Podoviridae sp. ctbj_2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; unclassified Zobellviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MKY7|A0A345MKY7_9CAUD HNH endonuclease OS=Podoviridae sp. ctbj_2 OX=2675442 PE=4 SV=1
MM1 pKa = 6.72MQAAYY6 pKa = 10.15CKK8 pKa = 10.96ANMDD12 pKa = 4.0LEE14 pKa = 4.53KK15 pKa = 10.57KK16 pKa = 9.78YY17 pKa = 11.22VPGEE21 pKa = 4.02DD22 pKa = 3.19FGFVCWMHH30 pKa = 7.39DD31 pKa = 3.4EE32 pKa = 4.18FTIEE36 pKa = 4.56CKK38 pKa = 10.43PEE40 pKa = 3.55IADD43 pKa = 3.59EE44 pKa = 4.32VKK46 pKa = 10.55RR47 pKa = 11.84IAEE50 pKa = 4.08EE51 pKa = 4.25SIVWAGEE58 pKa = 3.8FFKK61 pKa = 10.73ILCPHH66 pKa = 6.83IGDD69 pKa = 4.34GKK71 pKa = 10.68IGTDD75 pKa = 3.03WYY77 pKa = 10.79SIHH80 pKa = 7.08

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MKW8|A0A345MKW8_9CAUD Putative endonuclease OS=Podoviridae sp. ctbj_2 OX=2675442 PE=4 SV=1
MM1 pKa = 7.41AAVTTIVAVAGLALAAGGAAMSYY24 pKa = 9.07SQQRR28 pKa = 11.84RR29 pKa = 11.84ANAQQARR36 pKa = 11.84AEE38 pKa = 4.22EE39 pKa = 4.14QQKK42 pKa = 9.39QAQAEE47 pKa = 4.25EE48 pKa = 4.34SANRR52 pKa = 11.84ASQAAAEE59 pKa = 4.42RR60 pKa = 11.84RR61 pKa = 11.84TQVRR65 pKa = 11.84EE66 pKa = 3.73QRR68 pKa = 11.84IKK70 pKa = 10.6RR71 pKa = 11.84AQILQAAEE79 pKa = 3.95NTGTSGSSGEE89 pKa = 4.14GGALGSLSTQLSANLGTNEE108 pKa = 4.58AGFQRR113 pKa = 11.84GQRR116 pKa = 11.84ISMFNQRR123 pKa = 11.84AANYY127 pKa = 10.17LGDD130 pKa = 3.91AQVSQGRR137 pKa = 11.84GQMYY141 pKa = 10.08SQIGQLGSAMFSAAGGMKK159 pKa = 10.19SIMKK163 pKa = 8.94PQAIPAQSGSWIGTWGDD180 pKa = 2.92NFKK183 pKa = 10.87RR184 pKa = 11.84VGG186 pKa = 3.26

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

14988

80

1669

267.6

29.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.507 ± 0.482

1.121 ± 0.206

5.805 ± 0.296

6.612 ± 0.32

3.983 ± 0.2

7.379 ± 0.236

2.035 ± 0.221

5.304 ± 0.175

6.218 ± 0.364

7.76 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.976 ± 0.231

5.331 ± 0.217

3.883 ± 0.153

4.163 ± 0.257

5.651 ± 0.177

6.031 ± 0.25

5.671 ± 0.274

6.492 ± 0.242

1.468 ± 0.104

3.61 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski