Oceanobacillus profundus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417YB89|A0A417YB89_9BACI DNA-binding response regulator OS=Oceanobacillus profundus OX=372463 GN=D1B32_19015 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.13KK3 pKa = 9.49ILSIFVFVSIFMIIAACNNTSNSGPAGEE31 pKa = 4.84SGNAEE36 pKa = 3.89DD37 pKa = 4.64VLDD40 pKa = 4.47GEE42 pKa = 5.71FITILTGGSSGVYY55 pKa = 9.41YY56 pKa = 10.25PLGGTLANIFQDD68 pKa = 3.95LGANANSQSTAASTANATTLNQGNAEE94 pKa = 4.33IGFSMGDD101 pKa = 3.39AAQDD105 pKa = 3.36AYY107 pKa = 10.64EE108 pKa = 4.61GVASATEE115 pKa = 3.75IGAQEE120 pKa = 4.38NIRR123 pKa = 11.84TLASLYY129 pKa = 10.12PNYY132 pKa = 10.2LQIVATEE139 pKa = 3.9SSGIKK144 pKa = 9.0TVEE147 pKa = 3.8DD148 pKa = 3.57LAGKK152 pKa = 9.58RR153 pKa = 11.84VAVGAPASGTEE164 pKa = 3.74ISAQRR169 pKa = 11.84VLEE172 pKa = 4.53AYY174 pKa = 10.32GMTYY178 pKa = 11.21DD179 pKa = 6.03DD180 pKa = 4.42INADD184 pKa = 3.46FLSFSEE190 pKa = 4.47GVEE193 pKa = 4.48GIQNGNIDD201 pKa = 3.86AVVISSGLPNAGVLEE216 pKa = 4.45LQTTQDD222 pKa = 3.51VVIVEE227 pKa = 4.04IAEE230 pKa = 4.42DD231 pKa = 4.0IILGMQEE238 pKa = 4.9DD239 pKa = 4.94YY240 pKa = 11.29PSFFPTTVPKK250 pKa = 10.38DD251 pKa = 3.31IYY253 pKa = 11.56DD254 pKa = 3.7MEE256 pKa = 5.56EE257 pKa = 4.01DD258 pKa = 3.58VSTIGVNNVLLTHH271 pKa = 6.15SEE273 pKa = 4.16VSDD276 pKa = 3.48DD277 pKa = 3.48MAYY280 pKa = 10.9AMAKK284 pKa = 10.32AIFDD288 pKa = 4.89NLDD291 pKa = 3.26QLQNTHH297 pKa = 5.92NAANDD302 pKa = 3.31ITLEE306 pKa = 4.02NALQNLPAPLHH317 pKa = 6.29PGAAKK322 pKa = 10.35YY323 pKa = 10.38FEE325 pKa = 4.33EE326 pKa = 4.84QGITEE331 pKa = 4.08

Molecular weight: 34.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417YHC3|A0A417YHC3_9BACI Mechanosensitive ion channel family protein OS=Oceanobacillus profundus OX=372463 GN=D1B32_10815 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.08NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4512

0

4512

1318155

21

2384

292.1

32.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.11 ± 0.035

0.64 ± 0.011

5.33 ± 0.04

7.627 ± 0.047

4.532 ± 0.034

6.803 ± 0.038

2.059 ± 0.015

8.261 ± 0.04

6.538 ± 0.04

9.52 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.795 ± 0.017

4.724 ± 0.028

3.519 ± 0.021

3.725 ± 0.026

3.891 ± 0.025

5.934 ± 0.025

5.547 ± 0.024

6.857 ± 0.03

1.012 ± 0.014

3.575 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski