Sphingomonadales bacterium EhC05

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; unclassified Sphingomonadales

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B6Z835|A0A1B6Z835_9SPHN Transport permease protein OS=Sphingomonadales bacterium EhC05 OX=1849171 GN=A8B75_16020 PE=3 SV=1
MM1 pKa = 7.79SGFLCLCLVALFPAEE16 pKa = 3.96KK17 pKa = 10.41AKK19 pKa = 10.94AQTITTYY26 pKa = 11.13SNSSTIAIPDD36 pKa = 3.69NACPATVNRR45 pKa = 11.84TFSVGSSYY53 pKa = 11.24EE54 pKa = 3.95VGDD57 pKa = 3.54VDD59 pKa = 5.01IGILLDD65 pKa = 3.15HH66 pKa = 7.06TYY68 pKa = 10.91RR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.47LEE73 pKa = 4.2ITLTSPTGTIVTLMDD88 pKa = 3.95FVGGGANNLNVRR100 pKa = 11.84FDD102 pKa = 4.24DD103 pKa = 4.11EE104 pKa = 4.64ATGGTIASHH113 pKa = 6.36TSSDD117 pKa = 3.98PLTPIYY123 pKa = 10.74NSTKK127 pKa = 9.81IPQNSLSAFDD137 pKa = 5.38GEE139 pKa = 4.68DD140 pKa = 3.59AQGTWTLRR148 pKa = 11.84ICDD151 pKa = 3.52QQGIDD156 pKa = 3.27VGSFRR161 pKa = 11.84QADD164 pKa = 4.22LYY166 pKa = 9.88ITQLPPFADD175 pKa = 3.76LSLTKK180 pKa = 10.65SFGATSSNSGTYY192 pKa = 10.0SLSVTNSTISALTATGVTVSDD213 pKa = 3.95TLPSGVSLTGISGTGTYY230 pKa = 10.29SGGVWTLGTAIAPGQTVSIEE250 pKa = 3.95LSVTITATSGTITNSAQIRR269 pKa = 11.84SSTAFDD275 pKa = 4.08SDD277 pKa = 3.78STPNNGVTSEE287 pKa = 4.53DD288 pKa = 4.2DD289 pKa = 3.64YY290 pKa = 12.01ASVSFTAGTRR300 pKa = 11.84LPGYY304 pKa = 9.74VPPLTNVCPISNQLQFSWGSPTTWNPSGSLYY335 pKa = 9.1EE336 pKa = 4.37TYY338 pKa = 10.1TVTGIGEE345 pKa = 4.07IEE347 pKa = 4.05FTIAEE352 pKa = 4.28PPGGFTNSTPEE363 pKa = 3.64ITTSNTGGGAAGTRR377 pKa = 11.84ALYY380 pKa = 10.52FFMNNGNEE388 pKa = 4.28THH390 pKa = 6.71SSSTTLEE397 pKa = 4.33LPTAVPGLQFTIFDD411 pKa = 3.66IDD413 pKa = 3.67FGNNQFTDD421 pKa = 3.5MVTVTGEE428 pKa = 3.87FDD430 pKa = 3.37GATVLPTLSNNTANYY445 pKa = 9.75ISGNSAIGDD454 pKa = 3.81GSSSGTQEE462 pKa = 3.77FGNVVVTFTSPVDD475 pKa = 3.59SVTIVYY481 pKa = 10.54GNYY484 pKa = 9.63QPTTPTSSGNQAMSIHH500 pKa = 7.44DD501 pKa = 3.68FTFCKK506 pKa = 10.43PEE508 pKa = 3.86TTISITKK515 pKa = 10.19LSEE518 pKa = 3.91VLNDD522 pKa = 4.91PISPADD528 pKa = 3.1KK529 pKa = 9.93WKK531 pKa = 10.41RR532 pKa = 11.84IPDD535 pKa = 3.45ATVQYY540 pKa = 10.89CLFISNSGSGTAEE553 pKa = 3.71NVTVTDD559 pKa = 3.73NLVGPFTYY567 pKa = 9.75IAEE570 pKa = 4.38SMNSGANCDD579 pKa = 3.6SANTFEE585 pKa = 6.51DD586 pKa = 4.57DD587 pKa = 4.19DD588 pKa = 5.53SSDD591 pKa = 3.49GTEE594 pKa = 4.17TDD596 pKa = 5.38DD597 pKa = 3.54ITASIAGSTITVTAEE612 pKa = 4.3KK613 pKa = 10.13IGPTDD618 pKa = 3.25NFALTFRR625 pKa = 11.84VTIDD629 pKa = 2.85

Molecular weight: 65.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B6Z791|A0A1B6Z791_9SPHN AB hydrolase-1 domain-containing protein OS=Sphingomonadales bacterium EhC05 OX=1849171 GN=A8B75_16870 PE=4 SV=1
MM1 pKa = 7.24TKK3 pKa = 9.49ATIAVSPLRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84MIDD17 pKa = 3.98DD18 pKa = 3.19MSLRR22 pKa = 11.84NLSPATQRR30 pKa = 11.84SYY32 pKa = 11.46LHH34 pKa = 6.74AVTKK38 pKa = 9.71FSRR41 pKa = 11.84FYY43 pKa = 11.2GRR45 pKa = 11.84SPDD48 pKa = 3.76CLGLEE53 pKa = 4.44DD54 pKa = 3.86VRR56 pKa = 11.84AFQVHH61 pKa = 6.25LVSQGISWGSLNQIVCALRR80 pKa = 11.84FFYY83 pKa = 10.57GVTLDD88 pKa = 3.41RR89 pKa = 11.84GEE91 pKa = 3.83IPKK94 pKa = 10.26RR95 pKa = 11.84IVYY98 pKa = 10.65ARR100 pKa = 11.84MPRR103 pKa = 11.84KK104 pKa = 10.02LPTILSADD112 pKa = 3.51EE113 pKa = 3.97VVRR116 pKa = 11.84FLEE119 pKa = 4.58AVPSLKK125 pKa = 10.44ARR127 pKa = 11.84AALTTAYY134 pKa = 10.36AAGLRR139 pKa = 11.84ASEE142 pKa = 4.32VASLRR147 pKa = 11.84VTDD150 pKa = 3.8IDD152 pKa = 3.85ADD154 pKa = 3.84RR155 pKa = 11.84MVIQIHH161 pKa = 6.13HH162 pKa = 6.67GKK164 pKa = 9.17VAQDD168 pKa = 3.37RR169 pKa = 11.84TVMLSPQLLAILRR182 pKa = 11.84TYY184 pKa = 9.07WRR186 pKa = 11.84LARR189 pKa = 11.84PPEE192 pKa = 4.14CLFPGRR198 pKa = 11.84TPSKK202 pKa = 9.9PIAITVLHH210 pKa = 6.34AACRR214 pKa = 11.84SATKK218 pKa = 10.36AAGLTKK224 pKa = 10.06RR225 pKa = 11.84VSVHH229 pKa = 4.88TLRR232 pKa = 11.84HH233 pKa = 5.12SFATHH238 pKa = 6.31LLEE241 pKa = 5.11SGVDD245 pKa = 3.19IRR247 pKa = 11.84IIQVLLGHH255 pKa = 6.84RR256 pKa = 11.84NLSTTALYY264 pKa = 8.74TQVATSTIAATQSPLDD280 pKa = 3.72RR281 pKa = 11.84LALEE285 pKa = 4.53VVPPTT290 pKa = 3.92

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3899

0

3899

1226322

40

3577

314.5

34.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.072 ± 0.046

0.827 ± 0.013

6.27 ± 0.028

5.901 ± 0.038

3.943 ± 0.024

8.268 ± 0.052

1.956 ± 0.02

6.238 ± 0.027

4.207 ± 0.036

9.527 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.022

3.42 ± 0.031

4.659 ± 0.028

3.556 ± 0.022

5.877 ± 0.038

6.199 ± 0.035

5.163 ± 0.031

6.486 ± 0.035

1.384 ± 0.016

2.396 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski