Murine coronavirus repA59/RJHM

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Murine coronavirus; Murine hepatitis virus

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0KYU0|C0KYU0_9BETC Spike glycoprotein OS=Murine coronavirus repA59/RJHM OX=429331 GN=S PE=3 SV=1
MM1 pKa = 7.38SNKK4 pKa = 10.14SILVVAVKK12 pKa = 9.54MAFADD17 pKa = 4.0KK18 pKa = 10.12PNHH21 pKa = 6.87FINFPLAQFSGFMGKK36 pKa = 8.01YY37 pKa = 10.17LKK39 pKa = 10.62LQSQLVEE46 pKa = 4.08MGLDD50 pKa = 3.76CKK52 pKa = 10.5LQKK55 pKa = 10.45APHH58 pKa = 5.48VSITLLDD65 pKa = 3.72IKK67 pKa = 10.77ADD69 pKa = 3.42QYY71 pKa = 11.35KK72 pKa = 9.6QVEE75 pKa = 4.2FAIQEE80 pKa = 4.36IIDD83 pKa = 4.13DD84 pKa = 4.34LAAYY88 pKa = 9.61EE89 pKa = 4.09GDD91 pKa = 3.2IVFDD95 pKa = 3.88NPHH98 pKa = 5.62MLGRR102 pKa = 11.84CLVLDD107 pKa = 3.35VRR109 pKa = 11.84GFEE112 pKa = 4.33EE113 pKa = 3.94LHH115 pKa = 6.12EE116 pKa = 5.4DD117 pKa = 2.78IVEE120 pKa = 3.86ILRR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84GCTADD130 pKa = 4.2QSRR133 pKa = 11.84HH134 pKa = 5.63WIPHH138 pKa = 4.64CTVAQFDD145 pKa = 4.22EE146 pKa = 4.22EE147 pKa = 5.07RR148 pKa = 11.84EE149 pKa = 4.25TKK151 pKa = 10.47GMQFYY156 pKa = 10.52HH157 pKa = 7.09KK158 pKa = 10.33EE159 pKa = 3.82PFYY162 pKa = 11.1LKK164 pKa = 10.56HH165 pKa = 6.52NNLLTDD171 pKa = 3.99AGLEE175 pKa = 4.01LVKK178 pKa = 10.57IGSSKK183 pKa = 10.27IDD185 pKa = 3.21GFYY188 pKa = 10.77CSEE191 pKa = 3.98LSVWCGEE198 pKa = 4.13RR199 pKa = 11.84LCYY202 pKa = 10.09KK203 pKa = 10.34PPTPKK208 pKa = 10.32FSDD211 pKa = 2.77IFGYY215 pKa = 10.69CCIDD219 pKa = 4.15KK220 pKa = 10.66IRR222 pKa = 11.84GDD224 pKa = 4.16LEE226 pKa = 5.63IGDD229 pKa = 4.82LPQDD233 pKa = 4.71DD234 pKa = 4.12EE235 pKa = 4.54EE236 pKa = 5.09AWAEE240 pKa = 3.96LSYY243 pKa = 10.57HH244 pKa = 4.98YY245 pKa = 10.55QRR247 pKa = 11.84NTYY250 pKa = 9.02FFRR253 pKa = 11.84HH254 pKa = 4.59VHH256 pKa = 6.39DD257 pKa = 3.67NSIYY261 pKa = 9.71FRR263 pKa = 11.84TVCRR267 pKa = 11.84MKK269 pKa = 11.07GCMCC273 pKa = 5.37

Molecular weight: 31.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0KYU2|C0KYU2_9BETC Non-structural protein ns5 OS=Murine coronavirus repA59/RJHM OX=429331 PE=4 SV=1
MM1 pKa = 7.46ALIGPKK7 pKa = 7.57TTIAAVFIGPFLVACMLGIGLVYY30 pKa = 10.81LLQLQVQIFHH40 pKa = 6.45VKK42 pKa = 9.07DD43 pKa = 3.89TIRR46 pKa = 11.84VTGKK50 pKa = 9.2PATVSYY56 pKa = 7.5TTSTPVTPVATTLDD70 pKa = 3.53GTTYY74 pKa = 10.5TLIRR78 pKa = 11.84PTSSYY83 pKa = 9.16TRR85 pKa = 11.84VYY87 pKa = 10.18LGSSRR92 pKa = 11.84GFDD95 pKa = 2.9TSTFGPKK102 pKa = 9.08TLDD105 pKa = 3.78YY106 pKa = 9.39ITSSKK111 pKa = 10.15PHH113 pKa = 6.34LNSGRR118 pKa = 11.84PYY120 pKa = 10.28TLRR123 pKa = 11.84HH124 pKa = 5.44LPKK127 pKa = 10.79YY128 pKa = 6.04MTPPATWRR136 pKa = 11.84FGLL139 pKa = 4.06

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

10005

88

4470

1000.5

111.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.386

3.758 ± 0.515

5.537 ± 0.365

4.178 ± 0.313

5.247 ± 0.267

6.217 ± 0.535

1.719 ± 0.282

4.458 ± 0.581

5.737 ± 0.382

9.175 ± 0.538

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.262

5.087 ± 0.722

3.968 ± 0.671

3.518 ± 0.684

3.878 ± 0.513

7.046 ± 0.309

5.837 ± 0.483

9.475 ± 1.274

1.299 ± 0.12

4.758 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski