Smittium mucronatum
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R0GMQ3|A0A1R0GMQ3_9FUNG Uncharacterized protein (Fragment) OS=Smittium mucronatum OX=133383 GN=AYI68_g7809 PE=4 SV=1
MM1 pKa = 7.1 QLYY4 pKa = 10.48 APIDD8 pKa = 3.23 ICRR11 pKa = 11.84 VIRR14 pKa = 11.84 EE15 pKa = 4.25 DD16 pKa = 3.45 GVNYY20 pKa = 9.79 EE21 pKa = 4.05 WAINPLKK28 pKa = 10.41 PIIEE32 pKa = 4.24 VSSSNIITYY41 pKa = 9.76 DD42 pKa = 2.9 IHH44 pKa = 7.36 IGKK47 pKa = 9.13 VVRR50 pKa = 11.84 DD51 pKa = 3.61 VTIKK55 pKa = 10.73 FEE57 pKa = 5.48 NDD59 pKa = 3.01 FQPNTDD65 pKa = 3.41 QLVSPLHH72 pKa = 6.32 EE73 pKa = 4.29 NTPYY77 pKa = 10.47 FYY79 pKa = 10.4 LTEE82 pKa = 4.45 TPDD85 pKa = 3.16 SSEE88 pKa = 3.87 FQEE91 pKa = 4.98 NDD93 pKa = 2.93 ILNLNSTEE101 pKa = 4.53 NIDD104 pKa = 5.24 SEE106 pKa = 4.59 DD107 pKa = 3.85 LSDD110 pKa = 3.6 TTEE113 pKa = 3.75 QSSDD117 pKa = 3.5 FNSDD121 pKa = 3.4 PEE123 pKa = 4.27 IYY125 pKa = 8.84 QTDD128 pKa = 3.83 TYY130 pKa = 11.45 YY131 pKa = 11.06 RR132 pKa = 11.84 DD133 pKa = 3.21
Molecular weight: 15.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.897
Dawson 3.77
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.49
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A1R0H183|A0A1R0H183_9FUNG Syntaxin-1A OS=Smittium mucronatum OX=133383 GN=AYI68_g2964 PE=3 SV=1
MM1 pKa = 6.89 SLKK4 pKa = 10.2 VVRR7 pKa = 11.84 HH8 pKa = 4.87 FTQRR12 pKa = 11.84 QMTQRR17 pKa = 11.84 RR18 pKa = 11.84 LIQLIWPNGHH28 pKa = 7.23 FIQKK32 pKa = 9.68 HH33 pKa = 4.73 LANSTHH39 pKa = 6.27
Molecular weight: 4.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 10.73
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7962
0
7962
3195901
8
5138
401.4
45.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.837 ± 0.024
1.164 ± 0.011
5.834 ± 0.022
5.83 ± 0.027
4.686 ± 0.02
5.039 ± 0.029
1.886 ± 0.013
7.089 ± 0.021
7.185 ± 0.032
8.974 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.905 ± 0.011
6.884 ± 0.035
4.9 ± 0.026
3.516 ± 0.02
4.06 ± 0.022
11.485 ± 0.052
5.17 ± 0.026
5.335 ± 0.023
0.805 ± 0.008
3.413 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here