Grapevine leafroll-associated virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q49IG7|Q49IG7_9CLOS Major capsid protein OS=Grapevine leafroll-associated virus 2 OX=64003 GN=CP PE=4 SV=1
MM1 pKa = 7.57EE2 pKa = 5.09NYY4 pKa = 9.69EE5 pKa = 4.42EE6 pKa = 4.58KK7 pKa = 10.65SEE9 pKa = 4.32SLVLLRR15 pKa = 11.84TNLNTMLLVVKK26 pKa = 10.44SDD28 pKa = 3.81ASVEE32 pKa = 4.11LPKK35 pKa = 11.01LLICGYY41 pKa = 10.09LRR43 pKa = 11.84VSGRR47 pKa = 11.84GEE49 pKa = 3.92VTCCNRR55 pKa = 11.84EE56 pKa = 3.9DD57 pKa = 3.5LTRR60 pKa = 11.84DD61 pKa = 3.42FEE63 pKa = 5.12GNHH66 pKa = 5.23HH67 pKa = 5.08TVIRR71 pKa = 11.84SRR73 pKa = 11.84TIQYY77 pKa = 10.43DD78 pKa = 3.5SEE80 pKa = 4.71SAFEE84 pKa = 4.17EE85 pKa = 4.82FNNSDD90 pKa = 3.77CVVKK94 pKa = 10.08FFLEE98 pKa = 4.24TGSVFWFFLRR108 pKa = 11.84SEE110 pKa = 4.46TKK112 pKa = 10.11GRR114 pKa = 11.84AVRR117 pKa = 11.84HH118 pKa = 5.33LRR120 pKa = 11.84TFFEE124 pKa = 4.57ANNFFFGSHH133 pKa = 6.82CGTMEE138 pKa = 3.91YY139 pKa = 10.47CLKK142 pKa = 10.58QVLTEE147 pKa = 4.12TEE149 pKa = 4.22SIIDD153 pKa = 3.75SFCEE157 pKa = 3.66EE158 pKa = 4.15RR159 pKa = 11.84NRR161 pKa = 4.3

Molecular weight: 18.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q49IH2|Q49IH2_9CLOS RNA-dependent RNA polymerase (Fragment) OS=Grapevine leafroll-associated virus 2 OX=64003 GN=ORF1b PE=4 SV=1
MM1 pKa = 6.88NQVLQFEE8 pKa = 5.06CLFLLNLAVFAVTFIFILLVIRR30 pKa = 11.84VIKK33 pKa = 10.24SFRR36 pKa = 11.84QKK38 pKa = 10.28SHH40 pKa = 6.12EE41 pKa = 4.1APNPVVRR48 pKa = 11.84SGGFSTVVV56 pKa = 2.74

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5389

56

2936

598.8

67.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.977 ± 0.371

2.691 ± 0.213

5.623 ± 0.292

5.79 ± 0.722

6.235 ± 0.474

5.864 ± 0.51

1.67 ± 0.139

4.676 ± 0.3

6.365 ± 0.354

9.612 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.256

4.008 ± 0.492

2.988 ± 0.18

2.097 ± 0.218

6.495 ± 0.404

9.686 ± 0.601

4.398 ± 0.368

8.777 ± 0.625

0.52 ± 0.235

3.433 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski