Pseudoscardovia suis
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1732 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261EXE0|A0A261EXE0_9BIFI Phosphomethylpyrimidine synthase OS=Pseudoscardovia suis OX=987063 GN=thiC PE=3 SV=1
MM1 pKa = 7.66 ALNTTLLKK9 pKa = 10.6 RR10 pKa = 11.84 LAALGCACALSLGASACGSKK30 pKa = 9.96 QASTTSSPTASTSFTHH46 pKa = 6.4 MDD48 pKa = 4.85 GITATGNLGEE58 pKa = 4.21 QPTVSFDD65 pKa = 3.37 SLPYY69 pKa = 8.5 TVHH72 pKa = 6.1 NNSYY76 pKa = 11.09 AILQTGDD83 pKa = 3.24 GDD85 pKa = 4.29 TVQDD89 pKa = 3.72 GQRR92 pKa = 11.84 ICLQNMFVNTDD103 pKa = 3.61 DD104 pKa = 4.74 GSVLSSSWGSDD115 pKa = 3.02 PVDD118 pKa = 3.93 CATITQSQTPSDD130 pKa = 4.32 YY131 pKa = 11.24 YY132 pKa = 11.54 GLLVGQKK139 pKa = 8.27 VHH141 pKa = 4.7 TTIAIGYY148 pKa = 8.46 NLDD151 pKa = 3.71 EE152 pKa = 4.68 TSSSSSDD159 pKa = 3.22 SAANSAATATNSAQRR174 pKa = 11.84 LASASASSTGLSSLAALNDD193 pKa = 3.59 FTATVVDD200 pKa = 5.17 DD201 pKa = 4.63 EE202 pKa = 4.91 SQSSSASDD210 pKa = 3.35 SASASSSDD218 pKa = 3.67 SASSDD223 pKa = 3.4 STSSDD228 pKa = 3.31 STSSSSSDD236 pKa = 3.19 STSSTNVNSHH246 pKa = 5.59 YY247 pKa = 10.8 LWVFTIMSATQPATRR262 pKa = 11.84 AQGTEE267 pKa = 4.08 VTDD270 pKa = 4.23 LPSGLPTVTRR280 pKa = 11.84 DD281 pKa = 3.15 ASTGQPSIDD290 pKa = 3.03 VSTYY294 pKa = 10.4 SGSTTSLVSQTLIQGTGATVQQGDD318 pKa = 4.39 TLDD321 pKa = 3.42 VMYY324 pKa = 10.71 SGWLTDD330 pKa = 3.37 GTQFDD335 pKa = 5.21 SNWSNGTSTQLTMSEE350 pKa = 4.1 DD351 pKa = 3.73 SVIKK355 pKa = 10.7 GFMQGLVGQKK365 pKa = 9.93 VGSQVLLIIPPDD377 pKa = 3.54 LGYY380 pKa = 11.06 GSSAQGSIPANSTLIFVVDD399 pKa = 3.42 ILYY402 pKa = 10.81 SSSSKK407 pKa = 11.08
Molecular weight: 41.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.77
IPC_protein 3.821
Toseland 3.567
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.821
Rodwell 3.63
Grimsley 3.478
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.279
Thurlkill 3.643
EMBOSS 3.821
Sillero 3.948
Patrickios 0.731
IPC_peptide 3.821
IPC2_peptide 3.91
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A261F4G4|A0A261F4G4_9BIFI SNARE associated Golgi protein OS=Pseudoscardovia suis OX=987063 GN=PSSU_0132 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.83 MKK15 pKa = 9.27 HH16 pKa = 5.56 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ATINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 6.41 TLSAA44 pKa = 4.15
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1732
0
1732
640469
29
3158
369.8
40.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.577 ± 0.085
1.06 ± 0.023
6.869 ± 0.064
5.199 ± 0.052
3.12 ± 0.034
7.976 ± 0.052
2.311 ± 0.031
4.89 ± 0.04
3.558 ± 0.054
8.19 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.026
3.185 ± 0.036
4.775 ± 0.045
3.803 ± 0.043
6.028 ± 0.071
6.876 ± 0.081
6.475 ± 0.072
7.66 ± 0.055
1.346 ± 0.02
2.592 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here