Macaca fascicularis papillomavirus 2
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8QPP9|F8QPP9_9PAPI Minor capsid protein L2 OS=Macaca fascicularis papillomavirus 2 OX=915424 GN=L2 PE=3 SV=1
MM1 pKa = 7.32 LHH3 pKa = 6.87 LGLMQRR9 pKa = 11.84 DD10 pKa = 3.91 MVKK13 pKa = 10.64 LDD15 pKa = 3.66 FGRR18 pKa = 11.84 LSIIRR23 pKa = 11.84 TLSLLLSPVQRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 DD37 pKa = 3.62 RR38 pKa = 11.84 DD39 pKa = 3.69 HH40 pKa = 6.94 QPPPTPTPQKK50 pKa = 10.94 DD51 pKa = 3.31 PNTPLKK57 pKa = 9.19 WYY59 pKa = 10.3 QSIRR63 pKa = 11.84 PPPLPPQPPLPPLNSEE79 pKa = 4.21 PEE81 pKa = 4.33 GTGEE85 pKa = 3.93 KK86 pKa = 10.43 PPVQEE91 pKa = 4.98 EE92 pKa = 4.38 EE93 pKa = 4.48 NEE95 pKa = 3.93 DD96 pKa = 3.48 TRR98 pKa = 11.84 PSKK101 pKa = 9.47 KK102 pKa = 8.73 TKK104 pKa = 10.28 EE105 pKa = 4.34 NGDD108 pKa = 3.55 HH109 pKa = 6.43 TSGDD113 pKa = 3.9 GEE115 pKa = 4.54 KK116 pKa = 10.64 EE117 pKa = 4.18 GGDD120 pKa = 3.95 PDD122 pKa = 4.94 PGPVPHH128 pKa = 7.82 PDD130 pKa = 4.02 PDD132 pKa = 4.71 PDD134 pKa = 4.76 PDD136 pKa = 4.38 PEE138 pKa = 4.78 PEE140 pKa = 4.65 PEE142 pKa = 4.87 PDD144 pKa = 4.7 PNPQPQPIPAPDD156 pKa = 4.7 PDD158 pKa = 4.69 LDD160 pKa = 4.46 PGQVASFLEE169 pKa = 5.05 GVASYY174 pKa = 9.04 LHH176 pKa = 6.4 RR177 pKa = 11.84 WEE179 pKa = 4.56 VGYY182 pKa = 9.67 QQLVKK187 pKa = 10.47 DD188 pKa = 4.08 IKK190 pKa = 10.91 EE191 pKa = 4.24 DD192 pKa = 4.16 LDD194 pKa = 4.21 NYY196 pKa = 8.24 WLKK199 pKa = 11.0 LQTPQQ204 pKa = 4.22
Molecular weight: 22.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.658
IPC2_protein 4.546
IPC_protein 4.495
Toseland 4.329
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.355
Rodwell 4.329
Grimsley 4.24
Solomon 4.457
Lehninger 4.418
Nozaki 4.571
DTASelect 4.774
Thurlkill 4.342
EMBOSS 4.368
Sillero 4.609
Patrickios 4.151
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.545
Protein with the highest isoelectric point:
>tr|F8QPP8|F8QPP8_9PAPI E4 OS=Macaca fascicularis papillomavirus 2 OX=915424 GN=E4 PE=4 SV=1
MM1 pKa = 7.02 EE2 pKa = 4.44 TLSEE6 pKa = 4.19 RR7 pKa = 11.84 FHH9 pKa = 7.09 ALQEE13 pKa = 4.13 KK14 pKa = 10.35 LMEE17 pKa = 4.88 IYY19 pKa = 10.51 EE20 pKa = 4.34 SGKK23 pKa = 8.49 NTIAAQIEE31 pKa = 4.3 HH32 pKa = 6.22 WQLLRR37 pKa = 11.84 QEE39 pKa = 4.16 QVLLHH44 pKa = 6.15 FARR47 pKa = 11.84 KK48 pKa = 9.64 RR49 pKa = 11.84 GVTRR53 pKa = 11.84 LGYY56 pKa = 10.31 QPVPNLAITEE66 pKa = 4.17 QKK68 pKa = 10.94 AKK70 pKa = 10.68 DD71 pKa = 3.99 AIGMGLLLQSLEE83 pKa = 3.82 KK84 pKa = 10.53 SPYY87 pKa = 10.15 GGEE90 pKa = 3.9 SWSLVDD96 pKa = 4.03 TSLEE100 pKa = 4.27 TFRR103 pKa = 11.84 SPPQQCFKK111 pKa = 11.06 KK112 pKa = 10.31 GGQEE116 pKa = 3.79 VEE118 pKa = 4.09 VRR120 pKa = 11.84 FDD122 pKa = 3.81 GDD124 pKa = 3.54 PEE126 pKa = 5.48 NIMLYY131 pKa = 9.73 TSWQHH136 pKa = 7.26 IYY138 pKa = 10.37 FQDD141 pKa = 4.85 SHH143 pKa = 8.03 DD144 pKa = 3.76 SWQKK148 pKa = 9.55 VQGQVDD154 pKa = 4.2 YY155 pKa = 11.62 DD156 pKa = 3.56 GLFYY160 pKa = 10.79 FDD162 pKa = 4.98 GPVKK166 pKa = 10.49 VYY168 pKa = 10.68 YY169 pKa = 9.34 VTFGTDD175 pKa = 2.57 AKK177 pKa = 10.61 RR178 pKa = 11.84 YY179 pKa = 8.62 GKK181 pKa = 8.54 TGLWEE186 pKa = 3.8 VVYY189 pKa = 11.08 NKK191 pKa = 10.4 DD192 pKa = 3.68 IIFAPVTSSTPEE204 pKa = 4.17 GQGSPAPADD213 pKa = 3.62 SNTAEE218 pKa = 4.78 GSQHH222 pKa = 4.79 PTEE225 pKa = 4.42 VVSVDD230 pKa = 3.3 SATTSAPAATSATAKK245 pKa = 9.77 QRR247 pKa = 11.84 ARR249 pKa = 11.84 RR250 pKa = 11.84 YY251 pKa = 8.85 GRR253 pKa = 11.84 KK254 pKa = 9.39 ASSPGGGEE262 pKa = 3.61 RR263 pKa = 11.84 GYY265 pKa = 11.34 SPLKK269 pKa = 8.94 EE270 pKa = 4.21 NQRR273 pKa = 11.84 EE274 pKa = 4.11 RR275 pKa = 11.84 RR276 pKa = 11.84 PHH278 pKa = 4.85 KK279 pKa = 9.9 RR280 pKa = 11.84 GRR282 pKa = 11.84 GEE284 pKa = 3.5 RR285 pKa = 11.84 GGRR288 pKa = 11.84 SRR290 pKa = 11.84 SRR292 pKa = 11.84 SRR294 pKa = 11.84 ATSRR298 pKa = 11.84 SRR300 pKa = 11.84 SRR302 pKa = 11.84 SRR304 pKa = 11.84 SRR306 pKa = 11.84 TRR308 pKa = 11.84 TRR310 pKa = 11.84 ARR312 pKa = 11.84 PQSATTTHH320 pKa = 6.9 PCTRR324 pKa = 11.84 SRR326 pKa = 11.84 SRR328 pKa = 11.84 SRR330 pKa = 11.84 PGCVLSGGGGVLPSQVGSRR349 pKa = 11.84 LSTVGQGHH357 pKa = 5.81 QGRR360 pKa = 11.84 LGQLLAEE367 pKa = 4.56 ATDD370 pKa = 3.88 PPVILLRR377 pKa = 11.84 GDD379 pKa = 3.61 PNAVKK384 pKa = 10.59 CFRR387 pKa = 11.84 FRR389 pKa = 11.84 AKK391 pKa = 10.45 DD392 pKa = 3.36 KK393 pKa = 11.24 YY394 pKa = 9.79 RR395 pKa = 11.84 GLYY398 pKa = 9.2 TRR400 pKa = 11.84 MSTTWSWVGADD411 pKa = 3.24 NNDD414 pKa = 3.91 RR415 pKa = 11.84 IGRR418 pKa = 11.84 ARR420 pKa = 11.84 MLVAFSSYY428 pKa = 6.95 EE429 pKa = 3.81 QRR431 pKa = 11.84 HH432 pKa = 5.89 LFLKK436 pKa = 9.87 HH437 pKa = 6.01 LKK439 pKa = 9.61 VPKK442 pKa = 10.66 GMDD445 pKa = 2.83 WSLGQFDD452 pKa = 4.27 SLL454 pKa = 4.55
Molecular weight: 50.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.414
IPC_protein 9.706
Toseland 10.116
ProMoST 9.838
Dawson 10.321
Bjellqvist 10.014
Wikipedia 10.511
Rodwell 10.599
Grimsley 10.394
Solomon 10.35
Lehninger 10.321
Nozaki 10.116
DTASelect 10.014
Thurlkill 10.175
EMBOSS 10.526
Sillero 10.233
Patrickios 10.087
IPC_peptide 10.35
IPC2_peptide 8.785
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2558
108
614
365.4
40.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.059 ± 0.691
1.798 ± 0.587
6.568 ± 0.672
6.177 ± 0.953
4.027 ± 0.43
7.232 ± 0.759
1.916 ± 0.226
4.183 ± 0.419
3.987 ± 0.568
8.913 ± 0.512
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.446 ± 0.297
3.675 ± 0.792
6.88 ± 1.344
5.238 ± 0.274
7.271 ± 0.645
7.545 ± 0.799
6.138 ± 0.577
6.333 ± 0.554
1.407 ± 0.35
3.206 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here