Lishizhenia tianjinensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Lishizhenia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6Y9N8|A0A1I6Y9N8_9FLAO Uncharacterized protein OS=Lishizhenia tianjinensis OX=477690 GN=SAMN05216474_0747 PE=4 SV=1
MM1 pKa = 7.56LFASLFVVPFVMGQTEE17 pKa = 4.38VFNEE21 pKa = 4.04DD22 pKa = 3.23FQQGVPSTWTIVDD35 pKa = 3.57NDD37 pKa = 3.92GLTVDD42 pKa = 3.77SSVVEE47 pKa = 5.51FDD49 pKa = 4.26AAWITYY55 pKa = 9.21TEE57 pKa = 4.47DD58 pKa = 2.74SLYY61 pKa = 10.9ADD63 pKa = 4.19TVAASTSYY71 pKa = 10.94FDD73 pKa = 5.46PIDD76 pKa = 3.73RR77 pKa = 11.84ADD79 pKa = 3.6RR80 pKa = 11.84WLITPPINLGAYY92 pKa = 10.01GNILNWEE99 pKa = 4.26AMSADD104 pKa = 3.55PSFPDD109 pKa = 4.06DD110 pKa = 3.84YY111 pKa = 11.38KK112 pKa = 11.75VLISTTDD119 pKa = 3.32NNISSFTDD127 pKa = 3.11TLILVNNEE135 pKa = 3.18VPYY138 pKa = 9.65WLEE141 pKa = 4.03RR142 pKa = 11.84EE143 pKa = 4.45VNLSDD148 pKa = 4.93NGYY151 pKa = 10.07NDD153 pKa = 3.22QAVYY157 pKa = 10.27LAFVNTTFNGFKK169 pKa = 10.81LFIDD173 pKa = 3.96DD174 pKa = 3.65VKK176 pKa = 11.4VRR178 pKa = 11.84IDD180 pKa = 3.91DD181 pKa = 4.09PLSIEE186 pKa = 4.0NEE188 pKa = 4.0KK189 pKa = 10.8LVALTVFPNPTSDD202 pKa = 2.82MMYY205 pKa = 9.51ISSSKK210 pKa = 10.48AVNFSVINLEE220 pKa = 4.02GQVVIEE226 pKa = 4.05PQIGTEE232 pKa = 4.02CSMAHH237 pKa = 6.85LPQGVYY243 pKa = 10.36FLQYY247 pKa = 9.86EE248 pKa = 4.57VNGSYY253 pKa = 10.46GVEE256 pKa = 3.9RR257 pKa = 11.84IVKK260 pKa = 8.96QQ261 pKa = 3.3

Molecular weight: 29.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6XC80|A0A1I6XC80_9FLAO Por secretion system C-terminal sorting domain-containing protein OS=Lishizhenia tianjinensis OX=477690 GN=SAMN05216474_0079 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSQRR11 pKa = 11.84KK12 pKa = 8.72RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.56HH17 pKa = 3.99GFRR20 pKa = 11.84SRR22 pKa = 11.84MATKK26 pKa = 10.46NGRR29 pKa = 11.84KK30 pKa = 9.13VLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.17GRR40 pKa = 11.84KK41 pKa = 9.01SLTVSDD47 pKa = 3.79NKK49 pKa = 10.3LAKK52 pKa = 10.14RR53 pKa = 3.77

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3112

0

3112

1077000

29

3971

346.1

39.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.59 ± 0.056

0.947 ± 0.021

5.541 ± 0.031

6.832 ± 0.058

5.185 ± 0.04

6.542 ± 0.047

1.9 ± 0.025

7.112 ± 0.037

6.935 ± 0.07

9.543 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.025

5.985 ± 0.053

3.378 ± 0.027

3.67 ± 0.024

3.379 ± 0.034

6.415 ± 0.038

5.891 ± 0.086

6.445 ± 0.042

1.072 ± 0.014

4.352 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski