Pasteurella bettyae CCUG 2042

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurella; Pasteurella bettyae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3DJA9|I3DJA9_9PAST UPF0301 protein HMPREF1052_1086 OS=Pasteurella bettyae CCUG 2042 OX=1095749 GN=HMPREF1052_1086 PE=3 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.06FVARR7 pKa = 11.84FYY9 pKa = 11.09CLVEE13 pKa = 3.92AVVEE17 pKa = 4.25AEE19 pKa = 4.22SNEE22 pKa = 4.1QVLEE26 pKa = 4.2LCDD29 pKa = 5.8LNVCDD34 pKa = 5.13VNKK37 pKa = 10.32LPHH40 pKa = 7.14TITEE44 pKa = 3.78IDD46 pKa = 3.66DD47 pKa = 3.71VVEE50 pKa = 4.21VEE52 pKa = 4.47EE53 pKa = 4.37VV54 pKa = 2.94

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3D6A6|I3D6A6_9PAST Competence protein A domain protein OS=Pasteurella bettyae CCUG 2042 OX=1095749 GN=HMPREF1052_0672 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2177

0

2177

666811

37

1569

306.3

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.07 ± 0.055

1.045 ± 0.02

5.054 ± 0.035

6.296 ± 0.054

4.367 ± 0.04

6.58 ± 0.056

2.119 ± 0.024

7.368 ± 0.052

6.399 ± 0.045

10.399 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.021

4.944 ± 0.045

3.639 ± 0.031

4.688 ± 0.048

4.248 ± 0.039

6.015 ± 0.037

5.343 ± 0.036

6.526 ± 0.046

1.124 ± 0.018

3.288 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski