Amino acid dipepetide frequency for Streptomyces phage Issmi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.646AlaAla: 11.646 ± 1.203
0.52AlaCys: 0.52 ± 0.2
8.393AlaAsp: 8.393 ± 0.666
8.198AlaGlu: 8.198 ± 0.917
2.928AlaPhe: 2.928 ± 0.51
9.694AlaGly: 9.694 ± 0.925
2.082AlaHis: 2.082 ± 0.476
4.684AlaIle: 4.684 ± 0.642
5.725AlaLys: 5.725 ± 0.771
11.386AlaLeu: 11.386 ± 1.389
2.863AlaMet: 2.863 ± 0.514
3.058AlaAsn: 3.058 ± 0.454
4.619AlaPro: 4.619 ± 0.674
3.643AlaGln: 3.643 ± 0.541
7.352AlaArg: 7.352 ± 0.868
5.465AlaSer: 5.465 ± 0.606
6.571AlaThr: 6.571 ± 0.646
8.068AlaVal: 8.068 ± 0.834
2.602AlaTrp: 2.602 ± 0.432
3.318AlaTyr: 3.318 ± 0.551
0.0AlaXaa: 0.0 ± 0.0
Cys
0.586CysAla: 0.586 ± 0.164
0.065CysCys: 0.065 ± 0.061
0.781CysAsp: 0.781 ± 0.221
0.455CysGlu: 0.455 ± 0.166
0.325CysPhe: 0.325 ± 0.136
0.586CysGly: 0.586 ± 0.194
0.26CysHis: 0.26 ± 0.141
0.39CysIle: 0.39 ± 0.176
0.325CysLys: 0.325 ± 0.18
0.455CysLeu: 0.455 ± 0.164
0.0CysMet: 0.0 ± 0.0
0.065CysAsn: 0.065 ± 0.064
0.455CysPro: 0.455 ± 0.238
0.065CysGln: 0.065 ± 0.055
0.39CysArg: 0.39 ± 0.164
0.716CysSer: 0.716 ± 0.241
0.52CysThr: 0.52 ± 0.216
0.13CysVal: 0.13 ± 0.116
0.39CysTrp: 0.39 ± 0.185
0.39CysTyr: 0.39 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
6.701AspAla: 6.701 ± 0.665
0.52AspCys: 0.52 ± 0.197
4.229AspAsp: 4.229 ± 0.578
5.01AspGlu: 5.01 ± 0.683
2.147AspPhe: 2.147 ± 0.398
6.506AspGly: 6.506 ± 0.592
1.171AspHis: 1.171 ± 0.359
2.668AspIle: 2.668 ± 0.439
2.082AspLys: 2.082 ± 0.333
6.376AspLeu: 6.376 ± 0.659
1.692AspMet: 1.692 ± 0.318
2.082AspAsn: 2.082 ± 0.287
4.229AspPro: 4.229 ± 0.635
2.407AspGln: 2.407 ± 0.462
3.513AspArg: 3.513 ± 0.586
3.709AspSer: 3.709 ± 0.473
3.253AspThr: 3.253 ± 0.441
4.229AspVal: 4.229 ± 0.418
1.496AspTrp: 1.496 ± 0.299
1.561AspTyr: 1.561 ± 0.279
0.0AspXaa: 0.0 ± 0.0
Glu
8.263GluAla: 8.263 ± 0.925
0.846GluCys: 0.846 ± 0.292
3.578GluAsp: 3.578 ± 0.399
4.75GluGlu: 4.75 ± 0.776
1.822GluPhe: 1.822 ± 0.37
5.725GluGly: 5.725 ± 0.666
1.366GluHis: 1.366 ± 0.335
2.993GluIle: 2.993 ± 0.432
1.952GluLys: 1.952 ± 0.337
7.092GluLeu: 7.092 ± 0.714
1.236GluMet: 1.236 ± 0.239
1.366GluAsn: 1.366 ± 0.269
3.058GluPro: 3.058 ± 0.499
3.318GluGln: 3.318 ± 0.473
3.643GluArg: 3.643 ± 0.566
3.188GluSer: 3.188 ± 0.47
3.904GluThr: 3.904 ± 0.712
4.88GluVal: 4.88 ± 0.616
1.496GluTrp: 1.496 ± 0.352
2.212GluTyr: 2.212 ± 0.427
0.0GluXaa: 0.0 ± 0.0
Phe
3.513PheAla: 3.513 ± 0.534
0.39PheCys: 0.39 ± 0.146
2.342PheAsp: 2.342 ± 0.308
2.082PheGlu: 2.082 ± 0.409
0.976PhePhe: 0.976 ± 0.438
2.863PheGly: 2.863 ± 0.38
0.455PheHis: 0.455 ± 0.182
0.976PheIle: 0.976 ± 0.232
1.041PheLys: 1.041 ± 0.323
2.212PheLeu: 2.212 ± 0.347
0.586PheMet: 0.586 ± 0.21
1.171PheAsn: 1.171 ± 0.296
1.171PhePro: 1.171 ± 0.338
1.301PheGln: 1.301 ± 0.325
1.887PheArg: 1.887 ± 0.339
1.952PheSer: 1.952 ± 0.403
2.147PheThr: 2.147 ± 0.345
2.147PheVal: 2.147 ± 0.442
0.39PheTrp: 0.39 ± 0.117
0.716PheTyr: 0.716 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
7.482GlyAla: 7.482 ± 0.926
0.455GlyCys: 0.455 ± 0.211
5.66GlyAsp: 5.66 ± 0.708
5.205GlyGlu: 5.205 ± 0.541
3.123GlyPhe: 3.123 ± 0.597
5.4GlyGly: 5.4 ± 0.624
2.277GlyHis: 2.277 ± 0.382
3.383GlyIle: 3.383 ± 0.652
5.14GlyLys: 5.14 ± 0.711
6.897GlyLeu: 6.897 ± 0.877
1.301GlyMet: 1.301 ± 0.244
2.212GlyAsn: 2.212 ± 0.29
3.383GlyPro: 3.383 ± 0.42
2.863GlyGln: 2.863 ± 0.547
5.01GlyArg: 5.01 ± 0.742
5.335GlySer: 5.335 ± 0.719
6.636GlyThr: 6.636 ± 0.829
6.181GlyVal: 6.181 ± 0.642
2.342GlyTrp: 2.342 ± 0.424
2.342GlyTyr: 2.342 ± 0.429
0.0GlyXaa: 0.0 ± 0.0
His
2.082HisAla: 2.082 ± 0.358
0.195HisCys: 0.195 ± 0.112
1.041HisAsp: 1.041 ± 0.245
1.366HisGlu: 1.366 ± 0.325
1.106HisPhe: 1.106 ± 0.248
1.496HisGly: 1.496 ± 0.338
1.041HisHis: 1.041 ± 0.274
1.236HisIle: 1.236 ± 0.266
0.39HisLys: 0.39 ± 0.154
1.431HisLeu: 1.431 ± 0.305
0.39HisMet: 0.39 ± 0.14
0.586HisAsn: 0.586 ± 0.207
1.106HisPro: 1.106 ± 0.287
0.781HisGln: 0.781 ± 0.273
1.496HisArg: 1.496 ± 0.436
1.431HisSer: 1.431 ± 0.361
1.041HisThr: 1.041 ± 0.264
1.431HisVal: 1.431 ± 0.381
0.586HisTrp: 0.586 ± 0.222
0.586HisTyr: 0.586 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
5.14IleAla: 5.14 ± 0.563
0.26IleCys: 0.26 ± 0.125
3.969IleAsp: 3.969 ± 0.483
3.383IleGlu: 3.383 ± 0.518
0.976IlePhe: 0.976 ± 0.224
3.253IleGly: 3.253 ± 0.573
0.976IleHis: 0.976 ± 0.245
1.366IleIle: 1.366 ± 0.545
1.887IleLys: 1.887 ± 0.518
3.318IleLeu: 3.318 ± 0.5
0.39IleMet: 0.39 ± 0.209
1.041IleAsn: 1.041 ± 0.23
1.757IlePro: 1.757 ± 0.328
1.171IleGln: 1.171 ± 0.245
2.863IleArg: 2.863 ± 0.435
1.887IleSer: 1.887 ± 0.547
3.058IleThr: 3.058 ± 0.503
3.383IleVal: 3.383 ± 0.526
0.26IleTrp: 0.26 ± 0.117
1.106IleTyr: 1.106 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
5.335LysAla: 5.335 ± 1.054
0.26LysCys: 0.26 ± 0.195
2.798LysAsp: 2.798 ± 0.485
2.212LysGlu: 2.212 ± 0.391
1.041LysPhe: 1.041 ± 0.201
4.359LysGly: 4.359 ± 0.776
0.846LysHis: 0.846 ± 0.256
2.082LysIle: 2.082 ± 0.562
2.798LysLys: 2.798 ± 0.65
4.229LysLeu: 4.229 ± 0.721
0.781LysMet: 0.781 ± 0.196
0.976LysAsn: 0.976 ± 0.26
2.733LysPro: 2.733 ± 0.632
1.757LysGln: 1.757 ± 0.397
3.578LysArg: 3.578 ± 0.53
2.082LysSer: 2.082 ± 0.439
2.472LysThr: 2.472 ± 0.41
2.993LysVal: 2.993 ± 0.567
0.325LysTrp: 0.325 ± 0.174
1.822LysTyr: 1.822 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
10.475LeuAla: 10.475 ± 0.932
0.586LeuCys: 0.586 ± 0.181
5.465LeuAsp: 5.465 ± 0.531
4.229LeuGlu: 4.229 ± 0.538
2.082LeuPhe: 2.082 ± 0.597
8.003LeuGly: 8.003 ± 1.422
1.431LeuHis: 1.431 ± 0.367
3.578LeuIle: 3.578 ± 0.463
4.099LeuLys: 4.099 ± 0.992
7.027LeuLeu: 7.027 ± 0.704
1.822LeuMet: 1.822 ± 0.375
3.253LeuAsn: 3.253 ± 0.415
4.294LeuPro: 4.294 ± 0.654
2.798LeuGln: 2.798 ± 0.432
5.4LeuArg: 5.4 ± 0.905
5.791LeuSer: 5.791 ± 0.917
6.376LeuThr: 6.376 ± 0.638
5.53LeuVal: 5.53 ± 0.674
1.106LeuTrp: 1.106 ± 0.246
1.822LeuTyr: 1.822 ± 0.325
0.0LeuXaa: 0.0 ± 0.0
Met
3.448MetAla: 3.448 ± 0.456
0.0MetCys: 0.0 ± 0.0
0.976MetAsp: 0.976 ± 0.277
1.106MetGlu: 1.106 ± 0.297
0.52MetPhe: 0.52 ± 0.204
1.366MetGly: 1.366 ± 0.309
0.781MetHis: 0.781 ± 0.218
0.716MetIle: 0.716 ± 0.196
0.651MetLys: 0.651 ± 0.206
1.757MetLeu: 1.757 ± 0.383
0.455MetMet: 0.455 ± 0.178
0.39MetAsn: 0.39 ± 0.161
1.301MetPro: 1.301 ± 0.299
0.455MetGln: 0.455 ± 0.153
1.561MetArg: 1.561 ± 0.39
2.017MetSer: 2.017 ± 0.367
2.147MetThr: 2.147 ± 0.359
0.911MetVal: 0.911 ± 0.197
0.325MetTrp: 0.325 ± 0.132
0.26MetTyr: 0.26 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
2.733AsnAla: 2.733 ± 0.457
0.39AsnCys: 0.39 ± 0.185
1.561AsnAsp: 1.561 ± 0.339
1.692AsnGlu: 1.692 ± 0.332
0.846AsnPhe: 0.846 ± 0.198
3.058AsnGly: 3.058 ± 0.52
0.52AsnHis: 0.52 ± 0.186
1.496AsnIle: 1.496 ± 0.222
1.106AsnLys: 1.106 ± 0.273
2.082AsnLeu: 2.082 ± 0.383
0.39AsnMet: 0.39 ± 0.183
0.846AsnAsn: 0.846 ± 0.249
1.822AsnPro: 1.822 ± 0.333
0.846AsnGln: 0.846 ± 0.212
1.692AsnArg: 1.692 ± 0.284
1.431AsnSer: 1.431 ± 0.288
1.887AsnThr: 1.887 ± 0.37
2.017AsnVal: 2.017 ± 0.287
0.52AsnTrp: 0.52 ± 0.148
0.52AsnTyr: 0.52 ± 0.157
0.0AsnXaa: 0.0 ± 0.0
Pro
6.051ProAla: 6.051 ± 0.885
0.586ProCys: 0.586 ± 0.195
3.774ProAsp: 3.774 ± 0.598
4.164ProGlu: 4.164 ± 0.592
1.106ProPhe: 1.106 ± 0.254
4.164ProGly: 4.164 ± 0.436
0.651ProHis: 0.651 ± 0.258
1.627ProIle: 1.627 ± 0.319
2.602ProLys: 2.602 ± 0.538
3.318ProLeu: 3.318 ± 0.424
1.301ProMet: 1.301 ± 0.299
0.781ProAsn: 0.781 ± 0.184
1.952ProPro: 1.952 ± 0.353
1.887ProGln: 1.887 ± 0.343
2.407ProArg: 2.407 ± 0.41
3.123ProSer: 3.123 ± 0.638
3.253ProThr: 3.253 ± 0.491
3.709ProVal: 3.709 ± 0.458
0.911ProTrp: 0.911 ± 0.259
1.301ProTyr: 1.301 ± 0.29
0.0ProXaa: 0.0 ± 0.0
Gln
5.856GlnAla: 5.856 ± 0.75
0.195GlnCys: 0.195 ± 0.126
1.431GlnAsp: 1.431 ± 0.304
2.277GlnGlu: 2.277 ± 0.478
1.236GlnPhe: 1.236 ± 0.313
2.277GlnGly: 2.277 ± 0.297
0.39GlnHis: 0.39 ± 0.18
1.561GlnIle: 1.561 ± 0.457
1.692GlnLys: 1.692 ± 0.36
2.602GlnLeu: 2.602 ± 0.798
1.366GlnMet: 1.366 ± 0.335
0.911GlnAsn: 0.911 ± 0.222
1.041GlnPro: 1.041 ± 0.269
0.911GlnGln: 0.911 ± 0.247
2.537GlnArg: 2.537 ± 0.388
2.082GlnSer: 2.082 ± 0.323
2.277GlnThr: 2.277 ± 0.371
3.709GlnVal: 3.709 ± 0.442
0.651GlnTrp: 0.651 ± 0.164
0.846GlnTyr: 0.846 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
6.506ArgAla: 6.506 ± 0.93
0.455ArgCys: 0.455 ± 0.181
4.034ArgAsp: 4.034 ± 0.574
4.424ArgGlu: 4.424 ± 0.825
2.342ArgPhe: 2.342 ± 0.419
4.359ArgGly: 4.359 ± 0.622
1.692ArgHis: 1.692 ± 0.368
2.277ArgIle: 2.277 ± 0.373
3.643ArgLys: 3.643 ± 0.438
5.856ArgLeu: 5.856 ± 0.773
1.561ArgMet: 1.561 ± 0.315
1.692ArgAsn: 1.692 ± 0.355
3.253ArgPro: 3.253 ± 0.457
2.798ArgGln: 2.798 ± 0.457
5.66ArgArg: 5.66 ± 1.002
4.229ArgSer: 4.229 ± 0.588
3.253ArgThr: 3.253 ± 0.548
4.099ArgVal: 4.099 ± 0.443
0.716ArgTrp: 0.716 ± 0.303
2.342ArgTyr: 2.342 ± 0.44
0.0ArgXaa: 0.0 ± 0.0
Ser
6.831SerAla: 6.831 ± 0.625
0.39SerCys: 0.39 ± 0.191
3.969SerAsp: 3.969 ± 0.56
3.318SerGlu: 3.318 ± 0.5
1.887SerPhe: 1.887 ± 0.325
4.945SerGly: 4.945 ± 0.594
1.301SerHis: 1.301 ± 0.362
2.668SerIle: 2.668 ± 0.366
2.928SerLys: 2.928 ± 0.436
5.595SerLeu: 5.595 ± 0.865
1.366SerMet: 1.366 ± 0.318
1.692SerAsn: 1.692 ± 0.397
2.863SerPro: 2.863 ± 0.339
2.212SerGln: 2.212 ± 0.446
3.774SerArg: 3.774 ± 0.591
4.034SerSer: 4.034 ± 0.787
4.619SerThr: 4.619 ± 0.725
3.448SerVal: 3.448 ± 0.518
1.106SerTrp: 1.106 ± 0.273
1.366SerTyr: 1.366 ± 0.293
0.0SerXaa: 0.0 ± 0.0
Thr
7.742ThrAla: 7.742 ± 0.833
0.39ThrCys: 0.39 ± 0.169
3.774ThrAsp: 3.774 ± 0.518
4.684ThrGlu: 4.684 ± 0.628
2.472ThrPhe: 2.472 ± 0.42
5.53ThrGly: 5.53 ± 0.598
0.976ThrHis: 0.976 ± 0.259
2.928ThrIle: 2.928 ± 0.469
1.952ThrLys: 1.952 ± 0.316
4.75ThrLeu: 4.75 ± 0.6
0.911ThrMet: 0.911 ± 0.229
1.366ThrAsn: 1.366 ± 0.386
4.164ThrPro: 4.164 ± 0.647
2.602ThrGln: 2.602 ± 0.435
3.904ThrArg: 3.904 ± 0.51
5.335ThrSer: 5.335 ± 0.848
4.359ThrThr: 4.359 ± 0.646
5.53ThrVal: 5.53 ± 0.544
1.171ThrTrp: 1.171 ± 0.252
2.537ThrTyr: 2.537 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
7.222ValAla: 7.222 ± 0.595
0.52ValCys: 0.52 ± 0.164
3.904ValAsp: 3.904 ± 0.5
4.88ValGlu: 4.88 ± 0.609
2.147ValPhe: 2.147 ± 0.334
5.335ValGly: 5.335 ± 0.594
1.496ValHis: 1.496 ± 0.296
3.643ValIle: 3.643 ± 0.546
3.643ValLys: 3.643 ± 0.427
5.725ValLeu: 5.725 ± 0.664
1.561ValMet: 1.561 ± 0.263
2.017ValAsn: 2.017 ± 0.393
3.318ValPro: 3.318 ± 0.38
2.798ValGln: 2.798 ± 0.391
4.619ValArg: 4.619 ± 0.552
3.578ValSer: 3.578 ± 0.515
5.856ValThr: 5.856 ± 0.595
4.945ValVal: 4.945 ± 0.486
1.431ValTrp: 1.431 ± 0.277
1.496ValTyr: 1.496 ± 0.304
0.0ValXaa: 0.0 ± 0.0
Trp
2.017TrpAla: 2.017 ± 0.372
0.26TrpCys: 0.26 ± 0.105
1.431TrpAsp: 1.431 ± 0.306
1.041TrpGlu: 1.041 ± 0.221
0.651TrpPhe: 0.651 ± 0.22
1.106TrpGly: 1.106 ± 0.322
0.455TrpHis: 0.455 ± 0.183
0.325TrpIle: 0.325 ± 0.149
0.911TrpLys: 0.911 ± 0.196
1.496TrpLeu: 1.496 ± 0.34
0.586TrpMet: 0.586 ± 0.178
0.911TrpAsn: 0.911 ± 0.206
0.846TrpPro: 0.846 ± 0.257
0.52TrpGln: 0.52 ± 0.185
1.692TrpArg: 1.692 ± 0.277
1.171TrpSer: 1.171 ± 0.284
1.692TrpThr: 1.692 ± 0.456
0.846TrpVal: 0.846 ± 0.316
0.065TrpTrp: 0.065 ± 0.063
0.325TrpTyr: 0.325 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.188TyrAla: 3.188 ± 0.481
0.065TyrCys: 0.065 ± 0.077
2.472TyrAsp: 2.472 ± 0.384
2.212TyrGlu: 2.212 ± 0.418
0.586TyrPhe: 0.586 ± 0.198
2.602TyrGly: 2.602 ± 0.501
0.651TyrHis: 0.651 ± 0.236
0.846TyrIle: 0.846 ± 0.222
0.911TyrLys: 0.911 ± 0.238
1.561TyrLeu: 1.561 ± 0.341
0.52TyrMet: 0.52 ± 0.186
1.041TyrAsn: 1.041 ± 0.234
1.301TyrPro: 1.301 ± 0.318
0.716TyrGln: 0.716 ± 0.224
2.212TyrArg: 2.212 ± 0.431
1.822TyrSer: 1.822 ± 0.361
1.757TyrThr: 1.757 ± 0.331
1.952TyrVal: 1.952 ± 0.347
0.455TyrTrp: 0.455 ± 0.209
0.651TyrTyr: 0.651 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski