Amino acid dipepetide frequency for Pichia membranifaciens NRRL Y-2026

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.409AlaAla: 5.409 ± 0.085	0.809AlaCys: 0.809 ± 0.019	3.499AlaAsp: 3.499 ± 0.042	3.893AlaGlu: 3.893 ± 0.051	2.443AlaPhe: 2.443 ± 0.038	3.765AlaGly: 3.765 ± 0.056	1.179AlaHis: 1.179 ± 0.022	3.583AlaIle: 3.583 ± 0.037	4.174AlaLys: 4.174 ± 0.049	5.51AlaLeu: 5.51 ± 0.05	1.233AlaMet: 1.233 ± 0.023	3.156AlaAsn: 3.156 ± 0.041	2.497AlaPro: 2.497 ± 0.047	2.072AlaGln: 2.072 ± 0.038	2.573AlaArg: 2.573 ± 0.035	5.495AlaSer: 5.495 ± 0.053	3.635AlaThr: 3.635 ± 0.045	3.751AlaVal: 3.751 ± 0.048	0.563AlaTrp: 0.563 ± 0.017	1.848AlaTyr: 1.848 ± 0.032	0.0AlaXaa: 0.0 ± 0.0
Cys	0.704CysAla: 0.704 ± 0.016	0.326CysCys: 0.326 ± 0.014	0.718CysAsp: 0.718 ± 0.019	0.649CysGlu: 0.649 ± 0.016	0.732CysPhe: 0.732 ± 0.017	0.872CysGly: 0.872 ± 0.022	0.305CysHis: 0.305 ± 0.012	0.915CysIle: 0.915 ± 0.018	0.728CysLys: 0.728 ± 0.017	1.345CysLeu: 1.345 ± 0.024	0.282CysMet: 0.282 ± 0.01	0.551CysAsn: 0.551 ± 0.016	0.516CysPro: 0.516 ± 0.018	0.387CysGln: 0.387 ± 0.012	0.52CysArg: 0.52 ± 0.015	1.08CysSer: 1.08 ± 0.022	0.661CysThr: 0.661 ± 0.017	0.888CysVal: 0.888 ± 0.02	0.18CysTrp: 0.18 ± 0.009	0.489CysTyr: 0.489 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.654AspAla: 3.654 ± 0.042	0.649AspCys: 0.649 ± 0.017	5.363AspAsp: 5.363 ± 0.085	5.234AspGlu: 5.234 ± 0.061	2.776AspPhe: 2.776 ± 0.032	3.362AspGly: 3.362 ± 0.042	1.11AspHis: 1.11 ± 0.018	3.917AspIle: 3.917 ± 0.046	3.557AspLys: 3.557 ± 0.037	5.729AspLeu: 5.729 ± 0.046	1.234AspMet: 1.234 ± 0.021	3.049AspAsn: 3.049 ± 0.039	2.525AspPro: 2.525 ± 0.032	1.764AspGln: 1.764 ± 0.03	2.249AspArg: 2.249 ± 0.033	5.188AspSer: 5.188 ± 0.056	3.071AspThr: 3.071 ± 0.033	3.748AspVal: 3.748 ± 0.04	0.64AspTrp: 0.64 ± 0.017	2.312AspTyr: 2.312 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	3.689GluAla: 3.689 ± 0.053	0.686GluCys: 0.686 ± 0.017	4.375GluAsp: 4.375 ± 0.046	5.954GluGlu: 5.954 ± 0.093	2.723GluPhe: 2.723 ± 0.035	2.901GluGly: 2.901 ± 0.034	1.217GluHis: 1.217 ± 0.022	4.279GluIle: 4.279 ± 0.046	5.658GluLys: 5.658 ± 0.074	6.332GluLeu: 6.332 ± 0.047	1.461GluMet: 1.461 ± 0.021	4.222GluAsn: 4.222 ± 0.041	1.952GluPro: 1.952 ± 0.026	2.408GluGln: 2.408 ± 0.033	3.001GluArg: 3.001 ± 0.037	4.943GluSer: 4.943 ± 0.058	3.489GluThr: 3.489 ± 0.04	3.535GluVal: 3.535 ± 0.034	0.65GluTrp: 0.65 ± 0.017	2.24GluTyr: 2.24 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	2.535PheAla: 2.535 ± 0.036	0.61PheCys: 0.61 ± 0.016	2.963PheAsp: 2.963 ± 0.032	2.857PheGlu: 2.857 ± 0.037	2.154PhePhe: 2.154 ± 0.036	2.76PheGly: 2.76 ± 0.045	0.95PheHis: 0.95 ± 0.019	2.708PheIle: 2.708 ± 0.037	3.027PheLys: 3.027 ± 0.034	4.067PheLeu: 4.067 ± 0.052	0.909PheMet: 0.909 ± 0.021	2.513PheAsn: 2.513 ± 0.032	1.719PhePro: 1.719 ± 0.025	1.666PheGln: 1.666 ± 0.026	1.737PheArg: 1.737 ± 0.026	3.831PheSer: 3.831 ± 0.047	2.386PheThr: 2.386 ± 0.028	2.823PheVal: 2.823 ± 0.04	0.54PheTrp: 0.54 ± 0.015	1.566PheTyr: 1.566 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	3.491GlyAla: 3.491 ± 0.054	0.813GlyCys: 0.813 ± 0.021	3.196GlyAsp: 3.196 ± 0.036	3.156GlyGlu: 3.156 ± 0.036	2.593GlyPhe: 2.593 ± 0.04	3.874GlyGly: 3.874 ± 0.066	1.15GlyHis: 1.15 ± 0.025	3.352GlyIle: 3.352 ± 0.039	3.81GlyLys: 3.81 ± 0.043	5.022GlyLeu: 5.022 ± 0.048	1.215GlyMet: 1.215 ± 0.027	2.982GlyAsn: 2.982 ± 0.043	1.767GlyPro: 1.767 ± 0.029	1.584GlyGln: 1.584 ± 0.031	2.404GlyArg: 2.404 ± 0.039	4.981GlySer: 4.981 ± 0.054	3.008GlyThr: 3.008 ± 0.04	3.54GlyVal: 3.54 ± 0.043	0.645GlyTrp: 0.645 ± 0.017	2.045GlyTyr: 2.045 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	1.142HisAla: 1.142 ± 0.022	0.296HisCys: 0.296 ± 0.012	1.189HisAsp: 1.189 ± 0.022	1.244HisGlu: 1.244 ± 0.023	0.915HisPhe: 0.915 ± 0.02	1.203HisGly: 1.203 ± 0.024	0.679HisHis: 0.679 ± 0.023	1.246HisIle: 1.246 ± 0.024	1.229HisLys: 1.229 ± 0.022	2.0HisLeu: 2.0 ± 0.03	0.441HisMet: 0.441 ± 0.013	1.117HisAsn: 1.117 ± 0.02	1.147HisPro: 1.147 ± 0.022	0.902HisGln: 0.902 ± 0.027	1.019HisArg: 1.019 ± 0.02	1.903HisSer: 1.903 ± 0.029	1.138HisThr: 1.138 ± 0.023	1.219HisVal: 1.219 ± 0.02	0.222HisTrp: 0.222 ± 0.01	0.801HisTyr: 0.801 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	3.474IleAla: 3.474 ± 0.038	0.886IleCys: 0.886 ± 0.019	4.14IleAsp: 4.14 ± 0.043	4.027IleGlu: 4.027 ± 0.05	2.724IlePhe: 2.724 ± 0.038	3.25IleGly: 3.25 ± 0.041	1.298IleHis: 1.298 ± 0.023	3.716IleIle: 3.716 ± 0.045	4.042IleLys: 4.042 ± 0.052	5.882IleLeu: 5.882 ± 0.062	1.287IleMet: 1.287 ± 0.023	3.491IleAsn: 3.491 ± 0.038	3.114IlePro: 3.114 ± 0.034	2.226IleGln: 2.226 ± 0.03	2.589IleArg: 2.589 ± 0.037	5.435IleSer: 5.435 ± 0.048	3.286IleThr: 3.286 ± 0.037	3.863IleVal: 3.863 ± 0.04	0.652IleTrp: 0.652 ± 0.016	2.121IleTyr: 2.121 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.857LysAla: 3.857 ± 0.049	0.784LysCys: 0.784 ± 0.021	3.963LysAsp: 3.963 ± 0.038	5.024LysGlu: 5.024 ± 0.06	3.033LysPhe: 3.033 ± 0.034	3.03LysGly: 3.03 ± 0.036	1.479LysHis: 1.479 ± 0.024	4.524LysIle: 4.524 ± 0.053	6.113LysLys: 6.113 ± 0.075	7.145LysLeu: 7.145 ± 0.061	1.506LysMet: 1.506 ± 0.026	4.009LysAsn: 4.009 ± 0.048	2.733LysPro: 2.733 ± 0.041	2.693LysGln: 2.693 ± 0.033	3.611LysArg: 3.611 ± 0.042	5.575LysSer: 5.575 ± 0.057	3.889LysThr: 3.889 ± 0.039	4.122LysVal: 4.122 ± 0.039	0.701LysTrp: 0.701 ± 0.018	2.633LysTyr: 2.633 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	5.737LeuAla: 5.737 ± 0.053	1.274LeuCys: 1.274 ± 0.021	5.722LeuAsp: 5.722 ± 0.047	5.894LeuGlu: 5.894 ± 0.053	4.13LeuPhe: 4.13 ± 0.048	4.785LeuGly: 4.785 ± 0.049	2.031LeuHis: 2.031 ± 0.032	5.644LeuIle: 5.644 ± 0.065	7.255LeuLys: 7.255 ± 0.068	9.406LeuLeu: 9.406 ± 0.087	2.016LeuMet: 2.016 ± 0.029	5.548LeuAsn: 5.548 ± 0.049	4.417LeuPro: 4.417 ± 0.044	3.873LeuGln: 3.873 ± 0.038	4.374LeuArg: 4.374 ± 0.043	8.516LeuSer: 8.516 ± 0.069	5.174LeuThr: 5.174 ± 0.042	5.645LeuVal: 5.645 ± 0.057	0.863LeuTrp: 0.863 ± 0.022	3.023LeuTyr: 3.023 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	1.383MetAla: 1.383 ± 0.022	0.268MetCys: 0.268 ± 0.009	1.252MetAsp: 1.252 ± 0.022	1.245MetGlu: 1.245 ± 0.025	0.911MetPhe: 0.911 ± 0.019	1.16MetGly: 1.16 ± 0.024	0.401MetHis: 0.401 ± 0.015	1.331MetIle: 1.331 ± 0.023	1.586MetLys: 1.586 ± 0.024	1.966MetLeu: 1.966 ± 0.025	0.553MetMet: 0.553 ± 0.014	1.366MetAsn: 1.366 ± 0.033	0.8MetPro: 0.8 ± 0.019	0.75MetGln: 0.75 ± 0.017	0.883MetArg: 0.883 ± 0.019	2.044MetSer: 2.044 ± 0.029	1.132MetThr: 1.132 ± 0.02	1.257MetVal: 1.257 ± 0.025	0.177MetTrp: 0.177 ± 0.008	0.649MetTyr: 0.649 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.14AsnAla: 3.14 ± 0.041	0.674AsnCys: 0.674 ± 0.021	3.685AsnAsp: 3.685 ± 0.048	3.731AsnGlu: 3.731 ± 0.037	2.499AsnPhe: 2.499 ± 0.035	3.36AsnGly: 3.36 ± 0.047	1.155AsnHis: 1.155 ± 0.024	3.647AsnIle: 3.647 ± 0.04	3.613AsnLys: 3.613 ± 0.043	5.032AsnLeu: 5.032 ± 0.052	1.274AsnMet: 1.274 ± 0.027	3.949AsnAsn: 3.949 ± 0.075	2.512AsnPro: 2.512 ± 0.037	1.878AsnGln: 1.878 ± 0.037	2.14AsnArg: 2.14 ± 0.03	5.511AsnSer: 5.511 ± 0.065	3.197AsnThr: 3.197 ± 0.044	3.235AsnVal: 3.235 ± 0.035	0.576AsnTrp: 0.576 ± 0.014	2.091AsnTyr: 2.091 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	2.999ProAla: 2.999 ± 0.051	0.374ProCys: 0.374 ± 0.013	2.281ProAsp: 2.281 ± 0.032	2.922ProGlu: 2.922 ± 0.04	1.834ProPhe: 1.834 ± 0.028	2.116ProGly: 2.116 ± 0.03	0.928ProHis: 0.928 ± 0.02	2.533ProIle: 2.533 ± 0.033	2.667ProLys: 2.667 ± 0.035	3.978ProLeu: 3.978 ± 0.041	0.782ProMet: 0.782 ± 0.017	2.267ProAsn: 2.267 ± 0.034	2.425ProPro: 2.425 ± 0.064	1.846ProGln: 1.846 ± 0.042	1.645ProArg: 1.645 ± 0.025	4.063ProSer: 4.063 ± 0.056	2.656ProThr: 2.656 ± 0.04	2.735ProVal: 2.735 ± 0.031	0.382ProTrp: 0.382 ± 0.012	1.388ProTyr: 1.388 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	2.077GlnAla: 2.077 ± 0.041	0.409GlnCys: 0.409 ± 0.013	1.706GlnAsp: 1.706 ± 0.028	2.158GlnGlu: 2.158 ± 0.031	1.607GlnPhe: 1.607 ± 0.026	1.625GlnGly: 1.625 ± 0.03	0.979GlnHis: 0.979 ± 0.031	2.341GlnIle: 2.341 ± 0.03	2.563GlnLys: 2.563 ± 0.031	3.972GlnLeu: 3.972 ± 0.041	0.907GlnMet: 0.907 ± 0.025	2.055GlnAsn: 2.055 ± 0.032	1.664GlnPro: 1.664 ± 0.044	3.02GlnGln: 3.02 ± 0.126	1.848GlnArg: 1.848 ± 0.029	2.837GlnSer: 2.837 ± 0.036	1.938GlnThr: 1.938 ± 0.029	1.954GlnVal: 1.954 ± 0.03	0.346GlnTrp: 0.346 ± 0.012	1.327GlnTyr: 1.327 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.548ArgAla: 2.548 ± 0.037	0.589ArgCys: 0.589 ± 0.016	2.258ArgAsp: 2.258 ± 0.032	2.866ArgGlu: 2.866 ± 0.04	1.939ArgPhe: 1.939 ± 0.028	2.356ArgGly: 2.356 ± 0.035	1.027ArgHis: 1.027 ± 0.022	2.644ArgIle: 2.644 ± 0.027	3.494ArgLys: 3.494 ± 0.041	4.347ArgLeu: 4.347 ± 0.04	0.975ArgMet: 0.975 ± 0.019	2.392ArgAsn: 2.392 ± 0.032	1.692ArgPro: 1.692 ± 0.026	1.704ArgGln: 1.704 ± 0.026	2.903ArgArg: 2.903 ± 0.045	3.574ArgSer: 3.574 ± 0.044	2.206ArgThr: 2.206 ± 0.033	2.529ArgVal: 2.529 ± 0.031	0.507ArgTrp: 0.507 ± 0.016	1.53ArgTyr: 1.53 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	5.315SerAla: 5.315 ± 0.056	1.035SerCys: 1.035 ± 0.021	4.892SerAsp: 4.892 ± 0.051	4.843SerGlu: 4.843 ± 0.047	3.964SerPhe: 3.964 ± 0.045	4.936SerGly: 4.936 ± 0.06	1.823SerHis: 1.823 ± 0.03	5.514SerIle: 5.514 ± 0.059	6.257SerLys: 6.257 ± 0.055	8.229SerLeu: 8.229 ± 0.072	1.829SerMet: 1.829 ± 0.026	5.52SerAsn: 5.52 ± 0.065	3.857SerPro: 3.857 ± 0.063	3.073SerGln: 3.073 ± 0.044	3.742SerArg: 3.742 ± 0.048	10.462SerSer: 10.462 ± 0.133	5.607SerThr: 5.607 ± 0.065	5.084SerVal: 5.084 ± 0.057	0.8SerTrp: 0.8 ± 0.018	2.79SerTyr: 2.79 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	3.694ThrAla: 3.694 ± 0.042	0.702ThrCys: 0.702 ± 0.019	3.069ThrAsp: 3.069 ± 0.038	3.241ThrGlu: 3.241 ± 0.036	2.441ThrPhe: 2.441 ± 0.035	3.322ThrGly: 3.322 ± 0.048	1.171ThrHis: 1.171 ± 0.024	3.439ThrIle: 3.439 ± 0.034	3.606ThrLys: 3.606 ± 0.041	5.216ThrLeu: 5.216 ± 0.043	1.085ThrMet: 1.085 ± 0.018	3.031ThrAsn: 3.031 ± 0.036	2.992ThrPro: 2.992 ± 0.042	1.804ThrGln: 1.804 ± 0.028	2.251ThrArg: 2.251 ± 0.029	5.185ThrSer: 5.185 ± 0.066	3.645ThrThr: 3.645 ± 0.065	3.437ThrVal: 3.437 ± 0.041	0.51ThrTrp: 0.51 ± 0.013	1.71ThrTyr: 1.71 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	3.764ValAla: 3.764 ± 0.046	0.904ValCys: 0.904 ± 0.02	3.975ValAsp: 3.975 ± 0.044	4.042ValGlu: 4.042 ± 0.044	2.722ValPhe: 2.722 ± 0.039	3.426ValGly: 3.426 ± 0.04	1.204ValHis: 1.204 ± 0.023	3.448ValIle: 3.448 ± 0.041	4.028ValLys: 4.028 ± 0.04	5.794ValLeu: 5.794 ± 0.048	1.163ValMet: 1.163 ± 0.02	3.088ValAsn: 3.088 ± 0.034	2.771ValPro: 2.771 ± 0.034	2.08ValGln: 2.08 ± 0.029	2.558ValArg: 2.558 ± 0.031	5.295ValSer: 5.295 ± 0.038	3.057ValThr: 3.057 ± 0.037	3.991ValVal: 3.991 ± 0.045	0.646ValTrp: 0.646 ± 0.016	2.07ValTyr: 2.07 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	0.529TrpAla: 0.529 ± 0.017	0.233TrpCys: 0.233 ± 0.01	0.628TrpAsp: 0.628 ± 0.017	0.612TrpGlu: 0.612 ± 0.016	0.51TrpPhe: 0.51 ± 0.017	0.533TrpGly: 0.533 ± 0.014	0.188TrpHis: 0.188 ± 0.009	0.671TrpIle: 0.671 ± 0.018	0.847TrpLys: 0.847 ± 0.019	0.925TrpLeu: 0.925 ± 0.02	0.245TrpMet: 0.245 ± 0.008	0.635TrpAsn: 0.635 ± 0.016	0.301TrpPro: 0.301 ± 0.012	0.3TrpGln: 0.3 ± 0.011	0.552TrpArg: 0.552 ± 0.016	0.807TrpSer: 0.807 ± 0.019	0.522TrpThr: 0.522 ± 0.014	0.61TrpVal: 0.61 ± 0.017	0.165TrpTrp: 0.165 ± 0.008	0.357TrpTyr: 0.357 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.942TyrAla: 1.942 ± 0.029	0.524TyrCys: 0.524 ± 0.014	2.257TyrAsp: 2.257 ± 0.035	2.124TyrGlu: 2.124 ± 0.028	1.672TyrPhe: 1.672 ± 0.028	1.994TyrGly: 1.994 ± 0.031	0.778TyrHis: 0.778 ± 0.016	2.049TyrIle: 2.049 ± 0.034	2.172TyrLys: 2.172 ± 0.028	3.431TyrLeu: 3.431 ± 0.04	0.716TyrMet: 0.716 ± 0.016	1.956TyrAsn: 1.956 ± 0.031	1.42TyrPro: 1.42 ± 0.024	1.3TyrGln: 1.3 ± 0.025	1.482TyrArg: 1.482 ± 0.023	2.853TyrSer: 2.853 ± 0.041	1.853TyrThr: 1.853 ± 0.026	2.058TyrVal: 2.058 ± 0.032	0.409TyrTrp: 0.409 ± 0.014	1.418TyrTyr: 1.418 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5536 proteins (2673506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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