 10.77 TEE1234 pKa = 4.13 LDD1236 pKa = 3.46 AVSLYY1241 pKa = 10.56 VSQLIMLRR1249 pKa = 11.84 AALGMKK1255 pKa = 9.56 KK1256 pKa = 9.99 RR1257 pKa = 11.84 RR1258 pKa = 11.84 IRR1260 pKa = 11.84 VSISPDD1266 pKa = 2.64 EE1267 pKa = 4.32 RR1268 pKa = 11.84 EE1269 pKa = 3.68 RR1270 pKa = 11.84 FRR1272 pKa = 11.84 AKK1274 pKa = 8.07 VQRR1277 pKa = 11.84 YY1278 pKa = 6.42 KK1279 pKa = 10.08 THH1281 pKa = 5.41 TPKK1284 pKa = 10.92 LKK1286 pKa = 10.52 LIKK1289 pKa = 10.24 KK1290 pKa = 9.93 LIDD1293 pKa = 3.21 INRR1296 pKa = 11.84 LSVRR1300 pKa = 11.84 ALEE1303 pKa = 4.21 NQFVV1307 pKa = 3.34
Molecular weight: 150.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.251
IPC2_protein 7.688
IPC_protein 7.585
Toseland 7.732
ProMoST 8.112
Dawson 8.39
Bjellqvist 8.434
Wikipedia 8.404
Rodwell 8.448
Grimsley 7.878
Solomon 8.448
Lehninger 8.434
Nozaki 8.478
DTASelect 8.331
Thurlkill 8.404
EMBOSS 8.565
Sillero 8.609
Patrickios 4.533
IPC_peptide 8.448
IPC2_peptide 7.249
IPC2.peptide.svr19 7.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
6183
242
1307
618.3
70.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.728 ± 0.536
1.213 ± 0.352
6.065 ± 0.319
7.618 ± 0.461
3.898 ± 0.262
6.243 ± 0.492
2.394 ± 0.322
6.211 ± 0.343
5.79 ± 0.67
8.572 ± 0.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.801 ± 0.233
3.962 ± 0.273
3.962 ± 0.328
3.186 ± 0.356
6.955 ± 0.353
5.839 ± 0.358
5.386 ± 0.44
7.116 ± 0.342
1.472 ± 0.285
3.59 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here