Streptomyces phage Issmi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SZL7|A0A6M3SZL7_9CAUD Nuclease OS=Streptomyces phage Issmi OX=2725628 GN=4 PE=4 SV=1
MM1 pKa = 7.3TNIAEE6 pKa = 4.18VGGAITALAGVFGDD20 pKa = 4.88GMTADD25 pKa = 3.61MVGASFTCSEE35 pKa = 4.51ADD37 pKa = 3.33TVARR41 pKa = 11.84LLALAGHH48 pKa = 6.72KK49 pKa = 10.32SEE51 pKa = 4.59ALTWLEE57 pKa = 3.54GHH59 pKa = 6.35AQGDD63 pKa = 4.23EE64 pKa = 4.31VDD66 pKa = 4.34DD67 pKa = 3.46SHH69 pKa = 5.98YY70 pKa = 10.04TYY72 pKa = 11.19DD73 pKa = 3.43EE74 pKa = 4.32TDD76 pKa = 3.65PEE78 pKa = 4.47DD79 pKa = 3.48EE80 pKa = 4.36GRR82 pKa = 11.84VLTEE86 pKa = 4.07AEE88 pKa = 3.96LVEE91 pKa = 4.53YY92 pKa = 10.57IEE94 pKa = 5.16GLTAA98 pKa = 5.05

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SZT8|A0A6M3SZT8_9CAUD Uncharacterized protein OS=Streptomyces phage Issmi OX=2725628 GN=74 PE=4 SV=1
MM1 pKa = 7.71PNWQGSDD8 pKa = 2.91RR9 pKa = 11.84RR10 pKa = 11.84SRR12 pKa = 11.84LPADD16 pKa = 3.29WPKK19 pKa = 10.44IRR21 pKa = 11.84LRR23 pKa = 11.84VLRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.54GGQCTALTEE37 pKa = 4.34AGEE40 pKa = 4.51RR41 pKa = 11.84CDD43 pKa = 3.9STATDD48 pKa = 3.35VDD50 pKa = 4.42HH51 pKa = 7.22IRR53 pKa = 11.84PGDD56 pKa = 3.68DD57 pKa = 2.82HH58 pKa = 9.31SMGNLRR64 pKa = 11.84SLCSWHH70 pKa = 6.68HH71 pKa = 5.1RR72 pKa = 11.84QKK74 pKa = 10.9SSRR77 pKa = 11.84EE78 pKa = 3.88GAEE81 pKa = 3.54AAHH84 pKa = 6.15ARR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84AIQKK92 pKa = 9.55RR93 pKa = 11.84FRR95 pKa = 11.84RR96 pKa = 11.84DD97 pKa = 2.93EE98 pKa = 3.97AHH100 pKa = 6.96PGLMM104 pKa = 4.56

Molecular weight: 11.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15371

45

1360

194.6

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.281 ± 0.291

0.735 ± 0.113

6.558 ± 0.249

6.668 ± 0.423

3.032 ± 0.191

8.262 ± 0.314

2.082 ± 0.202

4.144 ± 0.262

4.548 ± 0.361

8.516 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.162

2.745 ± 0.114

4.866 ± 0.238

3.585 ± 0.175

6.519 ± 0.424

6.037 ± 0.306

6.707 ± 0.353

7.013 ± 0.237

1.835 ± 0.116

2.752 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski