Phyllobacterium leguminum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Phyllobacterium

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3580 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318TJ87|A0A318TJ87_9RHIZ 16S rRNA m(2)G 1207 methyltransferase OS=Phyllobacterium leguminum OX=314237 GN=C7477_10465 PE=4 SV=1
MM1 pKa = 7.71VDD3 pKa = 3.75PLDD6 pKa = 4.77PNLQPGRR13 pKa = 11.84TPPIDD18 pKa = 4.34DD19 pKa = 4.47PLVDD23 pKa = 4.42RR24 pKa = 11.84PRR26 pKa = 11.84PMDD29 pKa = 3.61SGAPGGGIASSTYY42 pKa = 10.02LWAAVLLVIILGALYY57 pKa = 10.03YY58 pKa = 10.31VYY60 pKa = 10.87SSGDD64 pKa = 3.51MASAPPPPQPATPTAPAPGG83 pKa = 3.53

Molecular weight: 8.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318TL10|A0A318TL10_9RHIZ Na+/H+-dicarboxylate symporter OS=Phyllobacterium leguminum OX=314237 GN=C7477_102237 PE=4 SV=1
MM1 pKa = 7.23HH2 pKa = 7.65RR3 pKa = 11.84KK4 pKa = 9.0FLTTLFASTFVLGLGGASLAAGRR27 pKa = 11.84TAHH30 pKa = 6.45EE31 pKa = 4.22AAPRR35 pKa = 11.84PSVICGTSAASCGFRR50 pKa = 11.84HH51 pKa = 5.81TKK53 pKa = 9.93ASKK56 pKa = 10.26APRR59 pKa = 11.84ATRR62 pKa = 11.84STPTAAYY69 pKa = 9.77APAIGTGAVASVNPATGTRR88 pKa = 11.84MPGPDD93 pKa = 3.5IGSGATPAPTSIVRR107 pKa = 11.84IPLGSRR113 pKa = 11.84SNGRR117 pKa = 11.84VRR119 pKa = 11.84IGSGATVDD127 pKa = 3.7RR128 pKa = 11.84APIGKK133 pKa = 9.55GVTVGAAPIGSGATVSRR150 pKa = 11.84NPASGRR156 pKa = 11.84AASLARR162 pKa = 11.84HH163 pKa = 5.04SAIGKK168 pKa = 8.65GAAVGPAPIGSGATVSRR185 pKa = 11.84NPAGGRR191 pKa = 11.84AASLARR197 pKa = 11.84HH198 pKa = 5.04SAIGKK203 pKa = 8.65GAAVGPAPIGSGATVSRR220 pKa = 11.84NPAGGRR226 pKa = 11.84AASLARR232 pKa = 11.84HH233 pKa = 5.97SATASLARR241 pKa = 11.84HH242 pKa = 5.65SANPASGKK250 pKa = 9.83AASLARR256 pKa = 11.84HH257 pKa = 5.98SATASLAQHH266 pKa = 6.36SPNPAGGKK274 pKa = 9.17AASLAHH280 pKa = 6.3HH281 pKa = 6.67SATASLAQHH290 pKa = 6.37SATPASRR297 pKa = 11.84NTASLARR304 pKa = 11.84HH305 pKa = 5.55SATGKK310 pKa = 9.81GVARR314 pKa = 11.84HH315 pKa = 5.43SASTGHH321 pKa = 6.62HH322 pKa = 4.74NHH324 pKa = 6.72GGHH327 pKa = 6.71RR328 pKa = 11.84GHH330 pKa = 7.36RR331 pKa = 11.84GPSTRR336 pKa = 4.17

Molecular weight: 32.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3580

0

3580

1086818

26

2950

303.6

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.833 ± 0.053

0.731 ± 0.011

5.613 ± 0.03

5.847 ± 0.05

3.899 ± 0.029

8.509 ± 0.043

1.983 ± 0.023

5.843 ± 0.033

3.964 ± 0.04

9.789 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.021

2.972 ± 0.032

5.038 ± 0.034

3.083 ± 0.021

6.683 ± 0.049

5.547 ± 0.028

5.378 ± 0.047

7.07 ± 0.032

1.276 ± 0.018

2.388 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski