Helcococcus ovis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Helcococcus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1566 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R9C2Y4|A0A4R9C2Y4_9FIRM RNase H OS=Helcococcus ovis OX=72026 GN=EQF91_05725 PE=3 SV=1
MM1 pKa = 7.63TDD3 pKa = 2.95KK4 pKa = 11.17LDD6 pKa = 3.85YY7 pKa = 11.11EE8 pKa = 4.49NEE10 pKa = 4.11NLDD13 pKa = 5.07DD14 pKa = 3.68EE15 pKa = 5.22HH16 pKa = 9.0LDD18 pKa = 3.53EE19 pKa = 5.75EE20 pKa = 4.74EE21 pKa = 4.38DD22 pKa = 3.82FKK24 pKa = 11.68EE25 pKa = 4.96VILTLDD31 pKa = 4.43DD32 pKa = 3.99DD33 pKa = 4.7TEE35 pKa = 4.4LKK37 pKa = 10.82CLVIAQYY44 pKa = 10.95DD45 pKa = 4.08VNKK48 pKa = 10.02QDD50 pKa = 4.58YY51 pKa = 9.98IALLPIEE58 pKa = 5.01DD59 pKa = 4.96DD60 pKa = 3.86EE61 pKa = 5.66PGEE64 pKa = 3.96ILLYY68 pKa = 10.03RR69 pKa = 11.84ATYY72 pKa = 10.76GEE74 pKa = 5.24DD75 pKa = 3.1EE76 pKa = 4.49TFDD79 pKa = 3.3VSLIEE84 pKa = 5.01DD85 pKa = 3.54EE86 pKa = 5.84EE87 pKa = 4.46EE88 pKa = 3.96FQVAADD94 pKa = 4.1AYY96 pKa = 8.76YY97 pKa = 10.88NYY99 pKa = 9.86IEE101 pKa = 4.53EE102 pKa = 4.35EE103 pKa = 4.34EE104 pKa = 4.46IDD106 pKa = 3.77VDD108 pKa = 4.33SIDD111 pKa = 3.82YY112 pKa = 10.52HH113 pKa = 5.6EE114 pKa = 4.97HH115 pKa = 5.27HH116 pKa = 6.86HH117 pKa = 5.58HH118 pKa = 6.55HH119 pKa = 6.74KK120 pKa = 10.59EE121 pKa = 3.81EE122 pKa = 4.48HH123 pKa = 5.59EE124 pKa = 4.7CKK126 pKa = 10.13CGHH129 pKa = 5.88HH130 pKa = 5.96HH131 pKa = 7.13HH132 pKa = 6.6EE133 pKa = 4.75EE134 pKa = 3.9EE135 pKa = 4.35HH136 pKa = 6.59KK137 pKa = 10.95CNCEE141 pKa = 4.07HH142 pKa = 6.81KK143 pKa = 10.41HH144 pKa = 4.92EE145 pKa = 4.28

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R9C566|A0A4R9C566_9FIRM Cation-translocating P-type ATPase OS=Helcococcus ovis OX=72026 GN=EQF91_00570 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.25QPNRR9 pKa = 11.84RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.73KK15 pKa = 8.7HH16 pKa = 4.98GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.51GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1566

0

1566

500755

35

2917

319.8

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.932 ± 0.07

0.589 ± 0.017

5.707 ± 0.051

7.341 ± 0.066

4.973 ± 0.045

5.863 ± 0.058

1.312 ± 0.021

10.278 ± 0.072

9.922 ± 0.084

9.245 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.03

7.001 ± 0.069

2.513 ± 0.03

2.394 ± 0.026

3.225 ± 0.042

6.436 ± 0.038

4.6 ± 0.041

6.066 ± 0.052

0.671 ± 0.021

4.502 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski