Smittium mucronatum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Legeriomycetaceae; Smittium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7962 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1R0GMQ3|A0A1R0GMQ3_9FUNG Uncharacterized protein (Fragment) OS=Smittium mucronatum OX=133383 GN=AYI68_g7809 PE=4 SV=1
MM1 pKa = 7.1QLYY4 pKa = 10.48APIDD8 pKa = 3.23ICRR11 pKa = 11.84VIRR14 pKa = 11.84EE15 pKa = 4.25DD16 pKa = 3.45GVNYY20 pKa = 9.79EE21 pKa = 4.05WAINPLKK28 pKa = 10.41PIIEE32 pKa = 4.24VSSSNIITYY41 pKa = 9.76DD42 pKa = 2.9IHH44 pKa = 7.36IGKK47 pKa = 9.13VVRR50 pKa = 11.84DD51 pKa = 3.61VTIKK55 pKa = 10.73FEE57 pKa = 5.48NDD59 pKa = 3.01FQPNTDD65 pKa = 3.41QLVSPLHH72 pKa = 6.32EE73 pKa = 4.29NTPYY77 pKa = 10.47FYY79 pKa = 10.4LTEE82 pKa = 4.45TPDD85 pKa = 3.16SSEE88 pKa = 3.87FQEE91 pKa = 4.98NDD93 pKa = 2.93ILNLNSTEE101 pKa = 4.53NIDD104 pKa = 5.24SEE106 pKa = 4.59DD107 pKa = 3.85LSDD110 pKa = 3.6TTEE113 pKa = 3.75QSSDD117 pKa = 3.5FNSDD121 pKa = 3.4PEE123 pKa = 4.27IYY125 pKa = 8.84QTDD128 pKa = 3.83TYY130 pKa = 11.45YY131 pKa = 11.06RR132 pKa = 11.84DD133 pKa = 3.21

Molecular weight:
15.44 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1R0H183|A0A1R0H183_9FUNG Syntaxin-1A OS=Smittium mucronatum OX=133383 GN=AYI68_g2964 PE=3 SV=1
MM1 pKa = 6.89SLKK4 pKa = 10.2VVRR7 pKa = 11.84HH8 pKa = 4.87FTQRR12 pKa = 11.84QMTQRR17 pKa = 11.84RR18 pKa = 11.84LIQLIWPNGHH28 pKa = 7.23FIQKK32 pKa = 9.68HH33 pKa = 4.73LANSTHH39 pKa = 6.27

Molecular weight:
4.75 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7962

0

7962

3195901

8

5138

401.4

45.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.837 ± 0.024

1.164 ± 0.011

5.834 ± 0.022

5.83 ± 0.027

4.686 ± 0.02

5.039 ± 0.029

1.886 ± 0.013

7.089 ± 0.021

7.185 ± 0.032

8.974 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.011

6.884 ± 0.035

4.9 ± 0.026

3.516 ± 0.02

4.06 ± 0.022

11.485 ± 0.052

5.17 ± 0.026

5.335 ± 0.023

0.805 ± 0.008

3.413 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski