Amino acid dipepetide frequency for Sphingomonadales bacterium EhC05

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.536AlaAla: 13.536 ± 0.147	0.905AlaCys: 0.905 ± 0.03	6.727AlaAsp: 6.727 ± 0.086	7.035AlaGlu: 7.035 ± 0.097	3.939AlaPhe: 3.939 ± 0.059	8.919AlaGly: 8.919 ± 0.132	1.967AlaHis: 1.967 ± 0.047	7.221AlaIle: 7.221 ± 0.091	5.013AlaLys: 5.013 ± 0.083	11.004AlaLeu: 11.004 ± 0.127	3.444AlaMet: 3.444 ± 0.059	3.659AlaAsn: 3.659 ± 0.071	4.456AlaPro: 4.456 ± 0.067	4.186AlaGln: 4.186 ± 0.064	6.23AlaArg: 6.23 ± 0.088	6.301AlaSer: 6.301 ± 0.082	5.52AlaThr: 5.52 ± 0.096	7.044AlaVal: 7.044 ± 0.091	1.213AlaTrp: 1.213 ± 0.034	2.399AlaTyr: 2.399 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.848CysAla: 0.848 ± 0.03	0.11CysCys: 0.11 ± 0.01	0.59CysAsp: 0.59 ± 0.021	0.474CysGlu: 0.474 ± 0.019	0.316CysPhe: 0.316 ± 0.015	0.842CysGly: 0.842 ± 0.029	0.236CysHis: 0.236 ± 0.016	0.418CysIle: 0.418 ± 0.019	0.255CysLys: 0.255 ± 0.015	0.758CysLeu: 0.758 ± 0.026	0.161CysMet: 0.161 ± 0.012	0.285CysAsn: 0.285 ± 0.015	0.456CysPro: 0.456 ± 0.022	0.272CysGln: 0.272 ± 0.014	0.5CysArg: 0.5 ± 0.02	0.559CysSer: 0.559 ± 0.021	0.369CysThr: 0.369 ± 0.017	0.522CysVal: 0.522 ± 0.021	0.112CysTrp: 0.112 ± 0.009	0.183CysTyr: 0.183 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.349AspAla: 6.349 ± 0.086	0.534AspCys: 0.534 ± 0.021	3.917AspAsp: 3.917 ± 0.063	3.981AspGlu: 3.981 ± 0.071	2.484AspPhe: 2.484 ± 0.05	5.635AspGly: 5.635 ± 0.086	1.413AspHis: 1.413 ± 0.041	4.106AspIle: 4.106 ± 0.067	2.405AspLys: 2.405 ± 0.049	5.651AspLeu: 5.651 ± 0.067	1.749AspMet: 1.749 ± 0.038	1.997AspAsn: 1.997 ± 0.044	3.356AspPro: 3.356 ± 0.058	2.496AspGln: 2.496 ± 0.045	4.168AspArg: 4.168 ± 0.067	3.056AspSer: 3.056 ± 0.046	2.422AspThr: 2.422 ± 0.047	4.07AspVal: 4.07 ± 0.052	1.092AspTrp: 1.092 ± 0.031	1.816AspTyr: 1.816 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.767GluAla: 6.767 ± 0.097	0.334GluCys: 0.334 ± 0.018	3.286GluAsp: 3.286 ± 0.059	3.519GluGlu: 3.519 ± 0.076	2.049GluPhe: 2.049 ± 0.044	4.076GluGly: 4.076 ± 0.062	1.179GluHis: 1.179 ± 0.032	4.054GluIle: 4.054 ± 0.064	3.051GluLys: 3.051 ± 0.064	5.515GluLeu: 5.515 ± 0.072	1.82GluMet: 1.82 ± 0.04	2.305GluAsn: 2.305 ± 0.043	2.44GluPro: 2.44 ± 0.057	2.585GluGln: 2.585 ± 0.049	3.979GluArg: 3.979 ± 0.065	2.963GluSer: 2.963 ± 0.048	3.538GluThr: 3.538 ± 0.054	3.392GluVal: 3.392 ± 0.054	0.756GluTrp: 0.756 ± 0.022	1.405GluTyr: 1.405 ± 0.033	0.001GluXaa: 0.001 ± 0.001
Phe	4.622PheAla: 4.622 ± 0.061	0.387PheCys: 0.387 ± 0.02	3.012PheAsp: 3.012 ± 0.056	2.415PheGlu: 2.415 ± 0.047	1.557PhePhe: 1.557 ± 0.039	3.903PheGly: 3.903 ± 0.068	0.764PheHis: 0.764 ± 0.026	2.027PheIle: 2.027 ± 0.044	1.269PheLys: 1.269 ± 0.034	3.515PheLeu: 3.515 ± 0.066	0.881PheMet: 0.881 ± 0.031	1.306PheAsn: 1.306 ± 0.043	1.582PhePro: 1.582 ± 0.041	1.133PheGln: 1.133 ± 0.03	2.032PheArg: 2.032 ± 0.037	2.626PheSer: 2.626 ± 0.049	2.075PheThr: 2.075 ± 0.045	2.716PheVal: 2.716 ± 0.051	0.625PheTrp: 0.625 ± 0.023	0.986PheTyr: 0.986 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.053GlyAla: 8.053 ± 0.133	0.798GlyCys: 0.798 ± 0.028	4.919GlyAsp: 4.919 ± 0.106	4.601GlyGlu: 4.601 ± 0.069	3.727GlyPhe: 3.727 ± 0.058	7.337GlyGly: 7.337 ± 0.248	1.646GlyHis: 1.646 ± 0.045	4.832GlyIle: 4.832 ± 0.073	4.011GlyLys: 4.011 ± 0.057	8.101GlyLeu: 8.101 ± 0.097	2.205GlyMet: 2.205 ± 0.047	2.786GlyAsn: 2.786 ± 0.095	3.169GlyPro: 3.169 ± 0.051	3.165GlyGln: 3.165 ± 0.05	4.771GlyArg: 4.771 ± 0.061	5.295GlySer: 5.295 ± 0.1	4.464GlyThr: 4.464 ± 0.11	5.071GlyVal: 5.071 ± 0.071	1.411GlyTrp: 1.411 ± 0.038	2.322GlyTyr: 2.322 ± 0.042	0.001GlyXaa: 0.001 ± 0.001
His	1.782HisAla: 1.782 ± 0.04	0.25HisCys: 0.25 ± 0.016	1.235HisAsp: 1.235 ± 0.033	1.023HisGlu: 1.023 ± 0.032	0.88HisPhe: 0.88 ± 0.029	1.738HisGly: 1.738 ± 0.04	0.599HisHis: 0.599 ± 0.026	1.257HisIle: 1.257 ± 0.032	0.736HisLys: 0.736 ± 0.027	1.867HisLeu: 1.867 ± 0.04	0.5HisMet: 0.5 ± 0.021	0.581HisAsn: 0.581 ± 0.02	1.191HisPro: 1.191 ± 0.035	0.714HisGln: 0.714 ± 0.027	1.233HisArg: 1.233 ± 0.034	1.153HisSer: 1.153 ± 0.034	0.601HisThr: 0.601 ± 0.024	1.284HisVal: 1.284 ± 0.035	0.364HisTrp: 0.364 ± 0.017	0.571HisTyr: 0.571 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.001IleAla: 8.001 ± 0.09	0.637IleCys: 0.637 ± 0.024	4.412IleAsp: 4.412 ± 0.061	3.955IleGlu: 3.955 ± 0.062	2.567IlePhe: 2.567 ± 0.051	5.623IleGly: 5.623 ± 0.083	0.983IleHis: 0.983 ± 0.029	3.656IleIle: 3.656 ± 0.065	2.114IleLys: 2.114 ± 0.039	5.511IleLeu: 5.511 ± 0.073	1.365IleMet: 1.365 ± 0.032	2.105IleAsn: 2.105 ± 0.054	2.688IlePro: 2.688 ± 0.055	1.542IleGln: 1.542 ± 0.036	3.138IleArg: 3.138 ± 0.049	4.241IleSer: 4.241 ± 0.07	3.161IleThr: 3.161 ± 0.063	4.268IleVal: 4.268 ± 0.069	0.916IleTrp: 0.916 ± 0.028	1.495IleTyr: 1.495 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	4.692LysAla: 4.692 ± 0.076	0.272LysCys: 0.272 ± 0.018	2.217LysAsp: 2.217 ± 0.045	1.942LysGlu: 1.942 ± 0.046	1.4LysPhe: 1.4 ± 0.031	2.892LysGly: 2.892 ± 0.052	0.75LysHis: 0.75 ± 0.024	2.752LysIle: 2.752 ± 0.052	2.141LysLys: 2.141 ± 0.055	4.386LysLeu: 4.386 ± 0.064	1.294LysMet: 1.294 ± 0.036	1.522LysAsn: 1.522 ± 0.035	2.311LysPro: 2.311 ± 0.051	1.566LysGln: 1.566 ± 0.042	2.688LysArg: 2.688 ± 0.046	2.809LysSer: 2.809 ± 0.06	2.379LysThr: 2.379 ± 0.048	2.476LysVal: 2.476 ± 0.043	0.582LysTrp: 0.582 ± 0.024	0.996LysTyr: 0.996 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	11.334LeuAla: 11.334 ± 0.119	0.755LeuCys: 0.755 ± 0.026	5.877LeuAsp: 5.877 ± 0.077	5.486LeuGlu: 5.486 ± 0.074	3.914LeuPhe: 3.914 ± 0.069	7.571LeuGly: 7.571 ± 0.091	1.726LeuHis: 1.726 ± 0.038	5.829LeuIle: 5.829 ± 0.083	3.802LeuLys: 3.802 ± 0.073	8.865LeuLeu: 8.865 ± 0.126	2.233LeuMet: 2.233 ± 0.052	3.157LeuAsn: 3.157 ± 0.057	4.727LeuPro: 4.727 ± 0.067	2.9LeuGln: 2.9 ± 0.051	5.337LeuArg: 5.337 ± 0.061	6.689LeuSer: 6.689 ± 0.086	5.441LeuThr: 5.441 ± 0.09	6.446LeuVal: 6.446 ± 0.076	1.178LeuTrp: 1.178 ± 0.038	2.002LeuTyr: 2.002 ± 0.043	0.001LeuXaa: 0.001 ± 0.001
Met	3.101MetAla: 3.101 ± 0.057	0.189MetCys: 0.189 ± 0.012	1.434MetAsp: 1.434 ± 0.036	1.329MetGlu: 1.329 ± 0.039	0.798MetPhe: 0.798 ± 0.028	1.971MetGly: 1.971 ± 0.046	0.469MetHis: 0.469 ± 0.02	1.771MetIle: 1.771 ± 0.039	1.246MetLys: 1.246 ± 0.035	2.631MetLeu: 2.631 ± 0.057	0.796MetMet: 0.796 ± 0.026	0.929MetAsn: 0.929 ± 0.027	1.412MetPro: 1.412 ± 0.038	0.943MetGln: 0.943 ± 0.025	1.618MetArg: 1.618 ± 0.042	1.67MetSer: 1.67 ± 0.04	1.94MetThr: 1.94 ± 0.037	1.724MetVal: 1.724 ± 0.041	0.238MetTrp: 0.238 ± 0.015	0.291MetTyr: 0.291 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.555AsnAla: 3.555 ± 0.08	0.333AsnCys: 0.333 ± 0.018	2.017AsnAsp: 2.017 ± 0.044	1.631AsnGlu: 1.631 ± 0.037	1.45AsnPhe: 1.45 ± 0.037	3.058AsnGly: 3.058 ± 0.076	0.674AsnHis: 0.674 ± 0.021	2.442AsnIle: 2.442 ± 0.061	1.113AsnLys: 1.113 ± 0.032	3.374AsnLeu: 3.374 ± 0.059	0.922AsnMet: 0.922 ± 0.026	1.158AsnAsn: 1.158 ± 0.039	2.102AsnPro: 2.102 ± 0.043	1.18AsnGln: 1.18 ± 0.031	2.142AsnArg: 2.142 ± 0.047	2.055AsnSer: 2.055 ± 0.05	1.284AsnThr: 1.284 ± 0.039	2.242AsnVal: 2.242 ± 0.059	0.592AsnTrp: 0.592 ± 0.027	0.877AsnTyr: 0.877 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.893ProAla: 4.893 ± 0.069	0.274ProCys: 0.274 ± 0.015	3.703ProAsp: 3.703 ± 0.062	3.618ProGlu: 3.618 ± 0.048	1.886ProPhe: 1.886 ± 0.037	3.588ProGly: 3.588 ± 0.06	0.867ProHis: 0.867 ± 0.027	2.791ProIle: 2.791 ± 0.049	2.083ProLys: 2.083 ± 0.046	4.172ProLeu: 4.172 ± 0.067	1.183ProMet: 1.183 ± 0.033	1.569ProAsn: 1.569 ± 0.037	2.009ProPro: 2.009 ± 0.061	1.584ProGln: 1.584 ± 0.038	2.072ProArg: 2.072 ± 0.045	2.729ProSer: 2.729 ± 0.047	2.309ProThr: 2.309 ± 0.048	3.647ProVal: 3.647 ± 0.066	0.552ProTrp: 0.552 ± 0.024	1.064ProTyr: 1.064 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.968GlnAla: 3.968 ± 0.063	0.287GlnCys: 0.287 ± 0.017	1.862GlnAsp: 1.862 ± 0.04	1.707GlnGlu: 1.707 ± 0.04	1.458GlnPhe: 1.458 ± 0.033	2.388GlnGly: 2.388 ± 0.038	0.688GlnHis: 0.688 ± 0.022	2.51GlnIle: 2.51 ± 0.047	1.633GlnLys: 1.633 ± 0.04	3.524GlnLeu: 3.524 ± 0.05	1.014GlnMet: 1.014 ± 0.032	1.359GlnAsn: 1.359 ± 0.034	1.654GlnPro: 1.654 ± 0.04	1.478GlnGln: 1.478 ± 0.037	2.264GlnArg: 2.264 ± 0.041	2.416GlnSer: 2.416 ± 0.045	1.851GlnThr: 1.851 ± 0.039	2.103GlnVal: 2.103 ± 0.042	0.52GlnTrp: 0.52 ± 0.02	0.878GlnTyr: 0.878 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	5.813ArgAla: 5.813 ± 0.077	0.436ArgCys: 0.436 ± 0.019	3.599ArgAsp: 3.599 ± 0.063	3.517ArgGlu: 3.517 ± 0.058	2.657ArgPhe: 2.657 ± 0.05	3.82ArgGly: 3.82 ± 0.059	1.287ArgHis: 1.287 ± 0.035	3.681ArgIle: 3.681 ± 0.061	2.743ArgLys: 2.743 ± 0.045	6.222ArgLeu: 6.222 ± 0.081	1.598ArgMet: 1.598 ± 0.041	2.164ArgAsn: 2.164 ± 0.043	2.633ArgPro: 2.633 ± 0.055	2.259ArgGln: 2.259 ± 0.047	3.67ArgArg: 3.67 ± 0.065	3.624ArgSer: 3.624 ± 0.057	2.885ArgThr: 2.885 ± 0.05	3.529ArgVal: 3.529 ± 0.056	0.963ArgTrp: 0.963 ± 0.03	1.67ArgTyr: 1.67 ± 0.04	0.001ArgXaa: 0.001 ± 0.001
Ser	6.559SerAla: 6.559 ± 0.087	0.506SerCys: 0.506 ± 0.021	4.08SerAsp: 4.08 ± 0.062	3.638SerGlu: 3.638 ± 0.059	2.574SerPhe: 2.574 ± 0.048	6.114SerGly: 6.114 ± 0.102	1.172SerHis: 1.172 ± 0.035	3.702SerIle: 3.702 ± 0.061	2.507SerLys: 2.507 ± 0.049	5.82SerLeu: 5.82 ± 0.073	1.513SerMet: 1.513 ± 0.038	2.089SerAsn: 2.089 ± 0.05	2.797SerPro: 2.797 ± 0.053	2.154SerGln: 2.154 ± 0.046	3.539SerArg: 3.539 ± 0.057	3.894SerSer: 3.894 ± 0.063	2.826SerThr: 2.826 ± 0.056	4.021SerVal: 4.021 ± 0.067	0.865SerTrp: 0.865 ± 0.023	1.624SerTyr: 1.624 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	5.782ThrAla: 5.782 ± 0.08	0.392ThrCys: 0.392 ± 0.019	3.139ThrAsp: 3.139 ± 0.062	2.83ThrGlu: 2.83 ± 0.054	1.834ThrPhe: 1.834 ± 0.037	5.094ThrGly: 5.094 ± 0.104	1.005ThrHis: 1.005 ± 0.029	3.347ThrIle: 3.347 ± 0.062	2.003ThrLys: 2.003 ± 0.045	5.019ThrLeu: 5.019 ± 0.064	1.244ThrMet: 1.244 ± 0.03	1.749ThrAsn: 1.749 ± 0.044	2.759ThrPro: 2.759 ± 0.05	1.628ThrGln: 1.628 ± 0.041	2.737ThrArg: 2.737 ± 0.056	3.114ThrSer: 3.114 ± 0.068	2.611ThrThr: 2.611 ± 0.066	3.704ThrVal: 3.704 ± 0.062	0.547ThrTrp: 0.547 ± 0.024	1.094ThrTyr: 1.094 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.453ValAla: 7.453 ± 0.091	0.481ValCys: 0.481 ± 0.021	4.244ValAsp: 4.244 ± 0.054	4.341ValGlu: 4.341 ± 0.059	2.238ValPhe: 2.238 ± 0.044	5.079ValGly: 5.079 ± 0.094	1.249ValHis: 1.249 ± 0.033	4.006ValIle: 4.006 ± 0.067	2.557ValLys: 2.557 ± 0.055	5.351ValLeu: 5.351 ± 0.069	1.68ValMet: 1.68 ± 0.044	2.26ValAsn: 2.26 ± 0.055	3.13ValPro: 3.13 ± 0.049	2.192ValGln: 2.192 ± 0.041	3.907ValArg: 3.907 ± 0.063	4.342ValSer: 4.342 ± 0.068	4.087ValThr: 4.087 ± 0.076	4.247ValVal: 4.247 ± 0.07	0.684ValTrp: 0.684 ± 0.02	1.329ValTyr: 1.329 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.151TrpAla: 1.151 ± 0.036	0.123TrpCys: 0.123 ± 0.011	0.722TrpAsp: 0.722 ± 0.023	0.674TrpGlu: 0.674 ± 0.025	0.59TrpPhe: 0.59 ± 0.024	0.908TrpGly: 0.908 ± 0.027	0.325TrpHis: 0.325 ± 0.014	0.772TrpIle: 0.772 ± 0.026	0.594TrpLys: 0.594 ± 0.022	1.635TrpLeu: 1.635 ± 0.043	0.404TrpMet: 0.404 ± 0.017	0.501TrpAsn: 0.501 ± 0.02	0.627TrpPro: 0.627 ± 0.023	0.65TrpGln: 0.65 ± 0.023	1.031TrpArg: 1.031 ± 0.034	0.969TrpSer: 0.969 ± 0.029	0.834TrpThr: 0.834 ± 0.028	0.837TrpVal: 0.837 ± 0.026	0.222TrpTrp: 0.222 ± 0.013	0.276TrpTyr: 0.276 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.459TyrAla: 2.459 ± 0.046	0.264TyrCys: 0.264 ± 0.015	1.704TyrAsp: 1.704 ± 0.039	1.297TyrGlu: 1.297 ± 0.034	1.114TyrPhe: 1.114 ± 0.034	2.125TyrGly: 2.125 ± 0.041	0.559TyrHis: 0.559 ± 0.022	1.204TyrIle: 1.204 ± 0.033	0.792TyrLys: 0.792 ± 0.028	2.349TyrLeu: 2.349 ± 0.044	0.493TyrMet: 0.493 ± 0.021	0.722TyrAsn: 0.722 ± 0.026	1.096TyrPro: 1.096 ± 0.032	0.937TyrGln: 0.937 ± 0.028	1.715TyrArg: 1.715 ± 0.04	1.489TyrSer: 1.489 ± 0.031	1.032TyrThr: 1.032 ± 0.029	1.515TyrVal: 1.515 ± 0.037	0.413TyrTrp: 0.413 ± 0.019	0.684TyrTyr: 0.684 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3899 proteins (1226322 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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