Adlercreutzia caecicola
Average proteome isoelectric point is 5.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1821 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4T9TEF9|A0A4T9TEF9_9ACTN VanZ domain-containing protein OS=Adlercreutzia caecicola OX=747645 GN=E5982_02910 PE=4 SV=1
MM1 pKa = 7.15 TRR3 pKa = 11.84 QDD5 pKa = 3.77 EE6 pKa = 4.17 FDD8 pKa = 3.2 AAARR12 pKa = 11.84 ALRR15 pKa = 11.84 ADD17 pKa = 4.33 AEE19 pKa = 4.3 QHH21 pKa = 5.97 PVPASLQPEE30 pKa = 4.62 AIEE33 pKa = 4.77 AMLLAHH39 pKa = 7.47 DD40 pKa = 4.42 AQKK43 pKa = 11.23 SDD45 pKa = 4.2 GEE47 pKa = 4.58 TADD50 pKa = 3.42 SGQPASAAPAPVEE63 pKa = 4.27 TGSAAAGTASPRR75 pKa = 11.84 RR76 pKa = 11.84 HH77 pKa = 5.01 VTWRR81 pKa = 11.84 WPIAAAACLALLCGVGVVGALAAQGKK107 pKa = 8.62 FSPAAIDD114 pKa = 4.16 APSSPLPAVSDD125 pKa = 4.22 PSAPASPEE133 pKa = 4.14 SGLSTAQGYY142 pKa = 8.72 AQVRR146 pKa = 11.84 DD147 pKa = 4.08 CLLASQQAMGDD158 pKa = 3.43 GGTMFMEE165 pKa = 5.3 DD166 pKa = 2.95 KK167 pKa = 9.88 TAGVARR173 pKa = 11.84 TEE175 pKa = 3.97 TATAFTAQNSTKK187 pKa = 10.43 DD188 pKa = 3.46 SSFTDD193 pKa = 3.3 TNEE196 pKa = 3.71 RR197 pKa = 11.84 TEE199 pKa = 4.81 GIGEE203 pKa = 4.28 ADD205 pKa = 3.53 VAKK208 pKa = 10.16 TDD210 pKa = 3.99 GRR212 pKa = 11.84 WLYY215 pKa = 11.12 ALQDD219 pKa = 3.28 SGTTVAIVDD228 pKa = 3.84 PAEE231 pKa = 4.05 GHH233 pKa = 5.17 MTPAGAIEE241 pKa = 4.31 APKK244 pKa = 9.8 GAQVSEE250 pKa = 5.06 FYY252 pKa = 11.33 VEE254 pKa = 4.82 DD255 pKa = 3.45 GRR257 pKa = 11.84 LYY259 pKa = 11.04 LLSTVFPEE267 pKa = 5.44 GVEE270 pKa = 4.28 QPDD273 pKa = 3.59 GTYY276 pKa = 9.82 TFPPEE281 pKa = 3.86 TTRR284 pKa = 11.84 LDD286 pKa = 3.87 VYY288 pKa = 11.33 DD289 pKa = 3.75 VTDD292 pKa = 3.91 PANPQATGLLTQTGTYY308 pKa = 7.85 NTVRR312 pKa = 11.84 FADD315 pKa = 4.54 GYY317 pKa = 11.24 VYY319 pKa = 10.8 LFSDD323 pKa = 2.84 QWTYY327 pKa = 10.57 DD328 pKa = 3.36 TSEE331 pKa = 4.1 GTDD334 pKa = 3.25 PSAYY338 pKa = 9.62 VPCVNGAAVEE348 pKa = 4.31 CGDD351 pKa = 4.37 IYY353 pKa = 11.08 LPPEE357 pKa = 4.15 NMGDD361 pKa = 3.39 HH362 pKa = 6.27 YY363 pKa = 11.65 LVITTVDD370 pKa = 3.22 AANPSAVVDD379 pKa = 4.28 QKK381 pKa = 11.7 AILCNGGSVYY391 pKa = 10.12 MSQDD395 pKa = 3.36 SIFLYY400 pKa = 8.58 EE401 pKa = 3.93 TTGYY405 pKa = 10.01 NWGIMPIARR414 pKa = 11.84 FAADD418 pKa = 5.26 DD419 pKa = 3.64 MAEE422 pKa = 4.31 PAGEE426 pKa = 3.98 TDD428 pKa = 5.17 PIAEE432 pKa = 4.84 AATEE436 pKa = 4.23 DD437 pKa = 4.46 PGDD440 pKa = 3.94 APADD444 pKa = 3.43 EE445 pKa = 4.82 SEE447 pKa = 4.48 NGEE450 pKa = 4.23 EE451 pKa = 4.44 APGATSPAEE460 pKa = 4.34 GPDD463 pKa = 3.81 GEE465 pKa = 5.13 GVNDD469 pKa = 4.71 DD470 pKa = 4.52 PAVDD474 pKa = 5.09 DD475 pKa = 4.76 PATLPADD482 pKa = 4.36 DD483 pKa = 5.26 PATLPADD490 pKa = 4.19 GPAAEE495 pKa = 4.49 PQSPEE500 pKa = 4.12 TPDD503 pKa = 3.77 EE504 pKa = 4.12 EE505 pKa = 4.45 AQAPSDD511 pKa = 3.91 NSADD515 pKa = 3.74 DD516 pKa = 4.08 PSEE519 pKa = 4.98 GEE521 pKa = 4.11 ADD523 pKa = 3.85 TPDD526 pKa = 3.45 SSGGDD531 pKa = 3.57 DD532 pKa = 4.85 PDD534 pKa = 4.23 LKK536 pKa = 10.02 TCIRR540 pKa = 11.84 KK541 pKa = 8.06 FTFDD545 pKa = 3.21 KK546 pKa = 11.24 GQLEE550 pKa = 4.47 GVAQGEE556 pKa = 4.78 VNGTLDD562 pKa = 4.42 SSFCLDD568 pKa = 3.79 EE569 pKa = 4.25 YY570 pKa = 11.28 NGYY573 pKa = 10.02 LRR575 pKa = 11.84 VVTTVYY581 pKa = 11.21 GEE583 pKa = 4.27 DD584 pKa = 3.34 TSSALTVLNEE594 pKa = 3.78 QMEE597 pKa = 4.72 EE598 pKa = 3.9 VGNIADD604 pKa = 3.86 VAPGEE609 pKa = 4.26 QVYY612 pKa = 9.88 SARR615 pKa = 11.84 FMGNVGYY622 pKa = 9.93 FVTFKK627 pKa = 10.84 QVDD630 pKa = 3.6 PLFSVDD636 pKa = 4.92 LSNPEE641 pKa = 3.77 NPTIIGQLKK650 pKa = 9.15 IPGFSEE656 pKa = 4.05 YY657 pKa = 10.73 LHH659 pKa = 7.02 PYY661 pKa = 9.62 GEE663 pKa = 4.14 NLLLGIGMSADD674 pKa = 3.52 DD675 pKa = 5.02 AGATDD680 pKa = 4.4 GVKK683 pKa = 10.78 LSMFDD688 pKa = 3.28 TSNPADD694 pKa = 3.54 VRR696 pKa = 11.84 EE697 pKa = 4.21 VATAVLDD704 pKa = 3.48 DD705 pKa = 4.4 TYY707 pKa = 11.68 YY708 pKa = 11.18 SDD710 pKa = 3.95 VFNEE714 pKa = 3.58 YY715 pKa = 9.81 RR716 pKa = 11.84 AALIDD721 pKa = 4.08 PASDD725 pKa = 3.4 MIGFPVSTDD734 pKa = 2.98 RR735 pKa = 11.84 EE736 pKa = 4.25 AYY738 pKa = 9.4 AVYY741 pKa = 9.88 GYY743 pKa = 10.67 SEE745 pKa = 5.35 DD746 pKa = 3.9 NGFSQHH752 pKa = 5.82 MLEE755 pKa = 4.41 EE756 pKa = 4.57 TNGTGWVPTRR766 pKa = 11.84 GIRR769 pKa = 11.84 IGDD772 pKa = 3.33 VLYY775 pKa = 10.79 VVKK778 pKa = 10.85 GNAAEE783 pKa = 4.25 SYY785 pKa = 10.61 RR786 pKa = 11.84 IGDD789 pKa = 3.59 YY790 pKa = 10.99 QKK792 pKa = 10.6 IDD794 pKa = 3.63 DD795 pKa = 4.63 LLII798 pKa = 4.67
Molecular weight: 84.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.897
Patrickios 1.545
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A3N0ABY4|A0A3N0ABY4_9ACTN 10 kDa chaperonin OS=Adlercreutzia caecicola OX=747645 GN=groS PE=3 SV=1
MM1 pKa = 7.21 TGGVGVIFLGVAVALAAVAGFGLAIGASALMRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 LRR38 pKa = 11.84 QTVATSAAAQGLGLSRR54 pKa = 11.84 GASCVPGGARR64 pKa = 11.84 AAPAVALLQAMEE76 pKa = 4.23 LRR78 pKa = 11.84 SRR80 pKa = 11.84 ALSMGARR87 pKa = 11.84 HH88 pKa = 6.25 PLVPARR94 pKa = 11.84 LLPAAASLEE103 pKa = 4.2 KK104 pKa = 10.45 AISHH108 pKa = 6.79 AGLAGAVTVAGFWDD122 pKa = 4.36 CSLLAALAGAVAGGLAGAAFSVEE145 pKa = 3.85 LALIGLVAGGFAGFRR160 pKa = 11.84 LPRR163 pKa = 11.84 AVLARR168 pKa = 11.84 AVQKK172 pKa = 10.73 RR173 pKa = 11.84 ADD175 pKa = 3.69 DD176 pKa = 4.11 LEE178 pKa = 5.74 RR179 pKa = 11.84 SLSEE183 pKa = 3.81 MLEE186 pKa = 4.26 VVALGLRR193 pKa = 11.84 SGLSFEE199 pKa = 4.78 GALALYY205 pKa = 7.49 TAHH208 pKa = 6.94 FEE210 pKa = 4.48 CPLATGMAAAQAQWSCGLVSRR231 pKa = 11.84 EE232 pKa = 3.56 EE233 pKa = 4.01 ALRR236 pKa = 11.84 ALAASYY242 pKa = 10.74 RR243 pKa = 11.84 SLLFGRR249 pKa = 11.84 VVEE252 pKa = 4.63 TIVRR256 pKa = 11.84 SIRR259 pKa = 11.84 FGSSLADD266 pKa = 3.67 NLDD269 pKa = 3.71 DD270 pKa = 4.45 AAAEE274 pKa = 4.01 ARR276 pKa = 11.84 AHH278 pKa = 5.8 YY279 pKa = 7.96 RR280 pKa = 11.84 TARR283 pKa = 11.84 QEE285 pKa = 4.4 SVAKK289 pKa = 10.65 APVKK293 pKa = 10.36 MMLPTSTLILPAMLILVLGPVLLEE317 pKa = 4.14 LMGG320 pKa = 4.35
Molecular weight: 32.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.326
IPC_protein 10.262
Toseland 10.379
ProMoST 10.189
Dawson 10.511
Bjellqvist 10.306
Wikipedia 10.76
Rodwell 10.511
Grimsley 10.57
Solomon 10.657
Lehninger 10.628
Nozaki 10.438
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.804
Sillero 10.467
Patrickios 10.233
IPC_peptide 10.657
IPC2_peptide 9.663
IPC2.peptide.svr19 8.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1821
0
1821
688622
32
26543
378.2
41.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.038 ± 0.105
1.61 ± 0.065
5.705 ± 0.128
6.629 ± 0.056
3.81 ± 0.055
8.379 ± 0.063
1.663 ± 0.028
4.655 ± 0.085
3.724 ± 0.044
9.26 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.634 ± 0.052
3.148 ± 0.137
4.76 ± 0.043
3.514 ± 0.029
5.527 ± 0.089
5.575 ± 0.048
5.283 ± 0.157
7.97 ± 0.055
1.21 ± 0.03
2.906 ± 0.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here